Výsledky vyhľadávania - "HEK293 Cells"

Heterogeneity in an adeno-associated virus transfection-based production process limits the production efficiency.

Autori: Ladd B Tunmats S Gräslund T a ďalší

Zdroj: Scientific reports [Sci Rep] 2025 Nov 04; Vol. 15 (1), pp. 38459. Date of Electronic Publication: 2025 Nov 04.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Výrazy zo slovníka MeSH: Dependovirus*/genetics , Transfection*/methods , Genetic Vectors*/genetics, Humans ; HEK293 Cells ; Plasmids/genetics ; Genetic Therapy/methods ; Polyethyleneimine/chemistry

Spectral Profiling of Early αsyn Aggregation in HEK293 Cells Modified to Stably Express Human WT and A53T-αsyn.

Autori: Swaminathan P Godø KS Bjørn EB a ďalší

Zdroj: Cells [Cells] 2025 Oct 02; Vol. 14 (19). Date of Electronic Publication: 2025 Oct 02.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE

Výrazy zo slovníka MeSH: alpha-Synuclein*/metabolism , alpha-Synuclein*/genetics , alpha-Synuclein*/chemistry , Protein Aggregates* , Protein Aggregation, Pathological*/metabolism, Humans ; HEK293 Cells ; Mutation/genetics

Enhanced Adeno-Associated Virus Production in HEK293 Cells via Dual Inducible Control of Cap and Rep Proteins.

Autori: Ren H Nie J Song Z a ďalší

Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2025 Oct; Vol. 122 (10), pp. 2862-2873. Date of Electronic Publication: 2025 Jun 30.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0290 (Electronic) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE

Výrazy zo slovníka MeSH: Dependovirus*/genetics , Dependovirus*/growth & development , Viral Proteins*/genetics , Viral Proteins*/metabolism , Genetic Vectors*/genetics, Humans ; HEK293 Cells ; Promoter Regions, Genetic ; Virus Replication

From simulation to immunization: efficient non-viral delivery of HIV-1 Nef ^mut -Tat DNA construct using CM18-TAT11 cell penetrating peptide.

Autori: Gheydi M Bolhassani A Negahdari B a ďalší

Zdroj: International journal of pharmaceutics [Int J Pharm] 2025 Dec 25; Vol. 686, pp. 126334. Date of Electronic Publication: 2025 Nov 01.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE

Výrazy zo slovníka MeSH: Cell-Penetrating Peptides*/administration & dosage , Cell-Penetrating Peptides*/chemistry , Vaccines, DNA*/administration & dosage , Vaccines, DNA*/immunology , nef Gene Products, Human Immunodeficiency Virus*/genetics , nef Gene Products, Human Immunodeficiency Virus*/immunology , nef Gene Products, Human Immunodeficiency Virus*/administration & dosage , AIDS Vaccines*/administration & dosage , AIDS Vaccines*/immunology , tat Gene Products, Human Immunodeficiency Virus*/genetics , tat Gene Products, Human Immunodeficiency Virus*/immunology , tat Gene Products, Human Immunodeficiency Virus*/administration & dosage, Animals ; Humans ; Molecular Dynamics Simulation ; Mice ; Mice, Inbred BALB C ; HEK293 Cells ; Female ; HIV-1/genetics ; HIV-1/immunology ; Immunization ; Nanoparticles/chemistry ; Transfection ; T-Lymphocytes, Cytotoxic/immunology

A human genome wide screen identifies miRNAs to increase recombinant Adeno-associated virus production in HEK293 cells.

Autori: Burkhart M Finkbeiner B Röscheise J a ďalší

Zdroj: New biotechnology [N Biotechnol] 2025 Dec 25; Vol. 90, pp. 268-280. Date of Electronic Publication: 2025 Nov 01.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101465345 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4347 (Electronic) Linking ISSN: 18716784 NLM ISO Abbreviation: N Biotechnol Subsets: MEDLINE

Výrazy zo slovníka MeSH: MicroRNAs*/genetics , MicroRNAs*/metabolism , Dependovirus*/genetics , Genome, Human*/genetics, Humans ; HEK293 Cells ; Genetic Vectors/genetics

Impact of the compensation effect on the production of recombinant CD81-functionalized HIV-1 Gag virus-like particles and extracellular vesicles in HEK293 cells.

Autori: García-Trujillo M Hernandez SX Gòdia F a ďalší

Zdroj: New biotechnology [N Biotechnol] 2025 Dec 25; Vol. 90, pp. 298-312. Date of Electronic Publication: 2025 Oct 30.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101465345 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4347 (Electronic) Linking ISSN: 18716784 NLM ISO Abbreviation: N Biotechnol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Tetraspanin 28*/metabolism , Tetraspanin 28*/genetics , HIV-1*/metabolism , Extracellular Vesicles*/metabolism , gag Gene Products, Human Immunodeficiency Virus*/metabolism , gag Gene Products, Human Immunodeficiency Virus*/genetics , Virion*/metabolism, Humans ; HEK293 Cells ; Recombinant Proteins/metabolism ; Green Fluorescent Proteins/metabolism

A newly synthesized compound induces apoptosis in prostate cancer cells by targeting mitochondrial dysfunction and the TP53/BCL-2/SEMA3 pathways.

Autori: Meriç N Kar E Kar F

Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2025 Dec 15; Vol. 792, pp. 152980. Date of Electronic Publication: 2025 Nov 16.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE

Výrazy zo slovníka MeSH: Apoptosis*/drug effects , Prostatic Neoplasms*/metabolism , Prostatic Neoplasms*/pathology , Prostatic Neoplasms*/drug therapy , Mitochondria*/drug effects , Mitochondria*/metabolism , Mitochondria*/pathology , Tumor Suppressor Protein p53*/metabolism , Proto-Oncogene Proteins c-bcl-2*/metabolism , Thiazoles*/pharmacology , Thiazoles*/chemistry , Thiazoles*/chemical synthesis , Antineoplastic Agents*/pharmacology , Antineoplastic Agents*/chemistry , Antineoplastic Agents*/chemical synthesis, Humans ; Male ; Signal Transduction/drug effects ; Cell Line, Tumor ; HEK293 Cells ; Cell Survival/drug effects ; PC-3 Cells

18β-glycyrrhetinic acid-amantadine hybrid: Synthesis and anti-EMCV activity via NF-κB modulation.

Autori: Xue W Zhang T Wen Y a ďalší

Zdroj: Bioorganic & medicinal chemistry [Bioorg Med Chem] 2025 Dec 15; Vol. 131, pp. 118431. Date of Electronic Publication: 2025 Oct 03.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Science Country of Publication: England NLM ID: 9413298 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-3391 (Electronic) Linking ISSN: 09680896 NLM ISO Abbreviation: Bioorg Med Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Glycyrrhetinic Acid*/chemistry , Glycyrrhetinic Acid*/pharmacology , Glycyrrhetinic Acid*/analogs & derivatives , Glycyrrhetinic Acid*/chemical synthesis , Antiviral Agents*/pharmacology , Antiviral Agents*/chemical synthesis , Antiviral Agents*/chemistry , NF-kappa B*/metabolism , NF-kappa B*/antagonists & inhibitors , Encephalomyocarditis virus*/drug effects , Amantadine*/chemistry , Amantadine*/pharmacology , Amantadine*/chemical synthesis, Humans ; Animals ; Mice ; HEK293 Cells ; Structure-Activity Relationship ; Molecular Structure ; Dose-Response Relationship, Drug ; Microbial Sensitivity Tests

Decoding bitter peptides in soy sauce: From identification to mechanism in bitter taste formation.

Autori: Bi Y Sun X Huang Y a ďalší

Zdroj: Food chemistry [Food Chem] 2025 Dec 15; Vol. 495 (Pt 2), pp. 146470. Date of Electronic Publication: 2025 Sep 22.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Soy Foods*/analysis , Peptides*/chemistry , Peptides*/metabolism , Flavoring Agents*/chemistry , Flavoring Agents*/metabolism, Taste ; Humans ; HEK293 Cells ; Molecular Docking Simulation ; Receptors, G-Protein-Coupled/metabolism ; Receptors, G-Protein-Coupled/chemistry ; Receptors, G-Protein-Coupled/genetics ; Tandem Mass Spectrometry

Targeting fungal biofilms: design, synthesis, biological and in silico studies of novel N-(5-undecyl-1,3,4-oxadiazol-2-yl)benzamide derivatives against Candida albicans.

Autori: Kumar AC Madalambika Bharathkumar PM a ďalší

Zdroj: Bioorganic & medicinal chemistry [Bioorg Med Chem] 2025 Dec 15; Vol. 131, pp. 118425. Date of Electronic Publication: 2025 Sep 29.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Science Country of Publication: England NLM ID: 9413298 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-3391 (Electronic) Linking ISSN: 09680896 NLM ISO Abbreviation: Bioorg Med Chem Subsets: MEDLINE

Výrazy zo slovníka MeSH: Biofilms*/drug effects , Candida albicans*/drug effects , Candida albicans*/physiology , Antifungal Agents*/pharmacology , Antifungal Agents*/chemical synthesis , Antifungal Agents*/chemistry , Drug Design* , Benzamides*/pharmacology , Benzamides*/chemistry , Benzamides*/chemical synthesis , Oxadiazoles*/pharmacology , Oxadiazoles*/chemistry , Oxadiazoles*/chemical synthesis, Microbial Sensitivity Tests ; Structure-Activity Relationship ; Humans ; Molecular Docking Simulation ; Molecular Structure ; Dose-Response Relationship, Drug ; HEK293 Cells

Microflow LC-MS/MS reveals platform-specific post-translational modification signatures in recombinant adeno-associated virus capsids linked to enhanced potency and stability: A quality control framework for gene therapy.

Autori: Jin J Wang W Gao T a ďalší

Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2025 Dec 15; Vol. 266, pp. 117112. Date of Electronic Publication: 2025 Aug 13.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Science Country of Publication: England NLM ID: 8309336 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-264X (Electronic) Linking ISSN: 07317085 NLM ISO Abbreviation: J Pharm Biomed Anal Subsets: MEDLINE

Výrazy zo slovníka MeSH: Dependovirus*/genetics , Dependovirus*/metabolism , Protein Processing, Post-Translational* , Capsid*/metabolism , Capsid Proteins*/metabolism , Capsid Proteins*/genetics, Humans ; Tandem Mass Spectrometry/methods ; HEK293 Cells ; Quality Control ; Genetic Therapy/methods ; Genetic Vectors/genetics ; Chromatography, Liquid/methods ; Animals ; Sf9 Cells ; Liquid Chromatography-Mass Spectrometry

TRIM52 Ubiquitin Ligase Acts as a Key Recognition Component of the Mammalian fMet/N-degron Pathway.

Autori: Kim D Park J Kim SY a ďalší

Zdroj: Journal of molecular biology [J Mol Biol] 2025 Dec 15; Vol. 437 (24), pp. 169453. Date of Electronic Publication: 2025 Sep 19.

Spôsob vydávania: Journal Article; Review

Informácie o časopise: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2985088R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1089-8638 (Electronic) Linking ISSN: 00222836 NLM ISO Abbreviation: J Mol Biol Subsets: MEDLINE

Výrazy zo slovníka MeSH: Ubiquitin-Protein Ligases*/metabolism , Ubiquitin-Protein Ligases*/genetics , Ubiquitin-Protein Ligases*/chemistry, Humans ; Animals ; Proteolysis ; HEK293 Cells ; Models, Molecular ; Apoptosis ; Degrons

Study on real time response of live cells to lentivirus infection monitored by Raman spectroscopy.

Autori: Elkhaira R Iwasaki K Sato H

Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2025 Dec 15; Vol. 343, pp. 126535. Date of Electronic Publication: 2025 Jun 03.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Country of Publication: England NLM ID: 9602533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3557 (Electronic) Linking ISSN: 13861425 NLM ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Subsets: MEDLINE

Výrazy zo slovníka MeSH: Spectrum Analysis, Raman*/methods , Lentivirus*/physiology, Humans ; HEK293 Cells ; Principal Component Analysis ; Green Fluorescent Proteins/metabolism ; Green Fluorescent Proteins/genetics

Design, synthesis and anti-influenza A virus activity of N-containing heterocyclic glycyrrhetinic acid derivatives.

Autori: Zhang J Mi L Wang Z a ďalší

Zdroj: Bioorganic & medicinal chemistry letters [Bioorg Med Chem Lett] 2025 Dec 15; Vol. 129, pp. 130364. Date of Electronic Publication: 2025 Aug 07.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9107377 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-3405 (Electronic) Linking ISSN: 0960894X NLM ISO Abbreviation: Bioorg Med Chem Lett Subsets: MEDLINE

Výrazy zo slovníka MeSH: Antiviral Agents*/pharmacology , Antiviral Agents*/chemical synthesis , Antiviral Agents*/chemistry , Glycyrrhetinic Acid*/pharmacology , Glycyrrhetinic Acid*/chemical synthesis , Glycyrrhetinic Acid*/chemistry , Glycyrrhetinic Acid*/analogs & derivatives , Drug Design* , Influenza A Virus, H1N1 Subtype*/drug effects , Heterocyclic Compounds*/chemistry , Heterocyclic Compounds*/pharmacology , Heterocyclic Compounds*/chemical synthesis, Humans ; Structure-Activity Relationship ; Molecular Docking Simulation ; Neuraminidase/metabolism ; Neuraminidase/antagonists & inhibitors ; Molecular Structure ; Microbial Sensitivity Tests ; Dose-Response Relationship, Drug ; HEK293 Cells

Synthesis and biological evaluation of novel nitrogen-containing heterocyclic derivatives as potential anticancer agents.

Autori: Li Q Li M Xiao Y a ďalší

Zdroj: Bioorganic & medicinal chemistry letters [Bioorg Med Chem Lett] 2025 Dec 15; Vol. 129, pp. 130388. Date of Electronic Publication: 2025 Aug 26.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9107377 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1464-3405 (Electronic) Linking ISSN: 0960894X NLM ISO Abbreviation: Bioorg Med Chem Lett Subsets: MEDLINE

Výrazy zo slovníka MeSH: Antineoplastic Agents*/chemical synthesis , Antineoplastic Agents*/pharmacology , Antineoplastic Agents*/chemistry , Heterocyclic Compounds*/chemistry , Heterocyclic Compounds*/chemical synthesis , Heterocyclic Compounds*/pharmacology , Nitrogen*/chemistry, Humans ; Drug Screening Assays, Antitumor ; Cell Proliferation/drug effects ; Structure-Activity Relationship ; Cell Line, Tumor ; HEK293 Cells ; Molecular Structure ; Proto-Oncogene Proteins c-akt/metabolism ; Dose-Response Relationship, Drug

Thiourea-modified poly(l-lysine) carriers with multiple hydrogen bonding interactions for safe and robust mRNA vaccine therapy.

Autori: Long J Ma S Wan R a ďalší

Zdroj: Journal of controlled release : official journal of the Controlled Release Society [J Control Release] 2025 Dec 10; Vol. 388 (Pt 2), pp. 114371. Date of Electronic Publication: 2025 Oct 29.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8607908 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4995 (Electronic) Linking ISSN: 01683659 NLM ISO Abbreviation: J Control Release Subsets: MEDLINE

Výrazy zo slovníka MeSH: Polylysine*/chemistry , Polylysine*/administration & dosage , Thiourea*/chemistry , Thiourea*/administration & dosage , RNA, Messenger*/administration & dosage , mRNA Vaccines*/administration & dosage , mRNA Vaccines*/chemistry , Drug Carriers*/chemistry , Drug Carriers*/administration & dosage , Cancer Vaccines*/administration & dosage, Animals ; Humans ; Mice ; Hydrogen Bonding ; HEK293 Cells ; RAW 264.7 Cells ; Mice, Inbred C57BL ; Transfection ; Melanoma, Experimental/therapy ; Female

The human endogenous retroviral envelope HEMO protein interacts with BACE2: Novel partnership acquired in the primate lineage.

Autori: Béguin A Chasseriaud M Hollaender G a ďalší

Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2025 Dec 09; Vol. 122 (49), pp. e2515527122. Date of Electronic Publication: 2025 Dec 02.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE

Výrazy zo slovníka MeSH: Endogenous Retroviruses*/genetics , Endogenous Retroviruses*/metabolism , Aspartic Acid Endopeptidases*/metabolism , Aspartic Acid Endopeptidases*/genetics , Gene Products, env*/metabolism , Gene Products, env*/genetics , Viral Envelope Proteins*/metabolism , Viral Envelope Proteins*/genetics, Humans ; Animals ; Primates/genetics ; HEK293 Cells ; Female ; Evolution, Molecular ; Pregnancy ; Furin/metabolism

Characterization of p53 p.T253I as a pathogenic mutation underlying Li-Fraumeni Syndrome.

Autori: Holcomb NC Harrington AM Pu H a ďalší

Zdroj: PloS one [PLoS One] 2025 Dec 05; Vol. 20 (12), pp. e0320036. Date of Electronic Publication: 2025 Dec 05 (Print Publication: 2025).

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Výrazy zo slovníka MeSH: Li-Fraumeni Syndrome*/genetics , Tumor Suppressor Protein p53*/genetics , Tumor Suppressor Protein p53*/metabolism , Adrenocortical Carcinoma*/genetics , Mutation*, Humans ; HEK293 Cells ; Proto-Oncogene Proteins c-mdm2/metabolism ; Proto-Oncogene Proteins c-mdm2/genetics ; Adrenal Cortex Neoplasms/genetics ; DNA Damage ; Female ; Child ; Male ; Germ-Line Mutation

Programmable multistep CRISPR gene activation via control of RNA polymerase III termination.

Autori: Puppala AK Nielsen AC Regan M a ďalší

Zdroj: Science advances [Sci Adv] 2025 Dec 05; Vol. 11 (49), pp. eadt1532. Date of Electronic Publication: 2025 Dec 05.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE

Výrazy zo slovníka MeSH: RNA Polymerase III*/metabolism , RNA Polymerase III*/genetics , CRISPR-Cas Systems* , Transcriptional Activation* , Clustered Regularly Interspaced Short Palindromic Repeats*/genetics, Humans ; Gene Editing/methods ; Induced Pluripotent Stem Cells/metabolism ; Induced Pluripotent Stem Cells/cytology ; HEK293 Cells

Proteome-wide computational analyses reveal links between protein condensate formation and RNA biology.

Autori: Maiti S Tripathi S Baggett DW a ďalší

Zdroj: Science advances [Sci Adv] 2025 Dec 05; Vol. 11 (49), pp. eady1420. Date of Electronic Publication: 2025 Dec 03.

Spôsob vydávania: Journal Article

Informácie o časopise: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE

Výrazy zo slovníka MeSH: Proteome*/metabolism , Computational Biology*/methods , RNA*/metabolism , RNA*/genetics , Intrinsically Disordered Proteins*/metabolism , Intrinsically Disordered Proteins*/chemistry , Intrinsically Disordered Proteins*/genetics , Biomolecular Condensates*/metabolism , Biomolecular Condensates*/chemistry, Humans ; HEK293 Cells ; Machine Learning