Genome analysis of a halophilic Virgibacillus halodenitrificans ASH15 revealed salt adaptation, plant growth promotion, and isoprenoid biosynthetic machinery.
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| Název: | Genome analysis of a halophilic Virgibacillus halodenitrificans ASH15 revealed salt adaptation, plant growth promotion, and isoprenoid biosynthetic machinery. |
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| Autoři: | Sharma, Anjney, Singh, Ram Nageena, Xiu-Peng Song, Singh, Rajesh Kumar, Dao-Jun Guo, Singh, Pratiksha, Verma, Krishan K., Yang-Rui Li |
| Zdroj: | Frontiers in Microbiology; 2023, p01-25, 25p |
| Témata: | PLANT growth, HORIZONTAL gene transfer, BETAINE, ISOPENTENOIDS, GENOMES, NITROGEN fixation, PLANT colonization |
| Abstrakt: | Globally, due to widespread dispersion, intraspecific diversity, and crucial ecological components of halophilic ecosystems, halophilic bacteria is considered one of the key models for ecological, adaptative, and biotechnological applications research in saline environments. With this aim, the present study was to enlighten the plant growth-promoting features and investigate the systematic genome of a halophilic bacteria, Virgibacillus halodenitrificans ASH15, through single-molecule real-time (SMRT) sequencing technology. Results showed that strain ASH15 could survive in high salinity up to 25% (w/v) NaCl concentration and express plant growth-promoting traits such as nitrogen fixation, plant growth hormones, and hydrolytic enzymes, which sustain salt stress. The results of pot experiment revealed that strain ASH15 significantly enhanced sugarcane plant growth (root shoot length and weight) under salt stress conditions. Moreover, the sequencing analysis of the strain ASH15 genome exhibited that this strain contained a circular chromosome of 3,832,903 bp with an average G+C content of 37.54%: 3721 predicted protein-coding sequences (CDSs), 24 rRNA genes, and 62 tRNA genes. Genome analysis revealed that the genes related to the synthesis and transport of compatible solutes (glycine, betaine, ectoine, hydroxyectoine, and glutamate) confirm salt stress as well as heavy metal resistance. Furthermore, functional annotation showed that the strain ASH15 encodes genes for root colonization, biofilm formation, phytohormone IAA production, nitrogen fixation, phosphate metabolism, and siderophore production, which are beneficial for plant growth promotion. Strain ASH15 also has a gene resistance to antibiotics and pathogens. In addition, analysis also revealed that the genome strain ASH15 has insertion sequences and CRISPRs, which suggest its ability to acquire new genes through horizontal gene transfer and acquire immunity to the attack of viruses. This work provides knowledge of the mechanism through which V. halodenitrificans ASH15 tolerates salt stress. Deep genome analysis, identified MVA pathway involved in biosynthesis of isoprenoids, more precisely "Squalene." Squalene has various applications, such as an antioxidant, anti-cancer agent, anti-aging agent, hemopreventive agent, anti-bacterial agent, adjuvant for vaccines and drug carriers, and detoxifier.Our findings indicated that strain ASH15 has enormous potential in industries such as in agriculture, pharmaceuticals, cosmetics, and food. [ABSTRACT FROM AUTHOR] |
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| Databáze: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Genome analysis of a halophilic Virgibacillus halodenitrificans ASH15 revealed salt adaptation, plant growth promotion, and isoprenoid biosynthetic machinery. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Sharma%2C+Anjney%22">Sharma, Anjney</searchLink><br /><searchLink fieldCode="AR" term="%22Singh%2C+Ram+Nageena%22">Singh, Ram Nageena</searchLink><br /><searchLink fieldCode="AR" term="%22Xiu-Peng+Song%22">Xiu-Peng Song</searchLink><br /><searchLink fieldCode="AR" term="%22Singh%2C+Rajesh+Kumar%22">Singh, Rajesh Kumar</searchLink><br /><searchLink fieldCode="AR" term="%22Dao-Jun+Guo%22">Dao-Jun Guo</searchLink><br /><searchLink fieldCode="AR" term="%22Singh%2C+Pratiksha%22">Singh, Pratiksha</searchLink><br /><searchLink fieldCode="AR" term="%22Verma%2C+Krishan+K%2E%22">Verma, Krishan K.</searchLink><br /><searchLink fieldCode="AR" term="%22Yang-Rui+Li%22">Yang-Rui Li</searchLink> – Name: TitleSource Label: Source Group: Src Data: Frontiers in Microbiology; 2023, p01-25, 25p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22PLANT+growth%22">PLANT growth</searchLink><br /><searchLink fieldCode="DE" term="%22HORIZONTAL+gene+transfer%22">HORIZONTAL gene transfer</searchLink><br /><searchLink fieldCode="DE" term="%22BETAINE%22">BETAINE</searchLink><br /><searchLink fieldCode="DE" term="%22ISOPENTENOIDS%22">ISOPENTENOIDS</searchLink><br /><searchLink fieldCode="DE" term="%22GENOMES%22">GENOMES</searchLink><br /><searchLink fieldCode="DE" term="%22NITROGEN+fixation%22">NITROGEN fixation</searchLink><br /><searchLink fieldCode="DE" term="%22PLANT+colonization%22">PLANT colonization</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Globally, due to widespread dispersion, intraspecific diversity, and crucial ecological components of halophilic ecosystems, halophilic bacteria is considered one of the key models for ecological, adaptative, and biotechnological applications research in saline environments. With this aim, the present study was to enlighten the plant growth-promoting features and investigate the systematic genome of a halophilic bacteria, Virgibacillus halodenitrificans ASH15, through single-molecule real-time (SMRT) sequencing technology. Results showed that strain ASH15 could survive in high salinity up to 25% (w/v) NaCl concentration and express plant growth-promoting traits such as nitrogen fixation, plant growth hormones, and hydrolytic enzymes, which sustain salt stress. The results of pot experiment revealed that strain ASH15 significantly enhanced sugarcane plant growth (root shoot length and weight) under salt stress conditions. Moreover, the sequencing analysis of the strain ASH15 genome exhibited that this strain contained a circular chromosome of 3,832,903 bp with an average G+C content of 37.54%: 3721 predicted protein-coding sequences (CDSs), 24 rRNA genes, and 62 tRNA genes. Genome analysis revealed that the genes related to the synthesis and transport of compatible solutes (glycine, betaine, ectoine, hydroxyectoine, and glutamate) confirm salt stress as well as heavy metal resistance. Furthermore, functional annotation showed that the strain ASH15 encodes genes for root colonization, biofilm formation, phytohormone IAA production, nitrogen fixation, phosphate metabolism, and siderophore production, which are beneficial for plant growth promotion. Strain ASH15 also has a gene resistance to antibiotics and pathogens. In addition, analysis also revealed that the genome strain ASH15 has insertion sequences and CRISPRs, which suggest its ability to acquire new genes through horizontal gene transfer and acquire immunity to the attack of viruses. This work provides knowledge of the mechanism through which V. halodenitrificans ASH15 tolerates salt stress. Deep genome analysis, identified MVA pathway involved in biosynthesis of isoprenoids, more precisely "Squalene." Squalene has various applications, such as an antioxidant, anti-cancer agent, anti-aging agent, hemopreventive agent, anti-bacterial agent, adjuvant for vaccines and drug carriers, and detoxifier.Our findings indicated that strain ASH15 has enormous potential in industries such as in agriculture, pharmaceuticals, cosmetics, and food. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Frontiers in Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3389/fmicb.2023.1229955 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 25 StartPage: 01 Subjects: – SubjectFull: PLANT growth Type: general – SubjectFull: HORIZONTAL gene transfer Type: general – SubjectFull: BETAINE Type: general – SubjectFull: ISOPENTENOIDS Type: general – SubjectFull: GENOMES Type: general – SubjectFull: NITROGEN fixation Type: general – SubjectFull: PLANT colonization Type: general Titles: – TitleFull: Genome analysis of a halophilic Virgibacillus halodenitrificans ASH15 revealed salt adaptation, plant growth promotion, and isoprenoid biosynthetic machinery. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Sharma, Anjney – PersonEntity: Name: NameFull: Singh, Ram Nageena – PersonEntity: Name: NameFull: Xiu-Peng Song – PersonEntity: Name: NameFull: Singh, Rajesh Kumar – PersonEntity: Name: NameFull: Dao-Jun Guo – PersonEntity: Name: NameFull: Singh, Pratiksha – PersonEntity: Name: NameFull: Verma, Krishan K. – PersonEntity: Name: NameFull: Yang-Rui Li IsPartOfRelationships: – BibEntity: Dates: – D: 06 M: 10 Text: 2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 1664302X Titles: – TitleFull: Frontiers in Microbiology Type: main |
| ResultId | 1 |