Gambierdiscus polynesiensis from New Caledonia (South West Pacific Ocean): Morpho-molecular characterization, toxin profile and response to light intensity.
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| Title: | Gambierdiscus polynesiensis from New Caledonia (South West Pacific Ocean): Morpho-molecular characterization, toxin profile and response to light intensity. |
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| Authors: | Sibat M; Ifremer, ODE/PHYTOX-METALG, Rue de l'île d'Yeu, F-44300 Nantes, France. Electronic address: manoella.sibat@ifremer.fr., Mai T; Ifremer, IRD, Univ Nouvelle-Calédonie, Univ La Réunion, CNRS, UMR 9220 ENTROPIE, BP 32078, 98800, Noumea, New Caledonia; Institut Louis Malardé (ILM), 98713 Papeete, Tahiti, French Polynesia. Electronic address: tmai@ilm.pf., Chomérat N; Ifremer, ODE/COAST/LERBO, Station Ifremer de Concarneau, Place de la Croix, Concarneau, F-29900, France. Electronic address: nicolas.chomerat@ifremer.fr., Bilien G; Ifremer, ODE/COAST/LERBO, Station Ifremer de Concarneau, Place de la Croix, Concarneau, F-29900, France. Electronic address: gwenael.bilien@ifremer.fr., Lhaute K; Ifremer, ODE/PHYTOX-METALG, Rue de l'île d'Yeu, F-44300 Nantes, France. Electronic address: korian.lhaute@ifremer.fr., Hess P; Ifremer, ODE/PHYTOX-METALG, Rue de l'île d'Yeu, F-44300 Nantes, France; Ifremer, PHYTOX, Laboratoire PHYSALG, F-44300 Nantes, France. Electronic address: philipp.hess@ifremer.fr., Séchet V; Ifremer, PHYTOX, Laboratoire PHYSALG, F-44300 Nantes, France. Electronic address: veronique.sechet@ifremer.fr., Jauffrais T; Ifremer, IRD, Univ Nouvelle-Calédonie, Univ La Réunion, CNRS, UMR 9220 ENTROPIE, BP 32078, 98800, Noumea, New Caledonia. Electronic address: thierry.jauffrais@ifremer.fr. |
| Source: | Harmful algae [Harmful Algae] 2025 May; Vol. 145, pp. 102859. Date of Electronic Publication: 2025 Apr 09. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101128968 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1470 (Electronic) Linking ISSN: 15689883 NLM ISO Abbreviation: Harmful Algae Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| MeSH Terms: | Dinoflagellida*/genetics , Dinoflagellida*/classification , Dinoflagellida*/radiation effects , Dinoflagellida*/isolation & purification , Dinoflagellida*/chemistry , Dinoflagellida*/physiology , Ciguatoxins*/metabolism , Ciguatoxins*/analysis , Ciguatoxins*/chemistry , Light*, New Caledonia ; Tandem Mass Spectrometry ; Phylogeny ; Humans ; Chromatography, Liquid ; Cell Line, Tumor |
| Abstract: | Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Gambierdiscus is known to produce neurotoxins associated with ciguatera poisoning (CP). In New Caledonia (NC), South West Pacific Ocean, there is currently a significant knowledge gap regarding CP and the microalgae linked to this foodborne illness. This study describes a new strain of Gambierdiscus polynesiensis, 19PV93, isolated from the west coast of NC. The strain was isolated and cultured for morpho-molecular characterization to determine its phylogenetic position. Toxic activity was assessed using a cell-based assay with neuroblastoma cells (CBA-N2a), and the toxin profile was characterized using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to evaluate potential risks to human health. Regarding the toxin profile, G. polynesiensis was characterized by the presence of gambierone, 44-methylgambierone (44-MeG), and an atypical ciguatoxin profile consisting solely of ciguatoxin-4A (CTX4A) and -4B (CTX4B). This finding confirms intraspecific variations in toxin profiles between strains from different geographic origins. In culture, G. polynesiensis demonstrated a preference for relatively low irradiances (50 to 100 µmol photons m⁻² s⁻¹) compared to the higher light intensities often encountered in their natural environment. The impact of light on toxin concentrations was found to be inversely related to light intensity, with higher quotas observed at lower light levels. Gambierdiscus employed non-photochemical quenching as a photoprotective strategy to safeguard PSII from excessive light, particularly during both short-term and long-term exposure. However, this dissipation strategy alone appears insufficient, as photoinhibition was consistently observed, and the electron transfer rate and yield along the electron transfer chain rapidly declined with increasing light intensity. (Copyright © 2025 The Author(s). Published by Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: Chlorophyll a fluorescence; Ciguatoxins; Gambierdiscus; Harmful algal bloom; Liquid chromatography-tandem mass spectrometry; PAM fluorometry; Phylogeny |
| Substance Nomenclature: | 11050-21-8 (Ciguatoxins) |
| Entry Date(s): | Date Created: 20250505 Date Completed: 20250506 Latest Revision: 20250505 |
| Update Code: | 20250506 |
| DOI: | 10.1016/j.hal.2025.102859 |
| PMID: | 40324860 |
| Database: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br />Gambierdiscus is known to produce neurotoxins associated with ciguatera poisoning (CP). In New Caledonia (NC), South West Pacific Ocean, there is currently a significant knowledge gap regarding CP and the microalgae linked to this foodborne illness. This study describes a new strain of Gambierdiscus polynesiensis, 19PV93, isolated from the west coast of NC. The strain was isolated and cultured for morpho-molecular characterization to determine its phylogenetic position. Toxic activity was assessed using a cell-based assay with neuroblastoma cells (CBA-N2a), and the toxin profile was characterized using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) to evaluate potential risks to human health. Regarding the toxin profile, G. polynesiensis was characterized by the presence of gambierone, 44-methylgambierone (44-MeG), and an atypical ciguatoxin profile consisting solely of ciguatoxin-4A (CTX4A) and -4B (CTX4B). This finding confirms intraspecific variations in toxin profiles between strains from different geographic origins. In culture, G. polynesiensis demonstrated a preference for relatively low irradiances (50 to 100 µmol photons m⁻² s⁻¹) compared to the higher light intensities often encountered in their natural environment. The impact of light on toxin concentrations was found to be inversely related to light intensity, with higher quotas observed at lower light levels. Gambierdiscus employed non-photochemical quenching as a photoprotective strategy to safeguard PSII from excessive light, particularly during both short-term and long-term exposure. However, this dissipation strategy alone appears insufficient, as photoinhibition was consistently observed, and the electron transfer rate and yield along the electron transfer chain rapidly declined with increasing light intensity.<br /> (Copyright © 2025 The Author(s). Published by Elsevier B.V. All rights reserved.) |
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| ISSN: | 1878-1470 |
| DOI: | 10.1016/j.hal.2025.102859 |