Real-time monitoring by interferometric light microscopy of phage suspensions for personalised phage therapy.
Uloženo v:
| Název: | Real-time monitoring by interferometric light microscopy of phage suspensions for personalised phage therapy. |
|---|---|
| Autoři: | Lapras, Benjamine, Merienne, Camille, Eynaud, Emma, Usseglio, Léa, Marchand, Chloé, Médina, Mathieu, Kolenda, Camille, Briot, Thomas, Laurent, Frédéric, Pirot, Fabrice, PHAGEinLYON |
| Zdroj: | Scientific Reports; 12/30/2024, Vol. 14 Issue 1, p1-15, 15p |
| Témata: | MICROSCOPY, LIGHT scattering, DRUG resistance in bacteria, QUALITY control, PROOF of concept |
| Abstrakt: | Phage therapy uses viruses (phages) against antibiotic resistance. Tailoring treatments to specific patient strains requires stocks of various highly concentrated purified phages. It, therefore, faces challenges: titration duration and specificity to a phage/bacteria couple; purification affecting stability; and highly concentrated suspensions tending to aggregate. To address these challenges, interferometric light microscopy (ILM), characterising particles (size, concentration, and visual homogeneity) within minutes, was applied herein to anti-Staphylococcus aureus myovirus phage suspensions. Particle concentration was linearly correlated with phage infectious titre (R2 > 0.97, slope: 3 particles/plaque forming units (PFU)) at various degrees of purification, allowing to approximate the infectious titre for suspensions ≥ 3 × 108 PFU/mL, thereby encompassing most therapeutic doses. Purification narrowed and homogenised particle distribution while maintaining therapeutic concentrations. When compared to dynamic light scattering, electrophoretic mobility, and UV/Visible-spectroscopy, ILM best detected aggregates according to our homemade scoring. Although ILM has certain limitations, such as the inability to detect podoviruses (hydrodynamic diameter < 80 nm), or to measure particles in low-concentrated suspensions (< 108 particles/mL), the present proof-of-concept positions this technique as a valuable quality control tool, as a complement to titration rather than a replacement for this technique, for phage suspensions, paving the way for further investigations. [ABSTRACT FROM AUTHOR] |
| Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Databáze: | Complementary Index |
Full Text 
Full Text Finder 
Nájsť tento článok vo Web of Science Buďte první, kdo okomentuje tento záznam!