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A sustainable strategy for the assembly of Glypromate® and its structurally-related analogues by tandem sequential peptide coupling.

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Název: A sustainable strategy for the assembly of Glypromate® and its structurally-related analogues by tandem sequential peptide coupling.
Autoři: Sampaio-Dias, Ivo E., Pinto da Silva, Luís, Silva, Sandra G., García-Mera, Xerardo, Rodríguez-Borges, José E.
Zdroj: Green Chemistry; 6/7/2020, Vol. 22 Issue 11, p3584-3596, 13p
Témata: PEPTIDE bonds, ALZHEIMER'S disease, AMINO acids
Abstrakt: This work describes an improved and greener methodology of solution-phase synthesis for the preparation of Glypromate® (glycyl- L -prolyl- L -glutamic acid, GPE), a potent neuropeptide for applications in neurodegenerative conditions such as Huntington's, Parkinson's and Alzheimer's diseases. This protocol comprises the assembly of the perbenzylated form of Glypromate® [Cbz-Gly-Pro-Glu(OBn)-OBn (5)] from L -proline. Following a tandem sequential peptide coupling strategy, two chemoselective peptide bonds are formed without the need for purifying the intermediates ensuing a one-pot fashion synthesis. EcoScale score and E-factor were selected as the green metrics to assess the environmental impact of the preparation of tripeptide 5 using this protocol. After optimization and application of greener conditions, intermediate 5 was obtained with 95% global yield and 99% purity (NMR, HRMS, and rp-HPLC), with excellent final EcoScale score of 75 out of 100 and global E-factor of 1.8. Glypromate® is achieved by removing N- and C-protecting groups by hydrogenolysis using Pd/C as the catalyst in 98% yield, avoiding chromatographic techniques. Moreover, the protocol ensures stereochemical integrity (determined by VT-NMR and rp-HPLC) and was also successfully applied for the preparation of structurally-related Glypromate® analogues with higher degree of molecular complexity compatible with functionalized amino acids with different side chains. For the first time a one-pot protocol for the assembly of tripeptides with the removal of protecting groups in the same reaction vessel is reported. [ABSTRACT FROM AUTHOR]
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Databáze: Complementary Index
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Abstrakt:This work describes an improved and greener methodology of solution-phase synthesis for the preparation of Glypromate® (glycyl- L -prolyl- L -glutamic acid, GPE), a potent neuropeptide for applications in neurodegenerative conditions such as Huntington's, Parkinson's and Alzheimer's diseases. This protocol comprises the assembly of the perbenzylated form of Glypromate® [Cbz-Gly-Pro-Glu(OBn)-OBn (5)] from L -proline. Following a tandem sequential peptide coupling strategy, two chemoselective peptide bonds are formed without the need for purifying the intermediates ensuing a one-pot fashion synthesis. EcoScale score and E-factor were selected as the green metrics to assess the environmental impact of the preparation of tripeptide 5 using this protocol. After optimization and application of greener conditions, intermediate 5 was obtained with 95% global yield and 99% purity (NMR, HRMS, and rp-HPLC), with excellent final EcoScale score of 75 out of 100 and global E-factor of 1.8. Glypromate® is achieved by removing N- and C-protecting groups by hydrogenolysis using Pd/C as the catalyst in 98% yield, avoiding chromatographic techniques. Moreover, the protocol ensures stereochemical integrity (determined by VT-NMR and rp-HPLC) and was also successfully applied for the preparation of structurally-related Glypromate® analogues with higher degree of molecular complexity compatible with functionalized amino acids with different side chains. For the first time a one-pot protocol for the assembly of tripeptides with the removal of protecting groups in the same reaction vessel is reported. [ABSTRACT FROM AUTHOR]
ISSN:14639262
DOI:10.1039/d0gc00143k