Search Results - "Isoamylase"

Noncatalytic functions of ISOAMYLASE 1 and 2 affect the proportion of insoluble and soluble α-polyglucans in maize.

Authors: Hennen-Bierwagen TA James MG Newton CJ et al.

Source: The Plant cell [Plant Cell] 2025 Oct 08; Vol. 37 (10).

Publication Type: Journal Article

Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 9208688 Publication Model: Print Cited Medium: Internet ISSN: 1532-298X (Electronic) Linking ISSN: 10404651 NLM ISO Abbreviation: Plant Cell Subsets: MEDLINE

MeSH Terms: Zea mays*/enzymology , Zea mays*/genetics , Zea mays*/metabolism , Glucans*/metabolism , Isoamylase*/metabolism , Isoamylase*/genetics , Plant Proteins*/metabolism , Plant Proteins*/genetics, Solubility ; Starch/metabolism ; Endosperm/metabolism

Superior thermostability and divalent cation sensitivity of isoamylase CMI294C from Cyanidioschyzon merolae.

Authors: Okada K Someya T Osanai T

Source: Plant molecular biology [Plant Mol Biol] 2025 Jul 31; Vol. 115 (4), pp. 99. Date of Electronic Publication: 2025 Jul 31.

Publication Type: Journal Article

Journal Info: Publisher: Kluwer Academic Country of Publication: Netherlands NLM ID: 9106343 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-5028 (Electronic) Linking ISSN: 01674412 NLM ISO Abbreviation: Plant Mol Biol Subsets: MEDLINE

MeSH Terms: Rhodophyta*/enzymology , Rhodophyta*/genetics , Isoamylase*/metabolism , Isoamylase*/genetics , Isoamylase*/chemistry , Cations, Divalent*/pharmacology , Cations, Divalent*/metabolism, Amylopectin/metabolism ; Enzyme Stability ; Temperature

The transcription factor StERF75 negatively regulates starch biosynthesis by targeting Isoamylase in potato.

Authors: Ai J Yang M Zou J et al.

Source: International journal of biological macromolecules [Int J Biol Macromol] 2025 Sep; Vol. 321 (Pt 1), pp. 146118. Date of Electronic Publication: 2025 Jul 20.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE

MeSH Terms: Solanum tuberosum*/genetics , Solanum tuberosum*/metabolism , Starch*/biosynthesis , Transcription Factors*/metabolism , Transcription Factors*/genetics , Transcription Factors*/chemistry , Plant Proteins*/genetics , Plant Proteins*/metabolism , Plant Proteins*/chemistry , Isoamylase*/genetics , Isoamylase*/metabolism, Gene Expression Regulation, Plant ; Promoter Regions, Genetic

Amylopectin branch trimming and biosynthesis elucidated by the rice isoamylase ISA1-ISA2 heterocomplex.

Authors: Fan R Guan Z Zhou G et al.

Source: Nature communications [Nat Commun] 2025 Jul 01; Vol. 16 (1), pp. 5638. Date of Electronic Publication: 2025 Jul 01.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

MeSH Terms: Oryza*/enzymology , Oryza*/metabolism , Oryza*/genetics , Amylopectin*/biosynthesis , Amylopectin*/metabolism , Plant Proteins*/metabolism , Plant Proteins*/chemistry , Plant Proteins*/genetics , Isoamylase*/metabolism , Isoamylase*/chemistry , Isoamylase*/genetics, Protein Multimerization ; Cryoelectron Microscopy ; Models, Molecular

Optimization of Isoamylase Production in Escherichia coli Under Different Nutrient Limitation Conditions.

Authors: Wang D Bai Y Chen M et al.

Source: Biotechnology journal [Biotechnol J] 2025 Apr; Vol. 20 (4), pp. e70009.

Publication Type: Journal Article

Journal Info: Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 101265833 Publication Model: Print Cited Medium: Internet ISSN: 1860-7314 (Electronic) Linking ISSN: 18606768 NLM ISO Abbreviation: Biotechnol J Subsets: MEDLINE

MeSH Terms: Escherichia coli*/genetics , Escherichia coli*/metabolism , Escherichia coli*/growth & development , Escherichia coli*/enzymology , Isoamylase*/genetics , Isoamylase*/metabolism , Isoamylase*/biosynthesis , Nutrients*/metabolism, Nitrogen/metabolism ; Fermentation ; Phosphorus/metabolism ; Carbon/metabolism ; Biomass ; Culture Media/chemistry ; Batch Cell Culture Techniques ; Magnesium/metabolism ; Sulfur/metabolism

Effect of Debranching Enzymes on Self-Assembly Kinetics and Physicochemical Characteristics of Short-Chain Glucan Aggregates (SCGAs).

Authors: Kim MY Oh SM Shin JS et al.

Source: Journal of agricultural and food chemistry [J Agric Food Chem] 2025 Apr 16; Vol. 73 (15), pp. 9157-9164. Date of Electronic Publication: 2025 Apr 02.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE

MeSH Terms: Glucans*/chemistry , Glycoside Hydrolases*/chemistry , Isoamylase*/chemistry, Kinetics ; Starch/chemistry ; Crystallization

Analysis of starch structure, functional properties, and the underlying molecular mechanism in SeaRice86 (SR86).

Authors: Huang Y Yang X Cui Y et al.

Source: Carbohydrate polymers [Carbohydr Polym] 2025 Oct 15; Vol. 366, pp. 123881. Date of Electronic Publication: 2025 Jun 09.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE

MeSH Terms: Starch*/chemistry , Starch*/metabolism , Oryza*/chemistry , Oryza*/genetics , Oryza*/metabolism, Amylose/chemistry ; Viscosity ; Starch Synthase/metabolism ; Starch Synthase/genetics ; Glycoside Hydrolases/genetics ; Glycoside Hydrolases/metabolism ; Amylopectin/chemistry ; Gene Expression Regulation, Plant ; 1,4-alpha-Glucan Branching Enzyme/metabolism ; 1,4-alpha-Glucan Branching Enzyme/genetics ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Isoamylase/genetics ; Isoamylase/metabolism

Mutations in starch BRANCHING ENZYME 2a suppress the traits caused by the loss of ISOAMYLASE1 in barley.

Authors: Matsushima R Hisano H Kim JS et al.

Source: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Aug 31; Vol. 137 (9), pp. 212. Date of Electronic Publication: 2024 Aug 31.

Publication Type: Journal Article

Journal Info: Publisher: Springer Country of Publication: Germany NLM ID: 0145600 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-2242 (Electronic) Linking ISSN: 00405752 NLM ISO Abbreviation: Theor Appl Genet Subsets: MEDLINE

MeSH Terms: Hordeum*/genetics , Hordeum*/enzymology , Hordeum*/growth & development , Phenotype* , Starch*/metabolism , Endosperm*/genetics , 1,4-alpha-Glucan Branching Enzyme*/genetics , 1,4-alpha-Glucan Branching Enzyme*/metabolism , Mutation* , Isoamylase*/genetics , Isoamylase*/metabolism, Plant Proteins/genetics ; Plant Proteins/metabolism

Interactions of separately conserved α-(1→6) glucosidases that participate in maize endosperm starch biosynthesis.

Authors: Boehlein SK Hennen-Bierwagen TA Shuler SL et al.

Source: Plant physiology [Plant Physiol] 2025 Sep 30; Vol. 199 (2).

Publication Type: Journal Article

Journal Info: Publisher: American Society of Plant Biologists Country of Publication: United States NLM ID: 0401224 Publication Model: Print Cited Medium: Internet ISSN: 1532-2548 (Electronic) Linking ISSN: 00320889 NLM ISO Abbreviation: Plant Physiol Subsets: MEDLINE

MeSH Terms: Zea mays*/enzymology , Zea mays*/genetics , Zea mays*/metabolism , Endosperm*/metabolism , Endosperm*/enzymology , Endosperm*/genetics , Starch*/biosynthesis , Plant Proteins*/metabolism , Plant Proteins*/genetics , Glycoside Hydrolases*/metabolism , Glycoside Hydrolases*/genetics, Isoamylase/metabolism ; Isoamylase/genetics ; Mutation/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Gene Expression Regulation, Plant

Synthesis of isomaltooligosaccharides using 4-O-α-d-isomaltooligosaccharylmaltooligosaccharide 1,4-α-isomaltooligosaccharohydrolase.

Authors: Kitagawa N Watanabe H Mori T et al.

Source: Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2023 Nov 21; Vol. 87 (12), pp. 1495-1504.

Publication Type: Journal Article

Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 9205717 Publication Model: Print Cited Medium: Internet ISSN: 1347-6947 (Electronic) Linking ISSN: 09168451 NLM ISO Abbreviation: Biosci Biotechnol Biochem Subsets: MEDLINE

MeSH Terms: Isomaltose* , Isoamylase*, Oligosaccharides ; Glucans ; Starch

Branched oligosaccharides cause atypical starch granule initiation in Arabidopsis chloroplasts.

Authors: Heutinck AJM Camenisch S Fischer-Stettler M et al.

Source: Plant physiology [Plant Physiol] 2025 Feb 07; Vol. 197 (2).

Publication Type: Journal Article

Journal Info: Publisher: American Society of Plant Biologists Country of Publication: United States NLM ID: 0401224 Publication Model: Print Cited Medium: Internet ISSN: 1532-2548 (Electronic) Linking ISSN: 00320889 NLM ISO Abbreviation: Plant Physiol Subsets: MEDLINE

MeSH Terms: Arabidopsis*/metabolism , Arabidopsis*/genetics , Starch*/metabolism , Oligosaccharides*/metabolism , Chloroplasts*/metabolism , Chloroplasts*/ultrastructure, Arabidopsis Proteins/metabolism ; Arabidopsis Proteins/genetics ; Mutation ; Starch Synthase/metabolism ; Starch Synthase/genetics ; Isoamylase/metabolism ; Isoamylase/genetics

Distribution of Aromatic Amino Acid Residues in Substrate-Binding Regions Modulates Substrate Specificity of Microbial Debranching Enzymes.

Authors: Tian Y Kong H Ban X et al.

Source: Journal of agricultural and food chemistry [J Agric Food Chem] 2023 Aug 02; Vol. 71 (30), pp. 11544-11554. Date of Electronic Publication: 2023 Jul 18.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE

MeSH Terms: Isoamylase*/metabolism , Glycogen Debranching Enzyme System*/chemistry, Amylopectin/metabolism ; Substrate Specificity ; Starch/chemistry ; Glycogen/metabolism

Producing 2-O-α-D-glucopyranosyl-L-ascorbic acid by modified cyclodextrin glucosyltransferase and isoamylase.

Authors: Tao X Su L Chen S et al.

Source: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Feb; Vol. 107 (4), pp. 1233-1241. Date of Electronic Publication: 2023 Jan 23.

Publication Type: Journal Article

Journal Info: Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE

MeSH Terms: Isoamylase* , Paenibacillus*/genetics, Mutagenesis, Site-Directed ; Glucosyltransferases/genetics ; Glucosyltransferases/metabolism ; Kinetics ; Substrate Specificity ; Ascorbic Acid

Foliar Application of dsRNA Targeting Endogenous Potato ( Solanum tuberosum ) Isoamylase Genes ISA1, ISA2, and ISA3 Confers Transgenic Phenotype.

Authors: Simon I Persky Z Avital A et al.

Source: International journal of molecular sciences [Int J Mol Sci] 2022 Dec 22; Vol. 24 (1). Date of Electronic Publication: 2022 Dec 22.

Publication Type: Journal Article

Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

MeSH Terms: Isoamylase*/genetics , Isoamylase*/metabolism , Solanum tuberosum*/genetics , Solanum tuberosum*/metabolism, RNA, Double-Stranded/genetics ; Starch/metabolism ; Phenotype ; Sucrose ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism

Mutation in BEIIb mitigates the negative effect of the mutation in ISA1 on grain filling and amyloplast formation in rice.

Authors: Nagamatsu S Wada T Matsushima R et al.

Source: Plant molecular biology [Plant Mol Biol] 2022 Mar; Vol. 108 (4-5), pp. 497-512. Date of Electronic Publication: 2022 Jan 27.

Publication Type: Journal Article

Journal Info: Publisher: Kluwer Academic Country of Publication: Netherlands NLM ID: 9106343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-5028 (Electronic) Linking ISSN: 01674412 NLM ISO Abbreviation: Plant Mol Biol Subsets: MEDLINE

MeSH Terms: 1,4-alpha-Glucan Branching Enzyme/*genetics , Isoamylase/*genetics , Oryza/*genetics , Plastids/*physiology, 1,4-alpha-Glucan Branching Enzyme/metabolism ; Edible Grain ; Genotype ; Isoamylase/metabolism ; Mutation ; Oryza/enzymology ; Oryza/metabolism

[Optimization of maltooligosyl fructofuranosidesproduction by recombinant β-cyclodextrin glycosyltransferase].

Authors: Huang Y Yang Y Xia W et al.

Source: Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2021 Apr 25; Vol. 37 (4), pp. 1415-1424.

Publication Type: Journal Article

Journal Info: Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9426463 Publication Model: Print Cited Medium: Internet ISSN: 1872-2075 (Electronic) Linking ISSN: 10003061 NLM ISO Abbreviation: Sheng Wu Gong Cheng Xue Bao Subsets: MEDLINE

MeSH Terms: Glucosyltransferases* , Isoamylase*, Hydrogen-Ion Concentration ; Starch

Bio characterization of purified isoamylase from Rhizopus oryzae .

Authors: Ghosh B Lahiri D Nag M et al.

Source: Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2020; Vol. 50 (5), pp. 453-459. Date of Electronic Publication: 2019 Dec 23.

Publication Type: Journal Article

Journal Info: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9607037 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2297 (Electronic) Linking ISSN: 10826068 NLM ISO Abbreviation: Prep Biochem Biotechnol Subsets: MEDLINE

MeSH Terms: Fungal Proteins/*chemistry , Isoamylase/*chemistry , Rhizopus oryzae/*enzymology, Enzyme Assays ; Fungal Proteins/isolation & purification ; Fungal Proteins/metabolism ; Glycogen/chemistry ; Glycogen/metabolism ; Hydrogen-Ion Concentration ; Isoamylase/isolation & purification ; Isoamylase/metabolism ; Kinetics ; Molecular Docking Simulation ; Protein Binding ; Substrate Specificity ; Temperature

Heterologous co-expression in E. coli of isoamylase genes from cassava Manihot esculenta Crantz 'KU50' achieves enzyme-active heteromeric complex formation.

Authors: Panpetch P Field RA Limpaseni T

Source: Plant molecular biology [Plant Mol Biol] 2018 Mar; Vol. 96 (4-5), pp. 417-427. Date of Electronic Publication: 2018 Jan 29.

Publication Type: Journal Article

Journal Info: Publisher: Kluwer Academic Country of Publication: Netherlands NLM ID: 9106343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-5028 (Electronic) Linking ISSN: 01674412 NLM ISO Abbreviation: Plant Mol Biol Subsets: MEDLINE

MeSH Terms: Genes, Plant* , Protein Multimerization*, Escherichia coli/*metabolism , Isoamylase/*genetics , Manihot/*enzymology , Manihot/*genetics, Amino Acid Sequence ; Cloning, Molecular ; Isoamylase/chemistry ; Isoamylase/metabolism ; Molecular Weight ; Plant Proteins/chemistry ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Recombinant Proteins/metabolism ; Recombination, Genetic/genetics ; Substrate Specificity

Cloning of the full-length isoamylase3 gene from cassava Manihot esculenta Crantz 'KU50' and its heterologous expression in E. coli.

Authors: Panpetch P Field RA Limpaseni T

Source: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2018 Nov; Vol. 132, pp. 281-286. Date of Electronic Publication: 2018 Sep 13.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE

MeSH Terms: Gene Expression* , Genes, Plant*, Escherichia coli/*genetics , Isoamylase/*genetics , Manihot/*enzymology , Manihot/*genetics, Amino Acid Motifs ; Amino Acid Sequence ; Cloning, Molecular ; Conserved Sequence ; Isoamylase/chemistry ; Models, Molecular ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism

Simultaneous silencing of isoamylases ISA1, ISA2 and ISA3 by multi-target RNAi in potato tubers leads to decreased starch content and an early sprouting phenotype.

Authors: Ferreira SJ Senning M Fischer-Stettler M et al.

Source: PloS one [PLoS One] 2017 Jul 14; Vol. 12 (7), pp. e0181444. Date of Electronic Publication: 2017 Jul 14 (Print Publication: 2017).

Publication Type: Journal Article

Journal Info: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

MeSH Terms: RNA Interference*, Isoamylase/*metabolism , Plant Proteins/*metabolism , Starch/*metabolism, Hexoses/metabolism ; Isoamylase/antagonists & inhibitors ; Isoamylase/genetics ; Phenotype ; Plant Leaves/metabolism ; Plant Proteins/antagonists & inhibitors ; Plant Proteins/genetics ; Plant Tubers/metabolism ; Plants, Genetically Modified/metabolism ; Protein Isoforms/antagonists & inhibitors ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; RNA, Small Interfering/metabolism ; Seedlings/physiology ; Solanum tuberosum/growth & development ; Solanum tuberosum/metabolism ; Sucrose/metabolism