Induction of resistance mechanisms in Rhodotorula toruloides for growth in sugarcane hydrolysate with high inhibitor content.
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| Title: | Induction of resistance mechanisms in Rhodotorula toruloides for growth in sugarcane hydrolysate with high inhibitor content. |
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| Authors: | Lopes HJS; Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas, SP, 13083-852, Brazil., Bonturi N; Institute of Technology, University of Tartu, Tartu, Estonia., Miranda EA; Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas, SP, 13083-852, Brazil. everson@unicamp.br. |
| Source: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2021 Dec; Vol. 105 (24), pp. 9261-9272. Date of Electronic Publication: 2021 Nov 11. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin ; New York : Springer International, c1984- |
| MeSH Terms: | Rhodotorula* , Saccharum*, Biomass ; Xylose |
| Abstract: | The oleaginous yeast Rhodotorula toruloides is a potential lipid producer for biodiesel production. However, this yeast shows growth inhibition due to harmful compounds when cultivated in hemicellulose hydrolysate. Here, we present a comparative analysis of colony selection and heterologous adaptive laboratory enhancement (ALE) strategies for obtaining robust strains. We implemented these ALE strategies for R. toruloides in a culture medium containing sugarcane hemicellulose hydrolysate. Our comparison study showed that the strain obtained with heterogeneous ALE strategy (Rth) reached a µ (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| Grant Information: | 152033/2015-7 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 2016/10636-8 Fundação de Amparo à Pesquisa do Estado de São Paulo |
| Contributed Indexing: | Keywords: Adaptive; Biodiesel; Hydrolysate; Inhibitors; Lipids; R. toruloides |
| Substance Nomenclature: | A1TA934AKO (Xylose) |
| SCR Organism: | Rhodotorula toruloides |
| Entry Date(s): | Date Created: 20211111 Date Completed: 20211208 Latest Revision: 20211214 |
| Update Code: | 20250114 |
| DOI: | 10.1007/s00253-021-11687-z |
| PMID: | 34761276 |
| Database: | MEDLINE |
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Nájsť tento článok vo Web of Science | Abstract: | The oleaginous yeast Rhodotorula toruloides is a potential lipid producer for biodiesel production. However, this yeast shows growth inhibition due to harmful compounds when cultivated in hemicellulose hydrolysate. Here, we present a comparative analysis of colony selection and heterologous adaptive laboratory enhancement (ALE) strategies for obtaining robust strains. We implemented these ALE strategies for R. toruloides in a culture medium containing sugarcane hemicellulose hydrolysate. Our comparison study showed that the strain obtained with heterogeneous ALE strategy (Rth) reached a µ <subscript>max</subscript> of 55% higher than the parental strain. It also exhibited higher biomass production (6.51 g/l) and lipid content (60%). ALE with colony selection strategy (Rtc) had a fitness gain in terms of shortening of the lag phase (9 h) when compared to Rth and parental strain (11.67, 12.33 h, respectively). When cultivated in Eucalyptus urograndis hemicellulose hydrolysate, the Rth strain achieved a high lipid content, 64%. Kinetics studies showed a strong effect of acetic acid as a repressor of xylose consumption during R. toruloides cultivation.Key points• Distinct adaptive laboratory strategies resulted in strains with different physiologies.• Heterologous adaptive laboratory enhancement provided the best results (fitness gain of 55% in µmax).• The Rth strain achieved a lipid content of 64.3% during cultivation in eucalyptus hemicellulose hydrolysate.<br /> (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| ISSN: | 1432-0614 |
| DOI: | 10.1007/s00253-021-11687-z |