Effect of endophytic diazotroph Enterobacter roggenkampii ED5 on nitrogen-metabolism-related microecology in the sugarcane rhizosphere at different nitrogen levels.
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| Název: | Effect of endophytic diazotroph Enterobacter roggenkampii ED5 on nitrogen-metabolism-related microecology in the sugarcane rhizosphere at different nitrogen levels. |
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| Autoři: | Dao-Jun Guo, Dong-Ping Li, Bin Yang, Verma, Krishan K., Singh, Rajesh Kumar, Singh, Pratiksha, Khan, Qaisar, Sharma, Anjney, Ying Qin, Bao-Qing Zhang, Xiu-Peng Song, Yang-Rui Li |
| Zdroj: | Frontiers in Microbiology; 2023, p1-15, 15p |
| Témata: | SUGARCANE, NITROGEN fertilizers, RHIZOSPHERE, MICROBIAL ecology, ENERGY crops, SUGAR crops |
| Abstrakt: | Sugarcane is an important sugar and energy crop worldwide, requiring a large amount of nitrogen (N). However, excessive application of synthetic N fertilizer causes environmental pollution in farmland. Endophytic nitrogen-fixing bacteria (ENFB) provide N nutrition for plants through biological N fixation, thus reducing the need for chemical fertilizers. The present study investigated the effect of the N-fixing endophytic strain Enterobacter roggenkampii ED5 on phytohormone indole-3-acetic acid (IAA), N-metabolism enzyme activities, microbial community compositions, and N cycle genes in sugarcane rhizosphere soil at different N levels. Three levels of 15N-urea, such as low N (0 kg/ha), medium N (150 kg/ha), and high N (300 kg/ha), were applied. The results showed that, after inoculating strain ED5, the IAA content in sugarcane leaves was significantly increased by 68.82% under lowN condition at the seedling stage (60 days). The nitrate reductase (NR) activity showed a downward trend. However, the glutamine synthase (GS) and NADH-glutamate dehydrogenase (NADH-GDH) activities were significantly enhanced compared to the control under the high N condition, and the GS and NR genes had the highest expression at 180 and 120 days, respectively, at the low N level. The total N content in the roots, stems, and leaves of sugarcane was higher than the control. The 15N atom % excess of sugarcane decreased significantly under medium N condition, indicating that the medium N level was conducive to N fixation in strain ED5. Metagenome analysis of sugarcane rhizosphere soil exhibited that the abundance of N-metabolizing microbial richness was increased under low and high N conditions after inoculation of strain ED5 at the genus level, while it was increased at the phylum level only under the low N condition. The LefSe (LDA > 2, p < 0.05) found that the N-metabolism-related differential microorganisms under the highNcondition were higher than those undermedium and low N conditions. It was also shown that the abundance of nifDHK genes was significantly increased after inoculation of ED5 at the medium N level, and other N cycle genes had high abundance at the high N level after inoculation of strain ED5. The results of this study provided a scientific reference for N fertilization in actual sugarcane production. [ABSTRACT FROM AUTHOR] |
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| Databáze: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Effect of endophytic diazotroph Enterobacter roggenkampii ED5 on nitrogen-metabolism-related microecology in the sugarcane rhizosphere at different nitrogen levels. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dao-Jun+Guo%22">Dao-Jun Guo</searchLink><br /><searchLink fieldCode="AR" term="%22Dong-Ping+Li%22">Dong-Ping Li</searchLink><br /><searchLink fieldCode="AR" term="%22Bin+Yang%22">Bin Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Verma%2C+Krishan+K%2E%22">Verma, Krishan K.</searchLink><br /><searchLink fieldCode="AR" term="%22Singh%2C+Rajesh+Kumar%22">Singh, Rajesh Kumar</searchLink><br /><searchLink fieldCode="AR" term="%22Singh%2C+Pratiksha%22">Singh, Pratiksha</searchLink><br /><searchLink fieldCode="AR" term="%22Khan%2C+Qaisar%22">Khan, Qaisar</searchLink><br /><searchLink fieldCode="AR" term="%22Sharma%2C+Anjney%22">Sharma, Anjney</searchLink><br /><searchLink fieldCode="AR" term="%22Ying+Qin%22">Ying Qin</searchLink><br /><searchLink fieldCode="AR" term="%22Bao-Qing+Zhang%22">Bao-Qing Zhang</searchLink><br /><searchLink fieldCode="AR" term="%22Xiu-Peng+Song%22">Xiu-Peng Song</searchLink><br /><searchLink fieldCode="AR" term="%22Yang-Rui+Li%22">Yang-Rui Li</searchLink> – Name: TitleSource Label: Source Group: Src Data: Frontiers in Microbiology; 2023, p1-15, 15p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22SUGARCANE%22">SUGARCANE</searchLink><br /><searchLink fieldCode="DE" term="%22NITROGEN+fertilizers%22">NITROGEN fertilizers</searchLink><br /><searchLink fieldCode="DE" term="%22RHIZOSPHERE%22">RHIZOSPHERE</searchLink><br /><searchLink fieldCode="DE" term="%22MICROBIAL+ecology%22">MICROBIAL ecology</searchLink><br /><searchLink fieldCode="DE" term="%22ENERGY+crops%22">ENERGY crops</searchLink><br /><searchLink fieldCode="DE" term="%22SUGAR+crops%22">SUGAR crops</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Sugarcane is an important sugar and energy crop worldwide, requiring a large amount of nitrogen (N). However, excessive application of synthetic N fertilizer causes environmental pollution in farmland. Endophytic nitrogen-fixing bacteria (ENFB) provide N nutrition for plants through biological N fixation, thus reducing the need for chemical fertilizers. The present study investigated the effect of the N-fixing endophytic strain Enterobacter roggenkampii ED5 on phytohormone indole-3-acetic acid (IAA), N-metabolism enzyme activities, microbial community compositions, and N cycle genes in sugarcane rhizosphere soil at different N levels. Three levels of 15N-urea, such as low N (0 kg/ha), medium N (150 kg/ha), and high N (300 kg/ha), were applied. The results showed that, after inoculating strain ED5, the IAA content in sugarcane leaves was significantly increased by 68.82% under lowN condition at the seedling stage (60 days). The nitrate reductase (NR) activity showed a downward trend. However, the glutamine synthase (GS) and NADH-glutamate dehydrogenase (NADH-GDH) activities were significantly enhanced compared to the control under the high N condition, and the GS and NR genes had the highest expression at 180 and 120 days, respectively, at the low N level. The total N content in the roots, stems, and leaves of sugarcane was higher than the control. The 15N atom % excess of sugarcane decreased significantly under medium N condition, indicating that the medium N level was conducive to N fixation in strain ED5. Metagenome analysis of sugarcane rhizosphere soil exhibited that the abundance of N-metabolizing microbial richness was increased under low and high N conditions after inoculation of strain ED5 at the genus level, while it was increased at the phylum level only under the low N condition. The LefSe (LDA > 2, p < 0.05) found that the N-metabolism-related differential microorganisms under the highNcondition were higher than those undermedium and low N conditions. It was also shown that the abundance of nifDHK genes was significantly increased after inoculation of ED5 at the medium N level, and other N cycle genes had high abundance at the high N level after inoculation of strain ED5. The results of this study provided a scientific reference for N fertilization in actual sugarcane production. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Frontiers in Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3389/fmicb.2023.1132016 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 1 Subjects: – SubjectFull: SUGARCANE Type: general – SubjectFull: NITROGEN fertilizers Type: general – SubjectFull: RHIZOSPHERE Type: general – SubjectFull: MICROBIAL ecology Type: general – SubjectFull: ENERGY crops Type: general – SubjectFull: SUGAR crops Type: general Titles: – TitleFull: Effect of endophytic diazotroph Enterobacter roggenkampii ED5 on nitrogen-metabolism-related microecology in the sugarcane rhizosphere at different nitrogen levels. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dao-Jun Guo – PersonEntity: Name: NameFull: Dong-Ping Li – PersonEntity: Name: NameFull: Bin Yang – PersonEntity: Name: NameFull: Verma, Krishan K. – PersonEntity: Name: NameFull: Singh, Rajesh Kumar – PersonEntity: Name: NameFull: Singh, Pratiksha – PersonEntity: Name: NameFull: Khan, Qaisar – PersonEntity: Name: NameFull: Sharma, Anjney – PersonEntity: Name: NameFull: Ying Qin – PersonEntity: Name: NameFull: Bao-Qing Zhang – PersonEntity: Name: NameFull: Xiu-Peng Song – PersonEntity: Name: NameFull: Yang-Rui Li IsPartOfRelationships: – BibEntity: Dates: – D: 11 M: 10 Text: 2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 1664302X Titles: – TitleFull: Frontiers in Microbiology Type: main |
| ResultId | 1 |