Parallel assembly of dual-electrochemical cell: a novel approach for simultaneous multiplexed sensing analysis.
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| Title: | Parallel assembly of dual-electrochemical cell: a novel approach for simultaneous multiplexed sensing analysis. |
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| Authors: | Romanholo PVV; Instituto de Química, Universidade Federal de Goiás, Goiânia, 74690-900, GO, Brazil., Andrade LM; Instituto de Química, Universidade Federal de Goiás, Goiânia, 74690-900, GO, Brazil., Giglioti M; Metrohm Brasil Instrumentação Analítica Ltda, São Paulo, 05007-030, SP, Brazil., Luccas GZA; Metrohm Brasil Instrumentação Analítica Ltda, São Paulo, 05007-030, SP, Brazil., Machado SAS; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, 13566-590, SP, Brazil., Sgobbi LF; Instituto de Química, Universidade Federal de Goiás, Goiânia, 74690-900, GO, Brazil. livia_sgobbi@ufg.br. |
| Source: | Mikrochimica acta [Mikrochim Acta] 2025 May 07; Vol. 192 (6), pp. 340. Date of Electronic Publication: 2025 May 07. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Wien ; New York : Springer-Verlag. |
| MeSH Terms: | Electrochemical Techniques*/instrumentation , Electrochemical Techniques*/methods , Ascorbic Acid*/analysis , Biosensing Techniques*/methods , Biosensing Techniques*/instrumentation, Limit of Detection ; Electrodes |
| Abstract: | In the field of biosensing and chemical sensing, there is a growing demand for multiplexed detection and quantification of multiple targets within complex matrices. In electrochemical sensing, simultaneous multiplexed analysis is typically performed with multiple electrodes connected to a multichannel potentiostat. An alternative strategy involves using a single electrode capable of discriminating and detecting several analytes in a single measurement, which is, however, unfortunately limited to a selective group of molecules. Herein, we report a novel electrochemical method based on the parallel assembly of a dual-electrochemical cell (PADEC), which enables the simultaneous detection and quantification of solvent-incompatible analytes, prepared separately in two distinct electrochemical cells, using a single-channel potentiostat-thus achieving multichannel-like performance. This approach relies on connecting two electrochemical cells in parallel, allowing the concurrent measurement of distinct electrochemical responses from analytes that otherwise could not be simultaneously determined due to solvent incompatibility. As a proof of concept, the water-soluble vitamin C, and the lipid-soluble vitamin D3 were simultaneously determined, each in its respective optimized medium. The PADEC approach demonstrated performance comparable to individual detection methods, achieving limits of detection of 27 μM for vitamin C and 32 μM for vitamin D3 over a linear range of 20-400 μM. This strategy establishes a new approach for simultaneous, multiplexed electrochemical determination of analytes in different media. Moreover, this innovation may extend applications in electrochemistry beyond (bio)sensing to include areas such as electrocatalysis, energy and corrosion, potentially reducing dependence on multichannel potentiostats. (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.) |
| Competing Interests: | Declarations. Conflict of interest: The authors have no conflicts of interest to declare that are relevant to the content of this article. |
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| Grant Information: | 88882.386428/2019-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 403306/2023-0 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 202310267001369 Fundação de Amparo à Pesquisa do Estado de Goiás |
| Contributed Indexing: | Keywords: Electrochemical sensors; Monochannel potentiostat; Multiplexed analysis; Simultaneous detection; Vitamin C; Vitamin D3 |
| Substance Nomenclature: | PQ6CK8PD0R (Ascorbic Acid) |
| Entry Date(s): | Date Created: 20250506 Date Completed: 20250507 Latest Revision: 20250610 |
| Update Code: | 20250611 |
| DOI: | 10.1007/s00604-025-07194-x |
| PMID: | 40328952 |
| Database: | MEDLINE |
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