Inter-tissue glycan heterogeneity: site-specific glycoform analysis of mouse tissue N-glycoproteomes using MS1-based glycopeptide detection method assisted by lectin microarray.
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| Titel: | Inter-tissue glycan heterogeneity: site-specific glycoform analysis of mouse tissue N-glycoproteomes using MS1-based glycopeptide detection method assisted by lectin microarray. |
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| Autoren: | Nagai-Okatani C; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan. chiaki-okatani@aist.go.jp., Tomioka A; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan., Tominaga D; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan., Sakaue H; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan., Kuno A; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan., Kaji H; Molecular and Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8565, Japan. kaji.hiroyuki.j8@f.mail.nagoya-u.ac.jp.; Institute for Glyco-core Research (iGCORE), Nagoya University, Furo-Cho, Chikusa, Nagoya, Aichi, 464-8601, Japan. kaji.hiroyuki.j8@f.mail.nagoya-u.ac.jp. |
| Quelle: | Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2025 Feb; Vol. 417 (5), pp. 973-988. Date of Electronic Publication: 2024 Dec 16. |
| Publikationsart: | Journal Article |
| Sprache: | English |
| Info zur Zeitschrift: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Heidelberg : Springer-Verlag, 2002- |
| MeSH-Schlagworte: | Glycopeptides*/analysis , Glycopeptides*/chemistry , Polysaccharides*/analysis , Polysaccharides*/chemistry , Lectins*/chemistry , Glycoproteins*/chemistry , Glycoproteins*/analysis , Protein Array Analysis*/methods , Proteome*, Animals ; Mice ; Glycosylation ; Male ; Proteomics/methods ; Tandem Mass Spectrometry/methods |
| Abstract: | To understand the biological and pathological functions of protein glycosylation, the glycan heterogeneities for each glycosite in a single glycoprotein need to be elucidated depending on the type and status of cells. For this aim, a reliable strategy is needed to compare site-specific glycoforms of multiple glycoprotein samples in a comprehensive manner. To analyze this "inter-heterogeneity" of samples, we previously introduced an MS1-based glycopeptide detection method, "Glyco-RIDGE." Here, to elucidate inter-tissue glycan heterogeneities, this estimation method was applied to site-specific N-glycoforms of glycoproteins from six normal mouse tissues (liver, kidney, spleen, pancreas, stomach, and testis). The analyses of desialylated glycopeptides estimated 11,325 site-specific N-glycoforms with 239 glycan compositions at 1260 sites (1122 non-redundant core peptides) in 800 glycoproteins, revealing inter-tissue differences in macro-, micro-, and meta-glycan heterogeneities. To obtain detailed information on their glycan features and tissue distribution, the Glyco-RIDGE results were compared with laser microdissection-assisted lectin microarray (LMD-LMA)-based mouse tissue glycome mapping data deposited on LM-GlycomeAtlas, as well as MS-based mouse tissue glycome data deposited on GlycomeAtlas. This integrated approach supported the certainty of Glyco-RIDGE results and suggested that inter-tissue differences exist in glycan motifs, such as alpha-galactose and bisecting N-acetylglucosamine, in both whole tissue glycomes and specific glycoproteins, Anpep and Lamc1. In addition, the comparison with LMD-LMA-based tissue glycome mapping data suggested the possibility of estimating the tissue distribution of the assigned glycans and glycopeptides. Taken together, these findings demonstrate the utility of an integrated approach using MS assisted by LMA for large-scale analyses. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.) |
| Competing Interests: | Declarations. Ethics approval, source of biological material, and statement on animal welfare: C57BL/6 J mice were purchased from Japan SLC (Shizuoka, Japan). All animal experiments were performed in accordance with the relevant guidelines and regulations. The animal study protocol was approved by the Institutional Animal Care and Use Committee of AIST (no. 2022–082; date June approval, 22/06/2022). Conflict of interest: The authors declare no competing interests. |
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| Grant Information: | JP20ae0101021h0005 Japan Agency for Medical Research and Development; JP15K14426 Japan Society for the Promotion of Science |
| Contributed Indexing: | Keywords: Glycan heterogeneity; Glycoproteomics; Lectin microarray; Mass spectrometry; Site-specific glycoform; Tissue glycome mapping |
| Substance Nomenclature: | 0 (Glycopeptides) 0 (Polysaccharides) 0 (Lectins) 0 (Glycoproteins) 0 (Proteome) |
| Entry Date(s): | Date Created: 20241215 Date Completed: 20250427 Latest Revision: 20250427 |
| Update Code: | 20250428 |
| DOI: | 10.1007/s00216-024-05686-y |
| PMID: | 39676134 |
| Datenbank: | MEDLINE |
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