Suchergebnisse - "NUCLEOTIDE sequencing"

Evaluation of false positive and false negative errors in targeted next generation sequencing.

Autoren: Moon Y Kim YH Kim JK et al.

Quelle: Genome biology [Genome Biol] 2025 Dec 01; Vol. 26 (1), pp. 409. Date of Electronic Publication: 2025 Dec 01.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: BioMed Central Ltd Country of Publication: England NLM ID: 100960660 Publication Model: Electronic Cited Medium: Internet ISSN: 1474-760X (Electronic) Linking ISSN: 14747596 NLM ISO Abbreviation: Genome Biol Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/standards , Sequence Analysis, DNA*/methods , Sequence Analysis, DNA*/standards, Humans ; False Positive Reactions ; False Negative Reactions ; Female

Molecular Definition of the Novel HLA-DQB1*04:107 Allele Identified in Brazil Through Next-Generation Sequencing.

Autoren: Perroni KTR Massi FP Hirata BKB et al.

Quelle: HLA [HLA] 2025 Dec; Vol. 106 (6), pp. e70496.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Wiley Blackwell Country of Publication: England NLM ID: 101675570 Publication Model: Print Cited Medium: Internet ISSN: 2059-2310 (Electronic) Linking ISSN: 20592302 NLM ISO Abbreviation: HLA Subsets: MEDLINE

MeSH-Schlagworte: Alleles* , Exons* , High-Throughput Nucleotide Sequencing* , HLA-DQ beta-Chains*/genetics , Polymorphism, Single Nucleotide*, Humans ; Base Sequence ; Brazil ; Histocompatibility Testing ; Sequence Analysis, DNA

Next-Generation Sequencing in Infectious-Disease Diagnostics: Economic, Regulatory, and Clinical Pathways to Adoption.

Autoren: Osei Sekyere J; Department of Medical Microbiology, School of Medicine, University of Pretoria, Pretoria, South Africa.; Institute of Biomarker Research and Department of Clinical Development, Medical Diagnostic Laboratories LLC, Hamilton Township, New Jersey, USA.

Quelle: MicrobiologyOpen [Microbiologyopen] 2025 Dec; Vol. 14 (6), pp. e70104.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101588314 Publication Model: Print Cited Medium: Internet ISSN: 2045-8827 (Electronic) Linking ISSN: 20458827 NLM ISO Abbreviation: Microbiologyopen Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/economics , High-Throughput Nucleotide Sequencing*/methods , Communicable Diseases*/diagnosis , Molecular Diagnostic Techniques*/economics , Molecular Diagnostic Techniques*/methods, Humans ; Whole Genome Sequencing/economics ; Whole Genome Sequencing/methods ; Polymerase Chain Reaction/economics ; Polymerase Chain Reaction/methods

Developing Consensus for a More Provider-Friendly Next-Generation Sequencing Molecular Biomarker Report: A Joint Consensus Recommendation of the Association for Molecular Pathology and College of American Pathologists.

Autoren: Gibson J El Achi H Altenburger D et al.

Quelle: The Journal of molecular diagnostics : JMD [J Mol Diagn] 2025 Dec; Vol. 27 (12), pp. 1123-1136. Date of Electronic Publication: 2025 Sep 26.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 100893612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1943-7811 (Electronic) Linking ISSN: 15251578 NLM ISO Abbreviation: J Mol Diagn Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/standards , Biomarkers, Tumor*/genetics , Pathology, Molecular*/methods , Pathology, Molecular*/standards, Humans ; Consensus ; Pathologists ; United States

Illumina MiSeq and iSeq platforms yield comparable results for viral genomic sequencing.

Autoren: Wagner DD Nabakooza G Momin N et al.

Quelle: Journal of virological methods [J Virol Methods] 2025 Dec; Vol. 338, pp. 115202. Date of Electronic Publication: 2025 Jun 13.

Publikationsart: Journal Article; Comparative Study

Info zur Zeitschrift: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/instrumentation , SARS-CoV-2*/genetics , Genome, Viral* , Poliovirus*/genetics , Norovirus*/genetics, Humans ; Polymorphism, Single Nucleotide ; COVID-19/virology ; COVID-19/diagnosis

Next-generation sequencing in breast cancer: current clinical applications and future directions.

Autoren: Mohd Zuhdi NF Siddig A Mohd Nafi SN et al.

Quelle: Annals of medicine [Ann Med] 2025 Dec; Vol. 57 (1), pp. 2569989. Date of Electronic Publication: 2025 Oct 06.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Informa Healthcare Country of Publication: England NLM ID: 8906388 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2060 (Electronic) Linking ISSN: 07853890 NLM ISO Abbreviation: Ann Med Subsets: MEDLINE

MeSH-Schlagworte: Breast Neoplasms*/genetics , Breast Neoplasms*/diagnosis , Breast Neoplasms*/drug therapy , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/trends, Humans ; Female ; Precision Medicine/methods ; Precision Medicine/trends ; Biomarkers, Tumor/genetics ; Circulating Tumor DNA/genetics ; Circulating Tumor DNA/blood ; Genomics/methods ; Receptor, ErbB-2/genetics ; Prognosis ; Single-Cell Analysis/methods

Brave New World: Analysis of Early Clinical Experience With Molecular Sequencing in the Treatment of Rectal Cancer.

Autoren: Yee JT Eltahir AA Oduyale OK et al.

Quelle: Diseases of the colon and rectum [Dis Colon Rectum] 2025 Dec 01; Vol. 68 (12), pp. 1414-1421. Date of Electronic Publication: 2025 Aug 26.

Publikationsart: Journal Article; Video-Audio Media

Info zur Zeitschrift: Publisher: Lippincott Country of Publication: United States NLM ID: 0372764 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-0358 (Electronic) Linking ISSN: 00123706 NLM ISO Abbreviation: Dis Colon Rectum Subsets: MEDLINE

MeSH-Schlagworte: Rectal Neoplasms*/genetics , Rectal Neoplasms*/therapy , Rectal Neoplasms*/pathology , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/statistics & numerical data , Circulating Tumor DNA*/genetics , Circulating Tumor DNA*/blood , Genetic Testing*/methods , Genetic Testing*/statistics & numerical data, Humans ; Female ; Male ; Retrospective Studies ; Middle Aged ; Aged ; Neoadjuvant Therapy/methods ; Neoplasm Recurrence, Local/genetics ; Mutation ; Biomarkers, Tumor/genetics ; Adult

Rapid NGS Analysis on Google Cloud Platform: Performance Benchmark and User Tutorial.

Autoren: Franzoso E Santorsola M Lescai F

Quelle: Clinical and translational science [Clin Transl Sci] 2025 Nov; Vol. 18 (11), pp. e70416.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: WileyBlackwell Pub Country of Publication: United States NLM ID: 101474067 Publication Model: Print Cited Medium: Internet ISSN: 1752-8062 (Electronic) Linking ISSN: 17528054 NLM ISO Abbreviation: Clin Transl Sci Subsets: MEDLINE

MeSH-Schlagworte: Cloud Computing* , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/economics, Humans ; Benchmarking ; Computational Biology/methods ; Software

The CODEX Approach: High-Throughput Sequencing of the Cox-1 Barcode Fragment in Neogastropods (Mollusca, Gastropoda).

Autoren: Puillandre N Zuccon D Abdelkrim J et al.

Quelle: Molecular ecology resources [Mol Ecol Resour] 2025 Nov; Vol. 25 (8), pp. e70031. Date of Electronic Publication: 2025 Aug 11.

Publikationsart: Journal Article; Evaluation Study

Info zur Zeitschrift: Publisher: Blackwell Country of Publication: England NLM ID: 101465604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-0998 (Electronic) Linking ISSN: 1755098X NLM ISO Abbreviation: Mol Ecol Resour Subsets: MEDLINE

MeSH-Schlagworte: DNA Barcoding, Taxonomic*/methods , DNA Barcoding, Taxonomic*/economics , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/economics , Gastropoda*/genetics , Gastropoda*/classification , Electron Transport Complex IV*/genetics , Cyclooxygenase 1*/genetics, Animals ; Sequence Analysis, DNA/methods ; Molecular Sequence Data

Cost-effectiveness analysis of metagenomic next-generation sequencing versus traditional bacterial cultures for postoperative central nervous system infections in critical care settings: a prospective pilot study.

Autoren: Tian Y Xu N Chen Y et al.

Quelle: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2025 Oct 28; Vol. 15, pp. 1710412. Date of Electronic Publication: 2025 Oct 28 (Print Publication: 2025).

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE

MeSH-Schlagworte: Cost-Benefit Analysis* , High-Throughput Nucleotide Sequencing*/economics , High-Throughput Nucleotide Sequencing*/methods , Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/microbiology , Central Nervous System Infections*/economics , Metagenomics*/economics , Metagenomics*/methods , Critical Care*/economics , Bacteria*/genetics , Bacteria*/isolation & purification , Bacteria*/classification , Postoperative Complications*/microbiology , Postoperative Complications*/diagnosis , Bacteriological Techniques*/economics , Bacteriological Techniques*/methods, Humans ; Pilot Projects ; Prospective Studies ; Female ; Male ; Middle Aged ; Aged ; Adult ; Intensive Care Units ; China ; Cost-Effectiveness Analysis

Implementation of an ISO 15189 accredited next generation sequencing service for cell-free total nucleic acid (cfTNA) analysis to facilitate driver mutation reporting in blood: the experience of a clinical diagnostic laboratory.

Autoren: Werner R Crosbie R Dorney M et al.

Quelle: Journal of clinical pathology [J Clin Pathol] 2025 Oct 17; Vol. 78 (11), pp. 783-791. Date of Electronic Publication: 2025 Oct 17.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: BMJ Pub. Group Country of Publication: England NLM ID: 0376601 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-4146 (Electronic) Linking ISSN: 00219746 NLM ISO Abbreviation: J Clin Pathol Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/standards , High-Throughput Nucleotide Sequencing*/methods , Neoplasms*/genetics , Neoplasms*/blood , Neoplasms*/diagnosis , Mutation* , Cell-Free Nucleic Acids*/blood , Cell-Free Nucleic Acids*/genetics , Biomarkers, Tumor*/genetics , Biomarkers, Tumor*/blood , Circulating Tumor DNA*/blood , Circulating Tumor DNA*/genetics, Humans ; Liquid Biopsy ; Reproducibility of Results ; DNA Mutational Analysis/standards ; Laboratories, Clinical/standards

Deciphering the impact of contaminating microbiota in DNA extraction reagents on metagenomic next-generation sequencing workflows.

Autoren: Lai Z-L Su Y-D Lin H-H et al.

Quelle: Microbiology spectrum [Microbiol Spectr] 2025 Oct 07; Vol. 13 (10), pp. e0311924. Date of Electronic Publication: 2025 Aug 20.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE

MeSH-Schlagworte: Metagenomics*/methods , Metagenomics*/standards , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/standards , DNA Contamination* , Microbiota*/genetics , DNA, Bacterial*/genetics , DNA, Bacterial*/isolation & purification, Humans ; Workflow ; Indicators and Reagents ; Bacteria/genetics ; Bacteria/classification ; Bacteria/isolation & purification

HPVPool-Seq: a genotype-guided pooling strategy for cost-effective next-generation sequencing detection of HPV integration in cervical samples.

Autoren: Guan W Liu S Chen Y et al.

Quelle: Microbiology spectrum [Microbiol Spectr] 2025 Oct 07; Vol. 13 (10), pp. e0139925. Date of Electronic Publication: 2025 Aug 19.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/economics , Papillomavirus Infections*/virology , Papillomavirus Infections*/diagnosis , Papillomaviridae*/genetics , Papillomaviridae*/isolation & purification , Papillomaviridae*/classification , Cervix Uteri*/virology , Virus Integration*/genetics, Humans ; Female ; Genotype ; Cost-Benefit Analysis ; DNA, Viral/genetics ; Uterine Cervical Neoplasms/virology ; Uterine Cervical Neoplasms/diagnosis

Real-time raw signal genomic analysis using fully integrated memristor hardware.

Autoren: He P Wang S Mao R et al.

Quelle: Nature computational science [Nat Comput Sci] 2025 Oct; Vol. 5 (10), pp. 940-951. Date of Electronic Publication: 2025 Sep 12.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Springer Nature Country of Publication: United States NLM ID: 101775476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2662-8457 (Electronic) Linking ISSN: 26628457 NLM ISO Abbreviation: Nat Comput Sci Subsets: MEDLINE

MeSH-Schlagworte: Genomics*/methods , Genomics*/instrumentation , High-Throughput Nucleotide Sequencing*/instrumentation , High-Throughput Nucleotide Sequencing*/methods , Sequence Analysis, DNA*/instrumentation , Sequence Analysis, DNA*/methods, Software ; Humans ; Algorithms ; Metagenomics

Bridging the Diagnostic Gap in Hereditary Cancers with Simple, Cost-Effective, High-Throughput RNA Splicing Analysis.

Autoren: Amiot J Levacher C Thibaut LM et al.

Quelle: The Journal of molecular diagnostics : JMD [J Mol Diagn] 2025 Oct; Vol. 27 (10), pp. 954-968. Date of Electronic Publication: 2025 Jul 23.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 100893612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1943-7811 (Electronic) Linking ISSN: 15251578 NLM ISO Abbreviation: J Mol Diagn Subsets: MEDLINE

MeSH-Schlagworte: RNA Splicing*/genetics , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/economics , Neoplasms*/genetics , Neoplasms*/diagnosis, Humans ; Cost-Benefit Analysis ; Female ; Genetic Predisposition to Disease ; Sensitivity and Specificity ; Exons

High-throughput nucleotide sequencing reveals new circulatory miRNA genes in cervical cancer patients.

Autoren: Abbas HH Rodrigues FAE Krishnan D et al.

Quelle: Computers in biology and medicine [Comput Biol Med] 2025 Oct; Vol. 197 (Pt B), pp. 111116. Date of Electronic Publication: 2025 Sep 22.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 1250250 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0534 (Electronic) Linking ISSN: 00104825 NLM ISO Abbreviation: Comput Biol Med Subsets: MEDLINE

MeSH-Schlagworte: Uterine Cervical Neoplasms*/genetics , Uterine Cervical Neoplasms*/blood , High-Throughput Nucleotide Sequencing* , Gene Expression Regulation, Neoplastic* , Biomarkers, Tumor*/blood , Biomarkers, Tumor*/genetics , MicroRNAs*/blood , MicroRNAs*/genetics , Circulating MicroRNA*/genetics , Circulating MicroRNA*/blood , RNA, Neoplasm*/blood , RNA, Neoplasm*/genetics, Humans ; Female ; Middle Aged ; Adult ; Gene Expression Profiling

Accurate and Cost-Efficient Whole Genome Sequencing of Hepatitis B Virus Using Nanopore.

Autoren: Stenbäck JB Schmidt D Noborg U et al.

Quelle: Journal of medical virology [J Med Virol] 2025 Oct; Vol. 97 (10), pp. e70630.

Publikationsart: Journal Article; Evaluation Study

Info zur Zeitschrift: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE

MeSH-Schlagworte: Hepatitis B virus*/genetics , Hepatitis B virus*/isolation & purification , Whole Genome Sequencing*/methods , Whole Genome Sequencing*/economics , Genome, Viral* , Nanopores* , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/economics , Nanopore Sequencing*/methods , Nanopore Sequencing*/economics, Humans ; DNA, Viral/genetics ; Hepatitis B/virology ; Genotype

Epidermal Growth Factor Receptor Aberrations Identified by Next-Generation Sequencing in Patients with Metastatic Cancers.

Autoren: Shin M Choi DH Jung J et al.

Quelle: Cancer research and treatment [Cancer Res Treat] 2025 Oct; Vol. 57 (4), pp. 932-941. Date of Electronic Publication: 2025 Feb 21.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Korean Cancer Association Country of Publication: Korea (South) NLM ID: 101155137 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2005-9256 (Electronic) Linking ISSN: 15982998 NLM ISO Abbreviation: Cancer Res Treat Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , Mutation* , Neoplasms*/genetics , Neoplasms*/pathology , Biomarkers, Tumor*/genetics, Humans ; ErbB Receptors/genetics ; Female ; Male ; Middle Aged ; Neoplasm Metastasis ; Aged ; Adult ; Microsatellite Instability ; Aged, 80 and over ; Gene Amplification

Tracing the evolution of sequencing into the era of genomic medicine.

Autoren: Mardis ER Wilson RK

Quelle: Nature reviews. Genetics [Nat Rev Genet] 2025 Oct; Vol. 26 (10), pp. 719-734. Date of Electronic Publication: 2025 Aug 15.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 100962779 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1471-0064 (Electronic) Linking ISSN: 14710056 NLM ISO Abbreviation: Nat Rev Genet Subsets: MEDLINE

MeSH-Schlagworte: Genomics*/methods , Genomics*/trends , Genome, Human* , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/trends , Sequence Analysis, DNA*/methods , Sequence Analysis, DNA*/trends, Humans ; Precision Medicine

An Innovative Binding-Protein-Based dsRNA Extraction Method: Comparison of Cost-Effectiveness of Virus Detection Methods Using High-Throughput Sequencing.

Autoren: Fall ML Xu D Lemoyne P et al.

Quelle: Molecular ecology resources [Mol Ecol Resour] 2025 Oct; Vol. 25 (7), pp. e14111. Date of Electronic Publication: 2025 May 15.

Publikationsart: Journal Article; Comparative Study

Info zur Zeitschrift: Publisher: Blackwell Country of Publication: England NLM ID: 101465604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-0998 (Electronic) Linking ISSN: 1755098X NLM ISO Abbreviation: Mol Ecol Resour Subsets: MEDLINE

MeSH-Schlagworte: RNA, Double-Stranded*/isolation & purification , RNA, Double-Stranded*/genetics , High-Throughput Nucleotide Sequencing*/methods , High-Throughput Nucleotide Sequencing*/economics , RNA, Viral*/isolation & purification , RNA, Viral*/genetics, Cost-Benefit Analysis