Cytokine Antibody Microarray-Based Proteomic Strategies for Characterizing the Dysregulation of Cytokines Disease-Specific.
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| Název: | Cytokine Antibody Microarray-Based Proteomic Strategies for Characterizing the Dysregulation of Cytokines Disease-Specific. |
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| Autoři: | Barderas R; Functional Proteomics Unit, Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Madrid, Spain. r.barderasm@isciii.es.; CIBER of Frailty and Healthy Aging (CIBERFES), Madrid, Spain. r.barderasm@isciii.es., San Segundo-Acosta P; Functional Proteomics Unit, Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Madrid, Spain., Montero-Calle A; Functional Proteomics Unit, Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Madrid, Spain. |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2929, pp. 195-213. |
| Způsob vydávání: | Journal Article |
| Jazyk: | English |
| Informace o časopise: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| Výrazy ze slovníku MeSH: | Proteomics*/methods , Protein Array Analysis*/methods , Cytokines*/metabolism , Cytokines*/immunology , Antibodies*/immunology, Humans ; Biomarkers |
| Abstrakt: | The understanding of disease mechanisms and the search for potential therapeutic targets have driven the focus of proteomics studies towards the identification of specific protein profiles and molecular pathways disease associated. Protein microarrays offer high-throughput alternatives to mass spectrometry, with peptide, phage, and recombinant protein microarrays providing different options for studying disease-specific alterations. Commercially available antibody microarrays, particularly cytokine arrays, enable the simultaneous analysis of multiple cytokine expression levels, offering valuable insights into disease processes, drug efficacy, and biomarker discovery. Indeed, cytokine arrays outperform mass spectrometry methods due to their ability to analyze cytokines at an extremely low concentration in parallel in a high-throughput manner. Here, we outline protocols for cytokine analysis using cytokine antibody microarrays, detailing sandwich approaches and direct or indirect incubation methods for the characterization of disease-associated cytokine profiles. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| Contributed Indexing: | Keywords: Antibody microarray; Cytokine array; Cytokines; Growth factors; Proteomics immunomodulators; Screening |
| Substance Nomenclature: | 0 (Cytokines) 0 (Antibodies) 0 (Biomarkers) |
| Entry Date(s): | Date Created: 20250702 Date Completed: 20250702 Latest Revision: 20250702 |
| Update Code: | 20250702 |
| DOI: | 10.1007/978-1-0716-4595-6_15 |
| PMID: | 40601153 |
| Databáze: | MEDLINE |
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