Výsledky vyhledávání - "loop grafting"

Automated Engineering Protein Dynamics via Loop Grafting: Improving Renilla Luciferase Catalysis

Autoři: Planas-Iglesias, Joan Majerova, Marika Pluskal, Daniel a další

Zdroj: ACS Catal
ACS Catalysis

Témata: loop grafting, protein dynamics, bifunctionalprotein chimeras, Renilla luciferase, enzyme engineering, bioluminescent catalytic activity

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/40013243

Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development

Autoři: Pramila Rijal Leiyan Wei Guido C Paesen a další

Zdroj: eLife, Vol 14 (2025)

Témata: influenza virus, neuraminidase, protein expression, loop grafting, vaccine, Medicine, Science, Biology (General), QH301-705.5

Popis souboru: electronic resource

Relation: https://elifesciences.org/articles/105317; https://doaj.org/toc/2050-084X

Přístupová URL adresa: https://doaj.org/article/ce79935d8e784bcc99bf7da3c5b90fcc

Conversion of the OmpF Porin into a Device to Gather Amyloids on the E. coli Outer Membrane

Autoři: Sol Vendrell-Fernández Paloma Lozano-Picazo Paula Cuadros-Sánchez a další

Přispěvatelé: Sol Vendrell-Fernández Paloma Lozano-Picazo Paula Cuadros-Sánchez a další

Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
instname

Témata: Mammals, 0301 basic medicine, Amyloid, 0303 health sciences, Prions, Porins, OmpF, Amyloid adhesins, 3. Good health, Porin loop grafting, 03 medical and health sciences, Escherichia coli, Animals, Peptides, Homotypic amyloid recognition, Amyloid and prion bioremediation, Bacterial Outer Membrane Proteins

Přístupová URL adresa: https://digital.csic.es/bitstream/10261/256818/1/acssynbio_vendrell-fern%c3%a1ndez_2021.pdf
https://pubmed.ncbi.nlm.nih.gov/34852197
http://hdl.handle.net/10261/256818
https://pubs.acs.org/doi/10.1021/acssynbio.1c00347
https://pubmed.ncbi.nlm.nih.gov/34852197/
https://www.ncbi.nlm.nih.gov/pubmed/34852197

Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development.

Autoři: Rijal, Pramila Wei, Leiyan Paesen, Guido C a další

Témata: infectious disease, influenza, influenza virus, loop grafting, microbiology, molecular biophysics, neuraminidase, protein expression, structural biology, vaccine, viruses, Animals, Influenza Vaccines, Mice, Humans, Vaccine Development, Inbred BALB C, Antibodies, Viral, Monoclonal, Orthomyxoviridae Infections, Female, Viral Proteins, Protein Conformation

Popis souboru: text/xml; application/zip; application/pdf

Relation: 105317; https://www.repository.cam.ac.uk/handle/1810/389321

Dostupnost: https://www.repository.cam.ac.uk/handle/1810/389321

Insertions and deletions in protein evolution and engineering

Autoři: Savino, Simone Desmet, Tom Franceus, Jorick

Zdroj: BIOTECHNOLOGY ADVANCES ; ISSN: 0734-9750 ; ISSN: 1873-1899

Témata: Biology and Life Sciences, Technology and Engineering, Biotechnology, Bioengineering, Applied Microbiology and Biotechnology, Indel, Directed evolution, Enzyme engineering, Frameshift mutation, Polymerase slippage, Loop grafting, AMINO-ACID DELETION, SUBSTRATE SPECTRUM EXTENSION, SATURATION MUTAGENESIS, SCANNING MUTAGENESIS, MUTATION-RATE, INDEL RATES, LOOP, PHOSPHOTRIESTERASE, ENZYMES

Popis souboru: application/pdf

Relation: https://biblio.ugent.be/publication/01GSQSHVPHGZEHX6VP3VYQ5N86; http://doi.org/10.1016/j.biotechadv.2022.108010; https://biblio.ugent.be/publication/01GSQSHVPHGZEHX6VP3VYQ5N86/file/01GSQSJHGXV946F3S22GKK6H65

Dostupnost: https://biblio.ugent.be/publication/01GSQSHVPHGZEHX6VP3VYQ5N86
http://hdl.handle.net/1854/LU-01GSQSHVPHGZEHX6VP3VYQ5N86
https://doi.org/10.1016/j.biotechadv.2022.108010
https://biblio.ugent.be/publication/01GSQSHVPHGZEHX6VP3VYQ5N86/file/01GSQSJHGXV946F3S22GKK6H65

EVOLVING THE ENZYME THROUGH LOOP ENGINEERING

Autoři: Kim, Hak-Sung

Zdroj: Enzyme Engineering XXVII

Témata: Enzyme evolution, loop grafting, scaffold, diversity

Relation: https://dc.engconfintl.org/enzyme_xxvii/69; https://dc.engconfintl.org/context/enzyme_xxvii/article/1048/filename/0/type/additional/viewcontent/48.pdf

Dostupnost: https://dc.engconfintl.org/enzyme_xxvii/69
https://dc.engconfintl.org/context/enzyme_xxvii/article/1048/filename/0/type/additional/viewcontent/48.pdf

Antibody Modeling: Implications for Engineering and Design

Autoři: Morea V Lesk Tramontano a další

Zdroj: Methods. 20:267-279

Témata: SEQUENCE ALIGNMENT, Models, Molecular, 0301 basic medicine, 0303 health sciences, Binding Sites, MONOCLONAL-ANTIBODY, CANONICAL STRUCTURES, Protein Conformation, protein engineering, canonical structures, RESHAPING HUMAN-ANTIBODIES, PROTEIN STRUCTURES, Protein Engineering, antibody modeling, Antibodies, loop grafting, 3. Good health, BETA-HAIRPINS, 03 medical and health sciences, GLOBULAR-PROTEINS, HYPERVARIABLE REGION, CDRs, ANTIGEN INTERACTIONS, 3-DIMENSIONAL STRUCTURE

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/10694450
https://pennstate.pure.elsevier.com/en/publications/antibody-modeling-implications-for-engineering-and-design
https://www.sciencedirect.com/science/article/pii/S1046202399909210
http://www.ncbi.nlm.nih.gov/pubmed/10694450
http://www.sciencedirect.com/science/article/pii/S1046202399909210
https://pubmed.ncbi.nlm.nih.gov/10694450/
https://hdl.handle.net/20.500.14243/198502
https://doi.org/10.1006/meth.1999.0921

Engineering a nanopore with co-chaperonin function

Autoři: Ho, Ching-Wen Meervelt, Veerle Tsai, Keng-Chang a další

Zdroj: Ho, C-W, Meervelt, V, Tsai, K-C, De Temmerman, P-J, Mast, J & Maglia, G 2015, 'Engineering a nanopore with co-chaperonin function', Science Advances, vol. 1, no. 11, e1500905. https://doi.org/10.1126/sciadv.1500905

Témata: single-molecule, protein folding, nanomachine, loop grafting, GroEL, GroES

Popis souboru: application/pdf

Dostupnost: https://hdl.handle.net/11370/0efd1dc6-ccba-4157-b83d-508952df956f
https://research.rug.nl/en/publications/0efd1dc6-ccba-4157-b83d-508952df956f
https://doi.org/10.1126/sciadv.1500905
https://pure.rug.nl/ws/files/55434034/Engineering_a_nanopore_with_co_chaperonin_function.pdf

Computational design gains momentum in enzyme catalysis engineering

Autoři: Wijma, Hein J. Janssen, Dick B.

Zdroj: Febs Journal. 280(13):2948-2960

Témata: biocatalysis, SEQUENCE LIBRARY, substrate specificity, DIRECTED EVOLUTION, CLEAVAGE SPECIFICITY, enzymatic activity, HOMING ENDONUCLEASES, computer-aided enzyme design, insilico design, stability, RATIONAL PROTEIN DESIGN, loop grafting, TRANSITION-STATES, ranking, HUMAN BUTYRYLCHOLINESTERASE, LIGAND-BINDING, computer-aided protein design, DEAD-END ELIMINATION, SIDE-CHAIN, substrate scope

Přístupová URL adresa: https://research.rug.nl/en/publications/1d8d2104-514b-4c38-bcea-8a95923c1ac2
https://hdl.handle.net/11370/1d8d2104-514b-4c38-bcea-8a95923c1ac2

Development and differentiation of hyperstable consensus monobodies based on the type 3 fibronectin domain

Autoři: PETER GEOFFREY CHANDLER

Témata: Structural biology (incl. macromolecular modelling), Biochemistry and cell biology not elsewhere classified, Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies), Medical biochemistry - proteins and peptides (incl. medical proteomics), consensus design, stability–function trade-off, loop grafting, mini proteins, monobodies, adnectin, non-antibody scaffold, fibronectin, Biochemistry, Structural Biology, Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)

Relation: https://figshare.com/articles/thesis/Development_and_differentiation_of_hyperstable_consensus_monobodies_based_on_the_type_3_fibronectin_domain/15051897

Dostupnost: https://doi.org/10.26180/15051897.v1
https://figshare.com/articles/thesis/Development_and_differentiation_of_hyperstable_consensus_monobodies_based_on_the_type_3_fibronectin_domain/15051897

Studies on variable surface loop regions of the ene reductase NCR from Zymomonas mobilis ; Studien über variable Oberflächenloops der En-Reduktase NCR aus Zymomonas mobilis

Autoři: Reich, Sabrina Hauer, Bernhard (Prof. Dr.)

Přispěvatelé: Reich, Sabrina Hauer, Bernhard (Prof. Dr.)

Témata: Zymomonas mobilis, Old Yellow Enzyme, Reduktion von aktivierten Kohlenstoffdoppelbindungen, Loop grafting, Golden Gate Shuffling, bioinformatische Loopanalyse, Old Yellow Enzymes, reduction of activated carbon double bonds, bioinformatic loop analysis

Time: 570

Popis souboru: application/pdf

Relation: 406540012

Dostupnost: https://doi.org/10.18419/opus-1416
http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-92264
http://elib.uni-stuttgart.de/handle/11682/1433

Studies on variable surface loop regions of the ene reductase NCR from Zymomonas mobilis

Autoři: Reich, Sabrina Hauer, Bernhard

Přispěvatelé: Reich, Sabrina Hauer, Bernhard

Dostupnost: http://data.europeana.eu/aggregation/europeana/2048441/item_ZTVZY3BE2ENDY5RJ4NJ5M7YKUQKMYBUG

Effect of loop-grafting on the activity, stability and regioselectivity of Priestia megaterium levansucrase using two inulosucrases as loop donors.

Autoři: Ortiz-Soto ME Schmid K Weber C a další

Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2025 May; Vol. 306 (Pt 3), pp. 141584. Date of Electronic Publication: 2025 Feb 27.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Hexosyltransferases*/chemistry , Hexosyltransferases*/metabolism , Hexosyltransferases*/genetics, Substrate Specificity ; Enzyme Stability ; Catalytic Domain ; Oligosaccharides/chemistry ; Fructans ; Inulin ; Kinetics ; Bacterial Proteins

Conversion of the OmpF porin into a device to gather amyloids on the E. coli outer membrane

Autoři: Ministerio de Ciencia e Innovación (España) European Commission Vendrell-Fernández, Sol [0000-0003-0153-8454] a další

Témata: OmpF, Porin loop grafting, Amyloid adhesins, Homotypic amyloid recognition, Amyloid and prion bioremediation, Escherichia coli, artículo

URL: http://hdl.handle.net/10261/256818
https://doi.org/10.1021/acssynbio.1c00347
Publisher's version
https://doi.org/10.1021/acssynbio.1c00347
Sí
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020MICIU/ICTI2017‐2020/RTI2018-094549-B-I00

Mutational and biophysical robustness in a prestabilized monobody.

Autoři: Chandler PG Tan LL Porebski BT a další

Zdroj: The Journal of biological chemistry [J Biol Chem] 2021 Jan-Jun; Vol. 296, pp. 100447. Date of Electronic Publication: 2021 Feb 20.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE

Výrazy ze slovníku MeSH: Antibodies/*metabolism , Fibronectin Type III Domain/*genetics, Antibodies/immunology ; Fibronectin Type III Domain/immunology ; Fibronectins/genetics ; Fibronectins/immunology ; Fibronectins/metabolism ; Genetic Engineering/methods ; Humans ; Matrix Attachment Regions ; Mutation ; Peptide Fragments/genetics ; Peptide Fragments/immunology ; Peptide Fragments/metabolism ; Protein Binding/genetics ; Protein Binding/immunology ; Vascular Endothelial Growth Factor Receptor-2/immunology ; Vascular Endothelial Growth Factor Receptor-2/metabolism

Enhancing subtilisin thermostability through a modified normalized B-factor analysis and loop-grafting strategy.

Autoři: Tang H Shi K Shi C a další

Zdroj: The Journal of biological chemistry [J Biol Chem] 2019 Nov 29; Vol. 294 (48), pp. 18398-18407. Date of Electronic Publication: 2019 Oct 15.

Způsob vydávání: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Informace o časopise: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE

Výrazy ze slovníku MeSH: Mutation* , Temperature*, Adenosine Monophosphate/*analogs & derivatives , Bacterial Proteins/*genetics , Enzyme Stability/*genetics , Subtilisin/*genetics, Adenosine Monophosphate/chemistry ; Adenosine Monophosphate/metabolism ; Algorithms ; Bacillus subtilis/enzymology ; Bacillus subtilis/genetics ; Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Circular Dichroism ; Crystallography, X-Ray ; Hydrogen Bonding ; Kinetics ; Molecular Dynamics Simulation ; Protein Conformation ; Subtilisin/chemistry ; Subtilisin/metabolism

Simultaneously improving the activity and thermostability of a new proline 4-hydroxylase by loop grafting and site-directed mutagenesis.

Autoři: Liu C Zhao J Liu J a další

Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2019 Jan; Vol. 103 (1), pp. 265-277. Date of Electronic Publication: 2018 Oct 12.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Mutagenesis, Site-Directed/*methods , Prolyl Hydroxylases/*chemistry , Prolyl Hydroxylases/*metabolism , Protein Engineering/*methods, Batch Cell Culture Techniques ; Enzyme Stability ; Escherichia coli/genetics ; Escherichia coli/growth & development ; Hydroxyproline/metabolism ; Kinetics ; Molecular Dynamics Simulation ; Prolyl Hydroxylases/genetics

A workflow for in silico design of hIL-10 and ebvIL-10 inhibitors using well-known miniprotein scaffolds.

Autoři: Dueñas S Aguila SA Pimienta G

Zdroj: Journal of molecular modeling [J Mol Model] 2017 Apr; Vol. 23 (4), pp. 118. Date of Electronic Publication: 2017 Mar 14.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Springer Country of Publication: Germany NLM ID: 9806569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0948-5023 (Electronic) Linking ISSN: 09485023 NLM ISO Abbreviation: J Mol Model Subsets: MEDLINE

Výrazy ze slovníku MeSH: Drug Design* , Molecular Docking Simulation*, Herpesvirus 4, Human/*metabolism , Interleukin-10/*antagonists & inhibitors, Computational Biology ; Humans ; Viral Proteins/antagonists & inhibitors ; Workflow

Circumventing the stability-function trade-off in an engineered FN3 domain.

Autoři: Porebski BT Conroy PJ Drinkwater N a další

Zdroj: Protein engineering, design & selection : PEDS [Protein Eng Des Sel] 2016 Nov 01; Vol. 29 (11), pp. 541-550.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Oxford University Press Country of Publication: England NLM ID: 101186484 Publication Model: Print Cited Medium: Internet ISSN: 1741-0134 (Electronic) Linking ISSN: 17410126 NLM ISO Abbreviation: Protein Eng Des Sel Subsets: PubMed not MEDLINE; MEDLINE

Loop-Grafted Old Yellow Enzymes in the Bienzymatic Cascade Reduction of Allylic Alcohols.

Autoři: Reich S Nestl BM Hauer B

Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2016 Apr 01; Vol. 17 (7), pp. 561-5. Date of Electronic Publication: 2016 Feb 23.

Způsob vydávání: Journal Article; Research Support, Non-U.S. Gov't

Informace o časopise: Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 100937360 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-7633 (Electronic) Linking ISSN: 14394227 NLM ISO Abbreviation: Chembiochem Subsets: MEDLINE

Výrazy ze slovníku MeSH: Models, Molecular*, NADPH Dehydrogenase/*metabolism , Propanols/*chemistry , Propanols/*metabolism, Alcohol Dehydrogenase/metabolism ; Amino Acid Sequence ; Molecular Structure ; NADPH Dehydrogenase/chemistry ; Oxidation-Reduction ; Sequence Alignment