Search Results - "bioluminescence"

BRET-based G Protein Biosensors for Measuring G Protein-Coupled Receptor Activity in Live Cells.

Authors: Cœugnet A Schihada H Rascol E et al.

Source: Journal of visualized experiments : JoVE [J Vis Exp] 2025 Nov 07 (225). Date of Electronic Publication: 2025 Nov 07.

Publication Type: Journal Article; Video-Audio Media

Journal Info: Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE

MeSH Terms: Biosensing Techniques*/methods , Receptors, G-Protein-Coupled*/metabolism , Receptors, G-Protein-Coupled*/analysis , Bioluminescence Resonance Energy Transfer Techniques*/methods , Receptors, Adrenergic, beta-2*/metabolism, Humans ; HEK293 Cells

A Highly Sensitive BRET-Based Reporter for Live-Cell Detection of HIV-1 Protease Activity and Inhibitor Screening.

Authors: Centazzo M Djossou AV Pavan S et al.

Source: Viruses [Viruses] 2025 Oct 19; Vol. 17 (10). Date of Electronic Publication: 2025 Oct 19.

Publication Type: Journal Article

Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

MeSH Terms: HIV Protease*/metabolism , HIV Protease*/analysis , HIV Protease*/genetics , HIV-1*/enzymology , HIV-1*/drug effects , HIV Protease Inhibitors*/pharmacology , Genes, Reporter* , Bioluminescence Resonance Energy Transfer Techniques*/methods, Humans ; Drug Evaluation, Preclinical/methods ; Luminescent Proteins/genetics ; Cell Survival ; Luciferases, Renilla/genetics ; HEK293 Cells

Genetically encoded fluorogenic RNA-based bioluminescence resonance energy transfer (BRET) sensors for cellular imaging and target detection.

Authors: Mi L You M

Source: SLAS discovery : advancing life sciences R & D [SLAS Discov] 2025 Sep; Vol. 35, pp. 100243. Date of Electronic Publication: 2025 Jun 16.

Publication Type: Journal Article

Journal Info: Publisher: SAGE Publications Country of Publication: United States NLM ID: 101697563 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2472-5560 (Electronic) Linking ISSN: 24725552 NLM ISO Abbreviation: SLAS Discov Subsets: MEDLINE

MeSH Terms: RNA*/genetics , RNA*/chemistry , Biosensing Techniques*/methods , Fluorescent Dyes*/chemistry , Bioluminescence Resonance Energy Transfer Techniques*/methods , Molecular Imaging*/methods , Fluorescence Resonance Energy Transfer*/methods, Humans ; Single-Cell Analysis/methods ; Luminescent Measurements/methods ; Animals

Real-time monitoring with iTLR4 assay identifies ligand-dependent TLR4-TLR4 conformational dynamics.

Authors: Mustafa S Evans SG Hutchinson MR

Source: Frontiers in immunology [Front Immunol] 2025 Sep 01; Vol. 16, pp. 1580070. Date of Electronic Publication: 2025 Sep 01 (Print Publication: 2025).

Publication Type: Journal Article

Journal Info: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE

MeSH Terms: Toll-Like Receptor 4*/metabolism , Toll-Like Receptor 4*/chemistry , Bioluminescence Resonance Energy Transfer Techniques*/methods, Humans ; Lipopolysaccharides/pharmacology ; Lipopolysaccharides/immunology ; Ligands ; Signal Transduction ; HEK293 Cells ; Protein Conformation ; Immunity, Innate

Development of a HiBiT Peptide-Based NanoBRET Ligand Binding Assay for Galanin Receptor 1 in Live Cells.

Authors: Zhu H Daly HC Yang TG et al.

Source: ACS chemical biology [ACS Chem Biol] 2025 Jul 18; Vol. 20 (7), pp. 1594-1608. Date of Electronic Publication: 2025 Jul 04.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101282906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8937 (Electronic) Linking ISSN: 15548929 NLM ISO Abbreviation: ACS Chem Biol Subsets: MEDLINE

MeSH Terms: Galanin*/metabolism , Galanin*/chemistry , Bioluminescence Resonance Energy Transfer Techniques*/methods , Peptides*/chemistry , Peptides*/metabolism, Humans ; Ligands ; Protein Binding ; HEK293 Cells ; Boron Compounds/chemistry ; Animals ; Fluorescent Dyes/chemistry

Dual compartment utility of BRET-based biosensors for PPP2R5A/B56α, a cancer-associated B regulatory subunit of PP2A.

Authors: Yamauchi H Oishi A Ajiro M et al.

Source: BioTechniques [Biotechniques] 2025 Apr; Vol. 77 (4), pp. 153-163. Date of Electronic Publication: 2025 Jun 27.

Publication Type: Journal Article

Journal Info: Publisher: Taylor & Francis Country of Publication: England NLM ID: 8306785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1940-9818 (Electronic) Linking ISSN: 07366205 NLM ISO Abbreviation: Biotechniques Subsets: MEDLINE

MeSH Terms: Protein Phosphatase 2*/metabolism , Protein Phosphatase 2*/analysis , Protein Phosphatase 2*/genetics , Biosensing Techniques*/methods , Bioluminescence Resonance Energy Transfer Techniques*/methods , Neoplasms*/metabolism, Humans ; Proto-Oncogene Proteins c-myc/metabolism ; HEK293 Cells

Protein-Protein Interactions of Odorant and G Protein-Coupled Receptors: A BRET Analysis Approach.

Authors: Choi J Koo J

Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2915, pp. 161-168.

Publication Type: Journal Article

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH Terms: Receptors, Odorant*/metabolism , Receptors, Odorant*/chemistry , Receptors, Odorant*/genetics , Receptors, G-Protein-Coupled*/metabolism , Receptors, G-Protein-Coupled*/chemistry , Bioluminescence Resonance Energy Transfer Techniques*/methods , Protein Interaction Mapping*/methods, Humans ; Protein Binding ; Signal Transduction ; HEK293 Cells ; Animals ; Protein Multimerization

Quantitative insights into protein turnover and ubiquitination with HiBiT and NanoBRET.

Authors: Piechota M Pokrzywa W

Source: Methods in enzymology [Methods Enzymol] 2025; Vol. 719, pp. 95-120. Date of Electronic Publication: 2025 Jul 11.

Publication Type: Journal Article

Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE

MeSH Terms: Ubiquitination* , Von Hippel-Lindau Tumor Suppressor Protein*/metabolism , Bioluminescence Resonance Energy Transfer Techniques*/methods , F-Box Proteins*/metabolism, Humans ; Proteolysis ; Protein Stability ; HEK293 Cells ; Ubiquitin-Protein Ligases/metabolism

Bioluminescence Resonance Energy Transfer (BRET) Assay to Measure Gq Recruitment to the Ghrelin Receptor.

Authors: Jamaluddin A; Metabolism and Systems Science, University of Birmingham, Birmingham, UK. a.jamaluddin@bham.ac.uk.; Centre of Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, Birmingham, UK. a.jamaluddin@bham.ac.uk.

Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2861, pp. 57-69.

Publication Type: Journal Article

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH Terms: Bioluminescence Resonance Energy Transfer Techniques*/methods , GTP-Binding Protein alpha Subunits, Gq-G11*/metabolism , Receptors, Ghrelin*/metabolism , Receptors, Ghrelin*/genetics, Humans ; HEK293 Cells ; Protein Binding ; Signal Transduction ; Transfection

Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer.

Authors: Duval CJ Steffen CL Pavic K et al.

Source: STAR protocols [STAR Protoc] 2024 Dec 20; Vol. 5 (4), pp. 103348. Date of Electronic Publication: 2024 Sep 28.

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE

MeSH Terms: Bioluminescence Resonance Energy Transfer Techniques*/methods, Humans ; HEK293 Cells ; Fluorescence Resonance Energy Transfer/methods ; Protein Interaction Mapping/methods ; Luminescent Measurements/methods

SHEDDING LIGHT ON Luminescence.

Authors: FINK, MAGGIE M. Rizk, Shahir S.

Source: Discover. Sep/Oct2025, Vol. 46 Issue 5, p48-53. 6p. 15 Color Photographs, 1 Black and White Photograph.

Subject Terms: *BIOLUMINESCENCE, *LUCIFERASES, *APPLIED sciences, *BIOLOGICAL evolution, *PREDATION, *SCIENTIFIC method, *BIOLUMINESCENCE assay, *SPECIES

Conformation- and activation-based BRET sensors differentially report on GPCR-G protein coupling.

Authors: Wright SC Avet C Gaitonde SA et al.

Source: Science signaling [Sci Signal] 2024 Jun 18; Vol. 17 (841), pp. eadi4747. Date of Electronic Publication: 2024 Jun 18.

Publication Type: Journal Article

Journal Info: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101465400 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1937-9145 (Electronic) Linking ISSN: 19450877 NLM ISO Abbreviation: Sci Signal Subsets: MEDLINE

MeSH Terms: Receptors, G-Protein-Coupled*/metabolism , Receptors, G-Protein-Coupled*/chemistry , Bioluminescence Resonance Energy Transfer Techniques*/methods , Biosensing Techniques*/methods, Humans ; HEK293 Cells ; Protein Conformation ; Signal Transduction ; GTP-Binding Proteins/metabolism ; GTP-Binding Proteins/chemistry ; GTP-Binding Proteins/genetics

Studying RAS Interactions in Live Cells with BRET.

Authors: Columbus J Turbyville T

Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2797, pp. 253-260.

Publication Type: Journal Article

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH Terms: Bioluminescence Resonance Energy Transfer Techniques*/methods , High-Throughput Screening Assays*, Energy Transfer ; Luminescent Measurements/methods

A novel BRET-based assay to investigate binding and residence time of unmodified ligands to the human lysosomal ion channel TRPML1 in intact cells.

Authors: Cunha MR Catta-Preta CMC Takarada JE et al.

Source: The Journal of biological chemistry [J Biol Chem] 2023 Jun; Vol. 299 (6), pp. 104807. Date of Electronic Publication: 2023 May 11.

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal Info: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE

MeSH Terms: Bioluminescence Resonance Energy Transfer Techniques*/methods , Transient Receptor Potential Channels*/metabolism, Humans ; Ligands ; Lysosomes/metabolism

Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology.

Authors: Pearce A Redfern-Nichols T Wills E et al.

Source: Journal of cell science [J Cell Sci] 2025 Jan 01; Vol. 138 (1). Date of Electronic Publication: 2025 Jan 15.

Publication Type: Journal Article; Review

Journal Info: Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE

MeSH Terms: Receptors, G-Protein-Coupled*/metabolism , Receptors, G-Protein-Coupled*/genetics , Signal Transduction*, Humans ; Animals ; beta-Arrestins/metabolism ; Cyclic AMP/metabolism ; Bioluminescence Resonance Energy Transfer Techniques

Highly Sensitive and Selective Detection of Inorganic Phosphates in the Water Environment by Biosensors Based on Bioluminescence Resonance Energy Transfer.

Authors: Yu J Zhang Y Zhao Y et al.

Source: Analytical chemistry [Anal Chem] 2023 Mar 21; Vol. 95 (11), pp. 4904-4913. Date of Electronic Publication: 2023 Mar 09.

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

MeSH Terms: Bioluminescence Resonance Energy Transfer Techniques*/methods , Biosensing Techniques*/methods, Phosphates ; Energy Transfer ; Luminescent Measurements/methods

Measuring Protein-Protein Interactions in Cells using Nanoluciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Assay.

Authors: Szewczyk MM Owens DDG Barsyte-Lovejoy D

Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2706, pp. 137-148.

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH Terms: Luminescent Measurements*/methods , Bioluminescence Resonance Energy Transfer Techniques*/methods, Reproducibility of Results ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Energy Transfer ; Biophysical Phenomena

Engineering autonomously luminescent plants using the fungal bioluminescence pathway.

Authors: Yu, Xiaolei Wang, Tiange Kong, Ci et al.

Source: New Phytologist. Dec2025, Vol. 248 Issue 5, p2251-2261. 11p.

Subject Terms: *BIOLUMINESCENCE, *BIOTECHNOLOGY, *AGRICULTURAL innovations, *BIOCHEMICAL engineering

A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants.

Authors: Ueno, Ryohei Ito, Shogo Oyama, Tokitaka

Source: Scientific Reports. 11/21/2025, Vol. 15 Issue 1, p1-14. 14p.

Subject Terms: *BIOLUMINESCENCE, *GENE expression, *GENE delivery techniques, *CELL analysis, *CRISPRS, *ARABIDOPSIS

Designing the homogeneous competitive bioluminescence-based assay for tick-borne encephalitis virus (TBEV) point-of-care detection.

Authors: Kudryavtsev, Alexander N. Denisova, Eugenia E. Krasitskaya, Vasilisa V. et al.

Source: Analytical & Bioanalytical Chemistry. Nov2025, Vol. 417 Issue 26, p5931-5939. 9p.

Subject Terms: *TICK-borne encephalitis viruses, *BIOLUMINESCENCE assay, *TICK-borne diseases, *NUCLEIC acid amplification techniques, *RECOMBINANT proteins, *POINT-of-care testing, *IMMUNOASSAY, *ENCEPHALITIS viruses