Search Results - "High-Throughput Screening Assays (mesh)"

From bench to biofactory: high-throughput technologies and automated workflows to accelerate biomanufacturing

Authors: Petzold, Christopher J Mukhopadhyay, Aindrila

Source: Current Opinion in Biotechnology. 94

Subject Terms: 31 Biological Sciences (for-2020), 3106 Industrial Biotechnology (for-2020), Machine Learning and Artificial Intelligence (rcdc), Bioengineering (rcdc), Biotechnology (rcdc), Networking and Information Technology R&D (NITRD) (rcdc), Workflow (mesh), Automation (mesh), Biotechnology (mesh), High-Throughput Screening Assays (mesh), Biotechnology (mesh), Automation (mesh), High-Throughput Screening Assays (mesh), Workflow (mesh), Workflow (mesh), Automation (mesh), Biotechnology (mesh), High-Throughput Screening Assays (mesh), 06 Biological Sciences (for), 09 Engineering (for), 10 Technology (for), Biotechnology (science-metrix), 3001 Agricultural biotechnology (for-2020), 3106 Industrial biotechnology (for-2020), 3206 Medical biotechnology (for-2020)

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Optimization of a micro-scale air–liquid-interface model of human proximal airway epithelium for moderate throughput drug screening for SARS-CoV-2

Authors: Sen, Chandani Rickabaugh, Tammy M Jeyachandran, Arjit Vijey et al.

Source: Respiratory Research. 26(1)

Subject Terms: 3207 Medical Microbiology (for-2020), 32 Biomedical and Clinical Sciences (for-2020), Infectious Diseases (rcdc), Stem Cell Research (rcdc), Coronaviruses (rcdc), Emerging Infectious Diseases (rcdc), Lung (rcdc), 2.1 Biological and endogenous factors (hrcs-rac), Infection (hrcs-hc), 3 Good Health and Well Being (sdg), Humans (mesh), SARS-CoV-2 (mesh), Antiviral Agents (mesh), High-Throughput Screening Assays (mesh), Drug Evaluation, Preclinical (mesh), Respiratory Mucosa (mesh), COVID-19 Drug Treatment (mesh), COVID-19 (mesh), Cells, Cultured (mesh), Human mucociliary epithelium, SARS-CoV-2, Respiratory viral infections, High throughput drug screening, Anti-viral screening, Small-molecules, Air-liquid-interface, Heterogeneity, Image quantification, RNA sequencing, Respiratory Mucosa (mesh), Cells, Cultured (mesh), Humans (mesh), Antiviral Agents (mesh), Drug Evaluation, Preclinical (mesh), High-Throughput Screening Assays (mesh), COVID-19 (mesh), SARS-CoV-2 (mesh), COVID-19 Drug Treatment (mesh), Air–liquid-interface, Anti-viral screening, Heterogeneity, High throughput drug screening, Human mucociliary epithelium, Image quantification, RNA sequencing, Respiratory viral infections, SARS-CoV-2, Small-molecules, Humans (mesh), SARS-CoV-2 (mesh), Antiviral Agents (mesh), High-Throughput Screening Assays (mesh), Drug Evaluation, Preclinical (mesh), Respiratory Mucosa (mesh), COVID-19 Drug Treatment (mesh), COVID-19 (mesh), Cells, Cultured (mesh), 1102 Cardiorespiratory Medicine and Haematology (for), 1103 Clinical Sciences (for), Respiratory System (science-metrix), 3201 Cardiovascular medicine and haematology (for-2020), 3202 Clinical sciences (for-2020)

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High-throughput genetics enables identification of nutrient utilization and accessory energy metabolism genes in a model methanogen

Authors: Day, Leslie A Carlson, Hans K Fonseca, Dallas R et al.

Source: mBio. 15(9)

Subject Terms: 3107 Microbiology (for-2020), 31 Biological Sciences (for-2020), 3103 Ecology (for-2020), Genetics (rcdc), 1.1 Normal biological development and functioning (hrcs-rac), Generic health relevance (hrcs-hc), Methanococcus (mesh), Energy Metabolism (mesh), Nitrogen (mesh), DNA Transposable Elements (mesh), Nutrients (mesh), Archaeal Proteins (mesh), High-Throughput Screening Assays (mesh), archaea, transposons, metabolism, Methanococcus (mesh), Nitrogen (mesh), Archaeal Proteins (mesh), DNA Transposable Elements (mesh), Energy Metabolism (mesh), High-Throughput Screening Assays (mesh), Nutrients (mesh), archaea, metabolism, transposons, Methanococcus (mesh), Energy Metabolism (mesh), Nitrogen (mesh), DNA Transposable Elements (mesh), Nutrients (mesh), Archaeal Proteins (mesh), High-Throughput Screening Assays (mesh), 0605 Microbiology (for), 3101 Biochemistry and cell biology (for-2020), 3107 Microbiology (for-2020), 3207 Medical microbiology (for-2020)

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Phylogenomically Guided Identification of Industrially Relevant GH1 β‑Glucosidases through DNA Synthesis and Nanostructure-Initiator Mass Spectrometry

Authors: Heins, Richard A Cheng, Xiaoliang Nath, Sangeeta et al.

Source: ACS Chemical Biology. 9(9)

Subject Terms: 3401 Analytical Chemistry (for-2020), 31 Biological Sciences (for-2020), 34 Chemical Sciences (for-2020), Biotechnology (rcdc), Generic health relevance (hrcs-hc), Biomass (mesh), Biotechnology (mesh), Cellulases (mesh), Chromatography, High Pressure Liquid (mesh), DNA (mesh), Glucans (mesh), High-Throughput Screening Assays (mesh), Hydrogen-Ion Concentration (mesh), Hydrolysis (mesh), Imidazoles (mesh), Ionic Liquids (mesh), Mass Spectrometry (mesh), Nanostructures (mesh), Phylogeny (mesh), Substrate Specificity (mesh), Temperature (mesh), Workflow (mesh), Imidazoles (mesh), Glucans (mesh), Cellulases (mesh), DNA (mesh), Chromatography, High Pressure Liquid (mesh), Biotechnology (mesh), Biomass (mesh), Temperature (mesh), Phylogeny (mesh), Substrate Specificity (mesh), Hydrolysis (mesh), Hydrogen-Ion Concentration (mesh), Nanostructures (mesh), Mass Spectrometry (mesh), Ionic Liquids (mesh), High-Throughput Screening Assays (mesh), Workflow (mesh), Biomass (mesh), Biotechnology (mesh), Cellulases (mesh), Chromatography, High Pressure Liquid (mesh), DNA (mesh), Glucans (mesh), High-Throughput Screening Assays (mesh), Hydrogen-Ion Concentration (mesh), Hydrolysis (mesh), Imidazoles (mesh), Ionic Liquids (mesh), Mass Spectrometry (mesh), Nanostructures (mesh), Phylogeny (mesh), Substrate Specificity (mesh), Temperature (mesh), Workflow (mesh), 03 Chemical Sciences (for), 06 Biological Sciences (for), Organic Chemistry (science-metrix), 31 Biological sciences (for-2020), 34 Chemical sciences (for-2020)

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Functional assays with human patient-derived enteroid monolayers to assess the human gut barrier

Authors: Sayed, Ibrahim M Tindle, Courtney Fonseca, Ayden G et al.

Source: STAR Protocols. 2(3)

Subject Terms: 3107 Microbiology (for-2020), 31 Biological Sciences (for-2020), 1.1 Normal biological development and functioning (hrcs-rac), Cell Culture Techniques (mesh), Epithelium (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Intestinal Mucosa (mesh), Intestine, Small (mesh), Models, Biological (mesh), Organoids (mesh), Primary Cell Culture (mesh), Vaping (mesh), Intestinal Mucosa (mesh), Intestine, Small (mesh), Epithelium (mesh), Organoids (mesh), Humans (mesh), Cell Culture Techniques (mesh), Models, Biological (mesh), High-Throughput Screening Assays (mesh), Primary Cell Culture (mesh), Vaping (mesh), Cell Biology, Cell culture, High Throughput Screening, Immunology, Microbiology, Organoids, Stem Cells, Cell Culture Techniques (mesh), Epithelium (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Intestinal Mucosa (mesh), Intestine, Small (mesh), Models, Biological (mesh), Organoids (mesh), Primary Cell Culture (mesh), Vaping (mesh)

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Discovery and characterization of bromodomain 2–specific inhibitors of BRDT

Authors: Yu, Zhifeng Ku, Angela F Anglin, Justin L et al.

Source: Proceedings of the National Academy of Sciences of the United States of America. 118(9)

Subject Terms: 3101 Biochemistry and Cell Biology (for-2020), 32 Biomedical and Clinical Sciences (for-2020), 3404 Medicinal and Biomolecular Chemistry (for-2020), 34 Chemical Sciences (for-2020), 31 Biological Sciences (for-2020), Contraception/Reproduction (rcdc), Cancer (rcdc), Animals (mesh), Azepines (mesh), Binding Sites (mesh), Cell Cycle Proteins (mesh), Cloning, Molecular (mesh), Contraceptive Agents, Male (mesh), Crystallography, X-Ray (mesh), Drug Discovery (mesh), Escherichia coli (mesh), Gene Expression (mesh), Genetic Vectors (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Ligands (mesh), Male (mesh), Mice (mesh), Molecular Docking Simulation (mesh), Nuclear Proteins (mesh), Protein Binding (mesh), Protein Conformation, alpha-Helical (mesh), Protein Conformation, beta-Strand (mesh), Protein Interaction Domains and Motifs (mesh), Quantitative Structure-Activity Relationship (mesh), Recombinant Proteins (mesh), Testis (mesh), Transcription Factors (mesh), Triazoles (mesh), Testis (mesh), Animals (mesh), Humans (mesh), Mice (mesh), Escherichia coli (mesh), Azepines (mesh), Triazoles (mesh), Contraceptive Agents, Male (mesh), Cell Cycle Proteins (mesh), Nuclear Proteins (mesh), Recombinant Proteins (mesh), Transcription Factors (mesh), Ligands (mesh), Crystallography, X-Ray (mesh), Cloning, Molecular (mesh), Gene Expression (mesh), Binding Sites (mesh), Protein Binding (mesh), Quantitative Structure-Activity Relationship (mesh), Genetic Vectors (mesh), Male (mesh), Protein Interaction Domains and Motifs (mesh), Drug Discovery (mesh), High-Throughput Screening Assays (mesh), Molecular Docking Simulation (mesh), Protein Conformation, alpha-Helical (mesh), Protein Conformation, beta-Strand (mesh), BET bromodomains, DNA-encoded chemistry, male contraceptive, small-molecule inhibitors, Animals (mesh), Azepines (mesh), Binding Sites (mesh), Cell Cycle Proteins (mesh), Cloning, Molecular (mesh), Contraceptive Agents, Male (mesh), Crystallography, X-Ray (mesh), Drug Discovery (mesh), Escherichia coli (mesh), Gene Expression (mesh), Genetic Vectors (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Ligands (mesh), Male (mesh), Mice (mesh), Molecular Docking Simulation (mesh), Nuclear Proteins (mesh), Protein Binding (mesh), Protein Conformation, alpha-Helical (mesh), Protein Conformation, beta-Strand (mesh), Protein Interaction Domains and Motifs (mesh), Quantitative Structure-Activity Relationship (mesh), Recombinant Proteins (mesh), Testis (mesh), Transcription Factors (mesh), Triazoles (mesh)

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Engineering Pseudomonas putida for efficient aromatic conversion to bioproduct using high throughput screening in a bioreactor

Authors: Eng, Thomas Banerjee, Deepanwita Lau, Andrew K et al.

Source: Metabolic Engineering. 66

Subject Terms: 31 Biological Sciences (for-2020), 3106 Industrial Biotechnology (for-2020), Biotechnology (rcdc), 7 Affordable and Clean Energy (sdg), Bioreactors (mesh), Carbon (mesh), Gene Library (mesh), High-Throughput Screening Assays (mesh), Pseudomonas putida (mesh), Para-coumarate, Bioreactor, RB-TnSeq, Pseudomonas putida, Metabolic engineering, Pseudomonas putida (mesh), Carbon (mesh), Bioreactors (mesh), Gene Library (mesh), High-Throughput Screening Assays (mesh), Bioreactor, Metabolic engineering, Para-coumarate, Pseudomonas putida, RB-TnSeq, Bioreactors (mesh), Carbon (mesh), Gene Library (mesh), High-Throughput Screening Assays (mesh), Pseudomonas putida (mesh), 1003 Industrial Biotechnology (for), Biotechnology (science-metrix), 3101 Biochemistry and cell biology (for-2020), 3106 Industrial biotechnology (for-2020)

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Genome-wide screens uncover KDM2B as a modifier of protein binding to heparan sulfate

Authors: Weiss, Ryan J Spahn, Philipp N Chiang, Austin WT et al.

Source: Nature Chemical Biology. 17(6)

Subject Terms: 3101 Biochemistry and Cell Biology (for-2020), 31 Biological Sciences (for-2020), Genetics (rcdc), Human Genome (rcdc), 2.1 Biological and endogenous factors (hrcs-rac), Cancer (hrcs-hc), Algorithms (mesh), CRISPR-Cas Systems (mesh), Cell Line, Tumor (mesh), Cell Proliferation (mesh), Drug Discovery (mesh), Extracellular Matrix (mesh), F-Box Proteins (mesh), Genome-Wide Association Study (mesh), Heparitin Sulfate (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Jumonji Domain-Containing Histone Demethylases (mesh), Protein Binding (mesh), RNA-Seq (mesh), Sulfotransferases (mesh), Cell Line, Tumor (mesh), Extracellular Matrix (mesh), Humans (mesh), Sulfotransferases (mesh), Heparitin Sulfate (mesh), F-Box Proteins (mesh), Cell Proliferation (mesh), Protein Binding (mesh), Algorithms (mesh), Genome-Wide Association Study (mesh), Drug Discovery (mesh), Jumonji Domain-Containing Histone Demethylases (mesh), High-Throughput Screening Assays (mesh), CRISPR-Cas Systems (mesh), RNA-Seq (mesh), Algorithms (mesh), CRISPR-Cas Systems (mesh), Cell Line, Tumor (mesh), Cell Proliferation (mesh), Drug Discovery (mesh), Extracellular Matrix (mesh), F-Box Proteins (mesh), Genome-Wide Association Study (mesh), Heparitin Sulfate (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Jumonji Domain-Containing Histone Demethylases (mesh), Protein Binding (mesh), RNA-Seq (mesh), Sulfotransferases (mesh), 0304 Medicinal and Biomolecular Chemistry (for), 0601 Biochemistry and Cell Biology (for), Biochemistry & Molecular Biology (science-metrix), 3101 Biochemistry and cell biology (for-2020), 3404 Medicinal and biomolecular chemistry (for-2020)

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Joint probabilistic modeling of single-cell multi-omic data with totalVI

Authors: Gayoso, Adam Steier, Zoë Lopez, Romain et al.

Source: Nature Methods. 18(3)

Subject Terms: 31 Biological Sciences (for-2020), 3102 Bioinformatics and Computational Biology (for-2020), Genetics (rcdc), Human Genome (rcdc), Cancer (rcdc), Cancer Genomics (rcdc), Animals (mesh), Cells, Cultured (mesh), Data Analysis (mesh), Female (mesh), High-Throughput Screening Assays (mesh), Lymph Nodes (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Proteins (mesh), RNA (mesh), Single-Cell Analysis (mesh), Spleen (mesh), Transcriptome (mesh), Lymph Nodes (mesh), Spleen (mesh), Cells, Cultured (mesh), Animals (mesh), Mice, Inbred C57BL (mesh), Mice (mesh), Proteins (mesh), RNA (mesh), Female (mesh), High-Throughput Screening Assays (mesh), Single-Cell Analysis (mesh), Transcriptome (mesh), Data Analysis (mesh), Animals (mesh), Cells, Cultured (mesh), Data Analysis (mesh), Female (mesh), High-Throughput Screening Assays (mesh), Lymph Nodes (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Proteins (mesh), RNA (mesh), Single-Cell Analysis (mesh), Spleen (mesh), Transcriptome (mesh), 06 Biological Sciences (for), 10 Technology (for), 11 Medical and Health Sciences (for), Developmental Biology (science-metrix), 31 Biological sciences (for-2020)

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Novel application of automated machine learning with MALDI-TOF-MS for rapid high-throughput screening of COVID-19: a proof of concept

Authors: Tran, Nam K Howard, Taylor Walsh, Ryan et al.

Source: Scientific Reports. 11(1)

Subject Terms: 3401 Analytical Chemistry (for-2020), 34 Chemical Sciences (for-2020), Bioengineering (rcdc), Emerging Infectious Diseases (rcdc), Coronaviruses Diagnostics and Prognostics (rcdc), Biotechnology (rcdc), Machine Learning and Artificial Intelligence (rcdc), Infectious Diseases (rcdc), Coronaviruses (rcdc), 4.1 Discovery and preclinical testing of markers and technologies (hrcs-rac), 3 Good Health and Well Being (sdg), Automation (mesh), COVID-19 (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Machine Learning (mesh), Proof of Concept Study (mesh), SARS-CoV-2 (mesh), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (mesh), Humans (mesh), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (mesh), Automation (mesh), High-Throughput Screening Assays (mesh), Machine Learning (mesh), Proof of Concept Study (mesh), COVID-19 (mesh), SARS-CoV-2 (mesh), Automation (mesh), COVID-19 (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Machine Learning (mesh), Proof of Concept Study (mesh), SARS-CoV-2 (mesh), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (mesh)

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High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways

Authors: Covarrubias, Sergio Vollmers, Apple Cortez Capili, Allyson et al.

Source: Cell Reports. 33(13)

Subject Terms: 3101 Biochemistry and Cell Biology (for-2020), 31 Biological Sciences (for-2020), Biotechnology (rcdc), Genetics (rcdc), 1.1 Normal biological development and functioning (hrcs-rac), 2.1 Biological and endogenous factors (hrcs-rac), Inflammatory and immune system (hrcs-hc), Generic health relevance (hrcs-hc), 3' Untranslated Regions (mesh), Animals (mesh), CRISPR-Cas Systems (mesh), Cell Line (mesh), Cell Survival (mesh), Clustered Regularly Interspaced Short Palindromic Repeats (mesh), Flow Cytometry (mesh), Gene Expression Regulation (mesh), HEK293 Cells (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Inflammation (mesh), Macrophages (mesh), Mice (mesh), NF-kappa B (mesh), RNA, Guide, CRISPR-Cas Systems (mesh), Signal Transduction (mesh), Tumor Necrosis Factor-alpha (mesh), Cell Line (mesh), Macrophages (mesh), Animals (mesh), Humans (mesh), Mice (mesh), Inflammation (mesh), Tumor Necrosis Factor-alpha (mesh), NF-kappa B (mesh), 3' Untranslated Regions (mesh), Flow Cytometry (mesh), Signal Transduction (mesh), Cell Survival (mesh), Gene Expression Regulation (mesh), High-Throughput Screening Assays (mesh), HEK293 Cells (mesh), CRISPR-Cas Systems (mesh), Clustered Regularly Interspaced Short Palindromic Repeats (mesh), RNA, Guide, CRISPR-Cas Systems (mesh), 3′ UTR, CRISPR screen, NF-kappaB, NF-κB, TNF, inflammation, macrophage, viability, 3' Untranslated Regions (mesh), Animals (mesh), CRISPR-Cas Systems (mesh), Cell Line (mesh), Cell Survival (mesh), Clustered Regularly Interspaced Short Palindromic Repeats (mesh), Flow Cytometry (mesh), Gene Expression Regulation (mesh), HEK293 Cells (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Inflammation (mesh), Macrophages (mesh), Mice (mesh), NF-kappa B (mesh), RNA, Guide, CRISPR-Cas Systems (mesh), Signal Transduction (mesh), Tumor Necrosis Factor-alpha (mesh), 0601 Biochemistry and Cell Biology (for), 1116 Medical Physiology (for), 31 Biological sciences (for-2020)

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High-throughput screening identifies modulators of sarcospan that stabilize muscle cells and exhibit activity in the mouse model of Duchenne muscular dystrophy

Authors: Shu, Cynthia Parfenova, Liubov Mokhonova, Ekaterina et al.

Source: Skeletal Muscle. 10(1)

Subject Terms: 3208 Medical Physiology (for-2020), 32 Biomedical and Clinical Sciences (for-2020), Orphan Drug (rcdc), Muscular Dystrophy (rcdc), Duchenne/ Becker Muscular Dystrophy (rcdc), Biotechnology (rcdc), Genetics (rcdc), Pediatric (rcdc), Rare Diseases (rcdc), 5.1 Pharmaceuticals (hrcs-rac), 2.1 Biological and endogenous factors (hrcs-rac), 5.2 Cellular and gene therapies (hrcs-rac), Musculoskeletal (hrcs-hc), Animals (mesh), Cell Line (mesh), Drug Discovery (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Male (mesh), Membrane Proteins (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Mice, Inbred mdx (mesh), Muscle Fibers, Skeletal (mesh), Muscular Dystrophy, Duchenne (mesh), Neoplasm Proteins (mesh), Small Molecule Libraries (mesh), Drug discovery, Duchenne muscular dystrophy, Dystrophin, High-throughput screen, Sarcolemma, Sarcospan, Small molecules, Cell Line (mesh), Animals (mesh), Mice, Inbred C57BL (mesh), Mice, Inbred mdx (mesh), Humans (mesh), Mice (mesh), Muscular Dystrophy, Duchenne (mesh), Membrane Proteins (mesh), Neoplasm Proteins (mesh), Male (mesh), Small Molecule Libraries (mesh), Muscle Fibers, Skeletal (mesh), Drug Discovery (mesh), High-Throughput Screening Assays (mesh), Drug discovery, Duchenne muscular dystrophy, Dystrophin, High-throughput screen, Sarcolemma, Sarcospan, Small molecules, Animals (mesh), Cell Line (mesh), Drug Discovery (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Male (mesh), Membrane Proteins (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Mice, Inbred mdx (mesh), Muscle Fibers, Skeletal (mesh), Muscular Dystrophy, Duchenne (mesh), Neoplasm Proteins (mesh), Small Molecule Libraries (mesh), 3105 Genetics (for-2020), 3208 Medical physiology (for-2020)

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High-Throughput Screening of Kawasaki Disease Sera for Antiviral Antibodies

Authors: Quiat, Daniel Kula, Tomasz Shimizu, Chisato et al.

Source: Journal of Infectious Diseases. 222(11)

Subject Terms: 3207 Medical Microbiology (for-2020), 32 Biomedical and Clinical Sciences (for-2020), Infectious Diseases (rcdc), Autoimmune Disease (rcdc), Rare Diseases (rcdc), Biotechnology (rcdc), Pediatric (rcdc), 2.1 Biological and endogenous factors (hrcs-rac), 2.2 Factors relating to the physical environment (hrcs-rac), Infection (hrcs-hc), Antibodies, Viral (mesh), Antiviral Agents (mesh), Bacteriophages (mesh), Child, Preschool (mesh), Female (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Immunoglobulin G (mesh), Infant (mesh), Male (mesh), Mucocutaneous Lymph Node Syndrome (mesh), Kawasaki disease, virus, antibody, Humans (mesh), Bacteriophages (mesh), Mucocutaneous Lymph Node Syndrome (mesh), Immunoglobulin G (mesh), Antibodies, Viral (mesh), Antiviral Agents (mesh), Child, Preschool (mesh), Infant (mesh), Female (mesh), Male (mesh), High-Throughput Screening Assays (mesh), Kawasaki disease, antibody, virus, Antibodies, Viral (mesh), Antiviral Agents (mesh), Bacteriophages (mesh), Child, Preschool (mesh), Female (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Immunoglobulin G (mesh), Infant (mesh), Male (mesh), Mucocutaneous Lymph Node Syndrome (mesh), 06 Biological Sciences (for), 11 Medical and Health Sciences (for), Microbiology (science-metrix), 31 Biological sciences (for-2020), 32 Biomedical and clinical sciences (for-2020), 42 Health sciences (for-2020)

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Transcriptional profiling and therapeutic targeting of oxidative stress in neuroinflammation

Authors: Mendiola, Andrew S Ryu, Jae Kyu Bardehle, Sophia et al.

Source: Nature Immunology. 21(5)

Subject Terms: 32 Biomedical and Clinical Sciences (for-2020), 3204 Immunology (for-2020), Human Genome (rcdc), Neurodegenerative (rcdc), Neurosciences (rcdc), Biotechnology (rcdc), Genetics (rcdc), Brain Disorders (rcdc), 2.1 Biological and endogenous factors (hrcs-rac), 5.1 Pharmaceuticals (hrcs-rac), 1.1 Normal biological development and functioning (hrcs-rac), Generic health relevance (hrcs-hc), Neurological (hrcs-hc), Animals (mesh), Antioxidants (mesh), Disease Models, Animal (mesh), Encephalomyelitis, Autoimmune, Experimental (mesh), Female (mesh), Gene Expression Profiling (mesh), Gene Regulatory Networks (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Immunity, Innate (mesh), Isoxazoles (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Mice, Mutant Strains (mesh), Microglia (mesh), Multiple Sclerosis (mesh), Neurogenic Inflammation (mesh), Oxidative Stress (mesh), Sequence Analysis, RNA (mesh), Single-Cell Analysis (mesh), Microglia (mesh), Animals (mesh), Mice, Inbred C57BL (mesh), Humans (mesh), Mice (mesh), Mice, Mutant Strains (mesh), Multiple Sclerosis (mesh), Encephalomyelitis, Autoimmune, Experimental (mesh), Neurogenic Inflammation (mesh), Disease Models, Animal (mesh), Isoxazoles (mesh), Antioxidants (mesh), Gene Expression Profiling (mesh), Sequence Analysis, RNA (mesh), Oxidative Stress (mesh), Female (mesh), Gene Regulatory Networks (mesh), Immunity, Innate (mesh), High-Throughput Screening Assays (mesh), Single-Cell Analysis (mesh), Animals (mesh), Antioxidants (mesh), Disease Models, Animal (mesh), Encephalomyelitis, Autoimmune, Experimental (mesh), Female (mesh), Gene Expression Profiling (mesh), Gene Regulatory Networks (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Immunity, Innate (mesh), Isoxazoles (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Mice, Mutant Strains (mesh), Microglia (mesh), Multiple Sclerosis (mesh), Neurogenic Inflammation (mesh), Oxidative Stress (mesh), Sequence Analysis, RNA (mesh), Single-Cell Analysis (mesh), 1107 Immunology (for), Immunology (science-metrix), 3101 Biochemistry and cell biology (for-2020), 3204 Immunology (for-2020)

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Comprehensive In Vivo Interrogation Reveals Phenotypic Impact of Human Enhancer Variants

Authors: Kvon, Evgeny Z Zhu, Yiwen Kelman, Guy et al.

Source: Cell. 180(6)

Subject Terms: 31 Biological Sciences (for-2020), 32 Biomedical and Clinical Sciences (for-2020), 3105 Genetics (for-2020), Genetics (rcdc), Human Genome (rcdc), 2.1 Biological and endogenous factors (hrcs-rac), Animals (mesh), Enhancer Elements, Genetic (mesh), Gene Expression Regulation, Developmental (mesh), Gene Knock-In Techniques (mesh), Hedgehog Proteins (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Mice (mesh), Mutation (mesh), Phenotype (mesh), Polydactyly (mesh), RNA, Untranslated (mesh), Animals (mesh), Humans (mesh), Mice (mesh), Polydactyly (mesh), RNA, Untranslated (mesh), Gene Expression Regulation, Developmental (mesh), Phenotype (mesh), Mutation (mesh), Hedgehog Proteins (mesh), Enhancer Elements, Genetic (mesh), Gene Knock-In Techniques (mesh), High-Throughput Screening Assays (mesh), CRISPR/Cas9, Sonic hedgehog, ZRS, cis-regulatory element, enhancer, genome editing, limb development, mutation, polydactyly, rare non-coding variant, Animals (mesh), Enhancer Elements, Genetic (mesh), Gene Expression Regulation, Developmental (mesh), Gene Knock-In Techniques (mesh), Hedgehog Proteins (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Mice (mesh), Mutation (mesh), Phenotype (mesh), Polydactyly (mesh), RNA, Untranslated (mesh), 06 Biological Sciences (for), 11 Medical and Health Sciences (for), Developmental Biology (science-metrix), 31 Biological sciences (for-2020), 32 Biomedical and clinical sciences (for-2020)

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High-Throughput Drug Screening Identifies a Potent Wnt Inhibitor that Promotes Airway Basal Stem Cell Homeostasis

Authors: Aros, Cody J Paul, Manash K Pantoja, Carla J et al.

Source: Cell Reports. 30(7)

Subject Terms: 3101 Biochemistry and Cell Biology (for-2020), 31 Biological Sciences (for-2020), Stem Cell Research (rcdc), Lung (rcdc), Regenerative Medicine (rcdc), Lung Cancer (rcdc), Cancer (rcdc), 5.1 Pharmaceuticals (hrcs-rac), 1.1 Normal biological development and functioning (hrcs-rac), Cancer (hrcs-hc), Animals (mesh), Bronchi (mesh), Cell Differentiation (mesh), Drug Evaluation, Preclinical (mesh), Female (mesh), High-Throughput Screening Assays (mesh), Homeostasis (mesh), Humans (mesh), Lung (mesh), Male (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Precancerous Conditions (mesh), Small Molecule Libraries (mesh), Stem Cells (mesh), Transfection (mesh), Wnt Proteins (mesh), Wnt Signaling Pathway (mesh), beta Catenin (mesh), Lung (mesh), Bronchi (mesh), Stem Cells (mesh), Animals (mesh), Mice, Inbred C57BL (mesh), Humans (mesh), Mice (mesh), Precancerous Conditions (mesh), Drug Evaluation, Preclinical (mesh), Transfection (mesh), Cell Differentiation (mesh), Homeostasis (mesh), Female (mesh), Male (mesh), Wnt Proteins (mesh), beta Catenin (mesh), Small Molecule Libraries (mesh), High-Throughput Screening Assays (mesh), Wnt Signaling Pathway (mesh), Wnt, airway stem cell, beta-catenin, drug screen, homeostasis, lung, premalignancy, Animals (mesh), Bronchi (mesh), Cell Differentiation (mesh), Drug Evaluation, Preclinical (mesh), Female (mesh), High-Throughput Screening Assays (mesh), Homeostasis (mesh), Humans (mesh), Lung (mesh), Male (mesh), Mice (mesh), Mice, Inbred C57BL (mesh), Precancerous Conditions (mesh), Small Molecule Libraries (mesh), Stem Cells (mesh), Transfection (mesh), Wnt Proteins (mesh), Wnt Signaling Pathway (mesh), beta Catenin (mesh), Homeostasis, drug screen, Wnt, beta-catenin, lung, premalignancy, 0601 Biochemistry and Cell Biology (for), 1116 Medical Physiology (for), 31 Biological sciences (for-2020)

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A stem cell reporter based platform to identify and target drug resistant stem cells in myeloid leukemia

Authors: Spinler, Kyle Bajaj, Jeevisha Ito, Takahiro et al.

Source: Nature Communications. 11(1)

Subject Terms: 3101 Biochemistry and Cell Biology (for-2020), 32 Biomedical and Clinical Sciences (for-2020), 31 Biological Sciences (for-2020), Hematology (rcdc), Biotechnology (rcdc), Rare Diseases (rcdc), Stem Cell Research - Nonembryonic - Non-Human (rcdc), Cancer (rcdc), Stem Cell Research (rcdc), 1.1 Normal biological development and functioning (hrcs-rac), 2.1 Biological and endogenous factors (hrcs-rac), Animals (mesh), Antineoplastic Agents (mesh), Blast Crisis (mesh), Chemoradiotherapy (mesh), Disease Models, Animal (mesh), Drug Resistance, Neoplasm (mesh), Gene Knock-In Techniques (mesh), Gene Knockout Techniques (mesh), Genes, Reporter (mesh), Green Fluorescent Proteins (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Imatinib Mesylate (mesh), Integrin beta Chains (mesh), Leukemia, Myeloid, Acute (mesh), Mice, Transgenic (mesh), Neoplastic Stem Cells (mesh), RNA-Binding Proteins (mesh), RNA-Seq (mesh), Signal Transduction (mesh), Syndecan-1 (mesh), Animals (mesh), Mice, Transgenic (mesh), Humans (mesh), Blast Crisis (mesh), Disease Models, Animal (mesh), RNA-Binding Proteins (mesh), Green Fluorescent Proteins (mesh), Integrin beta Chains (mesh), Antineoplastic Agents (mesh), Signal Transduction (mesh), Drug Resistance, Neoplasm (mesh), Genes, Reporter (mesh), Syndecan-1 (mesh), Neoplastic Stem Cells (mesh), Leukemia, Myeloid, Acute (mesh), Gene Knockout Techniques (mesh), Gene Knock-In Techniques (mesh), High-Throughput Screening Assays (mesh), Chemoradiotherapy (mesh), Imatinib Mesylate (mesh), RNA-Seq (mesh), Animals (mesh), Antineoplastic Agents (mesh), Blast Crisis (mesh), Chemoradiotherapy (mesh), Disease Models, Animal (mesh), Drug Resistance, Neoplasm (mesh), Gene Knock-In Techniques (mesh), Gene Knockout Techniques (mesh), Genes, Reporter (mesh), Green Fluorescent Proteins (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Imatinib Mesylate (mesh), Integrin beta Chains (mesh), Leukemia, Myeloid, Acute (mesh), Mice, Transgenic (mesh), Neoplastic Stem Cells (mesh), RNA-Binding Proteins (mesh), RNA-Seq (mesh), Signal Transduction (mesh), Syndecan-1 (mesh)

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Probe-target hybridization depends on spatial uniformity of initial concentration condition across large-format chips

Authors: Geldert, Alisha Huang, Haiyan Herr, Amy E

Source: Scientific Reports. 10(1)

Subject Terms: 3101 Biochemistry and Cell Biology (for-2020), 51 Physical Sciences (for-2020), 31 Biological Sciences (for-2020), Biotechnology (rcdc), Bioengineering (rcdc), Antibody Affinity (mesh), Antigen-Antibody Reactions (mesh), Brain Neoplasms (mesh), Cell Line, Tumor (mesh), Dose-Response Relationship, Drug (mesh), Dose-Response Relationship, Immunologic (mesh), Glioblastoma (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Hydrogels (mesh), Immunoassay (mesh), Immunoblotting (mesh), Immunohistochemistry (mesh), Nucleic Acid Hybridization (mesh), Protein Array Analysis (mesh), Single-Cell Analysis (mesh), Cell Line, Tumor (mesh), Humans (mesh), Glioblastoma (mesh), Brain Neoplasms (mesh), Hydrogels (mesh), Immunoassay (mesh), Immunoblotting (mesh), Protein Array Analysis (mesh), Immunohistochemistry (mesh), Nucleic Acid Hybridization (mesh), Antigen-Antibody Reactions (mesh), Antibody Affinity (mesh), Dose-Response Relationship, Immunologic (mesh), Dose-Response Relationship, Drug (mesh), High-Throughput Screening Assays (mesh), Single-Cell Analysis (mesh), Antibody Affinity (mesh), Antigen-Antibody Reactions (mesh), Brain Neoplasms (mesh), Cell Line, Tumor (mesh), Dose-Response Relationship, Drug (mesh), Dose-Response Relationship, Immunologic (mesh), Glioblastoma (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Hydrogels (mesh), Immunoassay (mesh), Immunoblotting (mesh), Immunohistochemistry (mesh), Nucleic Acid Hybridization (mesh), Protein Array Analysis (mesh), Single-Cell Analysis (mesh)

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Development of a high-throughput screen to identify small molecule enhancers of sarcospan for the treatment of Duchenne muscular dystrophy

Authors: Shu, Cynthia Kaxon-Rupp, Ariana N Collado, Judd R et al.

Source: Skeletal Muscle. 9(1)

Subject Terms: 3101 Biochemistry and Cell Biology (for-2020), 3208 Medical Physiology (for-2020), 32 Biomedical and Clinical Sciences (for-2020), 31 Biological Sciences (for-2020), Muscular Dystrophy (rcdc), Duchenne/ Becker Muscular Dystrophy (rcdc), Biotechnology (rcdc), Orphan Drug (rcdc), Rare Diseases (rcdc), Pediatric (rcdc), Genetics (rcdc), 5.1 Pharmaceuticals (hrcs-rac), 2.1 Biological and endogenous factors (hrcs-rac), Musculoskeletal (hrcs-hc), Animals (mesh), Cell Line (mesh), Drug Discovery (mesh), Genes, Reporter (mesh), Green Fluorescent Proteins (mesh), High-Throughput Screening Assays (mesh), Membrane Proteins (mesh), Mice (mesh), Muscular Dystrophy, Duchenne (mesh), Myoblasts (mesh), Neoplasm Proteins (mesh), Promoter Regions, Genetic (mesh), Small Molecule Libraries (mesh), Drug discovery, Duchenne muscular dystrophy, Dystrophin, Felodipine, High-throughput screen, Sarcolemma, Sarcospan, Small molecules, Cell Line (mesh), Myoblasts (mesh), Animals (mesh), Mice (mesh), Muscular Dystrophy, Duchenne (mesh), Green Fluorescent Proteins (mesh), Membrane Proteins (mesh), Neoplasm Proteins (mesh), Genes, Reporter (mesh), Small Molecule Libraries (mesh), Promoter Regions, Genetic (mesh), Drug Discovery (mesh), High-Throughput Screening Assays (mesh), C2C12, Drug discovery, Duchenne muscular dystrophy, Dystrophin, Felodipine, High-throughput screen, Sarcolemma, Sarcospan, Small molecules, Animals (mesh), Cell Line (mesh), Drug Discovery (mesh), Genes, Reporter (mesh), Green Fluorescent Proteins (mesh), High-Throughput Screening Assays (mesh), Membrane Proteins (mesh), Mice (mesh), Muscular Dystrophy, Duchenne (mesh), Myoblasts (mesh), Neoplasm Proteins (mesh), Promoter Regions, Genetic (mesh), Small Molecule Libraries (mesh), 3105 Genetics (for-2020), 3208 Medical physiology (for-2020)

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Adaptive Resistance to Dual BRAF/MEK Inhibition in BRAF-Driven Tumors through Autocrine FGFR Pathway Activation

Authors: Wang, Victoria E Xue, Jenny Y Frederick, Dennie T et al.

Source: Clinical Cancer Research. 25(23)

Subject Terms: 32 Biomedical and Clinical Sciences (for-2020), 3211 Oncology and Carcinogenesis (for-2020), Clinical Research (rcdc), Cancer (rcdc), 5.1 Pharmaceuticals (hrcs-rac), Cancer (hrcs-hc), Animals (mesh), Antineoplastic Agents (mesh), Apoptosis (mesh), Autocrine Communication (mesh), Biomarkers, Tumor (mesh), Carcinoma, Non-Small-Cell Lung (mesh), Cell Proliferation (mesh), Drug Resistance, Neoplasm (mesh), Fibroblast Growth Factor 1 (mesh), Gene Expression Regulation, Neoplastic (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Lung Neoplasms (mesh), MAP Kinase Kinase 1 (mesh), Melanoma (mesh), Mice (mesh), Mice, Nude (mesh), Prognosis (mesh), Proto-Oncogene Proteins B-raf (mesh), Receptor, Fibroblast Growth Factor, Type 1 (mesh), Survival Rate (mesh), Tumor Cells, Cultured (mesh), Xenograft Model Antitumor Assays (mesh), Tumor Cells, Cultured (mesh), Animals (mesh), Humans (mesh), Mice (mesh), Mice, Nude (mesh), Melanoma (mesh), Carcinoma, Non-Small-Cell Lung (mesh), Lung Neoplasms (mesh), Proto-Oncogene Proteins B-raf (mesh), MAP Kinase Kinase 1 (mesh), Fibroblast Growth Factor 1 (mesh), Antineoplastic Agents (mesh), Prognosis (mesh), Survival Rate (mesh), Xenograft Model Antitumor Assays (mesh), Autocrine Communication (mesh), Apoptosis (mesh), Cell Proliferation (mesh), Gene Expression Regulation, Neoplastic (mesh), Drug Resistance, Neoplasm (mesh), Receptor, Fibroblast Growth Factor, Type 1 (mesh), High-Throughput Screening Assays (mesh), Biomarkers, Tumor (mesh), Animals (mesh), Antineoplastic Agents (mesh), Apoptosis (mesh), Autocrine Communication (mesh), Biomarkers, Tumor (mesh), Carcinoma, Non-Small-Cell Lung (mesh), Cell Proliferation (mesh), Drug Resistance, Neoplasm (mesh), Fibroblast Growth Factor 1 (mesh), Gene Expression Regulation, Neoplastic (mesh), High-Throughput Screening Assays (mesh), Humans (mesh), Lung Neoplasms (mesh), MAP Kinase Kinase 1 (mesh), Melanoma (mesh), Mice (mesh), Mice, Nude (mesh), Prognosis (mesh), Proto-Oncogene Proteins B-raf (mesh), Receptor, Fibroblast Growth Factor, Type 1 (mesh), Survival Rate (mesh), Tumor Cells, Cultured (mesh), Xenograft Model Antitumor Assays (mesh), 1112 Oncology and Carcinogenesis (for), Oncology & Carcinogenesis (science-metrix), 3202 Clinical sciences (for-2020), 3211 Oncology and carcinogenesis (for-2020)

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