Výsledky vyhledávání - "Two-Hybrid System Techniques"

Automated FRET Two-Hybrid Analysis.

Autoři: Wei Z Xu Y Wang J a další

Zdroj: Journal of biophotonics [J Biophotonics] 2025 Sep; Vol. 18 (9), pp. e70033. Date of Electronic Publication: 2025 Apr 23.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101318567 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1864-0648 (Electronic) Linking ISSN: 1864063X NLM ISO Abbreviation: J Biophotonics Subsets: MEDLINE

Výrazy ze slovníku MeSH: Fluorescence Resonance Energy Transfer*/methods , Two-Hybrid System Techniques*, Humans ; Automation ; Algorithms ; Image Processing, Computer-Assisted

Bacterial two-hybrid systems evolved: innovations for protein-protein interaction research.

Autoři: Richardson RM Pascal SM

Zdroj: Journal of bacteriology [J Bacteriol] 2025 Aug 21; Vol. 207 (8), pp. e0012925. Date of Electronic Publication: 2025 Aug 01.

Způsob vydávání: Journal Article; Review

Informace o časopise: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 2985120R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5530 (Electronic) Linking ISSN: 00219193 NLM ISO Abbreviation: J Bacteriol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Two-Hybrid System Techniques* , Protein Interaction Mapping*/methods , Bacterial Proteins*/metabolism , Bacterial Proteins*/genetics , Bacteria*/metabolism , Bacteria*/genetics

Liquid Y2H-Seq, a Rapid and Data-Rich Alternative to Conventional Yeast Two-Hybrid Screening.

Autoři: Qi Y Zhang C Yuan S a další

Zdroj: Proteomics [Proteomics] 2025 Jul; Vol. 25 (13), pp. e13970. Date of Electronic Publication: 2025 Jun 08.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101092707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-9861 (Electronic) Linking ISSN: 16159853 NLM ISO Abbreviation: Proteomics Subsets: MEDLINE

Výrazy ze slovníku MeSH: Two-Hybrid System Techniques* , Glycine max*/genetics , Glycine max*/metabolism , Protein Interaction Mapping*/methods, Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Plant Proteins/metabolism ; Plant Proteins/genetics

An E. coli two-hybrid system to investigate human protein-protein interactions.

Autoři: Richardson RM Ho SM Tong L a další

Zdroj: BioTechniques [Biotechniques] 2025 Jul-Aug; Vol. 77 (7-8), pp. 283-295. Date of Electronic Publication: 2025 Aug 20.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Taylor & Francis Country of Publication: England NLM ID: 8306785 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1940-9818 (Electronic) Linking ISSN: 07366205 NLM ISO Abbreviation: Biotechniques Subsets: MEDLINE

Výrazy ze slovníku MeSH: Escherichia coli*/genetics , Escherichia coli*/metabolism , Two-Hybrid System Techniques* , Protein Interaction Mapping*/methods , Heat-Shock Proteins*/metabolism , Heat-Shock Proteins*/genetics, Humans ; Endoplasmic Reticulum Chaperone BiP ; Protein Binding ; Apoptosis Regulatory Proteins/metabolism

Construction of a Membrane Yeast Two-Hybrid Library and Screening of MsPYR1-Like Interacting Proteins in Malus sieversii.

Autoři: Fang Z Zhang K Li J a další

Zdroj: Molecular biotechnology [Mol Biotechnol] 2025 Jun; Vol. 67 (6), pp. 2319-2338. Date of Electronic Publication: 2024 Jun 02.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Springer Country of Publication: Switzerland NLM ID: 9423533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Two-Hybrid System Techniques* , Plant Proteins*/genetics , Plant Proteins*/metabolism , Malus*/genetics , Malus*/metabolism, Gene Library ; Seeds/genetics ; Seeds/metabolism ; Arabidopsis/genetics ; Germination/genetics

Improved constructs for bait RNA display in a bacterial three-hybrid assay.

Autoři: Nguyen LD LeBlanc H Berry KE

Zdroj: Scientific reports [Sci Rep] 2025 Jan 30; Vol. 15 (1), pp. 3820. Date of Electronic Publication: 2025 Jan 30.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Výrazy ze slovníku MeSH: Escherichia coli*/genetics , Escherichia coli*/metabolism , Two-Hybrid System Techniques* , RNA, Bacterial*/genetics , RNA, Bacterial*/metabolism , RNA, Bacterial*/chemistry, Escherichia coli Proteins/metabolism ; Escherichia coli Proteins/genetics ; RNA Folding ; Nucleic Acid Conformation ; Host Factor 1 Protein/metabolism ; Host Factor 1 Protein/genetics ; 5' Untranslated Regions

Pinus radiata cDNA Library for the Screening of Phytophthora Effector Protein Targets in Yeast.

Autoři: Eccersall S Meisrimler CN

Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2892, pp. 169-185.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Gene Library* , Phytophthora*/genetics , Two-Hybrid System Techniques* , Pinus*/parasitology , Pinus*/microbiology , Pinus*/genetics, Plant Diseases/microbiology ; Plant Diseases/parasitology ; Plant Diseases/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Host-Pathogen Interactions/genetics

Probing Pairwise Protein-Protein Interactions with the Bacterial POLAR Assay.

Autoři: Tesseur C Laloux G

Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2953, pp. 103-113.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Protein Interaction Mapping*/methods , Escherichia coli*/metabolism , Escherichia coli*/genetics , Two-Hybrid System Techniques* , Escherichia coli Proteins*/metabolism , Escherichia coli Proteins*/genetics , Bacterial Proteins*/metabolism, Microscopy, Fluorescence/methods ; Protein Binding ; Protein Interaction Maps

Yeast Two-Hybrid Assay for Investigating Antiviral Innate Immunity.

Autoři: Xia S Huo X Zheng C a další

Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2854, pp. 213-220.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Immunity, Innate* , Two-Hybrid System Techniques* , Host-Pathogen Interactions*/immunology, Humans ; Viral Proteins/immunology ; Viral Proteins/metabolism ; Saccharomyces cerevisiae/immunology ; Saccharomyces cerevisiae/genetics ; Protein Binding ; Protein Interaction Mapping/methods

High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology.

Autoři: Xia S Wang J Huo X a další

Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2940, pp. 121-132.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Two-Hybrid System Techniques* , High-Throughput Screening Assays*/methods , Virology*/methods , Viruses*/genetics , Viruses*/metabolism, Humans ; Host-Pathogen Interactions ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Viral Proteins/metabolism ; Viral Proteins/genetics

Expression and function of new candidate regulators of placodal neurogenesis in Xenopus laevis.

Autoři: Hutlet B Schlosser G

Zdroj: Developmental biology [Dev Biol] 2025 Dec; Vol. 528, pp. 128-142. Date of Electronic Publication: 2025 Sep 08.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Elsevier Country of Publication: United States NLM ID: 0372762 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-564X (Electronic) Linking ISSN: 00121606 NLM ISO Abbreviation: Dev Biol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Xenopus laevis*/embryology , Xenopus laevis*/genetics , Xenopus laevis*/metabolism , Neurogenesis*/genetics , Neurogenesis*/physiology , Xenopus Proteins*/metabolism , Xenopus Proteins*/genetics , Gene Expression Regulation, Developmental*, Animals ; Homeodomain Proteins/metabolism ; Homeodomain Proteins/genetics ; Intracellular Signaling Peptides and Proteins/metabolism ; Intracellular Signaling Peptides and Proteins/genetics ; Nuclear Proteins/metabolism ; Nuclear Proteins/genetics ; Protein Tyrosine Phosphatases/metabolism ; Protein Tyrosine Phosphatases/genetics ; Embryo, Nonmammalian/metabolism ; Transcription Factors/metabolism ; Two-Hybrid System Techniques

Protein targeting to Starch 2 and the plastidial phosphorylase 1 revealed protein-protein interactions with photosynthesis proteins in yeast two-hybrid screenings.

Autoři: Muntaha SN Fettke J

Zdroj: Plant signaling & behavior [Plant Signal Behav] 2025 Dec; Vol. 20 (1), pp. 2470775. Date of Electronic Publication: 2025 Feb 26.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101291431 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-2324 (Electronic) Linking ISSN: 15592316 NLM ISO Abbreviation: Plant Signal Behav Subsets: MEDLINE

Výrazy ze slovníku MeSH: Starch*/metabolism , Arabidopsis*/metabolism , Arabidopsis*/genetics , Arabidopsis Proteins*/metabolism , Arabidopsis Proteins*/genetics , Photosynthesis* , Plastids*/metabolism, Two-Hybrid System Techniques ; Protein Binding

The NAC transcription factor CsNAC22 modulates soluble sugar accumulation by activating the expression of sucrose synthase 5 gene in cucumber fruit.

Autoři: Xie Y Liu W Li X a další

Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2025 Nov; Vol. 331 (Pt 2), pp. 148502. Date of Electronic Publication: 2025 Oct 24.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Repressor Proteins*/metabolism , Plant Proteins*/metabolism , Cucumis sativus*/enzymology , Cucumis sativus*/genetics , Glucosyltransferases*/genetics , Gene Expression Regulation, Plant* , Gene Expression Regulation, Enzymologic* , Sucrose*/metabolism, Two-Hybrid System Techniques ; Electrophoretic Mobility Shift Assay ; Fruit/enzymology ; Fruit/genetics

Screening of proteins interacting with infectious hypodermal and hematopoietic necrosis virus in Pacific white shrimp Litopenaeus vannamei.

Autoři: An N Jiang L Du Z a další

Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2025 Nov; Vol. 166, pp. 110618. Date of Electronic Publication: 2025 Aug 05.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Penaeidae*/immunology , Penaeidae*/virology , Penaeidae*/genetics , Arthropod Proteins*/genetics , Arthropod Proteins*/immunology , Arthropod Proteins*/metabolism , Densovirinae*/physiology , Immunity, Innate*/genetics, Animals ; Two-Hybrid System Techniques

Screening of host proteins interacting with the major capsid protein of decapod iridescent virus 1 in Litopenaeus vannamei by the yeast two-hybrid system.

Autoři: Lu Y Tan R Ye J a další

Zdroj: Developmental and comparative immunology [Dev Comp Immunol] 2025 Nov; Vol. 172, pp. 105500. Date of Electronic Publication: 2025 Oct 22.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Penaeidae*/virology , Penaeidae*/immunology , Capsid Proteins*/metabolism , Capsid Proteins*/genetics , DNA Virus Infections*/immunology , Iridovirus*/physiology , Hemocytes*/virology , Arthropod Proteins*/metabolism , Arthropod Proteins*/genetics, Animals ; Two-Hybrid System Techniques ; Host-Pathogen Interactions ; Protein Binding

Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis.

Autoři: Singh D Liu Y Zhu YH a další

Zdroj: ELife [Elife] 2025 Oct 31; Vol. 13. Date of Electronic Publication: 2025 Oct 31.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE

Výrazy ze slovníku MeSH: Cytokinesis* , Schizosaccharomyces*/physiology , Schizosaccharomyces*/metabolism , Schizosaccharomyces*/cytology , Schizosaccharomyces*/genetics , Septins*/metabolism , Septins*/genetics , Exocytosis* , Schizosaccharomyces pombe Proteins*/metabolism , Schizosaccharomyces pombe Proteins*/genetics, Protein Binding ; Two-Hybrid System Techniques ; Cell Membrane/metabolism ; Immunoprecipitation ; Protein Interaction Mapping

Detection of PDR5-mediated alachlor efflux using a chemically induced dimer biosensor.

Autoři: Dubkin H Zimran G Mosquna A

Zdroj: PloS one [PLoS One] 2025 Oct 27; Vol. 20 (10), pp. e0334648. Date of Electronic Publication: 2025 Oct 27 (Print Publication: 2025).

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Výrazy ze slovníku MeSH: Biosensing Techniques*/methods , Acetamides*/metabolism , Saccharomyces cerevisiae Proteins*/metabolism , Saccharomyces cerevisiae Proteins*/genetics , Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae*/genetics , Herbicides*/metabolism , ATP-Binding Cassette Transporters*/metabolism , ATP-Binding Cassette Transporters*/genetics , DNA-Binding Proteins*/metabolism, Two-Hybrid System Techniques

Integrated yeast one-hybrid and molecular docking reveal the binding specificity of FTZ-F1 to GnRHR and Dmrta2 promoters in the scallop, Chlamys farreri.

Autoři: Fu H Fan Q Miao J a další

Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2025 Oct-Dec; Vol. 280, pp. 111149. Date of Electronic Publication: 2025 Aug 23.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Pergamon Country of Publication: England NLM ID: 9516061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1107 (Electronic) Linking ISSN: 10964959 NLM ISO Abbreviation: Comp Biochem Physiol B Biochem Mol Biol Subsets: MEDLINE

Výrazy ze slovníku MeSH: Pectinidae*/metabolism , Pectinidae*/genetics , Molecular Docking Simulation* , Promoter Regions, Genetic* , Receptors, LHRH*/genetics , Receptors, LHRH*/metabolism , Receptors, LHRH*/chemistry , Transcription Factors*/genetics , Transcription Factors*/metabolism , Transcription Factors*/chemistry, Animals ; Two-Hybrid System Techniques ; Protein Binding ; Amino Acid Sequence

Direct connexin-26 interactions with membrane proteins functionally relevant to the cochlea.

Autoři: Leoncio JC Batissoco AC Alegria TGP a další

Zdroj: Human genetics [Hum Genet] 2025 Oct; Vol. 144 (9-10), pp. 983-1000. Date of Electronic Publication: 2025 Aug 13.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: Springer Verlag Country of Publication: Germany NLM ID: 7613873 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1203 (Electronic) Linking ISSN: 03406717 NLM ISO Abbreviation: Hum Genet Subsets: MEDLINE

Výrazy ze slovníku MeSH: Cochlea*/metabolism , Membrane Proteins*/metabolism , Membrane Proteins*/genetics , Connexins*/genetics , Connexins*/metabolism, Humans ; Connexin 26/genetics ; Animals ; Mice ; Deafness/genetics ; Deafness/metabolism ; Two-Hybrid System Techniques

A comprehensive two-hybrid analysis to explore the Legionella pneumophila effector-effector interactome.

Autoři: Mount HO Urbanus ML Sheykhkarimli D a další

Zdroj: MSystems [mSystems] 2024 Dec 17; Vol. 9 (12), pp. e0100424. Date of Electronic Publication: 2024 Nov 11.

Způsob vydávání: Journal Article

Informace o časopise: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5077 (Electronic) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems Subsets: MEDLINE

Výrazy ze slovníku MeSH: Legionella pneumophila*/genetics , Legionella pneumophila*/metabolism , Two-Hybrid System Techniques* , Bacterial Proteins*/metabolism , Bacterial Proteins*/genetics, Humans ; Host-Pathogen Interactions ; Protein Interaction Maps ; Protein Interaction Mapping ; Legionnaires' Disease/microbiology ; Legionnaires' Disease/metabolism ; Type IV Secretion Systems/metabolism ; Type IV Secretion Systems/genetics