Search Results - "Single-Cell Analysis"

Deciphering cellular heterogeneity: Breakthroughs and prospects of single-cell-level SERS analysis in precision medicine.

Authors: Chen B Gao J Sun H et al.

Source: Methods (San Diego, Calif.) [Methods] 2025 Dec; Vol. 244, pp. 7-29. Date of Electronic Publication: 2025 Sep 07.

Publication Type: Journal Article; Review

Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/methods , Single-Cell Analysis*/instrumentation , Spectrum Analysis, Raman*/methods , Spectrum Analysis, Raman*/instrumentation , Precision Medicine*/methods , Neoplasms*/pathology , Neoplasms*/diagnosis, Humans ; Neoplastic Cells, Circulating/pathology ; Tumor Microenvironment ; Metabolomics/methods

Fiber-Tip Shear Force Probe for Single-Cell Adhesion Force Measurements.

Authors: Zou M Chen Y Liu Y et al.

Source: ACS sensors [ACS Sens] 2025 Nov 28; Vol. 10 (11), pp. 8874-8884. Date of Electronic Publication: 2025 Nov 18.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE

MeSH Terms: Cell Adhesion* , Single-Cell Analysis*/instrumentation , Single-Cell Analysis*/methods , Microscopy, Atomic Force*/instrumentation , Microscopy, Atomic Force*/methods, Humans ; MCF-7 Cells

Spatial-Temporal Multiomics Profiling of Living Single Cells on Chip for Cancer Therapy.

Authors: Yu K Chen S Zhu R

Source: ACS sensors [ACS Sens] 2025 Oct 24; Vol. 10 (10), pp. 7497-7507. Date of Electronic Publication: 2025 Oct 12.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/methods , Single-Cell Analysis*/instrumentation , Lab-On-A-Chip Devices* , Liver Neoplasms*/therapy , Liver Neoplasms*/pathology , Liver Neoplasms*/metabolism, Humans ; Spectrum Analysis, Raman ; Cell Line, Tumor ; Antineoplastic Agents/pharmacology ; Multiomics

Dynamic monitoring of Cytochrome c in single tumor cell for anticancer drug susceptibility assessment.

Authors: Long L Zhou H Tan H et al.

Source: Analytica chimica acta [Anal Chim Acta] 2025 Dec 22; Vol. 1380, pp. 344763. Date of Electronic Publication: 2025 Oct 13.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE

MeSH Terms: Cytochromes c*/analysis , Cytochromes c*/metabolism , Antineoplastic Agents*/pharmacology , Antineoplastic Agents*/chemistry , Biosensing Techniques* , Single-Cell Analysis*, Humans ; HeLa Cells ; Aptamers, Nucleotide/chemistry ; Electrochemical Techniques ; Cisplatin/pharmacology ; Cell Survival/drug effects ; Platinum/chemistry ; Drug Screening Assays, Antitumor ; Apoptosis/drug effects ; Doxorubicin/pharmacology ; Electrodes

Single-cell multi-omics characterize colorectal tumors, adjacent healthy tissue and matched (tumor) organoids identifying CRC-unique features.

Authors: Yu Z Derksen M Te Pas BM et al.

Source: International journal of cancer [Int J Cancer] 2025 Dec 15; Vol. 157 (12), pp. 2619-2634. Date of Electronic Publication: 2025 Aug 23.

Publication Type: Journal Article

Journal Info: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0042124 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0215 (Electronic) Linking ISSN: 00207136 NLM ISO Abbreviation: Int J Cancer Subsets: MEDLINE

MeSH Terms: Organoids*/pathology , Organoids*/metabolism , Colorectal Neoplasms*/genetics , Colorectal Neoplasms*/pathology , Single-Cell Analysis*/methods, Humans ; Mutation ; Tumor Microenvironment/genetics ; Transcriptome ; Multiomics

Integrating Multiple Clustering Techniques and Performance Measures via Ranking for scRNA-Seq Data.

Authors: Visser O Datta S

Source: Statistics in medicine [Stat Med] 2025 Dec; Vol. 44 (28-30), pp. e70331.

Publication Type: Journal Article

Journal Info: Publisher: Wiley Country of Publication: England NLM ID: 8215016 Publication Model: Print Cited Medium: Internet ISSN: 1097-0258 (Electronic) Linking ISSN: 02776715 NLM ISO Abbreviation: Stat Med Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/methods , Single-Cell Analysis*/statistics & numerical data , RNA-Seq*/methods , RNA-Seq*/statistics & numerical data, Cluster Analysis ; Humans ; Gene Expression Profiling/methods ; Single-Cell Gene Expression Analysis

D3Impute: Dropout-aware discrimination, distribution-aware modeling, and density-guide imputation for scRNA-seq data.

Authors: Huang S Jiang L Yi M et al.

Source: PLoS computational biology [PLoS Comput Biol] 2025 Dec 01; Vol. 21 (12), pp. e1013744. Date of Electronic Publication: 2025 Dec 01 (Print Publication: 2025).

Publication Type: Journal Article

Journal Info: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238922 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7358 (Electronic) Linking ISSN: 1553734X NLM ISO Abbreviation: PLoS Comput Biol Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/methods , Single-Cell Analysis*/statistics & numerical data , Sequence Analysis, RNA*/methods , RNA-Seq*/methods, Humans ; Computational Biology/methods ; Algorithms ; Software ; Gene Expression Profiling/methods ; Transcriptome/genetics ; Single-Cell Gene Expression Analysis

Scaling Effects on Single-Cell Manipulation Using Magnetic Forces at Edge of Flat Plate With Elliptically Micro-Projection.

Authors: Ota S Yasuga H Akagawa T et al.

Source: Biotechnology and bioengineering [Biotechnol Bioeng] 2025 Dec; Vol. 122 (12), pp. 3355-3365. Date of Electronic Publication: 2025 Sep 02.

Publication Type: Journal Article

Journal Info: Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0290 (Electronic) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/methods , Single-Cell Analysis*/instrumentation , Micromanipulation*/methods , Micromanipulation*/instrumentation, Magnetic Fields ; Magnetite Nanoparticles/chemistry ; Equipment Design ; Microtechnology ; Animals

Single-cell microbiota phenotyping reveals distinct disease and therapy-associated signatures in Crohn's disease.

Authors: Budzinski L Kang GU Riedel R et al.

Source: Gut microbes [Gut Microbes] 2025 Dec; Vol. 17 (1), pp. 2452250. Date of Electronic Publication: 2025 Jan 15.

Publication Type: Journal Article

Journal Info: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101495343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1949-0984 (Electronic) Linking ISSN: 19490976 NLM ISO Abbreviation: Gut Microbes Subsets: MEDLINE

MeSH Terms: Crohn Disease*/microbiology , Crohn Disease*/drug therapy , Gastrointestinal Microbiome* , Single-Cell Analysis*/methods , Bacteria*/classification , Bacteria*/genetics , Bacteria*/isolation & purification, Humans ; Male ; Female ; Adult ; Phenotype ; Flow Cytometry ; Immunoglobulin A/metabolism ; Middle Aged ; Dysbiosis/microbiology ; Case-Control Studies ; Young Adult ; Feces/microbiology

Single-Cell Insights into Unicystic and Solid Ameloblastoma Heterogeneity.

Authors: Feng C Dou X Li SR et al.

Source: Journal of dental research [J Dent Res] 2025 Dec; Vol. 104 (13), pp. 1506-1516. Date of Electronic Publication: 2025 Jun 26.

Publication Type: Journal Article

Journal Info: Publisher: Sage Country of Publication: United States NLM ID: 0354343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1544-0591 (Electronic) Linking ISSN: 00220345 NLM ISO Abbreviation: J Dent Res Subsets: MEDLINE

MeSH Terms: Ameloblastoma*/pathology , Ameloblastoma*/genetics , Ameloblastoma*/metabolism , Ameloblastoma*/classification , Single-Cell Analysis* , Jaw Neoplasms*/pathology , Jaw Neoplasms*/genetics , Jaw Neoplasms*/metabolism, Humans ; Macrophages/metabolism ; RANK Ligand/metabolism ; Epithelial Cells/pathology ; Osteoclasts

Robotic manipulations of single cells using a large-volume piezoelectric micropipette with nanoliter precision.

Authors: Kovacs B Novak S Sallai I et al.

Source: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2025 Dec; Vol. 256 (Pt 1), pp. 114972. Date of Electronic Publication: 2025 Jul 22.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/instrumentation , Single-Cell Analysis*/methods , Robotics*/instrumentation , Robotics*/methods , Nanotechnology*/instrumentation, Humans ; HeLa Cells ; Cell Adhesion ; Oligopeptides/chemistry

Advances in microfluidic chip technology for cell analysis.

Authors: Shu L Zhang Y

Source: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2025 Dec; Vol. 41 (12), pp. 1829-1851. Date of Electronic Publication: 2025 Sep 30.

Publication Type: Journal Article; Review

Journal Info: Publisher: Springer Country of Publication: Switzerland NLM ID: 8511078 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1348-2246 (Electronic) Linking ISSN: 09106340 NLM ISO Abbreviation: Anal Sci Subsets: MEDLINE

MeSH Terms: Lab-On-A-Chip Devices* , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods , Single-Cell Analysis*/instrumentation, Humans ; Animals ; Cell Separation/instrumentation

Single particle and single cell analysis by ICP-MS with pneumatic nebulisation: Comparing sample introduction systems.

Authors: Suárez Priede A Corte-Rodríguez M Gödde H et al.

Source: Talanta [Talanta] 2025 Dec 01; Vol. 295, pp. 128372. Date of Electronic Publication: 2025 May 22.

Publication Type: Journal Article; Comparative Study

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE

MeSH Terms: Mass Spectrometry*/methods , Mass Spectrometry*/instrumentation , Nebulizers and Vaporizers* , Single-Cell Analysis*/methods , Single-Cell Analysis*/instrumentation , Metal Nanoparticles*/chemistry, Gold/chemistry ; Saccharomyces cerevisiae/chemistry ; Saccharomyces cerevisiae/cytology ; Europium/chemistry ; Particle Size ; Polystyrenes/chemistry

On-chip single-cell mass spectrometry for streamlined metabolic exploration enabled by a reversibly-bonded microfluidic platform.

Authors: You J Chen A Qiu J et al.

Source: Analytica chimica acta [Anal Chim Acta] 2025 Nov 22; Vol. 1376, pp. 344608. Date of Electronic Publication: 2025 Sep 03.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/instrumentation , Single-Cell Analysis*/methods , Mass Spectrometry* , Lab-On-A-Chip Devices* , Microfluidic Analytical Techniques*/instrumentation, Humans

A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants.

Authors: Ueno R Ito S Oyama T

Source: Scientific reports [Sci Rep] 2025 Nov 21; Vol. 15 (1), pp. 41271. Date of Electronic Publication: 2025 Nov 21.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH Terms: CRISPR-Cas Systems*/genetics , Arabidopsis*/genetics , Arabidopsis*/metabolism , Single-Cell Analysis*/methods , Genes, Reporter* , Luminescent Measurements*/methods , Gene Expression Regulation, Plant*, Plants, Genetically Modified/genetics ; Luciferases/genetics ; Luciferases/metabolism ; Single-Cell Gene Expression Analysis

Research Progress Regarding the Use of Single-Cell Sequencing Technology in Analyzing Tumor Endothelial Cell Pathophysiology.

Authors: Zhao S Liu S Shao W et al.

Source: International journal of molecular sciences [Int J Mol Sci] 2025 Nov 18; Vol. 26 (22). Date of Electronic Publication: 2025 Nov 18.

Publication Type: Journal Article; Review

Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/methods , Endothelial Cells*/pathology , Endothelial Cells*/metabolism , Neoplasms*/genetics , Neoplasms*/pathology , Neoplasms*/metabolism, Humans ; Tumor Microenvironment/genetics ; Animals ; Neovascularization, Pathologic/genetics ; Neovascularization, Pathologic/pathology

A Simple and Flexible Strategy for Single-Cell Proteomic Analysis Based on Protein Immobilization and Digestion Tube Reactor.

Authors: Zhang W Weng L Yan G et al.

Source: Analytical chemistry [Anal Chem] 2025 Nov 18; Vol. 97 (45), pp. 25245-25256. Date of Electronic Publication: 2025 Nov 04.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/methods , Single-Cell Analysis*/instrumentation , Proteomics*/methods , Immobilized Proteins*/chemistry , Immobilized Proteins*/metabolism , Proteins*/analysis , Proteins*/metabolism, Humans ; Tandem Mass Spectrometry ; Chromatography, Liquid ; A549 Cells

Transforming microfluidics for single-cell analysis with robotics and artificial intelligence.

Authors: Cheng J Anne R Chen YC

Source: Lab on a chip [Lab Chip] 2025 Nov 18; Vol. 25 (23), pp. 6100-6125. Date of Electronic Publication: 2025 Nov 18.

Publication Type: Journal Article; Review

Journal Info: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101128948 Publication Model: Electronic Cited Medium: Internet ISSN: 1473-0189 (Electronic) Linking ISSN: 14730189 NLM ISO Abbreviation: Lab Chip Subsets: MEDLINE

MeSH Terms: Single-Cell Analysis*/instrumentation , Single-Cell Analysis*/methods , Robotics* , Artificial Intelligence* , Microfluidic Analytical Techniques*/instrumentation , Microfluidics*, Humans ; Lab-On-A-Chip Devices ; Animals ; Deep Learning

In situ spatial profiling of cytochrome c release at the single-cell level under photothermal stress utilizing a 3D bifunctional SERS substrate.

Authors: Lu Y Shi DW Pan SF et al.

Source: Biosensors & bioelectronics [Biosens Bioelectron] 2025 Nov 15; Vol. 288, pp. 117818. Date of Electronic Publication: 2025 Jul 30.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE

MeSH Terms: Cytochromes c*/metabolism , Cytochromes c*/isolation & purification , Cytochromes c*/analysis , Spectrum Analysis, Raman*/methods , Single-Cell Analysis*/methods , Biosensing Techniques*/methods, Humans ; Apoptosis ; Nanostructures/chemistry ; Gold/chemistry ; Metal Nanoparticles/chemistry ; Cell Line, Tumor ; Mitochondria ; Animals

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology.

Authors: Liu Z Tian Y Zhang Z et al.

Source: Journal of visualized experiments : JoVE [J Vis Exp] 2025 Nov 14 (225). Date of Electronic Publication: 2025 Nov 14.

Publication Type: Journal Article; Video-Audio Media

Journal Info: Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE

MeSH Terms: Neoplastic Cells, Circulating*/pathology , Single-Cell Analysis*/methods , Single-Cell Analysis*/instrumentation , Microfluidic Analytical Techniques*/methods , Microfluidic Analytical Techniques*/instrumentation , Cell Separation*/methods , Cell Separation*/instrumentation , Sequence Analysis, RNA*/methods, Humans ; High-Throughput Nucleotide Sequencing/methods