Výsledky vyhľadávania - "Immunoenzyme Techniques"

Development and validation of a VP7-specific EIA for determining the potency and stability of inactivated rotavirus vaccine.

Autori: Moon SS Wang H Brown K a ďalší

Zdroj: Journal of virological methods [J Virol Methods] 2025 Feb; Vol. 332, pp. 115079. Date of Electronic Publication: 2024 Nov 26.

Spôsob vydávania: Journal Article; Validation Study

Informácie o časopise: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

Výrazy zo slovníka MeSH: Rotavirus Vaccines*/immunology , Rotavirus Vaccines*/administration & dosage , Antigens, Viral*/immunology , Vaccines, Inactivated*/immunology , Vaccine Potency* , Capsid Proteins*/immunology , Capsid Proteins*/genetics , Immunoenzyme Techniques*/methods, Antibodies, Monoclonal/immunology ; Antibodies, Viral/immunology ; Antibodies, Viral/blood ; Drug Stability ; Limit of Detection ; Rotavirus/immunology ; Sensitivity and Specificity ; Humans

Toward the validation of an alternative method for endocrine monitoring in sharks: insights from testosterone analyses in the skin of bycatch individuals.

Autori: Carbajal A Hua-Monclús J Serres-Corral P a ďalší

Zdroj: Integrative zoology [Integr Zool] 2025 Nov; Vol. 20 (6), pp. 1333-1339. Date of Electronic Publication: 2024 Jul 17.

Spôsob vydávania: Journal Article; Validation Study; Letter

Informácie o časopise: Publisher: Wiley Publishing Asia Pty Ltd Country of Publication: Australia NLM ID: 101492420 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1749-4877 (Electronic) Linking ISSN: 17494869 NLM ISO Abbreviation: Integr Zool Subsets: MEDLINE

Výrazy zo slovníka MeSH: Sharks*/physiology , Sharks*/metabolism , Testosterone*/metabolism , Testosterone*/analysis , Skin*/chemistry, Animals ; Male ; Immunoenzyme Techniques/veterinary ; Immunoenzyme Techniques/methods

False-positive reactivity of fourth-generation HIV enzyme immunoassay caused by COVID-19 and other infections.

Autori: Ramadan HK Salah AE Mohamed ZR a ďalší

Zdroj: HIV medicine [HIV Med] 2025 Oct; Vol. 26 (10), pp. 1485-1492. Date of Electronic Publication: 2025 Jul 01.

Spôsob vydávania: Journal Article; Review

Informácie o časopise: Publisher: Wiley Country of Publication: England NLM ID: 100897392 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1468-1293 (Electronic) Linking ISSN: 14642662 NLM ISO Abbreviation: HIV Med Subsets: MEDLINE

Výrazy zo slovníka MeSH: HIV Infections*/diagnosis , HIV Infections*/immunology , COVID-19*/diagnosis , COVID-19*/immunology, Humans ; False Positive Reactions ; Immunoenzyme Techniques/methods ; Immunoenzyme Techniques/standards ; SARS-CoV-2/immunology

Evaluation of different standard and modified two-tier testing strategies for the laboratory diagnosis of lyme borreliosis in a European setting.

Autori: Hoeve-Bakker BJA Kerkhof K Heron M a ďalší

Zdroj: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2024 Dec; Vol. 43 (12), pp. 2397-2406. Date of Electronic Publication: 2024 Oct 07.

Spôsob vydávania: Journal Article; Evaluation Study

Informácie o časopise: Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE

Výrazy zo slovníka MeSH: Lyme Disease*/diagnosis , Sensitivity and Specificity* , Antibodies, Bacterial*/blood , Immunoenzyme Techniques*/methods , Immunoenzyme Techniques*/standards, Humans ; Retrospective Studies ; Immunoglobulin M/blood ; Male ; Female ; Europe ; Serologic Tests/methods ; Immunoblotting/methods ; Middle Aged ; Adult ; Aged ; Borrelia burgdorferi/immunology ; Immunoglobulin G/blood ; Clinical Laboratory Techniques/methods ; Clinical Laboratory Techniques/standards ; Adolescent ; Young Adult

Tumor Microvessels with Specific Morphology as a Prognostic Factor in Esophageal Squamous Cell Carcinoma.

Autori: Tun HT Fujisawa M Ohara T a ďalší

Zdroj: Annals of surgical oncology [Ann Surg Oncol] 2025 Oct; Vol. 32 (10), pp. 8024-8036. Date of Electronic Publication: 2025 Jul 10.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Springer Country of Publication: United States NLM ID: 9420840 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1534-4681 (Electronic) Linking ISSN: 10689265 NLM ISO Abbreviation: Ann Surg Oncol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Esophageal Neoplasms*/pathology , Esophageal Neoplasms*/blood supply , Esophageal Neoplasms*/metabolism , Esophageal Neoplasms*/mortality , Carcinoma, Squamous Cell*/blood supply , Carcinoma, Squamous Cell*/pathology , Carcinoma, Squamous Cell*/metabolism , Carcinoma, Squamous Cell*/secondary , Carcinoma, Squamous Cell*/mortality , Neovascularization, Pathologic*/pathology , Neovascularization, Pathologic*/metabolism , Microvessels*/pathology, Humans ; Male ; Female ; Prognosis ; Middle Aged ; Aged ; Survival Rate ; Neoplasm Staging ; Microvascular Density ; Vascular Endothelial Growth Factor A/metabolism ; Biomarkers, Tumor/metabolism ; Follow-Up Studies ; Lymphatic Metastasis ; Immunoenzyme Techniques ; Neoplasm Invasiveness ; Adult ; Esophageal Squamous Cell Carcinoma ; Platelet Endothelial Cell Adhesion Molecule-1/metabolism

Development of a convenient homogeneous enzyme immunoassay for urinary creatinine measurement.

Autori: Xiong X Wang Y Lu L a ďalší

Zdroj: Talanta [Talanta] 2025 Oct 01; Vol. 293, pp. 128046. Date of Electronic Publication: 2025 Mar 29.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE

Výrazy zo slovníka MeSH: Creatinine*/urine , Urinalysis*/methods, Humans ; Immunoenzyme Techniques/methods ; Limit of Detection ; Glucosephosphate Dehydrogenase/chemistry ; Glucosephosphate Dehydrogenase/metabolism

Evaluation of a commercial Histoplasma antigen detection enzyme immunoassay for the follow-up of histoplasmosis treatment in people living with HIV from Argentina.

Autori: Messina FA Marin E Caceres DH a ďalší

Zdroj: Medical mycology [Med Mycol] 2024 Dec 27; Vol. 63 (1).

Spôsob vydávania: Journal Article; Evaluation Study

Informácie o časopise: Publisher: Oxford University Press Country of Publication: England NLM ID: 9815835 Publication Model: Print Cited Medium: Internet ISSN: 1460-2709 (Electronic) Linking ISSN: 13693786 NLM ISO Abbreviation: Med Mycol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Histoplasmosis*/drug therapy , Histoplasmosis*/diagnosis , Histoplasmosis*/urine , Antigens, Fungal*/urine , Antigens, Fungal*/blood , Histoplasma*/immunology , Immunoenzyme Techniques*/methods , Itraconazole*/therapeutic use , HIV Infections*/complications , HIV Infections*/drug therapy , Antifungal Agents*/therapeutic use, Humans ; Male ; Adult ; Female ; Argentina ; Middle Aged ; Amphotericin B/therapeutic use ; Galactose/analogs & derivatives ; Follow-Up Studies ; Mannans/blood ; Mannans/urine ; Treatment Outcome

Integration of Immunosyringe Sensors with Bar-Chart Chips for Prick-and-Read Testing of Prostate Specific Antigen.

Autori: Li C Liu J Almanza A a ďalší

Zdroj: Analytical chemistry [Anal Chem] 2024 Dec 24; Vol. 96 (51), pp. 20267-20276. Date of Electronic Publication: 2024 Dec 09.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Prostate-Specific Antigen*/blood , Lab-On-A-Chip Devices* , Biosensing Techniques* , Immunoenzyme Techniques*/instrumentation, Syringes ; Point-of-Care Systems ; Platinum/chemistry ; Disposable Equipment

Development and Application of a Fully Automated Chemiluminescence Enzyme Immunoassay for the Detection of Antibodies Against Porcine Circovirus 3 Cap.

Autori: Wang L Li D Zeng D a ďalší

Zdroj: Viruses [Viruses] 2024 Dec 17; Vol. 16 (12). Date of Electronic Publication: 2024 Dec 17.

Spôsob vydávania: Journal Article; Research Support, Non-U.S. Gov't

Informácie o časopise: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE

Výrazy zo slovníka MeSH: Circovirus*/immunology , Antibodies, Viral*/blood , Antibodies, Viral*/immunology , Circoviridae Infections*/diagnosis , Circoviridae Infections*/veterinary , Sensitivity and Specificity* , Swine Diseases*/diagnosis , Swine Diseases*/virology , Swine Diseases*/immunology , Immunoenzyme Techniques*/methods, Animals ; Swine ; Luminescent Measurements/methods ; Capsid Proteins/immunology

Clinical Utility of Helicobacter pylori Stool Antigen Testing with a Bioluminescent Enzyme Immunoassay Using a Fecal Occult Blood Test Container.

Autori: Imagawa A Takahashi S Mabe K

Zdroj: Internal medicine (Tokyo, Japan) [Intern Med] 2024 Dec 15; Vol. 63 (24), pp. 3271-3275. Date of Electronic Publication: 2024 May 09.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Japanese Society of Internal Medicine Country of Publication: Japan NLM ID: 9204241 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1349-7235 (Electronic) Linking ISSN: 09182918 NLM ISO Abbreviation: Intern Med Subsets: MEDLINE

Výrazy zo slovníka MeSH: Helicobacter pylori*/isolation & purification , Helicobacter pylori*/immunology , Antigens, Bacterial*/analysis , Helicobacter Infections*/diagnosis , Feces*/microbiology , Feces*/chemistry , Immunoenzyme Techniques*/methods , Occult Blood* , Luminescent Measurements*/methods, Humans ; Female ; Male ; Middle Aged ; Aged ; Adult ; Aged, 80 and over ; Endoscopy, Digestive System/methods

Evaluation of a Chemiluminescent Enzyme Immunoassay for the Detection of Prostaglandin E-Major Urinary Metabolite (PGE-MUM).

Autori: Takagi J Moriyama K Arihiro S a ďalší

Zdroj: Journal of clinical laboratory analysis [J Clin Lab Anal] 2024 Oct; Vol. 38 (19-20), pp. e25102. Date of Electronic Publication: 2024 Sep 16.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Wiley Country of Publication: United States NLM ID: 8801384 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-2825 (Electronic) Linking ISSN: 08878013 NLM ISO Abbreviation: J Clin Lab Anal Subsets: MEDLINE

Výrazy zo slovníka MeSH: Luminescent Measurements*/methods , Immunoenzyme Techniques*/methods , Limit of Detection*, Humans ; Reproducibility of Results ; Female ; Male ; Adult ; Middle Aged ; Prostaglandins E/urine ; Prostaglandins E/metabolism ; Radioimmunoassay/methods ; Young Adult ; Adolescent ; Aged

Impact of the transition from radioimmunoassay (RIA) to chemiluminescent enzyme immunoassay (CLEIA) for the measurement of plasma aldosterone concentration (PAC) on the diagnosis of primary aldosteronism (PA) via retrospective analyses in Okinawa, Japan.

Autori: Honma KI Nakayama Y Tamaki A a ďalší

Zdroj: Endocrine journal [Endocr J] 2024 Sep 02; Vol. 71 (9), pp. 895-906. Date of Electronic Publication: 2024 Jun 21.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Japan Endocrine Society Country of Publication: Japan NLM ID: 9313485 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1348-4540 (Electronic) Linking ISSN: 09188959 NLM ISO Abbreviation: Endocr J Subsets: MEDLINE

Výrazy zo slovníka MeSH: Hyperaldosteronism*/diagnosis , Hyperaldosteronism*/blood , Aldosterone*/blood , Radioimmunoassay*/methods , Radioimmunoassay*/standards , Immunoenzyme Techniques*/methods , Immunoenzyme Techniques*/standards, Humans ; Japan ; Female ; Middle Aged ; Retrospective Studies ; Male ; Aged ; Adult ; Luminescent Measurements/methods

Optimization and application of an immuoperoxidase monolayer assay for the detection of PRRSV.

Autori: Li P Guan C Wang L a ďalší

Zdroj: BMC veterinary research [BMC Vet Res] 2025 Jul 16; Vol. 21 (1), pp. 470. Date of Electronic Publication: 2025 Jul 16.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE

Výrazy zo slovníka MeSH: Porcine respiratory and reproductive syndrome virus*/isolation & purification , Porcine respiratory and reproductive syndrome virus*/immunology , Porcine Reproductive and Respiratory Syndrome*/diagnosis , Porcine Reproductive and Respiratory Syndrome*/virology, Animals ; Swine ; Antibodies, Monoclonal/immunology ; Sensitivity and Specificity ; Immunoenzyme Techniques/veterinary ; Immunoenzyme Techniques/methods ; Antibodies, Viral ; Reproducibility of Results

Comparative Analysis of the Clarus Aspergillus Galactomannan Enzyme Immunoassay Prototype for the Diagnosis of Invasive Pulmonary Aspergillosis in Bronchoalveolar Lavage Fluid.

Autori: Sedik S Boyer J Egger M a ďalší

Zdroj: Mycopathologia [Mycopathologia] 2024 Jul 18; Vol. 189 (4), pp. 67. Date of Electronic Publication: 2024 Jul 18.

Spôsob vydávania: Journal Article; Comparative Study

Informácie o časopise: Publisher: Kluwer Academic Country of Publication: Netherlands NLM ID: 7505689 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-0832 (Electronic) Linking ISSN: 0301486X NLM ISO Abbreviation: Mycopathologia Subsets: MEDLINE

Výrazy zo slovníka MeSH: Mannans*/analysis , Galactose*/analogs & derivatives , Bronchoalveolar Lavage Fluid*/microbiology , Bronchoalveolar Lavage Fluid*/chemistry , Invasive Pulmonary Aspergillosis*/diagnosis , Immunoenzyme Techniques*/methods , Aspergillus*/isolation & purification , Sensitivity and Specificity*, Humans ; Prospective Studies ; Cross-Sectional Studies ; Middle Aged ; Male ; Female ; Adult ; Aged ; ROC Curve ; Young Adult

Diagnosis of Coccidioidomycosis with the Second-Generation Miravista IgG and IgM Enzyme Immunoassay and the Role of Adding Miravista Coccidioides Antigen Detection to Immunodiagnostic Assays.

Autori: Kassis C Eric H Nicolas B a ďalší

Zdroj: Medical mycology [Med Mycol] 2024 Jul 04; Vol. 62 (7).

Spôsob vydávania: Journal Article; Comparative Study

Informácie o časopise: Publisher: Oxford University Press Country of Publication: England NLM ID: 9815835 Publication Model: Print Cited Medium: Internet ISSN: 1460-2709 (Electronic) Linking ISSN: 13693786 NLM ISO Abbreviation: Med Mycol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Coccidioidomycosis*/diagnosis , Coccidioidomycosis*/immunology , Antigens, Fungal*/immunology , Antigens, Fungal*/blood , Immunoglobulin M*/blood , Immunoglobulin G*/blood , Coccidioides*/immunology , Sensitivity and Specificity* , Immunoenzyme Techniques*/methods , Antibodies, Fungal*/blood, Humans ; Retrospective Studies ; Male ; Middle Aged ; Female ; Aged ; Adult ; Adolescent ; Young Adult ; Child ; Aged, 80 and over ; Child, Preschool ; Immunodiffusion ; Infant

Comparison of galactomannan lateral flow assay and enzyme immunoassay to identify Aspergillus spp. in bronchoalveolar lavage fluid.

Autori: Martins SC Tararam CA Levy LO a ďalší

Zdroj: The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases [Braz J Infect Dis] 2024 Jul-Aug; Vol. 28 (4), pp. 103838. Date of Electronic Publication: 2024 Jul 14.

Spôsob vydávania: Journal Article; Comparative Study

Informácie o časopise: Publisher: Elsevier Editora Country of Publication: Brazil NLM ID: 9812937 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4391 (Electronic) Linking ISSN: 14138670 NLM ISO Abbreviation: Braz J Infect Dis Subsets: MEDLINE

Výrazy zo slovníka MeSH: Mannans*/analysis , Galactose*/analogs & derivatives , Bronchoalveolar Lavage Fluid*/microbiology , Bronchoalveolar Lavage Fluid*/chemistry , Aspergillus*/immunology , Aspergillus*/isolation & purification , Immunoenzyme Techniques*/methods , Sensitivity and Specificity*, Humans ; Aspergillosis/diagnosis ; Aspergillosis/microbiology ; Biomarkers/analysis ; Antigens, Fungal/analysis ; Reproducibility of Results

Evaluation of fully automated chemiluminescent enzyme immunoassays for hepatitis B core-related antigen components, phosphorylated and non-phosphorylated hepatitis B core antigens: clinical significance and dynamics during hepatitis B e antigen seroconversion.

Autori: Suzuki T Ohue C Arai O a ďalší

Zdroj: Journal of clinical microbiology [J Clin Microbiol] 2025 Sep 10; Vol. 63 (9), pp. e0038525. Date of Electronic Publication: 2025 Aug 19.

Spôsob vydávania: Journal Article; Evaluation Study

Informácie o časopise: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Hepatitis B Core Antigens*/blood , Luminescent Measurements*/methods , Hepatitis B*/diagnosis , Hepatitis B*/drug therapy , Hepatitis B*/immunology , Hepatitis B e Antigens*/blood , Hepatitis B e Antigens*/immunology , Hepatitis B, Chronic*/drug therapy , Hepatitis B, Chronic*/diagnosis, Humans ; Immunoenzyme Techniques/methods ; Seroconversion ; Male ; Female ; Adult ; Middle Aged ; Hepatitis B virus/immunology ; Automation, Laboratory/methods ; Biomarkers/blood ; Phosphorylation ; Clinical Relevance

Development of a chemiluminescence enzyme immunoassay (CLEIA) for quantitating L1 protein in HPV vaccines.

Autori: Fei C Yang H Wang S a ďalší

Zdroj: Analytical biochemistry [Anal Biochem] 2025 Sep; Vol. 704, pp. 115889. Date of Electronic Publication: 2025 May 02.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Papillomavirus Vaccines*/chemistry , Papillomavirus Vaccines*/immunology , Papillomavirus Vaccines*/analysis , Capsid Proteins*/analysis , Capsid Proteins*/immunology , Luminescent Measurements*/methods , Oncogene Proteins, Viral*/analysis , Oncogene Proteins, Viral*/immunology, Immunoenzyme Techniques/methods ; Humans

Establishment of an immunoperoxidase monolayer assay for the detection of African swine fever virus antibodies.

Autori: Yin L Wang W Liu F a ďalší

Zdroj: Journal of virological methods [J Virol Methods] 2025 Sep; Vol. 337, pp. 115173. Date of Electronic Publication: 2025 May 03.

Spôsob vydávania: Journal Article; Research Support, Non-U.S. Gov't

Informácie o časopise: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

Výrazy zo slovníka MeSH: African Swine Fever Virus*/immunology , African Swine Fever*/diagnosis , African Swine Fever*/immunology , Antibodies, Viral*/blood, Animals ; Swine ; Sensitivity and Specificity ; Immunoenzyme Techniques/methods ; China

Functional Analysis and Clinical Significance of Glutaredoxin 2 in Colon Cancer.

Autori: Shimizu H Kurashima K Fukami T a ďalší

Zdroj: Annals of surgical oncology [Ann Surg Oncol] 2025 Sep; Vol. 32 (9), pp. 7038-7048. Date of Electronic Publication: 2025 Jun 05.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Springer Country of Publication: United States NLM ID: 9420840 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1534-4681 (Electronic) Linking ISSN: 10689265 NLM ISO Abbreviation: Ann Surg Oncol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Glutaredoxins*/genetics , Glutaredoxins*/metabolism , Glutaredoxins*/antagonists & inhibitors , Colonic Neoplasms*/pathology , Colonic Neoplasms*/genetics , Colonic Neoplasms*/metabolism , Colonic Neoplasms*/mortality , Biomarkers, Tumor*/genetics , Biomarkers, Tumor*/metabolism, Humans ; Cell Proliferation ; Cell Movement ; Apoptosis ; Male ; Prognosis ; Female ; RNA, Messenger/genetics ; Survival Rate ; Middle Aged ; Tumor Cells, Cultured ; Follow-Up Studies ; Neoplasm Staging ; Cell Cycle ; Immunoenzyme Techniques ; Aged ; RNA, Small Interfering/genetics ; Gene Expression Regulation, Neoplastic ; Clinical Relevance