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Field‐deployable, ultrasensitive and visual detection systems targeting severe fever with thrombocytopenia syndrome virus (Dabie bandavirus) based on CRISPR/Cas12a and DNAzyme.

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Název: Field‐deployable, ultrasensitive and visual detection systems targeting severe fever with thrombocytopenia syndrome virus (Dabie bandavirus) based on CRISPR/Cas12a and DNAzyme.
Autoři: Wang, Junhu, Yue, Ming, Mao, Yingqing, Chen, Qiong, Ge, Yixin, Yi, Haiming, Wang, Hongming, Yang, Sunjie, Zhu, Jin, Li, Yuexi, Qi, Yong
Zdroj: Clinical & Translational Medicine; Jul2025, Vol. 15 Issue 7, p1-6, 6p
Témata: CRISPRS, DEOXYRIBOZYMES, VIRUS diseases, OPTICAL signal detection, DETECTION algorithms
Abstrakt: The article focuses on the development of two innovative CRISPR-based visual detection systems for severe fever with thrombocytopenia syndrome virus (SFTSV), namely RAA-CRISPR/Cas12a-fluorescent (RCCF) and RAA-CRISPR/Cas12a-DNAzyme (RCCD). These systems utilize recombinase-aided amplification (RAA) combined with CRISPR/Cas12a to achieve ultrasensitive and specific detection of SFTSV RNA, addressing limitations of existing detection methods like ELISA and RT-PCR. The RCCF system is designed for laboratory use with fluorescence detection, while the RCCD system is suitable for resource-limited settings, providing colorimetric results visible to the naked eye. Both methods demonstrate high sensitivity and specificity, making them promising tools for SFTSV detection in various scenarios. [Extracted from the article]
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Databáze: Complementary Index
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Abstrakt:The article focuses on the development of two innovative CRISPR-based visual detection systems for severe fever with thrombocytopenia syndrome virus (SFTSV), namely RAA-CRISPR/Cas12a-fluorescent (RCCF) and RAA-CRISPR/Cas12a-DNAzyme (RCCD). These systems utilize recombinase-aided amplification (RAA) combined with CRISPR/Cas12a to achieve ultrasensitive and specific detection of SFTSV RNA, addressing limitations of existing detection methods like ELISA and RT-PCR. The RCCF system is designed for laboratory use with fluorescence detection, while the RCCD system is suitable for resource-limited settings, providing colorimetric results visible to the naked eye. Both methods demonstrate high sensitivity and specificity, making them promising tools for SFTSV detection in various scenarios. [Extracted from the article]
ISSN:20011326
DOI:10.1002/ctm2.70393