Injectable chitosan-based thermosensitive hydrogel loaded with adipose-derived mesenchymal stem cells promotes pressure ulcer healing.
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| Názov: | Injectable chitosan-based thermosensitive hydrogel loaded with adipose-derived mesenchymal stem cells promotes pressure ulcer healing. |
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| Autori: | He S; College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China., Lin B; Fujian Provincial Center for Drug Evaluation and Monitoring, No. 156 Dongpu Road, Fuzhou 350003, China., Zhang C; College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China., He S; College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China., Zheng Y; Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China., Shi X; College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China; Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China. Electronic address: shixa@fzu.edu.cn., Yang J; College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China; Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China. Electronic address: jmyang@fzu.edu.cn. |
| Zdroj: | Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2025 Dec; Vol. 256 (Pt 2), pp. 115089. Date of Electronic Publication: 2025 Aug 29. |
| Spôsob vydávania: | Journal Article |
| Jazyk: | English |
| Informácie o časopise: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| Výrazy zo slovníka MeSH: | Chitosan*/chemistry , Chitosan*/pharmacology , Pressure Ulcer*/therapy , Pressure Ulcer*/pathology , Mesenchymal Stem Cells*/cytology , Mesenchymal Stem Cells*/drug effects , Hydrogels*/chemistry , Hydrogels*/pharmacology , Wound Healing*/drug effects , Adipose Tissue*/cytology , Mesenchymal Stem Cell Transplantation*, Animals ; Rats ; Male ; Rats, Sprague-Dawley ; Temperature ; Glycerophosphates/chemistry ; Injections ; Cell Proliferation/drug effects ; Gelatin/chemistry ; Cells, Cultured ; Cell Survival/drug effects |
| Abstrakt: | Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Pressure ulcers, resulting from prolonged external pressure or shear forces on skin and underlying tissues over bony prominences, lead to tissue ischemia and impaired lymphatic drainage. Without timely intervention, these wounds can progress to severe complications including cellulitis, chronic infections, and osteomyelitis. In this study, we developed a chitosan/sodium β-glycerophosphate/gelatin (CS/β-GP/GEL) thermosensitive hydrogel system to enhance the therapeutic efficacy of adipose-derived mesenchymal stem cells (ADSCs) in pressure ulcer healing. The incorporation of gelatin addresses the limitations of conventional CS/β-GP hydrogels, such as low mechanical strength and poor biocompatibility. The optimized CS/β-GP/GEL hydrogel exhibits good injectability, suitable gel formation time and pH, facilitating efficient ADSCs encapsulation. Furthermore, the hydrogel demonstrates good water absorption capacity, degradability, and rheological properties, making it suitable for biomedical applications. In vitro studies confirmed the hydrogel's high cytocompatibility, supporting ADSCs viability and proliferation. Additionally, the CS/β-GP/GEL@ADSC composite demonstrated pro-angiogenic properties, suppressed reactive oxygen species-mediated apoptosis, and facilitated the accumulation of M2-type macrophages. In vivo evaluations revealed that the CS/β-GP/GEL@ADSC composite had high histocompatibility and accelerated pressure ulcer healing in a rat model, mediated through enhanced angiogenesis, M2-dominant macrophage polarization, and improved extracellular matrix remodeling. These findings highlight the potential of CS/β-GP/GEL@ADSC as a promising therapeutic strategy for pressure ulcer management. (Copyright © 2025 Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: Chitosan; Mesenchymal stem cell; Pressure ulcer; Thermosensitive hydrogel; Wound healing |
| Substance Nomenclature: | 9012-76-4 (Chitosan) 0 (Hydrogels) 0 (Glycerophosphates) 9000-70-8 (Gelatin) WWH06G87W6 (beta-glycerophosphoric acid) |
| Entry Date(s): | Date Created: 20250901 Date Completed: 20250909 Latest Revision: 20250909 |
| Update Code: | 20250910 |
| DOI: | 10.1016/j.colsurfb.2025.115089 |
| PMID: | 40889476 |
| Databáza: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br />Pressure ulcers, resulting from prolonged external pressure or shear forces on skin and underlying tissues over bony prominences, lead to tissue ischemia and impaired lymphatic drainage. Without timely intervention, these wounds can progress to severe complications including cellulitis, chronic infections, and osteomyelitis. In this study, we developed a chitosan/sodium β-glycerophosphate/gelatin (CS/β-GP/GEL) thermosensitive hydrogel system to enhance the therapeutic efficacy of adipose-derived mesenchymal stem cells (ADSCs) in pressure ulcer healing. The incorporation of gelatin addresses the limitations of conventional CS/β-GP hydrogels, such as low mechanical strength and poor biocompatibility. The optimized CS/β-GP/GEL hydrogel exhibits good injectability, suitable gel formation time and pH, facilitating efficient ADSCs encapsulation. Furthermore, the hydrogel demonstrates good water absorption capacity, degradability, and rheological properties, making it suitable for biomedical applications. In vitro studies confirmed the hydrogel's high cytocompatibility, supporting ADSCs viability and proliferation. Additionally, the CS/β-GP/GEL@ADSC composite demonstrated pro-angiogenic properties, suppressed reactive oxygen species-mediated apoptosis, and facilitated the accumulation of M2-type macrophages. In vivo evaluations revealed that the CS/β-GP/GEL@ADSC composite had high histocompatibility and accelerated pressure ulcer healing in a rat model, mediated through enhanced angiogenesis, M2-dominant macrophage polarization, and improved extracellular matrix remodeling. These findings highlight the potential of CS/β-GP/GEL@ADSC as a promising therapeutic strategy for pressure ulcer management.<br /> (Copyright © 2025 Elsevier B.V. All rights reserved.) |
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| ISSN: | 1873-4367 |
| DOI: | 10.1016/j.colsurfb.2025.115089 |