Search Results - "Gene Library"

Expanding the quorum sensing toolbox: Promoter libraries and hybrid promoter for dynamic genetic circuits.

Authors: De Baets J Parmentier I De Paepe B et al.

Source: New biotechnology [N Biotechnol] 2025 Dec 25; Vol. 90, pp. 97-113. Date of Electronic Publication: 2025 Sep 12.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101465345 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4347 (Electronic) Linking ISSN: 18716784 NLM ISO Abbreviation: N Biotechnol Subsets: MEDLINE

MeSH Terms: Quorum Sensing*/genetics , Promoter Regions, Genetic*/genetics , Gene Regulatory Networks*/genetics , Gene Library*, Synthetic Biology ; Gene Expression Regulation, Bacterial

Recombinant production of silk fibroin in Nicotiana benthamiana using a modular library for transgene assembly.

Authors: Bitar L Jansing J Isella B et al.

Source: New biotechnology [N Biotechnol] 2025 Dec 25; Vol. 90, pp. 313-326. Date of Electronic Publication: 2025 Nov 04.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101465345 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-4347 (Electronic) Linking ISSN: 18716784 NLM ISO Abbreviation: N Biotechnol Subsets: MEDLINE

MeSH Terms: Fibroins*/genetics , Fibroins*/biosynthesis , Nicotiana*/genetics , Nicotiana*/metabolism , Recombinant Proteins*/biosynthesis , Recombinant Proteins*/genetics , Transgenes*/genetics , Gene Library*, Plants, Genetically Modified ; Animals ; Cloning, Molecular ; Bombyx/genetics ; Silk/genetics

A machine learning-driven robotic system for autonomous nucleic acid extraction and library preparation.

Authors: Lu J Zhang Z Zhang S et al.

Source: SLAS technology [SLAS Technol] 2025 Dec; Vol. 35, pp. 100370. Date of Electronic Publication: 2025 Nov 20.

Publication Type: Journal Article

Journal Info: Publisher: SAGE Publications Country of Publication: United States NLM ID: 101697564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2472-6311 (Electronic) Linking ISSN: 24726303 NLM ISO Abbreviation: SLAS Technol Subsets: MEDLINE

MeSH Terms: Machine Learning* , Nucleic Acids*/isolation & purification , Robotics*/methods , Robotics*/instrumentation , Gene Library* , Automation, Laboratory*/methods, Humans

Building a DNA barcode library of commercially exploited fish genetic resources (FGR) of the Gomti River, Ganga basin, implications for conservation and management.

Authors: Sahu A Singh M Sarkar UK

Source: Scientific reports [Sci Rep] 2025 Nov 28; Vol. 15 (1), pp. 42721. Date of Electronic Publication: 2025 Nov 28.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH Terms: DNA Barcoding, Taxonomic*/methods , Fishes*/genetics , Fishes*/classification , Conservation of Natural Resources* , Gene Library*, Animals ; Rivers ; Phylogeny ; India ; Biodiversity ; Electron Transport Complex IV/genetics

Simple and High-Throughput Fluorescence Assay Method for DNA Damage Analysis in Single-Stranded DNA-Encoded Library Synthesis.

Authors: Kim GA Kang S Win MN et al.

Source: Bioconjugate chemistry [Bioconjug Chem] 2025 Nov 19; Vol. 36 (11), pp. 2342-2347. Date of Electronic Publication: 2025 Jul 08.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 9010319 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4812 (Electronic) Linking ISSN: 10431802 NLM ISO Abbreviation: Bioconjug Chem Subsets: MEDLINE

MeSH Terms: DNA, Single-Stranded*/chemistry , DNA, Single-Stranded*/genetics , DNA Damage* , High-Throughput Screening Assays*/methods , Fluorescent Dyes*/chemistry , Gene Library*, Fluorescence ; Nucleic Acid Hybridization

Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery.

Authors: Wright SL Abdul-Aziz M Blaha GN et al.

Source: Molecular ecology resources [Mol Ecol Resour] 2025 Nov; Vol. 25 (8), pp. e70054. Date of Electronic Publication: 2025 Oct 06.

Publication Type: Journal Article

Journal Info: Publisher: Blackwell Country of Publication: England NLM ID: 101465604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-0998 (Electronic) Linking ISSN: 1755098X NLM ISO Abbreviation: Mol Ecol Resour Subsets: MEDLINE

MeSH Terms: DNA, Ancient*/isolation & purification , Microbiota*/genetics , Dental Calculus*/microbiology , Metagenomics*/methods , Mouth*/microbiology , DNA, Bacterial*/isolation & purification , DNA, Bacterial*/genetics , Gene Library* , Specimen Handling*/methods, Humans ; Archaeology/methods

Construction of a FOX-hunting library to systematically identify functional genes and the salt-tolerant line isolation in Brassica napus.

Authors: Chang W Chen L Xie X et al.

Source: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2025 Nov; Vol. 228, pp. 110255. Date of Electronic Publication: 2025 Jul 15.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE

MeSH Terms: Brassica napus*/genetics , Brassica napus*/metabolism , Gene Library* , Salt Tolerance*/genetics , Genes, Plant*, Plants, Genetically Modified ; Arabidopsis/genetics ; Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Plant Proteins/metabolism

Host Traits Impact the Outcome of Metagenomic Library Preparation From Dental Calculus Samples Across Diverse Mammals.

Authors: Moraitou M Richards JL Bolyos C et al.

Source: Molecular ecology resources [Mol Ecol Resour] 2025 Nov; Vol. 25 (8), pp. e70039. Date of Electronic Publication: 2025 Sep 01.

Publication Type: Journal Article

Journal Info: Publisher: Blackwell Country of Publication: England NLM ID: 101465604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-0998 (Electronic) Linking ISSN: 1755098X NLM ISO Abbreviation: Mol Ecol Resour Subsets: MEDLINE

MeSH Terms: Metagenomics*/methods , Dental Calculus*/microbiology , Mammals*/microbiology , Mammals*/classification , Microbiota* , Gene Library*, Animals ; Metagenome ; Computational Biology

BOLDistilled: Automated Construction of Comprehensive but Compact DNA Barcode Reference Libraries.

Authors: Prosser SWJ Floyd RM Thompson KA et al.

Source: Molecular ecology resources [Mol Ecol Resour] 2025 Nov; Vol. 25 (8), pp. e70043. Date of Electronic Publication: 2025 Sep 14.

Publication Type: Journal Article

Journal Info: Publisher: Blackwell Country of Publication: England NLM ID: 101465604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-0998 (Electronic) Linking ISSN: 1755098X NLM ISO Abbreviation: Mol Ecol Resour Subsets: MEDLINE

MeSH Terms: DNA Barcoding, Taxonomic*/methods , Gene Library* , Computational Biology*/methods, Electron Transport Complex IV/genetics ; Algorithms ; Sequence Analysis, DNA

A Rapid, High-Throughput Method for the Construction of Mutagenesis Libraries.

Authors: Lu Y Meng S Guan X et al.

Source: Biomolecules [Biomolecules] 2025 Oct 25; Vol. 15 (11). Date of Electronic Publication: 2025 Oct 25.

Publication Type: Journal Article

Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE

MeSH Terms: Gene Library* , Mutagenesis* , High-Throughput Screening Assays*/methods, DNA-Directed DNA Polymerase/metabolism ; Oligonucleotides/genetics ; Mutation

Thermal-bias PCR: generation of amplicon libraries without degenerate primer interference.

Authors: Moore SD; Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, United States.; Genomics and Bioinformatics Cluster, University of Central Florida, Orlando, Florida, United States.

Source: PeerJ [PeerJ] 2025 Oct 24; Vol. 13, pp. e20241. Date of Electronic Publication: 2025 Oct 24 (Print Publication: 2025).

Publication Type: Journal Article

Journal Info: Publisher: PeerJ Inc Country of Publication: United States NLM ID: 101603425 Publication Model: eCollection Cited Medium: Internet ISSN: 2167-8359 (Electronic) Linking ISSN: 21678359 NLM ISO Abbreviation: PeerJ Subsets: MEDLINE

MeSH Terms: DNA Primers*/genetics , Polymerase Chain Reaction*/methods , Real-Time Polymerase Chain Reaction*/methods , Gene Library*

Preparation of functional metagenomic libraries from low biomass samples using METa assembly and their application to capture antibiotic resistance genes.

Authors: Allman HM Bernate EP Franck E et al.

Source: MSystems [mSystems] 2025 Oct 22; Vol. 10 (10), pp. e0103925. Date of Electronic Publication: 2025 Sep 10.

Publication Type: Journal Article

Journal Info: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5077 (Electronic) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems Subsets: MEDLINE

MeSH Terms: Metagenomics*/methods , Gene Library* , Drug Resistance, Microbial*/genetics, Metagenome ; Biomass ; Bacteria/genetics ; Humans ; Microbiota/genetics

A DNA Part Library for Reliable Engineering of the Emerging Model Nematode Symbiotic Bacterium Xenorhabdus griffiniae HGB2511.

Authors: Larsson EM Wang OY Murray RM

Source: ACS synthetic biology [ACS Synth Biol] 2025 Oct 17; Vol. 14 (10), pp. 4122-4126. Date of Electronic Publication: 2025 Sep 25.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

MeSH Terms: Xenorhabdus*/genetics , Gene Library* , Genetic Engineering*/methods , Nematoda*/microbiology, Animals ; Symbiosis/genetics ; Promoter Regions, Genetic/genetics ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Operon

Development and Validation of an Automated DNA-Encoded Library Screening Data Analysis Platform: PB-DEL Autoscreening Analysis (PB-DELASA).

Authors: Dong K Meng X Diao H et al.

Source: Journal of chemical information and modeling [J Chem Inf Model] 2025 Oct 13; Vol. 65 (19), pp. 10487-10502. Date of Electronic Publication: 2025 Sep 15.

Publication Type: Journal Article; Validation Study

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101230060 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-960X (Electronic) Linking ISSN: 15499596 NLM ISO Abbreviation: J Chem Inf Model Subsets: MEDLINE

MeSH Terms: DNA*/chemistry , DNA*/genetics , Small Molecule Libraries*/chemistry , Small Molecule Libraries*/pharmacology , Gene Library*, Humans ; Automation ; Drug Discovery/methods ; High-Throughput Nucleotide Sequencing ; Drug Evaluation, Preclinical/methods

A comprehensive genetic catalog of human double-strand break repair.

Authors: de Alba EL Salguero I Giménez-Llorente D et al.

Source: Science (New York, N.Y.) [Science] 2025 Oct 02; Vol. 390 (6768), pp. eadr5048. Date of Electronic Publication: 2025 Oct 02.

Publication Type: Journal Article

Journal Info: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE

MeSH Terms: DNA Breaks, Double-Stranded* , DNA Repair*/genetics , Gene Library*, Humans ; Carcinoma, Renal Cell/genetics ; CRISPR-Associated Protein 9 ; CRISPR-Cas Systems ; DNA End-Joining Repair/genetics ; DNA-Binding Proteins/genetics ; Gene Editing ; Gene Knockout Techniques ; Genome, Human ; INDEL Mutation ; Kidney Neoplasms/genetics ; Transcription Factors/genetics ; Von Hippel-Lindau Tumor Suppressor Protein/genetics

Preparation of Illumina 16s Amplicon Sequencing Libraries with Peptide Nucleic Acids (PNAs) for the Analysis of Maize-Associated Microbiomes.

Authors: Wallace JG Griffis H

Source: Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2025 Oct 01; Vol. 2025 (10), pp. pdb.prot108583. Date of Electronic Publication: 2025 Oct 01.

Publication Type: Journal Article

Journal Info: Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 101524530 Publication Model: Electronic Cited Medium: Internet ISSN: 1559-6095 (Electronic) Linking ISSN: 15596095 NLM ISO Abbreviation: Cold Spring Harb Protoc Subsets: MEDLINE

MeSH Terms: Zea mays*/microbiology , RNA, Ribosomal, 16S*/genetics , Peptide Nucleic Acids*/genetics , Peptide Nucleic Acids*/metabolism , Microbiota* , Gene Library* , Sequence Analysis, DNA*/methods , Bacteria*/genetics , Bacteria*/classification, Polymerase Chain Reaction/methods ; DNA Primers/genetics ; DNA, Bacterial/genetics ; DNA, Bacterial/chemistry

Revealing genetic drivers of ovarian cancer and chemoresistance: insights from whole-genome CRISPR-knockout library screens.

Authors: Skipper TS Dickson KA Denes CE et al.

Source: Cellular oncology (Dordrecht, Netherlands) [Cell Oncol (Dordr)] 2025 Oct; Vol. 48 (5), pp. 1245-1265. Date of Electronic Publication: 2025 Aug 28.

Publication Type: Journal Article; Review

Journal Info: Publisher: Springer Country of Publication: Netherlands NLM ID: 101552938 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-3436 (Electronic) Linking ISSN: 22113428 NLM ISO Abbreviation: Cell Oncol (Dordr) Subsets: MEDLINE

MeSH Terms: Ovarian Neoplasms*/genetics , Ovarian Neoplasms*/drug therapy , Drug Resistance, Neoplasm*/genetics , Gene Knockout Techniques* , CRISPR-Cas Systems*/genetics , Gene Library* , Clustered Regularly Interspaced Short Palindromic Repeats*/genetics, Humans ; Female

Identifying rubber-related genes through developing a sense/antisense RNA expression mutant library of Taraxacum kok-saghyz Rodin.

Authors: Fan X Chen Q Hu L et al.

Source: Journal of integrative plant biology [J Integr Plant Biol] 2025 Oct; Vol. 67 (10), pp. 2658-2667. Date of Electronic Publication: 2025 Jul 11.

Publication Type: Journal Article

Journal Info: Publisher: Wiley-Blackwell Pub Country of Publication: China (Republic : 1949- ) NLM ID: 101250502 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1744-7909 (Electronic) Linking ISSN: 16729072 NLM ISO Abbreviation: J Integr Plant Biol Subsets: MEDLINE

MeSH Terms: Rubber*/metabolism , Taraxacum*/genetics , Taraxacum*/metabolism , Mutation*/genetics , Gene Library* , Genes, Plant*/genetics, Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plants, Genetically Modified

Genomic Characterization of Cryptosporidium spp. via iNextEra Library Preparation and Hybridization Bait Capture.

Authors: Bhuiyan MIU Agyabeng-Dadzie F Sullivan AH et al.

Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2978, pp. 41-64.

Publication Type: Journal Article

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH Terms: Cryptosporidium*/genetics , Gene Library* , Nucleic Acid Hybridization*/methods , Genomics*/methods , Genome, Protozoan*, High-Throughput Nucleotide Sequencing/methods ; DNA, Protozoan/genetics ; DNA, Protozoan/isolation & purification ; Feces/parasitology ; Cryptosporidiosis/parasitology ; Animals ; Humans ; Sequence Analysis, DNA/methods

High-Efficiency Transformation of Integrative Libraries Under Zeocin Selection in Pichia pastoris.

Authors: Boone M Eeckhaut H Callewaert N

Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2697, pp. 329-343.

Publication Type: Journal Article

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH Terms: Transformation, Genetic* , Gene Library* , Saccharomycetales*/genetics , Bleomycin*/pharmacology , Bleomycin*/analogs & derivatives, Plasmids/genetics

SCR Organism: Komagataella pastoris