Suchergebnisse - "POLYMERASE chain reaction"

Development and validation of oligonucleotide sets for real time RT-PCR detection and next generation sequencing, of re-emerged sylvatic Dengue virus 2 strains.

Autoren: Dia NA Ndiaye M Balde D et al.

Quelle: Journal of virological methods [J Virol Methods] 2025 Dec; Vol. 338, pp. 115218. Date of Electronic Publication: 2025 Jul 05.

Publikationsart: Journal Article; Validation Study

Info zur Zeitschrift: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

MeSH-Schlagworte: Dengue Virus*/genetics , Dengue Virus*/isolation & purification , Dengue Virus*/classification , High-Throughput Nucleotide Sequencing*/methods , Dengue*/virology , Dengue*/diagnosis , Real-Time Polymerase Chain Reaction*/methods , Oligonucleotides*/genetics , Reverse Transcriptase Polymerase Chain Reaction*/methods, Humans ; RNA, Viral/genetics ; Sensitivity and Specificity ; DNA Primers/genetics ; Multiplex Polymerase Chain Reaction ; Genome, Viral ; Senegal ; Serogroup

Development of rapid and simple FCoV RNA detection systems using RT-PCR and RT-RPA combined with STH-PAS to diagnose FIP in cats.

Autoren: Doki T Ohno M Kaku M et al.

Quelle: Journal of virological methods [J Virol Methods] 2025 Dec; Vol. 338, pp. 115214. Date of Electronic Publication: 2025 Jun 28.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

MeSH-Schlagworte: Coronavirus, Feline*/genetics , Coronavirus, Feline*/isolation & purification , Feline Infectious Peritonitis*/diagnosis , Feline Infectious Peritonitis*/virology , RNA, Viral*/genetics , RNA, Viral*/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction*/methods , Reverse Transcriptase Polymerase Chain Reaction*/veterinary , Nucleic Acid Amplification Techniques*/methods , Nucleic Acid Amplification Techniques*/veterinary , Molecular Diagnostic Techniques*/methods, Animals ; Cats ; Sensitivity and Specificity ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/veterinary

Validation of a dual-purpose conventional PCR strategy for SARS-CoV-2 detection and lineage screening in resource-limited laboratories.

Autoren: Iñiguez-Gutiérrez L Magaña-Torres MT Ruiz-Carrillo JD et al.

Quelle: Molecular biology reports [Mol Biol Rep] 2025 Oct 11; Vol. 52 (1), pp. 1011. Date of Electronic Publication: 2025 Oct 11.

Publikationsart: Journal Article; Validation Study

Info zur Zeitschrift: Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE

MeSH-Schlagworte: SARS-CoV-2*/genetics , SARS-CoV-2*/isolation & purification , SARS-CoV-2*/classification , COVID-19*/diagnosis , COVID-19*/virology , COVID-19 Nucleic Acid Testing*/methods , Polymerase Chain Reaction*/methods, Humans ; Spike Glycoprotein, Coronavirus/genetics ; Nasopharynx/virology ; RNA, Viral/genetics ; Multiplex Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/methods

Novel real-time quantitative RT-PCR for detection of PRRSV-1 and PRRSV-2 strains circulating in China: A study based on whole-genome characteristics and epidemiological features.

Autoren: Gong B Xiang L Li J et al.

Quelle: Veterinary journal (London, England : 1997) [Vet J] 2025 Dec; Vol. 314, pp. 106482. Date of Electronic Publication: 2025 Nov 04.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Balliere Tindall Country of Publication: England NLM ID: 9706281 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2971 (Electronic) Linking ISSN: 10900233 NLM ISO Abbreviation: Vet J Subsets: MEDLINE

MeSH-Schlagworte: Porcine respiratory and reproductive syndrome virus*/genetics , Porcine respiratory and reproductive syndrome virus*/isolation & purification , Porcine respiratory and reproductive syndrome virus*/classification , Porcine Reproductive and Respiratory Syndrome*/epidemiology , Porcine Reproductive and Respiratory Syndrome*/virology , Porcine Reproductive and Respiratory Syndrome*/diagnosis , Real-Time Polymerase Chain Reaction*/veterinary , Real-Time Polymerase Chain Reaction*/methods , Genome, Viral*, Animals ; China/epidemiology ; Swine ; Sensitivity and Specificity ; Reverse Transcriptase Polymerase Chain Reaction/veterinary

Development of a duplex chamber digital PCR to quantify twelve genetically modified maize events.

Autoren: Kim EH Cho YS Kim B et al.

Quelle: GM crops & food [GM Crops Food] 2025 Dec; Vol. 16 (1), pp. 527-538. Date of Electronic Publication: 2025 Aug 21.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101572655 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2164-5701 (Electronic) Linking ISSN: 21645698 NLM ISO Abbreviation: GM Crops Food Subsets: MEDLINE

MeSH-Schlagworte: Zea mays*/genetics , Plants, Genetically Modified*/genetics , Polymerase Chain Reaction*/methods, Real-Time Polymerase Chain Reaction/methods ; Reproducibility of Results

Development of droplet digital PCR (ddPCR) for the detection and quantification of Mycoplasma gallisepticum in duck flocks.

Autoren: Zhou L Wang A Song F et al.

Quelle: Microbial pathogenesis [Microb Pathog] 2025 Dec; Vol. 209, pp. 108123. Date of Electronic Publication: 2025 Oct 20.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE

MeSH-Schlagworte: Mycoplasma gallisepticum*/isolation & purification , Mycoplasma gallisepticum*/genetics , Mycoplasma Infections*/veterinary , Mycoplasma Infections*/diagnosis , Mycoplasma Infections*/microbiology , Ducks*/microbiology , Poultry Diseases*/diagnosis , Poultry Diseases*/microbiology , Polymerase Chain Reaction*/methods, Animals ; Sensitivity and Specificity ; Reproducibility of Results ; DNA, Bacterial/genetics ; DNA Primers/genetics ; Real-Time Polymerase Chain Reaction/methods ; Limit of Detection

Validation of H5 influenza virus subtyping RT-qPCR assay and low prevalence of H5 detection in 2024-2025 influenza virus season.

Autoren: Bacsik DJ Mills MG Monroe LD et al.

Quelle: Journal of clinical microbiology [J Clin Microbiol] 2025 Nov 12; Vol. 63 (11), pp. e0041525. Date of Electronic Publication: 2025 Oct 21.

Publikationsart: Journal Article; Validation Study

Info zur Zeitschrift: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE

MeSH-Schlagworte: Influenza, Human*/virology , Influenza, Human*/epidemiology , Influenza, Human*/diagnosis , Real-Time Polymerase Chain Reaction*/methods , Influenza A Virus, H5N1 Subtype*/genetics , Influenza A Virus, H5N1 Subtype*/isolation & purification , Influenza A Virus, H5N1 Subtype*/classification , Influenza A virus*/genetics , Influenza A virus*/isolation & purification , Influenza A virus*/classification, Humans ; Retrospective Studies ; Prevalence ; Animals ; Nasopharynx/virology ; Sensitivity and Specificity ; Seasons ; Multiplex Polymerase Chain Reaction/methods ; United States/epidemiology

Rapid diagnosis of viral infections and application to infection control.

Autoren: Endo M Tokuyama-Toda R Shirota T et al.

Quelle: BMC infectious diseases [BMC Infect Dis] 2025 Nov 03; Vol. 25 (1), pp. 1474. Date of Electronic Publication: 2025 Nov 03.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: Virus Diseases*/diagnosis , Virus Diseases*/virology , Real-Time Polymerase Chain Reaction*/methods , Infection Control*/methods , Viruses*/genetics , Viruses*/isolation & purification , Viruses*/classification , Molecular Diagnostic Techniques*/methods, Humans ; Multiplex Polymerase Chain Reaction/methods ; Sensitivity and Specificity

Hierarchical integration of mNGS, PCR, and other conventional methods for precision TB diagnostics.

Autoren: Zhao Y Du L Song J et al.

Quelle: Microbiology spectrum [Microbiol Spectr] 2025 Oct 07; Vol. 13 (10), pp. e0193125. Date of Electronic Publication: 2025 Sep 11.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE

MeSH-Schlagworte: Mycobacterium tuberculosis*/genetics , Mycobacterium tuberculosis*/isolation & purification , Tuberculosis*/diagnosis , Tuberculosis*/microbiology , High-Throughput Nucleotide Sequencing*/methods , Polymerase Chain Reaction*/methods , Molecular Diagnostic Techniques*/methods, Humans ; Retrospective Studies ; Sensitivity and Specificity ; Female ; Male ; Middle Aged ; Adult ; Real-Time Polymerase Chain Reaction/methods ; ROC Curve ; Aged ; Metagenomics/methods

Letter to the Editor: Enhancing early detection of HPV associated oropharyngeal cancer: a comparative study of NGS, ddPCR, and qPCR.

Autoren: Abilaji S Narenkumar J

Quelle: Oral oncology [Oral Oncol] 2025 Oct; Vol. 169, pp. 107461. Date of Electronic Publication: 2025 Jun 27.

Publikationsart: Letter; Comparative Study; Editorial

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: England NLM ID: 9709118 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0593 (Electronic) Linking ISSN: 13688375 NLM ISO Abbreviation: Oral Oncol Subsets: MEDLINE

MeSH-Schlagworte: Oropharyngeal Neoplasms*/virology , Oropharyngeal Neoplasms*/diagnosis , Real-Time Polymerase Chain Reaction*/methods , High-Throughput Nucleotide Sequencing*/methods , Early Detection of Cancer*/methods , Papillomavirus Infections*/diagnosis , Papillomavirus Infections*/virology , Papillomavirus Infections*/complications , Papillomaviridae*/genetics , Papillomaviridae*/isolation & purification, Humans ; Polymerase Chain Reaction/methods ; Sensitivity and Specificity

Optimization of a multiplex RT-PCR assay for the detection of human parechovirus, enterovirus, adenovirus type 14, and respiratory syncytial virus using the panther fusion system.

Autoren: Roloff EA Patel KM Raj P

Quelle: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2025 Sep; Vol. 113 (1), pp. 116768. Date of Electronic Publication: 2025 Feb 22.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: Multiplex Polymerase Chain Reaction*/methods , Parechovirus*/isolation & purification , Parechovirus*/genetics , Adenoviruses, Human*/isolation & purification , Adenoviruses, Human*/genetics , Enterovirus*/isolation & purification , Enterovirus*/genetics , Reverse Transcriptase Polymerase Chain Reaction*/methods, Humans ; Sensitivity and Specificity ; Picornaviridae Infections/diagnosis ; Picornaviridae Infections/virology ; Real-Time Polymerase Chain Reaction/methods ; Respiratory Syncytial Virus, Human/isolation & purification ; Respiratory Syncytial Virus, Human/genetics

Comparison of PCR-Based Methods for the Detection of Canned Tuna Species.

Autoren: Castanon CP Hernandez D Khetrapal AN et al.

Quelle: Journal of food science [J Food Sci] 2025 Sep; Vol. 90 (9), pp. e70424.

Publikationsart: Journal Article; Comparative Study

Info zur Zeitschrift: Publisher: Wiley on behalf of the Institute of Food Technologists Country of Publication: United States NLM ID: 0014052 Publication Model: Print Cited Medium: Internet ISSN: 1750-3841 (Electronic) Linking ISSN: 00221147 NLM ISO Abbreviation: J Food Sci Subsets: MEDLINE

MeSH-Schlagworte: Tuna*/genetics , Tuna*/classification , Real-Time Polymerase Chain Reaction*/methods , Food, Preserved*/analysis , DNA Barcoding, Taxonomic*/methods , Seafood*/analysis , Polymerase Chain Reaction*/methods, Animals ; Multiplex Polymerase Chain Reaction/methods ; Species Specificity ; Food Contamination/analysis ; DNA ; Food Labeling

[Quantification of Influenza A/B Virus and RSV Validation Standards using Digital PCR].

Transliterierter Titel: İnfluenza A/B Virüs ve RSV Validasyon Standartlarının Dijital PCR ile Kantitasyonu.

Autoren: Bulgurcu A Sayiner AA

Quelle: Mikrobiyoloji bulteni [Mikrobiyol Bul] 2025 Jul; Vol. 59 (3), pp. 320-337.

Publikationsart: Journal Article; Validation Study; English Abstract

Info zur Zeitschrift: Publisher: Ankara Mikrobiyoloji Dernegi Country of Publication: Turkey NLM ID: 7503830 Publication Model: Print Cited Medium: Print ISSN: 0374-9096 (Print) Linking ISSN: 03749096 NLM ISO Abbreviation: Mikrobiyol Bul Subsets: MEDLINE

MeSH-Schlagworte: Influenza A virus*/isolation & purification , Influenza A virus*/genetics , Influenza B virus*/isolation & purification , Influenza B virus*/genetics , RNA, Viral*/analysis , RNA, Viral*/isolation & purification , Respiratory Syncytial Virus Infections*/diagnosis , Respiratory Syncytial Virus Infections*/virology , Influenza, Human*/diagnosis , Influenza, Human*/virology , Polymerase Chain Reaction*/methods , Polymerase Chain Reaction*/standards , Respiratory Syncytial Virus, Human*/isolation & purification , Respiratory Syncytial Virus, Human*/genetics , Respiratory Syncytial Viruses*/isolation & purification , Respiratory Syncytial Viruses*/genetics, Humans ; Reproducibility of Results ; Nasopharynx/virology ; Sensitivity and Specificity ; Reference Standards ; Real-Time Polymerase Chain Reaction/standards ; Real-Time Polymerase Chain Reaction/methods

Germplex PCR and massively parallel sequencing: A dynamic duo for detecting bacteria of biodefense and public health importance.

Autoren: Dias VHG Gomes PDSFC Damaso CR et al.

Quelle: Forensic science international. Genetics [Forensic Sci Int Genet] 2026 Feb; Vol. 81, pp. 103372. Date of Electronic Publication: 2025 Oct 09.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101317016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0326 (Electronic) Linking ISSN: 18724973 NLM ISO Abbreviation: Forensic Sci Int Genet Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing* , DNA, Bacterial*/genetics , Multiplex Polymerase Chain Reaction* , Bacteria*/genetics , Bacteria*/isolation & purification, Humans ; DNA Primers ; Public Health ; Bioterrorism ; RNA, Ribosomal, 16S/genetics ; Biological Warfare Agents ; Polymerase Chain Reaction

Quantitative Real-Time PCR for Trypanosoma cruzi Infection Diagnosis and Treatment Response Monitoring of Patients with Chagas Disease.

Autoren: Ramirez JC Moreira OC

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2982, pp. 163-176.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Trypanosoma cruzi*/genetics , Trypanosoma cruzi*/isolation & purification , Chagas Disease*/diagnosis , Chagas Disease*/parasitology , Chagas Disease*/drug therapy , Chagas Disease*/blood , Real-Time Polymerase Chain Reaction*/methods, Humans ; DNA, Protozoan/genetics ; DNA, Protozoan/blood ; Parasite Load/methods ; Multiplex Polymerase Chain Reaction/methods

Candidiasis: a novel molecular differential diagnosis through real-time PCR followed by high-resolution thermal denaturation.

Autoren: Schatz CY Taverna CG Refojo N et al.

Quelle: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2026 Jan; Vol. 114 (1), pp. 117058. Date of Electronic Publication: 2025 Aug 11.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: Real-Time Polymerase Chain Reaction*/methods , Candidiasis*/diagnosis , Candidiasis*/microbiology , Molecular Diagnostic Techniques*/methods , Candida*/genetics , Candida*/isolation & purification , Candida*/classification, Humans ; Sensitivity and Specificity ; Candida albicans/genetics ; Candida albicans/isolation & purification ; Candida parapsilosis/genetics ; Candida parapsilosis/isolation & purification ; Diagnosis, Differential ; Candida auris/genetics ; Candida auris/isolation & purification ; Transition Temperature ; DNA Primers/genetics ; Multiplex Polymerase Chain Reaction/methods ; Nucleic Acid Denaturation

Duplex droplet digital PCR enables simultaneous quantification of algal giant virus DSLLAV1 and virophage DSLV8 in natural and laboratory samples.

Autoren: Chu T Yu J Wang Q et al.

Quelle: Journal of virological methods [J Virol Methods] 2026 Jan; Vol. 339, pp. 115273. Date of Electronic Publication: 2025 Oct 03.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

MeSH-Schlagworte: Virophages*/genetics , Virophages*/isolation & purification , Polymerase Chain Reaction*/methods , Giant Viruses*/isolation & purification , Giant Viruses*/genetics, Sensitivity and Specificity ; Lakes/virology ; DNA Primers/genetics ; Real-Time Polymerase Chain Reaction/methods ; Genome, Viral

A dual-target real-time PCR for proactive detection of Mpox variants.

Autoren: Lee TD O'Dwyer A Chan M et al.

Quelle: Journal of virological methods [J Virol Methods] 2026 Jan; Vol. 339, pp. 115260. Date of Electronic Publication: 2025 Sep 05.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

MeSH-Schlagworte: Real-Time Polymerase Chain Reaction*/methods , Monkeypox virus*/genetics , Monkeypox virus*/isolation & purification , Molecular Diagnostic Techniques*/methods, Humans ; Mutation ; Sensitivity and Specificity ; California ; Multiplex Polymerase Chain Reaction/methods

Quantification of Tissue and Circulating MicroRNAs by Droplet Digital PCR.

Autoren: Broseghini E Fontana B Durante G et al.

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2969, pp. 195-233.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Circulating MicroRNA*/genetics , Circulating MicroRNA*/analysis , MicroRNAs*/genetics , MicroRNAs*/blood , Polymerase Chain Reaction*/methods, Humans ; Paraffin Embedding ; Real-Time Polymerase Chain Reaction/methods ; Extracellular Vesicles/genetics

Development and application of a dual RT-qPCR assay for differentiating of goose astrovirus genotypes 1 and 2.

Autoren: Liu W Wang J Liu L et al.

Quelle: Research in veterinary science [Res Vet Sci] 2025 Sep; Vol. 193, pp. 105753. Date of Electronic Publication: 2025 Jun 07.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE

MeSH-Schlagworte: Geese*/virology , Astroviridae Infections*/veterinary , Astroviridae Infections*/virology , Astroviridae Infections*/diagnosis , Poultry Diseases*/virology , Poultry Diseases*/diagnosis , Real-Time Polymerase Chain Reaction*/veterinary , Real-Time Polymerase Chain Reaction*/methods, Animals ; Genotype ; Sensitivity and Specificity ; Avastrovirus/genetics ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction/veterinary