Search Results - "Stability"

Effects of buffers on spray-freeze-dried/lyophilized high concentration protein formulations.

Authors: Patil CD Tejasvi Mutukuri T Santosh Arte K et al.

Source: International journal of pharmaceutics [Int J Pharm] 2025 Jan 05; Vol. 668, pp. 124974. Date of Electronic Publication: 2024 Nov 19.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE

MeSH Terms: Freeze Drying* , Serum Albumin, Bovine*/chemistry , Drug Stability*, Buffers ; Mannitol/chemistry ; Trehalose/chemistry ; Hydrogen-Ion Concentration ; Drug Compounding/methods ; Chemistry, Pharmaceutical/methods ; Arginine/chemistry ; X-Ray Diffraction ; Excipients/chemistry ; Protein Stability ; Spectroscopy, Fourier Transform Infrared/methods ; Phosphates/chemistry

"The More, the Better?": The Impact of Sugar-to-Protein Molar Ratio in Freeze-Dried Monoclonal Antibody Formulations on Protein Stability.

Authors: Lo Presti K Jégo M Frieß W

Source: Molecular pharmaceutics [Mol Pharm] 2024 Dec 02; Vol. 21 (12), pp. 6484-6490. Date of Electronic Publication: 2024 Nov 20.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101197791 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-8392 (Electronic) Linking ISSN: 15438384 NLM ISO Abbreviation: Mol Pharm Subsets: MEDLINE

MeSH Terms: Freeze Drying* , Antibodies, Monoclonal*/chemistry , Protein Stability* , Excipients*/chemistry , Sucrose*/chemistry, Chemistry, Pharmaceutical/methods ; Mannitol/chemistry ; Sugars/chemistry ; Drug Stability ; Drug Compounding/methods ; Polysorbates/chemistry ; Proteins/chemistry ; Water/chemistry

Engineered polymeric excipients for enhancing the stability of protein biologics: Poly(N-isopropylacrylamide)-poly(ethylene glycol) (PNIPAM-PEG) block copolymers.

Authors: Jun T Shin SH Won YY

Source: International journal of pharmaceutics [Int J Pharm] 2024 Oct 25; Vol. 664, pp. 124636. Date of Electronic Publication: 2024 Aug 26.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE

MeSH Terms: Polyethylene Glycols*/chemistry , Excipients*/chemistry , Acrylic Resins*/chemistry , Protein Stability*, Drug Stability ; Biological Products/chemistry ; Immunoglobulin G/chemistry ; Polysorbates/chemistry ; Protein Aggregates

Aggregation of therapeutic monoclonal antibodies due to thermal and air/liquid interfacial agitation stress: Occurrence, stability assessment strategies, aggregation mechanism, influencing factors, and ways to enhance stability.

Authors: Sreenivasan S Schöneich C Rathore AS

Source: International journal of pharmaceutics [Int J Pharm] 2024 Dec 05; Vol. 666, pp. 124735. Date of Electronic Publication: 2024 Sep 24.

Publication Type: Journal Article; Review

Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE

MeSH Terms: Antibodies, Monoclonal*/chemistry , Antibodies, Monoclonal*/therapeutic use , Drug Stability* , Protein Aggregates*, Humans ; Protein Stability ; Temperature ; Animals ; Chemistry, Pharmaceutical/methods

The Role of Phase Separation and Local Mobility in the Stabilization of a Lyophilized IgG2 Formulation.

Authors: Lay-Fortenbery A Holcomb RE Henry CS et al.

Source: AAPS PharmSciTech [AAPS PharmSciTech] 2024 Nov 19; Vol. 25 (8), pp. 268. Date of Electronic Publication: 2024 Nov 19.

Publication Type: Journal Article

Journal Info: Publisher: Springer Country of Publication: United States NLM ID: 100960111 Publication Model: Electronic Cited Medium: Internet ISSN: 1530-9932 (Electronic) Linking ISSN: 15309932 NLM ISO Abbreviation: AAPS PharmSciTech Subsets: MEDLINE

MeSH Terms: Immunoglobulin G*/chemistry , Freeze Drying*/methods , Sucrose*/chemistry , Drug Stability* , Chemistry, Pharmaceutical*/methods, Magnetic Resonance Spectroscopy/methods ; Protein Stability ; Excipients/chemistry ; Phase Separation

Role of Charge Heterogeneity on Physical Stability of Monoclonal Antibody Biotherapeutic Products.

Authors: Gupta S Dubey A Rathore AS

Source: Pharmaceutical research [Pharm Res] 2024 Jul; Vol. 41 (7), pp. 1443-1454. Date of Electronic Publication: 2024 Jun 28.

Publication Type: Journal Article

Journal Info: Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 8406521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-904X (Electronic) Linking ISSN: 07248741 NLM ISO Abbreviation: Pharm Res Subsets: MEDLINE

MeSH Terms: Antibodies, Monoclonal*/chemistry , Protein Stability*, Hydrogen-Ion Concentration ; Drug Stability ; Protein Conformation ; Protein Aggregates ; Chromatography, Ion Exchange/methods ; Hydrophobic and Hydrophilic Interactions ; Chromatography, Gel ; Colloids/chemistry ; Biological Products/chemistry ; Humans ; Buffers

Alternative buffer systems in biopharmaceutical formulations and their effect on protein stability.

Authors: Lebar B Zidar M Mravljak J et al.

Source: Acta pharmaceutica (Zagreb, Croatia) [Acta Pharm] 2024 Sep 14; Vol. 74 (3), pp. 479-493. Date of Electronic Publication: 2024 Sep 14 (Print Publication: 2024).

Publication Type: Journal Article

Journal Info: Publisher: Sciendo Country of Publication: Poland NLM ID: 9303678 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1846-9558 (Electronic) Linking ISSN: 13300075 NLM ISO Abbreviation: Acta Pharm Subsets: MEDLINE

MeSH Terms: Protein Stability* , Antibodies, Monoclonal*/chemistry , Excipients*/chemistry, Buffers ; Osmolar Concentration ; Drug Compounding/methods ; Temperature ; Drug Stability ; Histidine/chemistry ; Freezing ; Chemistry, Pharmaceutical/methods ; Arginine/chemistry ; Protein Aggregates

A Thermodynamic Approach to Characterizing Monoclonal Antibody Stability in a Subcutaneous Environment.

Authors: Higdon JR Kang J

Source: Journal of pharmaceutical sciences [J Pharm Sci] 2024 Aug; Vol. 113 (8), pp. 2656-2657. Date of Electronic Publication: 2024 Mar 28.

Publication Type: Letter; Comment

Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 2985195R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6017 (Electronic) Linking ISSN: 00223549 NLM ISO Abbreviation: J Pharm Sci Subsets: MEDLINE

MeSH Terms: Thermodynamics* , Antibodies, Monoclonal*/chemistry , Drug Stability*, Injections, Subcutaneous ; Humans ; Protein Stability

ECT10 is an m ⁶ A reader that negatively regulates the drought stress response in Arabidopsis by modulating mRNA stability.

Authors: Han R Nguyen TKH Yang Z et al.

Source: The Plant journal : for cell and molecular biology [Plant J] 2025 Dec; Vol. 124 (5), pp. e70637.

Publication Type: Journal Article

Journal Info: Publisher: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology Country of Publication: England NLM ID: 9207397 Publication Model: Print Cited Medium: Internet ISSN: 1365-313X (Electronic) Linking ISSN: 09607412 NLM ISO Abbreviation: Plant J Subsets: MEDLINE

MeSH Terms: Arabidopsis*/genetics , Arabidopsis*/physiology , Arabidopsis*/metabolism , Arabidopsis Proteins*/metabolism , Arabidopsis Proteins*/genetics , RNA Stability*/genetics , Adenosine*/metabolism , Adenosine*/analogs & derivatives, Droughts ; Gene Expression Regulation, Plant ; Stress, Physiological/genetics ; RNA, Messenger/metabolism ; RNA, Messenger/genetics

RBM39 silence suppresses esophageal cancer proliferation and metastasis via FANCD2 mRNA destabilization.

Authors: Yang X Li C Zhang C et al.

Source: Cellular signalling [Cell Signal] 2025 Nov; Vol. 135, pp. 112047. Date of Electronic Publication: 2025 Aug 05.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8904683 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3913 (Electronic) Linking ISSN: 08986568 NLM ISO Abbreviation: Cell Signal Subsets: MEDLINE

MeSH Terms: Fanconi Anemia Complementation Group D2 Protein*/genetics , Fanconi Anemia Complementation Group D2 Protein*/metabolism , RNA-Binding Proteins*/genetics , RNA-Binding Proteins*/metabolism , RNA-Binding Proteins*/antagonists & inhibitors , RNA Stability* , Esophageal Neoplasms*/pathology , Esophageal Neoplasms*/genetics , Esophageal Neoplasms*/metabolism, Humans ; Cell Proliferation/genetics ; Animals ; Cell Line, Tumor ; RNA, Messenger/metabolism ; RNA, Messenger/genetics ; Mice ; Neoplasm Metastasis ; Mice, Nude ; Gene Expression Regulation, Neoplastic ; Cell Movement ; 3' Untranslated Regions ; Female

Protein thermostabilization with Protposer: Pushing the stability limits and folding reversibility of a highly-stabilized apoflavodoxin.

Authors: Hidalgo-Toledo A Bazco D Correa-Pérez V et al.

Source: International journal of biological macromolecules [Int J Biol Macromol] 2025 Nov; Vol. 331 (Pt 1), pp. 148333. Date of Electronic Publication: 2025 Oct 15.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE

MeSH Terms: Protein Stability* , Protein Folding* , Apoproteins*/chemistry , Apoproteins*/genetics , Flavodoxin*/chemistry , Flavodoxin*/genetics, Temperature ; Computational Biology/methods ; Hydrogen Bonding ; Mutation ; Protein Denaturation ; Protein Conformation

The P300/ETV4-WDR4 axis promotes colorectal cancer progression via m7G-mediated SPP1 mRNA stabilization and autophagy suppression.

Authors: Deng X Xie R Gong J

Source: Cellular signalling [Cell Signal] 2025 Nov; Vol. 135, pp. 111996. Date of Electronic Publication: 2025 Jul 17.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8904683 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3913 (Electronic) Linking ISSN: 08986568 NLM ISO Abbreviation: Cell Signal Subsets: MEDLINE

MeSH Terms: Colorectal Neoplasms*/pathology , Colorectal Neoplasms*/genetics , Colorectal Neoplasms*/metabolism , Autophagy* , RNA Stability* , Histone-Lysine N-Methyltransferase*/metabolism , Histone-Lysine N-Methyltransferase*/genetics, Humans ; Animals ; Disease Progression ; Mice ; RNA, Messenger/metabolism ; RNA, Messenger/genetics ; Mice, Nude ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Cell Proliferation ; Mice, Inbred BALB C ; Female

Long-Term Stabilization of Dengue Virus RNA at 37 °C for 14 Months Using Silk Fibroin Films.

Authors: Wang N He P Xu B et al.

Source: Viruses [Viruses] 2025 Oct 31; Vol. 17 (11). Date of Electronic Publication: 2025 Oct 31.

Publication Type: Journal Article

Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

MeSH Terms: Dengue Virus*/genetics , RNA, Viral*/chemistry , RNA, Viral*/genetics , Fibroins*/chemistry , RNA Stability*, Temperature ; Dengue/virology ; Dengue/diagnosis ; Humans

The impact of ribosome association on lncRNA stability: a new layer of post-transcriptional control?

Authors: Pienaar CK Towler BP Newbury SF

Source: Biochemical Society transactions [Biochem Soc Trans] 2025 Oct 29; Vol. 53 (5), pp. 1195-1206.

Publication Type: Journal Article; Review

Journal Info: Publisher: Portland Press On The Behalf Of The Biochemical Society Country of Publication: England NLM ID: 7506897 Publication Model: Print Cited Medium: Internet ISSN: 1470-8752 (Electronic) Linking ISSN: 03005127 NLM ISO Abbreviation: Biochem Soc Trans Subsets: MEDLINE

MeSH Terms: RNA, Long Noncoding*/genetics , RNA, Long Noncoding*/metabolism , Ribosomes*/metabolism , Ribosomes*/genetics , RNA Stability*/genetics , RNA Processing, Post-Transcriptional*, Humans ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Animals ; Protein Biosynthesis ; Gene Expression Regulation

The long 5' UTR of nrdAB modulates mRNA levels, stability, and virulence in Pseudomonas aeruginosa PAO1.

Authors: Martínez-Mateos Á Rubio-Canalejas A Pedraz L et al.

Source: Scientific reports [Sci Rep] 2025 Oct 15; Vol. 15 (1), pp. 36012. Date of Electronic Publication: 2025 Oct 15.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH Terms: Pseudomonas aeruginosa*/genetics , Pseudomonas aeruginosa*/pathogenicity , 5' Untranslated Regions*/genetics , RNA, Messenger*/genetics , RNA, Messenger*/metabolism , RNA Stability* , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism , Ribonucleotide Reductases*/genetics , Ribonucleotide Reductases*/metabolism, Virulence/genetics ; Gene Expression Regulation, Bacterial ; Animals ; Pseudomonas Infections/microbiology ; Operon ; Moths/microbiology

The deubiquitylase OTUB1 drives gemcitabine resistance in pancreatic cancer by enhancing pyrimidine metabolism through modulating DHODH mRNA stability.

Authors: Zhang W Liu R Hu J et al.

Source: Cell death & disease [Cell Death Dis] 2025 Oct 06; Vol. 16 (1), pp. 697. Date of Electronic Publication: 2025 Oct 06.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101524092 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-4889 (Electronic) NLM ISO Abbreviation: Cell Death Dis Subsets: MEDLINE

MeSH Terms: Deoxycytidine*/analogs & derivatives , Deoxycytidine*/pharmacology , Deoxycytidine*/therapeutic use , Pancreatic Neoplasms*/genetics , Pancreatic Neoplasms*/drug therapy , Pancreatic Neoplasms*/pathology , Pancreatic Neoplasms*/metabolism , Drug Resistance, Neoplasm*/drug effects , Drug Resistance, Neoplasm*/genetics , Pyrimidines*/metabolism , RNA Stability*/drug effects , RNA Stability*/genetics , Oxidoreductases Acting on CH-CH Group Donors*/genetics , Oxidoreductases Acting on CH-CH Group Donors*/metabolism , Deubiquitinating Enzymes*/metabolism , Deubiquitinating Enzymes*/genetics , DEAD-box RNA Helicases*/metabolism , DEAD-box RNA Helicases*/genetics, Humans ; Gemcitabine ; Animals ; Dihydroorotate Dehydrogenase ; Cell Line, Tumor ; Mice ; Mice, Nude ; RNA, Messenger/metabolism ; RNA, Messenger/genetics

The m ⁶ A writer complex function in main stem apex development by promoting mRNA stability of GIF3, HDA6, and KNAT2/6 in Gossypium hirsutum.

Authors: Huang X Zhang H Liao H et al.

Source: The New phytologist [New Phytol] 2025 Oct; Vol. 248 (2), pp. 721-740. Date of Electronic Publication: 2025 Aug 20.

Publication Type: Journal Article

Journal Info: Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE

MeSH Terms: Gossypium*/genetics , Gossypium*/growth & development , Gossypium*/metabolism , RNA Stability*/genetics , Plant Proteins*/metabolism , Plant Proteins*/genetics , Plant Stems*/growth & development , Plant Stems*/genetics , Adenosine*/analogs & derivatives , Adenosine*/metabolism, Gene Expression Regulation, Plant ; RNA, Messenger/metabolism ; RNA, Messenger/genetics ; Gene Silencing

mRNA Stability: An Unresolved Challenge for Broad Therapeutic Applications.

Authors: Wang Y Wang X Lu Y

Source: Biotechnology journal [Biotechnol J] 2025 Oct; Vol. 20 (10), pp. e70146.

Publication Type: Journal Article; Review

Journal Info: Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 101265833 Publication Model: Print Cited Medium: Internet ISSN: 1860-7314 (Electronic) Linking ISSN: 18606768 NLM ISO Abbreviation: Biotechnol J Subsets: MEDLINE

MeSH Terms: RNA Stability*/genetics , RNA, Messenger*/genetics , RNA, Messenger*/chemistry , RNA, Messenger*/therapeutic use , RNA, Messenger*/metabolism, Humans ; Biotechnology/methods ; Animals ; 5' Untranslated Regions ; 3' Untranslated Regions

The natural statin α,β-dehydromonacolin K exerts anti-secretory effect in human intestinal epithelial cells via a nonsense-mediated mRNA decay-dependent mechanism.

Authors: Satitsri S Khumjiang R Tansakul C et al.

Source: Pharmaceutical biology [Pharm Biol] 2025 Dec; Vol. 63 (1), pp. 645-662. Date of Electronic Publication: 2025 Aug 22.

Publication Type: Journal Article

Journal Info: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE

MeSH Terms: Intestinal Mucosa*/drug effects , Intestinal Mucosa*/metabolism , Lovastatin*/pharmacology , Lovastatin*/analogs & derivatives , Epithelial Cells*/drug effects , Epithelial Cells*/metabolism , RNA Stability*/drug effects , Hydroxymethylglutaryl-CoA Reductase Inhibitors*/pharmacology, Humans ; Chlorides/metabolism ; Cyclic AMP/metabolism ; Cystic Fibrosis Transmembrane Conductance Regulator/metabolism ; Proteomics ; RNA, Messenger/metabolism ; Diarrhea/drug therapy

PCBP2 Promotes NRG4 mRNA Stability to Diminish Angiotensin II-Induced Hypertrophy, NLRP3 Inflammasome Activation, and Oxidative Stress of AC16 Cardiomyocytes.

Authors: Zhang Y Liu Y Wang Y

Source: Cell biochemistry and biophysics [Cell Biochem Biophys] 2025 Dec; Vol. 83 (4), pp. 4989-5001. Date of Electronic Publication: 2025 Jul 18.

Publication Type: Journal Article

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9701934 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0283 (Electronic) Linking ISSN: 10859195 NLM ISO Abbreviation: Cell Biochem Biophys Subsets: MEDLINE

MeSH Terms: Oxidative Stress*/drug effects , Angiotensin II*/pharmacology , RNA-Binding Proteins*/metabolism , RNA-Binding Proteins*/genetics , Myocytes, Cardiac*/metabolism , Myocytes, Cardiac*/drug effects , Myocytes, Cardiac*/cytology , Myocytes, Cardiac*/pathology , NLR Family, Pyrin Domain-Containing 3 Protein*/metabolism , RNA Stability*/drug effects , Inflammasomes*/metabolism , Cardiomegaly*/metabolism , Cardiomegaly*/pathology , Cardiomegaly*/chemically induced, Animals ; Cell Line ; Mice ; TOR Serine-Threonine Kinases/metabolism ; Signal Transduction/drug effects ; RNA, Messenger/metabolism ; RNA, Messenger/genetics