Core microRNAs regulate neural crest delamination and condensation in the developing trigeminal ganglion.
Uložené v:
| Názov: | Core microRNAs regulate neural crest delamination and condensation in the developing trigeminal ganglion. |
|---|---|
| Autori: | Marquez RB; Laboratory of Developmental Biology, Instituto Tecnológico de Chascomús, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (CONICET-UNSAM), Chascomús 7130, Argentina.; Escuela de Bio y Nanotecnologías (UNSAM), Chascomús 7130, Argentina., Sánchez Vásquez E; Laboratory of Developmental Biology, Instituto Tecnológico de Chascomús, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (CONICET-UNSAM), Chascomús 7130, Argentina.; Escuela de Bio y Nanotecnologías (UNSAM), Chascomús 7130, Argentina., Alonso AM; Escuela de Bio y Nanotecnologías (UNSAM), Chascomús 7130, Argentina.; Laboratorio de Parasitología Molecular, Instituto Tecnológico Chascomús, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (CONICET-UNSAM), Chascomús 7130, Argentina., Bernardi YE; Laboratory of Developmental Biology, Instituto Tecnológico de Chascomús, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (CONICET-UNSAM), Chascomús 7130, Argentina.; Escuela de Bio y Nanotecnologías (UNSAM), Chascomús 7130, Argentina., Santillan EM; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205., Lwigale P; Biochemistry and Cell Biology Department, Rice University, Houston, TX 77251-1892., Cochella L; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205., Bronner ME; Division of Biology, California Institute of Technology, Pasadena, CA 91125., Strobl-Mazzulla PH; Laboratory of Developmental Biology, Instituto Tecnológico de Chascomús, Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (CONICET-UNSAM), Chascomús 7130, Argentina.; Escuela de Bio y Nanotecnologías (UNSAM), Chascomús 7130, Argentina. |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2025 Dec 09; Vol. 122 (49), pp. e2517668122. Date of Electronic Publication: 2025 Dec 02. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : National Academy of Sciences |
| Výrazy zo slovníka MeSH: | Neural Crest*/embryology , Neural Crest*/cytology , Neural Crest*/metabolism , MicroRNAs*/genetics , MicroRNAs*/metabolism , Trigeminal Ganglion*/embryology , Trigeminal Ganglion*/cytology , Trigeminal Ganglion*/metabolism, Animals ; Chick Embryo ; Cell Differentiation ; Gene Expression Regulation, Developmental ; Epithelial-Mesenchymal Transition/genetics ; Cell Movement |
| Abstrakt: | Competing Interests: Competing interests statement:The authors declare no competing interest. Cranial neural crest cells (NCCs) undergo dynamic processes during embryonic development, including delamination from the neural tube by epithelial-to-mesenchymal transition (EMT), migration to the periphery, condensation via mesenchymal-to-epithelial transition (MET), and differentiation into structures like the trigeminal ganglion. Here, we identify and characterize the function of a core set of miRNAs involved in these transitions during the formation of the trigeminal ganglion in the chick embryo. We further identify putative targets of miRNAs involved in neural crest EMT and MET. Notably, introducing MET-involved miRNAs into trunk NCCs endows these cells with the ability to condense and differentiate into neurons in vivo in a manner reminiscent of cranial rather than trunk NCCs. Our findings shed light on the intricate regulatory networks governing NCC behavior, positioning miRNAs as key regulatory elements required for migratory transitions and axial level-specific differentiation capabilities. |
| Grant Information: | 304976_Z _ 24 _ Z Wellcome Trust (WT) |
| Contributed Indexing: | Keywords: condensation; microRNAs; migration; neural crest; trigeminal ganglion |
| Substance Nomenclature: | 0 (MicroRNAs) |
| Entry Date(s): | Date Created: 20251202 Date Completed: 20251202 Latest Revision: 20251202 |
| Update Code: | 20251203 |
| DOI: | 10.1073/pnas.2517668122 |
| PMID: | 41329730 |
| Databáza: | MEDLINE |
Nájsť tento článok vo Web of Science | Abstrakt: | Competing Interests: Competing interests statement:The authors declare no competing interest.<br />Cranial neural crest cells (NCCs) undergo dynamic processes during embryonic development, including delamination from the neural tube by epithelial-to-mesenchymal transition (EMT), migration to the periphery, condensation via mesenchymal-to-epithelial transition (MET), and differentiation into structures like the trigeminal ganglion. Here, we identify and characterize the function of a core set of miRNAs involved in these transitions during the formation of the trigeminal ganglion in the chick embryo. We further identify putative targets of miRNAs involved in neural crest EMT and MET. Notably, introducing MET-involved miRNAs into trunk NCCs endows these cells with the ability to condense and differentiate into neurons in vivo in a manner reminiscent of cranial rather than trunk NCCs. Our findings shed light on the intricate regulatory networks governing NCC behavior, positioning miRNAs as key regulatory elements required for migratory transitions and axial level-specific differentiation capabilities. |
|---|---|
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.2517668122 |