Search Results - "Protein Engineering/methods"

Integrating genetic algorithms and language models for enhanced enzyme design

Authors: Nana Teukam, Yves Gaetan Zipoli, Federico Laino, Teodoro et al.

Source: Nana Teukam, Y G, Zipoli, F, Laino, T, Criscuolo, E, Grisoni, F & Manica, M 2025, 'Integrating genetic algorithms and language models for enhanced enzyme design', Briefings in Bioinformatics, vol. 26, no. 1, bbae675. https://doi.org/10.1093/bib/bbae675

Subject Terms: biocatalysis, computational protein design, enzyme optimization, genetic algorithms, large language models, Algorithms, Protein Engineering/methods, Computational Biology/methods, Enzymes/chemistry

File Description: application/pdf

Relation: info:eu-repo/semantics/altIdentifier/pmid/39780486; info:eu-repo/semantics/altIdentifier/pissn/1467-5463

Availability: https://research.tue.nl/en/publications/fbb3a752-0d25-4d4f-af0c-e5aaf63d3173
https://doi.org/10.1093/bib/bbae675
https://pure.tue.nl/ws/files/351369366/bbae675.pdf
https://www.scopus.com/pages/publications/85215090523

Backbone 1H, 15N and 13C resonance assignments of the 27kDa fluorescent protein mCherry

Authors: Sette, Marco Johnson, Laura Anne Jimenez, Ralph et al.

Contributors: Sette, Marco Johnson, Laura Anne Jimenez, Ralph et al.

Source: Biomol NMR Assign
Sette, M, Johnson, L A, Jimenez, R & Mulder, F A A 2023, 'Backbone 1 H, 15 N and 13 C resonance assignments of the 27kDa fluorescent protein mCherry', Biomolecular NMR Assignments, vol. 17, no. 2, pp. 243-247. https://doi.org/10.1007/s12104-023-10149-z

Subject Terms: Photostability, Red fluorescent protein, Chemical shift, Protein engineering, mCherry, Protein Engineering, Nuclear Magnetic Resonance, Biomolecular, Article, Protein Engineering/methods

File Description: application/pdf

Access URL: https://pubmed.ncbi.nlm.nih.gov/37684490
https://pure.au.dk/ws/files/404550685/s12104-023-10149-z.pdf
https://epub.jku.at/id/9151589
https://doi.org/10.1007/s12104-023-10149-z
https://permalink.obvsg.at/ULI/AC16959346
http://www.scopus.com/inward/record.url?scp=85170046280&partnerID=8YFLogxK
https://pure.au.dk/ws/files/404550685/s12104-023-10149-z.pdf
https://pure.au.dk/portal/en/publications/ccfe6463-2506-4901-819e-1129ddf1a2bd
https://doi.org/10.1007/s12104-023-10149-z

Mining and engineering activity in catalytic amyloids

Authors: Peña-Díaz, Samuel Ferreira, Pedro Ramos, Maria João et al.

Source: Methods in Enzymology ISBN: 9780443236679
Peña-Díaz, S, Ferreira, P, Ramos, M J & Otzen, D E 2024, 'Mining and engineering activity in catalytic amyloids', Methods in Enzymology, vol. 697, pp. 345-422. https://doi.org/10.1016/bs.mie.2024.03.002

Subject Terms: 0301 basic medicine, Pseudomonas/metabolism, Amyloid, Amyloid/chemistry, tau Proteins, Protein Engineering, QM/MM, Catalysis, 03 medical and health sciences, tau Proteins/metabolism, Bacterial Proteins, Bacterial Proteins/chemistry, Pseudomonas, Catalytic Domain, Escherichia coli, Humans, Computational analysis, 0303 health sciences, Escherichia coli Proteins, Escherichia coli Proteins/metabolism, Amyloid formation, alpha-Synuclein/chemistry, 3. Good health, Escherichia coli/genetics, Catalytic assays, alpha-Synuclein, Chromogenic substrates, Functional and pathological amyloid, Protein Engineering/methods

Access URL: https://pubmed.ncbi.nlm.nih.gov/38816129
http://www.scopus.com/inward/record.url?scp=85190791654&partnerID=8YFLogxK
https://doi.org/10.1016/bs.mie.2024.03.002
https://pure.au.dk/portal/en/publications/8843b42e-f8aa-4ac6-bd39-078baef4a0cd

Accelerated Engineering of ELP‐Based Materials through Hybrid Biomimetic‐De Novo Predictive Molecular Design

Authors: Timo Laakko Antti Korkealaakso Burcu Firatligil Yildirir et al.

Contributors: Timo Laakko Antti Korkealaakso Burcu Firatligil Yildirir et al.

Source: Advanced Materials. 36

Subject Terms: computational modeling, Models, Molecular, α-helical conformation, Molecular, protein engineering, Elastin/chemistry, Peptides/chemistry, Protein Engineering, Elastin, machine learning, Models, Biomimetic Materials, Biomimetics, de novo design, Biomimetics/methods, Peptides, Biomimetic Materials/chemistry, Protein Engineering/methods

File Description: application/pdf; fulltext

Access URL: https://pubmed.ncbi.nlm.nih.gov/38710202
https://aaltodoc.aalto.fi/handle/123456789/129633

The road to fully programmable protein catalysis

Authors: Sarah L. Lovelock Rebecca Crawshaw Sophie Basler et al.

Source: Lovelock, S L, Crawshaw, R, Basler, S, Levy, C, Baker, D, Hilvert, D & Green, A P 2022, ' The road to fully programmable protein catalysis ', Nature, vol. 606, no. 7912, pp. 49-58 . https://doi.org/10.1038/s41586-022-04456-z
Nature

Subject Terms: 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, Biotechnology/methods, Biocatalysis, Proteins, Protein Engineering, Protein Engineering/methods, Proteins/chemistry, Biotechnology

Access URL: https://pubmed.ncbi.nlm.nih.gov/35650353
https://research.manchester.ac.uk/en/publications/92554aca-4e60-473d-84f3-c0bd076dd2bf
https://doi.org/10.1038/s41586-022-04456-z

Conjugated Protein Domains as Engineered Scaffold Proteins

Authors: Lenne J. M. Lemmens Christian Ottmann Luc Brunsveld

Source: Bioconjug Chem

Subject Terms: 0301 basic medicine, 0303 health sciences, Proteins, Hydrogels, Hydrogels/chemistry, Protein Engineering, 03 medical and health sciences, Metabolic Engineering, Protein Domains, Animals, Humans, Protein Engineering/methods, Proteins/chemistry, Signal Transduction

Access URL: https://pubs.acs.org/doi/pdf/10.1021/acs.bioconjchem.0c00183
https://pubmed.ncbi.nlm.nih.gov/32374984
https://research.tue.nl/en/publications/25d6b3c7-a88f-4ad1-8e37-ecd1bfdc42a7
https://doi.org/10.1021/acs.bioconjchem.0c00183
https://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.0c00183
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303964
https://research.tue.nl/en/publications/conjugated-protein-domains-as-engineered-scaffold-proteins
https://pubs.acs.org/doi/10.1021/acs.bioconjchem.0c00183
https://www.narcis.nl/publication/RecordID/oai%3Apure.tue.nl%3Apublications%2F25d6b3c7-a88f-4ad1-8e37-ecd1bfdc42a7
https://pubmed.ncbi.nlm.nih.gov/32374984/

Use of the chicken lysozyme 5' matrix attachment region to generate high producer CHO cell lines.

Authors: Girod, P.A. Zahn-Zabal, M. Mermod, N.

Subject Terms: Animals, CHO Cells/metabolism, Chickens, Cricetinae, Cricetulus, Genetic Enhancement/methods, Humans, Immunoglobulin G/biosynthesis, Immunoglobulin G/genetics, Matrix Attachment Region Binding Proteins/genetics, Matrix Attachment Region Binding Proteins/metabolism, Matrix Attachment Regions/genetics, Muramidase/genetics, Muramidase/metabolism, Promoter Regions, Genetic/genetics, Protein Engineering/methods, Recombinant Proteins/biosynthesis, Transfection/methods

Relation: Biotechnology and Bioengineering; 0006-3592[print], 0006-3592[linking]; https://iris.unil.ch/handle/iris/91649; serval:BIB_358EDB26B6C3; 000230178900001

Availability: https://iris.unil.ch/handle/iris/91649
https://doi.org/10.1002/bit.20563

Effect on antibody and T-cell responses of mixing five GMP-produced DNA plasmids and administration with plasmid expressing GM-CSF

Authors: Sedegah, M. Charoenvit, Y. Aguiar, J. et al.

Subject Terms: Animals Antibodies, Protozoan/*biosynthesis Female Granulocyte-Macrophage Colony-Stimulating Factor/*administration & dosage/biosynthesis/genetics Mice Mice, Inbred BALB C Mice, Inbred C57BL Plasmids/*administration & dosage/chemical synthesis/immunology Plasmodium falciparum/*immunology Protein Engineering/methods/*standards Protozoan Vaccines/administration & dosage/genetics/immunology T-Lymphocytes/*drug effects/immunology Vaccines, DNA/*administration & dosage/immunology

Relation: Genes and Immunity; https://iris.unil.ch/handle/iris/60542; serval:BIB_32641F8662E6; 000224732900005

Availability: https://iris.unil.ch/handle/iris/60542
https://doi.org/10.1038/sj.gene.6364125

siRNA-based inhibition specific for mutant SOD1 with single nucleotide alternation in familial ALS, compared with ribozyme and DNA enzyme

Authors: Yokota, T. Miyagishi, M. Hino, T. et al.

Subject Terms: Amyotrophic Lateral Sclerosis/genetics/metabolism/therapy Animals Cell Line DNA, Catalytic/genetics/*metabolism Feasibility Studies Gene Expression Regulation, Enzymologic/genetics Gene Silencing Gene Therapy/methods Humans Kidney/embryology/*metabolism Mice *Mutagenesis, Site-Directed Neuroblastoma/*metabolism Protein Engineering/methods RNA, Catalytic/genetics/*metabolism RNA, Small Interfering/genetics/*metabolism Superoxide Dismutase/*genetics/*metabolism

Relation: Biochemical and Biophysical Research Communications; https://iris.unil.ch/handle/iris/35543; serval:BIB_3A2F08A95BBF; 000188250200041

Availability: https://iris.unil.ch/handle/iris/35543
https://doi.org/10.1016/j.bbrc.2003.12.098

Development of stable cell lines for production or regulated expression using matrix attachment regions.

Authors: Zahn-Zabal, M. Kobr, M. Girod, P.A. et al.

Subject Terms: Animals, CHO Cells, Cell Line, Chickens, Chromatin/genetics, Cricetinae, Extracellular Matrix/metabolism, Gene Expression Regulation, Muramidase/genetics, Muramidase/metabolism, Protein Engineering/methods, Transfection, Transgenes

Relation: Journal of Biotechnology; 0168-1656[print], 0168-1656[linking]; https://iris.unil.ch/handle/iris/174510; serval:BIB_D9E7557AD0B6; 000168113000003

Availability: https://iris.unil.ch/handle/iris/174510
https://doi.org/10.1016/S0168-1656(00)00423-5

A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking

Authors: Hinrichsen, M. Lenz, M. Edwards, J. M. et al.

Contributors: Hinrichsen, M. Lenz, M. Edwards, J. M. et al.

Source: Hinrichsen, M, Lenz, M, Edwards, J M, Miller, O K, Mochrie, S G J, Swain, P S, Schwarz-Linek, U & Regan, L 2017, ' A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking ', Protein engineering, design & selection : PEDS, vol. 30, no. 12, pp. 771-780 . https://doi.org/10.1093/protein/gzx059

Subject Terms: 0301 basic medicine, Post-translational labeling, Fluorescent Dyes/analysis, QH301 Biology, NDAS, Cell Tracking/methods, Saccharomyces cerevisiae, Protein Engineering, Biochemistry, Fluorescence imaging, Saccharomyces cerevisiae/genetics, QH301, 03 medical and health sciences, QD, Fluorescent Dyes, 0303 health sciences, Single-Cell Analysis/methods, Spectrometry, Fluorescence/methods, QD Chemistry, 3. Good health, Spectrometry, Fluorescence, Cell Tracking, Protein engineering, Single-Cell Analysis, Protein Processing, Post-Translational, Protein Engineering/methods

File Description: application/pdf

Access URL: https://academic.oup.com/peds/article-pdf/30/12/771/29098404/gzx059.pdf
https://pubmed.ncbi.nlm.nih.gov/29228311
https://academic.oup.com/peds/article/30/12/771/4710354
https://www.ncbi.nlm.nih.gov/pubmed/29228311
http://europepmc.org/abstract/MED/29228311
https://research-repository.st-andrews.ac.uk/handle/10023/16634
https://pubmed.ncbi.nlm.nih.gov/29228311/
https://research-repository.st-andrews.ac.uk/bitstream/10023/16634/1/Hinrichsen_final.pdf
https://www.pure.ed.ac.uk/ws/files/85692765/A_New_Method_for_Post_translationally_AAM_Hinrichsen_final.pdf
https://hdl.handle.net/20.500.11820/3c278faa-11fa-43d6-a901-eccbfa687e99
https://hdl.handle.net/10023/16634
https://academic.oup.com/peds/article/30/12/771/4710354#supplementary-data

How Does Replacement of the Axial Histidine Ligand in Cytochrome c Peroxidase by Nδ-Methyl Histidine Affect Its Properties and Functions? A Computational Study

Authors: Lee, Calvin W. Z. Bin Mubarak, Muhammad Qadri Effendy Green, Anthony et al.

Source: Lee, C W Z, Bin Mubarak, M Q E, Green, A & De Visser, S 2020, 'How Does Replacement of the Axial Histidine Ligand in Cytochrome c Peroxidase by Nδ-Methyl Histidine Affect Its Properties and Functions? A Computational Study', International Journal of Molecular Sciences, vol. 21, no. 19, 7133, pp. 1-18. https://doi.org/10.3390/ijms21197133

Subject Terms: Density functional theory, Enzyme engineering, Enzyme models, Epoxidation, Heme enzymes, Hydroxylation, Peroxidases, Catalytic Domain, Hydrogen Peroxide/chemistry, Methylhistidines/chemistry, Oxidation-Reduction, Horseradish Peroxidase/chemistry, Computational Biology/methods, Cytochrome-c Peroxidase/chemistry, Hydrogen Bonding, Protein Engineering/methods, Ligands, Heme/chemistry, Catalysis, Iron/chemistry, Ferric Compounds/chemistry

File Description: application/pdf

Availability: https://research.manchester.ac.uk/en/publications/1bff4b52-1e7c-4ce6-af6f-b47b2d7d660b
https://doi.org/10.3390/ijms21197133
https://pure.manchester.ac.uk/ws/files/176857199/245_IntJMolSc2020_07133.pdf

Tailor made proteins against dirt and cancer / Skräddarsydda proteiner mot smuts och cancer

Authors: Alpman, Marie

Source: Forskning och Framsteg. 53(10):27

Towards further reduction and replacement of animal bioassays in prion research by cell and protein misfolding cyclic amplification assays

Authors: Boerner, Susann Wagenführ, Katja Daus, Martin L. et al.

Source: Laboratory Animals. 47:106-115

Subject Terms: PrPSc Proteins/genetics, 0301 basic medicine, PrPSc Proteins/chemistry, Protein Folding, Neuroglia/metabolism, PrPSc Proteins, Blotting, Western, 610 Medizin, Scrapie/metabolism, Cattle Diseases, PrPSc Proteins/metabolism, Protein Engineering, Sensitivity and Specificity, Creutzfeldt-Jakob Syndrome, Electrophoresis Polyacrylamide Gel, Prion Diseases, 03 medical and health sciences, Creutzfeldt-Jakob Syndrome/metabolism, Cricetinae, Animals, Humans, PrPC Proteins, Encephalopathy Bovine Spongiform/metabolism, PrPC Proteins/chemistry, Biological Assay/methods, Disinfection/methods, ddc:610, 0303 health sciences, Sheep, Prion Diseases/metabolism, Cattle Diseases/metabolism, Biological Assay/instrumentation, PrPC Proteins/metabolism, PrPC Proteins/genetics, Neuroglia/pathology, Blotting Western, Disinfection, Encephalopathy, Bovine Spongiform, Prion Diseases/genetics, Sheep/metabolism, Biological Assay, Cattle, Electrophoresis, Polyacrylamide Gel, Protein Engineering/instrumentation, Neuroglia, Protein Engineering/methods, Scrapie

File Description: application/pdf

Access URL: https://edoc.rki.de/bitstream/176904/1840/1/29YuGjA1Aa5A.pdf
https://pubmed.ncbi.nlm.nih.gov/23479773
https://www.ncbi.nlm.nih.gov/pubmed/23479773
https://edoc.rki.de/bitstream/176904/1840/1/29YuGjA1Aa5A.pdf
https://pubmed.ncbi.nlm.nih.gov/23479773/
https://edoc.rki.de/handle/176904/1840
https://lan.sagepub.com/content/47/2/106.long
https://journals.sagepub.com/doi/full/10.1177/0023677213476856

Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coli–derived recombinant human bone morphogenetic protein 2 in rat calvarial defects

Authors: Seong-Ho Choi Chang-Sung Kim Kyunghee Choi et al.

Contributors: Seong-Ho Choi Chang-Sung Kim Kyunghee Choi et al.

Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 112:298-306

Subject Terms: Male, 0301 basic medicine, Skull/surgery, Bone Regeneration, Bone Regeneration/physiology, Bone Morphogenetic Protein 2, Bone Regeneration/drug effects, Protein Engineering, Statistics, Nonparametric, Skull/drug effects, Rats, Sprague-Dawley, 03 medical and health sciences, Hydroxyapatites/chemistry, 0302 clinical medicine, Bone Substitutes/chemistry, Coated Materials, Biocompatible, Osseointegration, Osteogenesis, Biocompatible/chemistry, Osteogenesis/physiology, Escherichia coli, Animals, Humans, Nonparametric, Recombinant Proteins/biosynthesis, Particle Size, Bone Morphogenetic Protein 2/biosynthesis, Bone Morphogenetic Protein 2/administration & dosage, Drug Carriers, Bone Substitutes/administration & dosage, Osseointegration/physiology, Statistics, Skull, Coated Materials, Osseointegration/drug effects, Hydroxyapatites/administration & dosage, Skull/anatomy & histology, Recombinant Proteins, Osteogenesis/drug effects, Rats, 3. Good health, Freeze Drying, Biocompatible/administration & dosage, Bone Substitutes, Sprague-Dawley, Hydroxyapatites, Protein Engineering/methods

File Description: 298~306

Access URL: https://pubmed.ncbi.nlm.nih.gov/21292513
https://www.sciencedirect.com/science/article/pii/S1079210410008577
https://yonsei.pure.elsevier.com/en/publications/bone-formation-of-block-and-particulated-biphasic-calcium-phospha
https://europepmc.org/article/MED/21292513
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94329
https://www.ncbi.nlm.nih.gov/pubmed/21292513

Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants

Authors: Asano, Yasuhisa Dadashipour, Mohammad Yamazaki, Mizue et al.

Source: Protein Engineering, Design and Selection. 24:607-616

Subject Terms: Electrophoresis, 0301 basic medicine, Manihot, Molecular Sequence Data, Protein Engineering, Fluorescence, 03 medical and health sciences, Spectroscopy, Fourier Transform Infrared, Escherichia coli, Directed Molecular Evolution/methods, Amino Acid Sequence, Recombinant Proteins/biosynthesis, Spectroscopy, Plant Proteins/biosynthesis, Aldehyde-Lyases, Plant Proteins, 0303 health sciences, Polyacrylamide Gel, Aldehyde-Lyases/biosynthesis, Spectrometry, Protein Stability, Manihot/enzymology, Circular Dichroism, Temperature, Hydrogen-Ion Concentration, Recombinant Proteins, 3. Good health, Escherichia coli/genetics, Spectrometry, Fluorescence, Solubility, Fourier Transform Infrared, Mutation, Electrophoresis, Polyacrylamide Gel, Directed Molecular Evolution, Sequence Alignment, Protein Engineering/methods

Access URL: https://academic.oup.com/peds/article-pdf/24/8/607/17494398/gzr030.pdf
https://pubmed.ncbi.nlm.nih.gov/21729945
https://www.ncbi.nlm.nih.gov/pubmed/21729945
https://academic.oup.com/peds/article/24/8/607/1508245
http://peds.oxfordjournals.org/content/early/2011/07/04/protein.gzr030.abstract
https://europepmc.org/article/MED/21729945
https://paperity.org/p/41880329/functional-expression-of-a-plant-hydroxynitrile-lyase-in-escherichia-coli-by-directed
https://pubmed.ncbi.nlm.nih.gov/21729945/

Tailoring protein nanomechanics with chemical reactivity

Authors: Beedle, Amy Mora, Marc Lynham, Steven et al.

Contributors: Beedle, Amy Mora, Marc Lynham, Steven et al.

Source: Nat Commun
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Beedle, A E M, Mora, M, Lynham, S, Stirnemann, G & Garcia-Manyes, S 2017, ' Tailoring protein nanomechanics with chemical reactivity ', Nature Communications, vol. 8, no. 1, 15658, pp. 1-11 . https://doi.org/10.1038/ncomms15658

Subject Terms: Models, Molecular, 0301 basic medicine, Protein Folding, Cysteine/chemistry, Oxygen/chemistry, Protein Conformation, Science, Protein Engineering, Article, Mass Spectrometry, 03 medical and health sciences, Models, Escherichia coli, Humans, Cysteine, Disulfides, Sulfhydryl Compounds, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Ultraviolet, 0303 health sciences, Protein Stability, Molecular, Proteins, Hydrogen-Ion Concentration, Oxygen, Spectrophotometry, Escherichia coli/metabolism, Mutation, Thermodynamics, Spectrophotometry, Ultraviolet, Disulfides/chemistry, Protein Engineering/methods, Proteins/chemistry

File Description: application/pdf

Access URL: https://www.nature.com/articles/ncomms15658.pdf
https://pubmed.ncbi.nlm.nih.gov/28585528
https://doaj.org/article/7f39aa321a0a4c9e85b067804b4cb304
https://www.nature.com/articles/ncomms15658
https://www.ncbi.nlm.nih.gov/pubmed/28585528
https://pubmed.ncbi.nlm.nih.gov/28585528/
https://www.nature.com/articles/ncomms15658.pdf
https://core.ac.uk/display/83945145
https://europepmc.org/article/MED/28585528
https://kclpure.kcl.ac.uk/en/publications/1c8ec2f7-12f5-4c20-b12f-7486f3211a3d
https://kclpure.kcl.ac.uk/portal/en/publications/1c8ec2f7-12f5-4c20-b12f-7486f3211a3d
https://hal.science/hal-03826568v1
https://doi.org/10.1038/ncomms15658

Engineering BRET-Sensor Proteins

Authors: Remco Arts Stijn J.A. Aper Maarten Merkx

Source: Enzymes as Sensors

Subject Terms: 0301 basic medicine, Point-of-care diagnostics, Biosensing Techniques, Intracellular imaging, Luminescent Proteins/analysis, Protein Engineering, 7. Clean energy, 03 medical and health sciences, Biosensing Techniques/methods, Fluorescence Resonance Energy Transfer, Animals, Humans, 0303 health sciences, Fluorescence Resonance Energy Transfer/methods, Optical Imaging, Luminescent Measurements/methods, Optical Imaging/methods, Luminescent Proteins, Biosensors, Point-of-Care Testing, Luminescent Measurements, FRET, BRET, Protein engineering, Smartphone, Bioluminescence, Luciferase, Protein Engineering/methods

Access URL: https://pubmed.ncbi.nlm.nih.gov/28336075
https://research.tue.nl/en/publications/e50352a8-49a6-41db-a373-fb3d82ba142a
https://doi.org/10.1016/bs.mie.2017.01.010
https://www.ncbi.nlm.nih.gov/pubmed/28336075
http://www.sciencedirect.com/science/article/pii/S0076687917300368
https://www.narcis.nl/publication/RecordID/oai%3Apure.tue.nl%3Apublications%2Fe50352a8-49a6-41db-a373-fb3d82ba142a
https://research.tue.nl/en/publications/engineering-bret-sensor-proteins
https://www.sciencedirect.com/science/article/pii/S0076687917300368
https://europepmc.org/abstract/MED/28336075

Engineering Antibody Heavy Chain CDR3 to Create a Phage Display Fab Library Rich in Antibodies That Bind Charged Carbohydrates

Authors: Schoonbroodt, Sonia Steukers, Mieke Viswanathan, Malini et al.

Source: The Journal of Immunology. 181:6213-6221

Subject Terms: 0301 basic medicine, Immunology, Molecular Sequence Data, Static Electricity, Bacteriophage M13/immunology, Oligosaccharides, Oligosaccharides/chemistry, Protein Engineering, Sciences de la santé humaine, Immunologie & maladie infectieuse, Lewis a oligosaccharide, Oligosaccharides/immunology, Immunoglobulin Fab Fragments, Mice, 03 medical and health sciences, Lewis Blood Group Antigens, Peptide Library, Bacteriophage M13/chemistry, Immunology and Allergy, Animals, Humans, Immunoglobulin Heavy Chains/genetics, Immunology & infectious disease, Amino Acid Sequence, Complementarity Determining Regions/chemistry, Human health sciences, Complementarity Determining Regions/genetics, Bacteriophage M13/genetics, 0303 health sciences, Oligosaccharides/genetics, Complementarity Determining Regions, 3. Good health, Carbohydrate Sequence, Drug Design, Immunoglobulin Fab Fragments/chemistry, Binding Sites, Antibody, Immunoglobulin Fab Fragments/genetics, Immunoglobulin Heavy Chains, Immunoglobulin Heavy Chains/chemistry, Protein Engineering/methods, Antibody Diversity, Bacteriophage M13

Access URL: http://www.jimmunol.org/content/181/9/6213.full.pdf
https://pubmed.ncbi.nlm.nih.gov/18941211
http://www.jimmunol.org/content/181/9/6213.full
http://www.ncbi.nlm.nih.gov/pubmed/18941211
http://www.jimmunol.org/content/181/9/6213

Challenges Associated with Heterologous Expression of Flavobacterium psychrophilum Proteins in Escherichia coli

Authors: Shah, Devendra H Cain, Kenneth D Wiens, Gregory D et al.

Source: Marine Biotechnology. 10:719-730

Subject Terms: 0301 basic medicine, Escherichia coli - metabolism, Inclusion Bodies - metabolism, Flavobacterium - genetics, Protein Engineering, Flavobacterium, Flavobacterium - metabolism, Hemolysin Proteins, 03 medical and health sciences, Bacterial Proteins, Hemolysin Proteins - genetics, Models, Recombinant Proteins - metabolism, Escherichia coli, Protein Engineering - methods, Cloning, Molecular, Codon, Inclusion Bodies, 0303 health sciences, Models, Statistical, Vibrio parahaemolyticus - genetics, Bacterial, Molecular, Gene Expression Regulation, Bacterial, Statistical, Recombinant Proteins, 3. Good health, Recombinant Proteins - genetics, Codon - metabolism, Bacterial Proteins - metabolism, Gene Expression Regulation, Solubility, Hemolysin Proteins - metabolism, Vibrio parahaemolyticus - metabolism, Vibrio parahaemolyticus, Bacterial Proteins - genetics, Escherichia coli - genetics, Cloning

Access URL: https://pubmed.ncbi.nlm.nih.gov/18551344
https://link.springer.com/article/10.1007/s10126-008-9111-z
https://link.springer.com/content/pdf/10.1007%2Fs10126-008-9111-z.pdf