Výsledky vyhľadávania - "fluorescence polarization"

A First Case of Fluorescence Polarization Biosensor-Based Assay for Rapid Monitoring of Protein API Content in Tablet Dosage Forms: Detection of Lysozyme in Tablets.

Autori: Filimonova SM Balyklova KS Zherdev DO a ďalší

Zdroj: Biosensors [Biosensors (Basel)] 2025 Nov 01; Vol. 15 (11). Date of Electronic Publication: 2025 Nov 01.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE

Výrazy zo slovníka MeSH: Muramidase*/analysis , Tablets*/chemistry , Biosensing Techniques*/methods , Fluorescence Polarization*/methods

CRISPR-Cas12a and DNA Tetrahedron Assemblies Amplified Fluorescence Anisotropy for the Sensitive Detection of Hepatitis B Virus DNA.

Autori: Qin Y Xie J Zhen S

Zdroj: Biosensors [Biosensors (Basel)] 2025 Oct 17; Vol. 15 (10). Date of Electronic Publication: 2025 Oct 17.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE

Výrazy zo slovníka MeSH: Hepatitis B virus*/genetics , DNA, Viral*/analysis , Fluorescence Polarization*/methods , CRISPR-Cas Systems* , Biosensing Techniques*, Humans ; Limit of Detection ; Hepatitis B

A label-free fluorescence polarization technology for the detection of food additives.

Autori: Fan S Wang J Wen X a ďalší

Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2026 Jan 05; Vol. 344 (Pt 1), pp. 126634. Date of Electronic Publication: 2025 Jul 01.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: England NLM ID: 9602533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3557 (Electronic) Linking ISSN: 13861425 NLM ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization*/methods , Food Additives*/analysis, Serum Albumin, Bovine/chemistry ; Manganese Compounds/chemistry ; Oxides/chemistry ; Choline/analysis ; Animals ; Glucose/analysis ; Cattle ; Hydrogen Peroxide/chemistry ; Nanoparticles/chemistry ; Nanoparticles/ultrastructure ; Limit of Detection ; Lactic Acid/analysis

A versatile fluorescence polarization-based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim).

Autori: Zhang J Allen J Ward SJ a ďalší

Zdroj: The Journal of biological chemistry [J Biol Chem] 2025 Jul; Vol. 301 (7), pp. 110342. Date of Electronic Publication: 2025 Jun 06.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization*/methods , Peptides*/chemistry , Peptides*/metabolism , Ubiquitin-Specific Proteases*/metabolism , Ubiquitin-Specific Proteases*/antagonists & inhibitors , Ubiquitin-Specific Proteases*/chemistry, Humans ; Ubiquitin/metabolism ; Ubiquitin/chemistry ; Substrate Specificity ; Ubiquitination ; Ubiquitin Thiolesterase/metabolism ; Ubiquitin Thiolesterase/antagonists & inhibitors

Design of a Fluorescence Polarization Probe for Enterovirus 2C Proteins.

Autori: Li K Demssie HA Wang J

Zdroj: Journal of medicinal chemistry [J Med Chem] 2025 Jul 10; Vol. 68 (13), pp. 14041-14053. Date of Electronic Publication: 2025 Jun 21.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: American Chemical Society Country of Publication: United States NLM ID: 9716531 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4804 (Electronic) Linking ISSN: 00222623 NLM ISO Abbreviation: J Med Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization* , Antiviral Agents*/pharmacology , Antiviral Agents*/chemistry , Antiviral Agents*/chemical synthesis , Drug Design* , Viral Nonstructural Proteins*/antagonists & inhibitors , Viral Nonstructural Proteins*/metabolism , Viral Nonstructural Proteins*/chemistry , Fluorescent Dyes*/chemistry , Fluorescent Dyes*/chemical synthesis , Fluorescent Dyes*/pharmacology , Enterovirus*/drug effects , Enterovirus*/metabolism, Humans ; Structure-Activity Relationship ; Carrier Proteins

Design, synthesis, and evaluation of a novel fluorescent probe for competitive fluorescence polarization assay to screen galectin-8 inhibitors.

Autori: Purić E Marinović M Kojek Z a ďalší

Zdroj: Acta pharmaceutica (Zagreb, Croatia) [Acta Pharm] 2025 Jul 03; Vol. 75 (2), pp. 273-282. Date of Electronic Publication: 2025 Jul 03 (Print Publication: 2025).

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Sciendo Country of Publication: Poland NLM ID: 9303678 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1846-9558 (Electronic) Linking ISSN: 13300075 NLM ISO Abbreviation: Acta Pharm Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescent Dyes*/chemical synthesis , Galectins*/antagonists & inhibitors , Fluorescence Polarization*, Drug Evaluation, Preclinical ; Molecular Structure

A novel CRISPR-Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing.

Autori: Qin Y Xie JL Mao K a ďalší

Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2025 May 15; Vol. 61 (41), pp. 7458-7461. Date of Electronic Publication: 2025 May 15.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 9610838 Publication Model: Electronic Cited Medium: Internet ISSN: 1364-548X (Electronic) Linking ISSN: 13597345 NLM ISO Abbreviation: Chem Commun (Camb) Subsets: MEDLINE

Výrazy zo slovníka MeSH: Biosensing Techniques*/methods , Fluorescence Polarization*/methods , CRISPR-Cas Systems* , DNA, Viral*/analysis , Hepatitis B virus*/genetics, DNA/chemistry ; Bacterial Proteins ; Endodeoxyribonucleases ; CRISPR-Associated Proteins

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules.

Autori: Kolimi N Ghimire S Duffy F a ďalší

Zdroj: Journal of visualized experiments : JoVE [J Vis Exp] 2025 Apr 25 (218). Date of Electronic Publication: 2025 Apr 25.

Spôsob vydávania: Journal Article; Video-Audio Media

Informácie o časopise: Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization*/methods , Forkhead Transcription Factors*/chemistry , Forkhead Transcription Factors*/metabolism , DNA*/chemistry , DNA*/metabolism , Repressor Proteins*/chemistry , Repressor Proteins*/metabolism , Single Molecule Imaging*/methods, Microscopy, Confocal/methods

Direct Fluorescence Anisotropy Detection of miRNA Based on Duplex-Specific Nuclease Signal Amplification.

Autori: Wu H Ling X Huang S a ďalší

Zdroj: Analytical chemistry [Anal Chem] 2025 Apr 22; Vol. 97 (15), pp. 8574-8580. Date of Electronic Publication: 2025 Apr 09.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: MicroRNAs*/analysis , MicroRNAs*/genetics , Fluorescence Polarization*/methods, Humans ; Fluorescent Dyes/chemistry ; Rhodamines/chemistry ; Limit of Detection ; DNA, Single-Stranded/chemistry

Fine-tuning probes for fluorescence polarization binding assays of bivalent ligands against polo-like kinase 1 using full-length protein.

Autori: Tsuji K Tamamura H Burke TR

Zdroj: Bioorganic & medicinal chemistry [Bioorg Med Chem] 2025 Mar 01; Vol. 119, pp. 118055. Date of Electronic Publication: 2024 Dec 28.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Science Country of Publication: England NLM ID: 9413298 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-3391 (Electronic) Linking ISSN: 09680896 NLM ISO Abbreviation: Bioorg Med Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Polo-Like Kinase 1* , Protein Serine-Threonine Kinases*/metabolism , Protein Serine-Threonine Kinases*/antagonists & inhibitors , Protein Serine-Threonine Kinases*/chemistry , Proto-Oncogene Proteins*/antagonists & inhibitors , Proto-Oncogene Proteins*/metabolism , Proto-Oncogene Proteins*/chemistry , Cell Cycle Proteins*/metabolism , Cell Cycle Proteins*/antagonists & inhibitors , Cell Cycle Proteins*/chemistry , Fluorescence Polarization* , Protein Kinase Inhibitors*/pharmacology , Protein Kinase Inhibitors*/chemistry , Protein Kinase Inhibitors*/chemical synthesis, Humans ; Ligands ; Protein Binding ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/chemical synthesis ; Fluorescent Dyes/pharmacology ; Structure-Activity Relationship ; Molecular Structure ; Binding Sites

Fluorescence polarization binding assays for the E3 ligase FEM1C.

Autori: Seipp EK Huang R

Zdroj: Methods in enzymology [Methods Enzymol] 2025; Vol. 719, pp. 347-362. Date of Electronic Publication: 2025 Jul 11.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization*/methods , Ubiquitin-Protein Ligases*/metabolism , Carrier Proteins*/metabolism , Carrier Proteins*/chemistry , Carrier Proteins*/genetics , Cell Cycle Proteins*/metabolism , Cell Cycle Proteins*/genetics, Humans ; Protein Binding ; Recombinant Proteins/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/chemistry ; Fluorescent Dyes/chemistry ; Proteolysis

Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay.

Autori: Kuś K Vasiljeva L

Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2863, pp. 71-80.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization*/methods , Exoribonucleases*/metabolism, Humans ; RNA/metabolism ; Enzyme Assays/methods

Fluorescence Polarization Method to Assess the FOXO Phosphopeptide Interaction with 14-3-3.

Autori: Kumar V Lakshman PKC Kamariah N

Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2871, pp. 99-106.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Výrazy zo slovníka MeSH: 14-3-3 Proteins*/metabolism , 14-3-3 Proteins*/chemistry , Phosphopeptides*/metabolism , Phosphopeptides*/chemistry , Fluorescence Polarization*/methods , Protein Binding*, Humans ; Forkhead Box Protein O3/metabolism

Fluorescence polarization assays to study carbohydrate-protein interactions.

Autori: de Paz JL Nieto PM

Zdroj: Organic & biomolecular chemistry [Org Biomol Chem] 2025 Feb 26; Vol. 23 (9), pp. 2041-2058. Date of Electronic Publication: 2025 Feb 26.

Spôsob vydávania: Journal Article; Review

Informácie o časopise: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101154995 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-0539 (Electronic) Linking ISSN: 14770520 NLM ISO Abbreviation: Org Biomol Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Proteins*/chemistry , Proteins*/metabolism , Fluorescence Polarization*/methods , Carbohydrates*/chemistry, Fluorescent Dyes/chemistry ; Fluorescent Dyes/chemical synthesis ; Protein Binding ; Humans

Fluorescence Anisotropy for Monitoring cis- and trans-Membrane Interactions of Synaptotagmin-1.

Autori: Park Y; Neurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar. ypark@hbku.edu.qa.; College of Health & Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation, Doha, Qatar. ypark@hbku.edu.qa.

Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2887, pp. 175-182.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization*/methods , Synaptotagmin I*/metabolism , Synaptotagmin I*/chemistry , Cell Membrane*/metabolism, Liposomes/metabolism ; Liposomes/chemistry ; Calcium/metabolism ; Protein Binding ; Humans ; Animals

Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White.

Autori: Mukhametova LI Zherdev DO Eremin SA a ďalší

Zdroj: Biomolecules [Biomolecules] 2024 Dec 12; Vol. 14 (12). Date of Electronic Publication: 2024 Dec 12.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE

Výrazy zo slovníka MeSH: Muramidase*/chemistry , Muramidase*/metabolism , Oligosaccharides*/chemistry , Oligosaccharides*/metabolism , Oligosaccharides*/analysis , Chickens* , Egg White*/chemistry , Fluorescence Polarization*/methods, Animals ; Chitosan/chemistry ; Trisaccharides/metabolism ; Trisaccharides/chemistry

A cell-based scrambling assay reveals the phospholipid headgroup preference of TMEM16F on the plasma membrane.

Autori: Teo CF Tuomivaara ST van Hilten N a ďalší

Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2025 Nov 04; Vol. 122 (44), pp. e2516822122. Date of Electronic Publication: 2025 Oct 30.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE

Výrazy zo slovníka MeSH: Phospholipid Transfer Proteins*/metabolism , Phospholipid Transfer Proteins*/genetics , Cell Membrane*/metabolism , Anoctamins*/metabolism , Anoctamins*/genetics , Phospholipids*/metabolism , Phospholipids*/chemistry, Phosphatidylserines/metabolism ; Phosphatidylethanolamines/metabolism ; Humans ; Phosphatidylcholines/metabolism ; Animals ; Fluorescence Polarization

Click chemistry-based fluorescence polarization sensor for sensitive detection of ampicillin.

Autori: Zhang C Tian T Yin N a ďalší

Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Dec 15; Vol. 323, pp. 124872. Date of Electronic Publication: 2024 Jul 23.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: England NLM ID: 9602533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3557 (Electronic) Linking ISSN: 13861425 NLM ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Subsets: MEDLINE

Výrazy zo slovníka MeSH: Click Chemistry* , Ampicillin*/analysis , Graphite*/chemistry , Limit of Detection* , Aptamers, Nucleotide*/chemistry , Fluorescence Polarization*/methods, Animals ; Milk/chemistry ; Biosensing Techniques/methods ; Fluorescent Dyes/chemistry

Production of Natural Penicillin-Binding Protein 2 and Development of a Signal-Amplified Fluorescence Polarization Assay for the Determination of 28 Beta-Lactam Antibiotics in Milk.

Autori: Hu JJ Wang JP

Zdroj: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Dec 11; Vol. 72 (49), pp. 27528-27537. Date of Electronic Publication: 2024 Nov 25.

Spôsob vydávania: Journal Article; Evaluation Study

Informácie o časopise: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Milk*/chemistry , Anti-Bacterial Agents*/analysis , Anti-Bacterial Agents*/chemistry , beta-Lactams*/analysis , beta-Lactams*/chemistry , beta-Lactams*/metabolism , Penicillin-Binding Proteins*/metabolism , Penicillin-Binding Proteins*/chemistry , Food Contamination*/analysis , Fluorescence Polarization*/methods, Animals ; Molecular Docking Simulation ; Cattle ; Drug Residues/analysis ; Drug Residues/metabolism ; Drug Residues/chemistry ; Escherichia coli/metabolism ; Escherichia coli Proteins/metabolism ; Escherichia coli Proteins/chemistry ; Tandem Mass Spectrometry/methods ; beta Lactam Antibiotics

Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.

Autori: Dean WF Mattheyses AL

Zdroj: Journal of cell science [J Cell Sci] 2024 Oct 15; Vol. 137 (20). Date of Electronic Publication: 2024 Oct 14.

Spôsob vydávania: Journal Article; Review

Informácie o časopise: Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE

Výrazy zo slovníka MeSH: Fluorescence Polarization*/methods , Microscopy, Fluorescence*/methods, Humans ; Animals ; Cytoskeleton/metabolism