Suchergebnisse - "Reverse genetics"

First Versatile Reverse Genetics System for DNA Viruses Using Circular Polymerase Extension Reaction.

Autoren: Yamamoto H Tamura T Suzuki R et al.

Quelle: Microbiology and immunology [Microbiol Immunol] 2025 Dec; Vol. 69 (12), pp. 619-622. Date of Electronic Publication: 2025 Oct 12.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Wiley-Blackwell Country of Publication: Australia NLM ID: 7703966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1348-0421 (Electronic) Linking ISSN: 03855600 NLM ISO Abbreviation: Microbiol Immunol Subsets: MEDLINE

MeSH-Schlagworte: Reverse Genetics*/methods , Adenoviridae*/genetics , DNA Viruses*/genetics, Humans ; Virus Replication ; DNA, Viral/genetics

Development of the coronavirus reverse genetic system: Core technology for pathogenesis mechanisms research and vaccine/drug development.

Autoren: Zhang Y Hou R Wei Z et al.

Quelle: Virulence [Virulence] 2025 Dec; Vol. 16 (1), pp. 2525930. Date of Electronic Publication: 2025 Jun 28.

Publikationsart: Journal Article; Review; Research Support, Non-U.S. Gov't

Info zur Zeitschrift: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101531386 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-5608 (Electronic) Linking ISSN: 21505594 NLM ISO Abbreviation: Virulence Subsets: MEDLINE

MeSH-Schlagworte: Reverse Genetics*/methods , Vaccine Development* , Coronavirus*/genetics , Coronavirus*/pathogenicity, Humans ; Animals ; Drug Development ; SARS-CoV-2/genetics ; SARS-CoV-2/pathogenicity ; Viral Vaccines/immunology ; Antiviral Agents/pharmacology ; COVID-19 Vaccines

The ITR regions play a significant role in modulating viral pathogenicity demonstrated by the reverse genetics system of a novel goose parvovirus.

Autoren: Xue Y Liu M Ji L et al.

Quelle: Veterinary microbiology [Vet Microbiol] 2025 Dec; Vol. 311, pp. 110746. Date of Electronic Publication: 2025 Oct 08.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE

MeSH-Schlagworte: Parvoviridae Infections*/veterinary , Parvoviridae Infections*/virology , Reverse Genetics* , Poultry Diseases*/virology , Geese*/virology , Parvovirinae*/pathogenicity , Parvovirinae*/genetics , Parvovirus*/genetics , Parvovirus*/pathogenicity, Animals ; Ducks/virology ; Virulence/genetics ; Genome, Viral/genetics

SCR Organism: Avian dependoparvovirus 1

Positive-negative Selection: The counterselection breakthrough that conventionalized reverse genetics in the mouse.

Autoren: Letsou A; Department of Human Genetics, Spencer Fox Eccles School of Medicine, University of Utah, Salt Lake City, UT, 84112, USA. Electronic address: aletsou@genetics.utah.edu.

Quelle: Developmental biology [Dev Biol] 2025 Jul; Vol. 523, pp. 139-143. Date of Electronic Publication: 2025 Apr 19.

Publikationsart: Historical Article; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Review

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 0372762 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-564X (Electronic) Linking ISSN: 00121606 NLM ISO Abbreviation: Dev Biol Subsets: MEDLINE

MeSH-Schlagworte: Developmental Biology*/history , Reverse Genetics*/methods , Reverse Genetics*/history , Selection, Genetic*, Animals ; Humans ; Mice ; History, 20th Century ; History, 21st Century ; Proto-Oncogene Mas

De novo generation of viruses in animals: from infection models to vaccine development.

Autoren: Cochin M Driouich J-S Luciani L et al.

Quelle: MSphere [mSphere] 2025 Sep 30; Vol. 10 (9), pp. e0074224. Date of Electronic Publication: 2025 Aug 11.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101674533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5042 (Electronic) Linking ISSN: 23795042 NLM ISO Abbreviation: mSphere Subsets: MEDLINE

MeSH-Schlagworte: Vaccine Development*/methods , Reverse Genetics*/methods , Viruses*/genetics , Viral Vaccines*/immunology , Viral Vaccines*/genetics , Virus Diseases*/prevention & control , Virus Diseases*/virology, Animals ; Vaccines, Attenuated/immunology ; Vaccines, Attenuated/genetics ; Disease Models, Animal ; Vaccines, DNA/immunology

Reverse genetics system for Tick-borne encephalitis virus using Circular Polymerase Extension Reaction.

Autoren: Mitsunaga S Tamura T Nyampong S et al.

Quelle: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2025 Aug; Vol. 132, pp. 105776. Date of Electronic Publication: 2025 Jun 01.

Publikationsart: Journal Article; Research Support, Non-U.S. Gov't

Info zur Zeitschrift: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101084138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-7257 (Electronic) Linking ISSN: 15671348 NLM ISO Abbreviation: Infect Genet Evol Subsets: MEDLINE

MeSH-Schlagworte: Encephalitis Viruses, Tick-Borne*/genetics , Encephalitis Viruses, Tick-Borne*/pathogenicity , Reverse Genetics*/methods , Encephalitis, Tick-Borne*/virology , Polymerase Chain Reaction*/methods, Animals ; Mice ; Genome, Viral ; Cell Line ; Mice, Inbred C57BL ; Cricetinae ; Virulence

Rapid generation of HCoV-229E and HCoV-OC43 reporter viruses and replicons for antiviral research.

Autoren: Zhang Y Chen J Feng H et al.

Quelle: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2025 Jul 22; Vol. 15, pp. 1614369. Date of Electronic Publication: 2025 Jul 22 (Print Publication: 2025).

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE

MeSH-Schlagworte: Antiviral Agents*/pharmacology , Genes, Reporter* , Replicon*/genetics , Coronavirus 229E, Human*/genetics , Coronavirus 229E, Human*/drug effects , Coronavirus OC43, Human*/genetics , Coronavirus OC43, Human*/drug effects , Reverse Genetics*/methods, Humans ; CRISPR-Cas Systems ; Luciferases/genetics ; Recombination, Genetic ; Cloning, Molecular ; Saccharomyces cerevisiae/genetics ; Animals ; Adenosine Monophosphate/analogs & derivatives ; Alanine/analogs & derivatives

Reverse genetics rescue of sylvatic dengue viruses.

Autoren: Gendler P Barbachano-Guerrero A Chappell SD et al.

Quelle: Journal of virology [J Virol] 2025 Jul 22; Vol. 99 (7), pp. e0045025. Date of Electronic Publication: 2025 Jun 04.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE

MeSH-Schlagworte: Dengue Virus*/genetics , Dengue Virus*/physiology , Dengue Virus*/growth & development , Reverse Genetics*/methods , Dengue*/virology, Animals ; Humans ; Virus Replication ; Genome, Viral ; Cell Line ; Aedes/virology ; Culicidae/virology

Rapid reconstruction of infectious bronchitis virus expressing fluorescent protein from its nsp2 gene based on transformation-associated recombination platform.

Autoren: Li Y Duan L Tang L et al.

Quelle: Journal of virology [J Virol] 2025 Jul 22; Vol. 99 (7), pp. e0053525. Date of Electronic Publication: 2025 Jun 05.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE

MeSH-Schlagworte: Infectious bronchitis virus*/genetics , Infectious bronchitis virus*/pathogenicity , Recombination, Genetic* , Reverse Genetics*/methods , Viral Nonstructural Proteins*/genetics , Luminescent Proteins*/genetics , Luminescent Proteins*/metabolism, Animals ; Chick Embryo ; Genome, Viral ; Escherichia coli/genetics ; Transformation, Genetic ; Virus Replication ; Saccharomyces cerevisiae/genetics ; Coronavirus Infections/virology ; Chickens

Optimization of an infectious subgenomic amplicons reverse genetics protocol for the rescue of synthetic coronaviruses.

Autoren: Puglia I Caporale M Di Teodoro G et al.

Quelle: Journal of virological methods [J Virol Methods] 2025 Jul; Vol. 336, pp. 115152. Date of Electronic Publication: 2025 Apr 04.

Publikationsart: Journal Article; Research Support, Non-U.S. Gov't

Info zur Zeitschrift: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

MeSH-Schlagworte: Reverse Genetics*/methods , SARS-CoV-2*/genetics , SARS-CoV-2*/pathogenicity , Genome, Viral*, Animals ; Mice ; COVID-19/virology ; Chlorocebus aethiops ; Vero Cells ; Humans ; RNA, Viral/genetics

Efficient and modular reverse genetics system for rapid generation of recombinant severe acute respiratory syndrome coronavirus 2.

Autoren: Bae S Myoung J

Quelle: Journal of microbiology (Seoul, Korea) [J Microbiol] 2025 Jul; Vol. 63 (7), pp. e2504015. Date of Electronic Publication: 2025 Jul 21.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Microbiological Society Of Korea Country of Publication: Korea (South) NLM ID: 9703165 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1976-3794 (Electronic) Linking ISSN: 12258873 NLM ISO Abbreviation: J Microbiol Subsets: MEDLINE

MeSH-Schlagworte: Reverse Genetics*/methods , SARS-CoV-2*/genetics, Humans ; Genome, Viral ; Animals ; Chlorocebus aethiops ; Vero Cells ; COVID-19/virology ; Plasmids/genetics ; DNA, Complementary/genetics ; Cloning, Molecular

Serial viral passage and reverse genetics protocol for identifying the antiviral drug targets of EV-D68 antivirals.

Autoren: Tan H Li K Wang J

Quelle: STAR protocols [STAR Protoc] 2025 Sep 19; Vol. 6 (3), pp. 104053. Date of Electronic Publication: 2025 Aug 29.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE

MeSH-Schlagworte: Antiviral Agents*/pharmacology , Reverse Genetics*/methods, Humans ; Genome, Viral/genetics

Use of Maize ( Zea mays L.) Mutator Transposon-Induced Mutants of the BonnMu Resource for Forward and Reverse Genetics Studies.

Autoren: Win YN Pöschel M Stöcker T et al.

Quelle: Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2025 Sep 02; Vol. 2025 (9), pp. pdb.prot108587. Date of Electronic Publication: 2025 Sep 02.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 101524530 Publication Model: Electronic Cited Medium: Internet ISSN: 1559-6095 (Electronic) Linking ISSN: 15596095 NLM ISO Abbreviation: Cold Spring Harb Protoc Subsets: MEDLINE

MeSH-Schlagworte: Zea mays*/genetics , DNA Transposable Elements* , Reverse Genetics*/methods , Mutation* , Mutagenesis, Insertional*, Phenotype

Growth properties of recombinant equine influenza viruses with different backbones generated by reverse genetics in embryonated chicken eggs.

Autoren: Nemoto M Kawanishi N Kambayashi Y et al.

Quelle: Archives of virology [Arch Virol] 2025 Jul 12; Vol. 170 (8), pp. 181. Date of Electronic Publication: 2025 Jul 12.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-8798 (Electronic) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE

MeSH-Schlagworte: Reverse Genetics*/methods , Influenza A Virus, H3N8 Subtype*/growth & development , Influenza A Virus, H3N8 Subtype*/genetics, Animals ; Horses ; Chick Embryo ; Influenza Vaccines/genetics

Establishment of a reverse genetics system for studying human immune functions in mice.

Autoren: Pal P Gao S Gao H et al.

Quelle: Science advances [Sci Adv] 2025 Jul 11; Vol. 11 (28), pp. eadu1561. Date of Electronic Publication: 2025 Jul 09.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE

MeSH-Schlagworte: Reverse Genetics*/methods, Animals ; Humans ; Mice ; Antigens, CD34/metabolism ; Antigens, CD34/genetics ; CRISPR-Cas Systems ; Gene Editing ; Fetal Blood/cytology ; Disease Models, Animal

Plasmid-Based Reverse Genetics System Enabling One-Step Generation of Genotype 3 Hepatitis E Virus.

Autoren: Kobayashi T Nishiyama T Yamada K et al.

Quelle: Viruses [Viruses] 2025 May 03; Vol. 17 (5). Date of Electronic Publication: 2025 May 03.

Publikationsart: Journal Article; Research Support, Non-U.S. Gov't

Info zur Zeitschrift: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

MeSH-Schlagworte: Hepatitis E virus*/genetics , Hepatitis E virus*/physiology , Hepatitis E virus*/classification , Plasmids*/genetics , Reverse Genetics*/methods, Virus Replication ; Humans ; Genotype ; Genome, Viral ; RNA, Viral/genetics ; Promoter Regions, Genetic ; Hepatitis Delta Virus/genetics ; Cell Line

Plasmid DNA-based reverse genetics as a platform for manufacturing of bluetongue vaccine.

Autoren: Mlingo TAM Theron J Mokoena NB

Quelle: Journal of virology [J Virol] 2025 Apr 15; Vol. 99 (4), pp. e0013925. Date of Electronic Publication: 2025 Mar 06.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE

MeSH-Schlagworte: Bluetongue virus*/immunology , Bluetongue virus*/genetics , Viral Vaccines*/immunology , Viral Vaccines*/genetics , Plasmids*/genetics , Plasmids*/immunology , Bluetongue*/prevention & control , Bluetongue*/immunology , Bluetongue*/virology , Reverse Genetics*/methods , Vaccines, DNA*/immunology , Vaccines, DNA*/genetics, Animals ; Sheep ; Antibodies, Viral/immunology ; Antibodies, Viral/blood ; Antibodies, Neutralizing/immunology ; Antibodies, Neutralizing/blood ; Capsid Proteins/immunology ; Capsid Proteins/genetics ; Serogroup ; Vaccines, Attenuated/immunology ; Vaccines, Synthetic/immunology ; Vaccines, Synthetic/genetics ; Immunogenicity, Vaccine

Development of a reverse genetics system for Getah virus and characterization of rescued strains.

Autoren: Cai R He Q Wang Q et al.

Quelle: Veterinary research [Vet Res] 2025 Apr 12; Vol. 56 (1), pp. 80. Date of Electronic Publication: 2025 Apr 12.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE

MeSH-Schlagworte: Reverse Genetics*/methods , Alphavirus*/genetics , Alphavirus*/physiology, Animals ; Genome, Viral

Application of versatile reverse genetics system for feline coronavirus.

Autoren: Kida I Tamura T Kuroda Y et al.

Quelle: Microbiology spectrum [Microbiol Spectr] 2025 Apr; Vol. 13 (4), pp. e0269224. Date of Electronic Publication: 2025 Mar 10.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE

MeSH-Schlagworte: Coronavirus, Feline*/genetics , Coronavirus, Feline*/growth & development , Coronavirus, Feline*/drug effects , Reverse Genetics*/methods , Feline Infectious Peritonitis*/virology, Animals ; Cats ; Spike Glycoprotein, Coronavirus/genetics ; Cell Line ; Virus Replication ; Genes, Reporter ; Genome, Viral ; Antiviral Agents/pharmacology

Application of reverse genetics system to Chikungunya virus study.

Autoren: Suzuki Y; Department of Microbiology and Infection Control, Faculty of Medicine, Osaka Medical and Pharmaceutical University, Takatsuki, Japan. Electronic address: youichi.suzuki@ompu.ac.jp.

Quelle: Virology [Virology] 2025 Apr; Vol. 605, pp. 110465. Date of Electronic Publication: 2025 Feb 27.

Publikationsart: Journal Article; Review; Research Support, Non-U.S. Gov't

Info zur Zeitschrift: Publisher: Academic Press Country of Publication: United States NLM ID: 0110674 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0341 (Electronic) Linking ISSN: 00426822 NLM ISO Abbreviation: Virology Subsets: MEDLINE

MeSH-Schlagworte: Chikungunya virus*/genetics , Chikungunya virus*/physiology , Chikungunya virus*/pathogenicity , Reverse Genetics*/methods , Chikungunya Fever*/virology , Chikungunya Fever*/prevention & control, Humans ; Animals ; Genome, Viral ; Virus Replication