High-throughput peptide array analysis and computational techniques for serological profiling of flavivirus infections: Implications for diagnostics and vaccine development.
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| Název: | High-throughput peptide array analysis and computational techniques for serological profiling of flavivirus infections: Implications for diagnostics and vaccine development. |
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| Autoři: | Bombaci M; Istituto Nazionale Genetica Molecolare, Padiglione Romeo ed Enrica Invernizzi, Milano, Italy., Fassi EMA; Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Milano, Italy., Gobbini A; Istituto Nazionale Genetica Molecolare, Padiglione Romeo ed Enrica Invernizzi, Milano, Italy., Mileto D; Laboratory of Clinical Microbiology, Virology and Bioemergencies, ASST Fatebenefratelli Sacco - L. Sacco Hospital, Milano, Italy., Cassaniti I; Department of Clinical, Surgical, Diagnostics and Pediatric Sciences, University of Pavia, Pavia, Italy.; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Pesce E; Istituto Nazionale Genetica Molecolare, Padiglione Romeo ed Enrica Invernizzi, Milano, Italy.; Department of Clinical Sciences and Community Health, Department of Excellence 2023-2027, University of Milano, Milano, Italy., Casali E; Department of Chemistry, University of Pavia, Pavia, Italy., Mancon A; Laboratory of Clinical Microbiology, Virology and Bioemergencies, ASST Fatebenefratelli Sacco - L. Sacco Hospital, Milano, Italy., Sammartino J; Department of Clinical, Surgical, Diagnostics and Pediatric Sciences, University of Pavia, Pavia, Italy., Ferrari A; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Percivalle E; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Grande R; Laboratory of Clinical Microbiology, Virology and Bioemergencies, ASST Fatebenefratelli Sacco - L. Sacco Hospital, Milano, Italy., Marchisio E; DIA.PRO Diagnostic Bioprobes Srl, Sesto San Giovanni, Michigan, USA., Gismondo MR; Laboratory of Clinical Microbiology, Virology and Bioemergencies, ASST Fatebenefratelli Sacco - L. Sacco Hospital, Milano, Italy.; Department of Biomedical and Clinical Sciences, Università degli Studi di Milano, Milano, Italy., Abrignani S; Istituto Nazionale Genetica Molecolare, Padiglione Romeo ed Enrica Invernizzi, Milano, Italy.; Department of Clinical Sciences and Community Health, Department of Excellence 2023-2027, University of Milano, Milano, Italy., Baldanti F; Department of Clinical, Surgical, Diagnostics and Pediatric Sciences, University of Pavia, Pavia, Italy.; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Colombo G; Department of Chemistry, University of Pavia, Pavia, Italy., Grifantini R; Istituto Nazionale Genetica Molecolare, Padiglione Romeo ed Enrica Invernizzi, Milano, Italy. |
| Zdroj: | Journal of medical virology [J Med Virol] 2024 Sep; Vol. 96 (9), pp. e29923. |
| Způsob vydávání: | Journal Article |
| Jazyk: | English |
| Informace o časopise: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York Ny : Wiley-Liss Original Publication: New York, Liss. |
| Výrazy ze slovníku MeSH: | Flavivirus Infections*/diagnosis , Flavivirus Infections*/immunology , Flavivirus*/immunology , Protein Array Analysis*/methods, Humans ; Peptides/immunology ; Vaccine Development ; Computational Biology/methods ; Dengue/diagnosis ; Dengue/immunology ; Dengue/blood ; Dengue Virus/immunology ; Dengue Virus/genetics ; High-Throughput Screening Assays/methods ; Serologic Tests/methods ; Biomarkers/blood ; Viral Proteins/immunology ; Adult ; Antibodies, Viral/blood ; Middle Aged ; Male ; Female ; Zika Virus/immunology |
| Abstrakt: | Arthropod-borne viruses, such as dengue virus (DENV), pose significant global health threats, with DENV alone infecting around 400 million people annually and causing outbreaks beyond endemic regions. This study aimed to enhance serological diagnosis and discover new drugs by identifying immunogenic protein regions of DENV. Utilizing a comprehensive approach, the study focused on peptides capable of distinguishing DENV from other flavivirus infections through serological analyses. Over 200 patients with confirmed arbovirus infection were profiled using high-density pan flavivirus peptide arrays comprising 6253 peptides and the computational method matrix of local coupling energy (MLCE). Twenty-four peptides from nonstructural and structural viral proteins were identified as specifically recognized by individuals with DENV infection. Six peptides were confirmed to distinguish DENV from Zika virus (ZIKV), West Nile virus (WNV), Yellow Fever virus (YFV), Usutu virus (USUV), and Chikungunya virus (CHIKV) infections, as well as healthy controls. Moreover, the combination of two immunogenic peptides emerged as a potential serum biomarker for DENV infection. These peptides, mapping to highly accessible regions on protein structures, show promise for diagnostic and prophylactic strategies against flavivirus infections. The described methodology holds broader applicability in the serodiagnosis of infectious diseases. (© 2024 Wiley Periodicals LLC.) |
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| Grant Information: | Ministero della Salute; European Commission; Fondazione Romeo ed Enrica Invernizzi; Regione Lombardia |
| Contributed Indexing: | Keywords: dengue virus; epitope prediction; flavivirus; peptides; protein arrays; serodiagnosis |
| Substance Nomenclature: | 0 (Peptides) 0 (Biomarkers) 0 (Viral Proteins) 0 (Antibodies, Viral) |
| Entry Date(s): | Date Created: 20240918 Date Completed: 20240918 Latest Revision: 20240918 |
| Update Code: | 20250114 |
| DOI: | 10.1002/jmv.29923 |
| PMID: | 39291820 |
| Databáze: | MEDLINE |
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