Suchergebnisse - "GENE targeting"

Boosting Brain Clean-Up: Can Targeting UPS Genes Offer Neuroprotection?

Autoren: Mannan A Sharma A Singh TG

Quelle: Molecular neurobiology [Mol Neurobiol] 2025 Dec; Vol. 62 (12), pp. 16512-16556. Date of Electronic Publication: 2025 Aug 16.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 8900963 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-1182 (Electronic) Linking ISSN: 08937648 NLM ISO Abbreviation: Mol Neurobiol Subsets: MEDLINE

MeSH-Schlagworte: Neuroprotection*/genetics , Brain*/metabolism , Brain*/pathology , Ubiquitin*/metabolism , Ubiquitin*/genetics , Proteasome Endopeptidase Complex*/genetics , Proteasome Endopeptidase Complex*/metabolism , Gene Targeting*/methods, Humans ; Animals ; Neurodegenerative Diseases/genetics ; Neurodegenerative Diseases/metabolism

Multi-oncogene targeting in cancer therapy: A miRNA-driven pharmacological approach.

Autoren: Salunkhe SS Ghatage T Prabhakar BS

Quelle: Biochemical pharmacology [Biochem Pharmacol] 2025 Dec; Vol. 242 (Pt 4), pp. 117417. Date of Electronic Publication: 2025 Oct 10.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Elsevier Science Country of Publication: England NLM ID: 0101032 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2968 (Electronic) Linking ISSN: 00062952 NLM ISO Abbreviation: Biochem Pharmacol Subsets: MEDLINE

MeSH-Schlagworte: MicroRNAs*/genetics , MicroRNAs*/metabolism , MicroRNAs*/administration & dosage , Neoplasms*/genetics , Neoplasms*/drug therapy , Neoplasms*/metabolism , Oncogenes*/drug effects , Oncogenes*/genetics , Oncogenes*/physiology , Antineoplastic Agents*/administration & dosage , Antineoplastic Agents*/pharmacology , Antineoplastic Agents*/therapeutic use , Gene Targeting*/methods, Humans ; Animals ; Gene Expression Regulation, Neoplastic/drug effects

Comparing gene-targeting efficiency of Agrobacterium tumefaciens-mediated transformation and electroporation in the pathogenic fungus Trichosporon asahii JCM2466.

Autoren: Matsumoto Y Nakayama M Shimizu Y et al.

Quelle: Drug discoveries & therapeutics [Drug Discov Ther] 2025 Nov 15; Vol. 19 (5), pp. 337-345. Date of Electronic Publication: 2025 Oct 17.

Publikationsart: Journal Article; Comparative Study

Info zur Zeitschrift: Publisher: Shandong University China-Japan Cooperation Center for Drug Discovery & Screen Country of Publication: Japan NLM ID: 101493809 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1881-784X (Electronic) Linking ISSN: 18817831 NLM ISO Abbreviation: Drug Discov Ther Subsets: MEDLINE

MeSH-Schlagworte: Electroporation*/methods , Agrobacterium tumefaciens*/genetics , Trichosporon*/genetics , Gene Targeting*/methods , Transformation, Genetic*, Gene Transfer Techniques ; Ku Autoantigen/genetics ; Basidiomycota

SCR Organism: Trichosporon asahii

CRISPR/Cas9-mediated gene targeting at BBM2 locus demonstrates HDR-assisted precise knock-in in banana cv. Grand Naine.

Autoren: Singh S Chaudhary R Tiwari S

Quelle: Plant cell reports [Plant Cell Rep] 2025 Nov 06; Vol. 44 (12), pp. 265. Date of Electronic Publication: 2025 Nov 06.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE

MeSH-Schlagworte: Musa*/genetics , CRISPR-Cas Systems*/genetics , Gene Knock-In Techniques*/methods , Gene Targeting*/methods , Plant Proteins*/genetics , Plant Proteins*/metabolism , Recombinational DNA Repair*/genetics, Gene Editing/methods ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Plants, Genetically Modified

Precise knock-in of stress-responsive cis-regulatory elements using gene targeting for improving abiotic stress tolerance in plants.

Autoren: Ke Y Kong D Zhang W et al.

Quelle: The New phytologist [New Phytol] 2025 Sep; Vol. 247 (5), pp. 2147-2162. Date of Electronic Publication: 2025 Jul 12.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE

MeSH-Schlagworte: Stress, Physiological*/genetics , Arabidopsis*/genetics , Arabidopsis*/physiology , Gene Knock-In Techniques* , Gene Targeting* , Adaptation, Physiological*/genetics , Regulatory Sequences, Nucleic Acid*/genetics, Gene Expression Regulation, Plant ; Plants, Genetically Modified ; Promoter Regions, Genetic/genetics ; Plant Stomata/physiology ; Oxidative Stress ; Plant Leaves/physiology

Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting.

Autoren: Bhat PP Inamdar MS

Quelle: Stem cell research [Stem Cell Res] 2025 Sep; Vol. 87, pp. 103783. Date of Electronic Publication: 2025 Jul 25.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: England NLM ID: 101316957 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7753 (Electronic) Linking ISSN: 18735061 NLM ISO Abbreviation: Stem Cell Res Subsets: MEDLINE

MeSH-Schlagworte: CRISPR-Cas Systems*/genetics , Human Embryonic Stem Cells*/metabolism , Human Embryonic Stem Cells*/cytology , Actins*/genetics , Actins*/metabolism , Green Fluorescent Proteins*/metabolism , Green Fluorescent Proteins*/genetics , Genes, Reporter* , Gene Targeting*, Humans ; Cell Line ; Gene Editing

CRISPR/Cas9-mediated promoterless gene targeting reduces lysosome storage in MPS VII mice.

Autoren: Shan L He Q Zhang W et al.

Quelle: Science China. Life sciences [Sci China Life Sci] 2025 Sep; Vol. 68 (9), pp. 2697-2706. Date of Electronic Publication: 2025 Jul 02.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Science China Press, co-published with Springer Country of Publication: China NLM ID: 101529880 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1869-1889 (Electronic) Linking ISSN: 16747305 NLM ISO Abbreviation: Sci China Life Sci Subsets: MEDLINE

MeSH-Schlagworte: CRISPR-Cas Systems*/genetics , Mucopolysaccharidosis VII*/therapy , Mucopolysaccharidosis VII*/genetics , Mucopolysaccharidosis VII*/metabolism , Gene Targeting*/methods , Lysosomes*/metabolism, Animals ; Mice ; Glucuronidase/genetics ; Glucuronidase/metabolism ; Glucuronidase/blood ; Disease Models, Animal ; Genetic Vectors/genetics ; Promoter Regions, Genetic ; Genetic Therapy/methods ; Actins/genetics ; Adenoviridae/genetics ; Liver/metabolism

Generation of WT1-tdTomato knock-in cynomolgus monkey embryonic stem cell line, WT1-205 using CRISPR/CAS9-based gene targeting.

Autoren: Hyraht A Zhan F Guo R

Quelle: Stem cell research [Stem Cell Res] 2025 Sep; Vol. 87, pp. 103742. Date of Electronic Publication: 2025 May 24.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: England NLM ID: 101316957 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7753 (Electronic) Linking ISSN: 18735061 NLM ISO Abbreviation: Stem Cell Res Subsets: MEDLINE

MeSH-Schlagworte: CRISPR-Cas Systems*/genetics , Embryonic Stem Cells*/metabolism , Embryonic Stem Cells*/cytology , Gene Knock-In Techniques* , WT1 Proteins*/genetics , WT1 Proteins*/metabolism , Gene Targeting*, Animals ; Macaca fascicularis ; Cell Line ; Cell Differentiation ; Red Fluorescent Protein

Non-viral intron knock-ins for targeted gene integration into human T cells and for T-cell selection.

Autoren: Roth TL Lu J McClellan A et al.

Quelle: Nature biomedical engineering [Nat Biomed Eng] 2025 Aug; Vol. 9 (8), pp. 1309-1319. Date of Electronic Publication: 2025 Mar 07.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Springer Nature Country of Publication: England NLM ID: 101696896 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2157-846X (Electronic) Linking ISSN: 2157846X NLM ISO Abbreviation: Nat Biomed Eng Subsets: MEDLINE

MeSH-Schlagworte: Introns*/genetics , T-Lymphocytes*/metabolism , T-Lymphocytes*/cytology , Gene Knock-In Techniques*/methods , Gene Targeting*/methods, Humans ; Exons/genetics ; Receptors, Antigen, T-Cell/genetics ; Receptors, Chimeric Antigen/genetics

Precision oncolytic viral therapy in colorectal cancer: Genetic targeting and immune modulation for personalized treatment (Review).

Autoren: Sultan MH Zhan Q Wang Y et al.

Quelle: International journal of molecular medicine [Int J Mol Med] 2025 Jul; Vol. 56 (1). Date of Electronic Publication: 2025 May 09.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: D.A. Spandidos Country of Publication: Greece NLM ID: 9810955 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-244X (Electronic) Linking ISSN: 11073756 NLM ISO Abbreviation: Int J Mol Med Subsets: MEDLINE

MeSH-Schlagworte: Colorectal Neoplasms*/therapy , Colorectal Neoplasms*/immunology , Colorectal Neoplasms*/genetics , Oncolytic Virotherapy*/methods , Precision Medicine*/methods , Oncolytic Viruses*/genetics , Oncolytic Viruses*/immunology , Gene Targeting*/methods , Immunomodulation*, Humans ; Tumor Microenvironment/immunology ; Animals ; Immunotherapy/methods

Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion.

Autoren: Lu Y Wang J Xu Y et al.

Quelle: Trends in biotechnology [Trends Biotechnol] 2025 Jul; Vol. 43 (7), pp. 1743-1764. Date of Electronic Publication: 2025 Apr 07.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Science Publishers Country of Publication: England NLM ID: 8310903 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3096 (Electronic) Linking ISSN: 01677799 NLM ISO Abbreviation: Trends Biotechnol Subsets: MEDLINE

MeSH-Schlagworte: CRISPR-Cas Systems*/genetics , Gene Editing*/methods , Gene Targeting*/methods, Animals ; Mice ; Humans ; Genetic Therapy/methods ; Gene Knock-In Techniques/methods ; DNA Repair ; Hepatocytes/metabolism ; Deoxyribonuclease I/metabolism ; Deoxyribonuclease I/genetics

Homology-arm length of donor DNA affects the impact of Msh2 loss on homologous recombination-mediated gene targeting.

Autoren: Saito S Suzuki T Nohmi T et al.

Quelle: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2025 Jun 17; Vol. 122 (24), pp. e2508507122. Date of Electronic Publication: 2025 Jun 09.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE

MeSH-Schlagworte: MutS Homolog 2 Protein*/genetics , MutS Homolog 2 Protein*/metabolism , Homologous Recombination*/genetics , Gene Targeting*/methods , DNA*/genetics , Saccharomyces cerevisiae Proteins*/genetics , Saccharomyces cerevisiae Proteins*/metabolism, DNA Mismatch Repair/genetics ; Gene Editing/methods ; Humans ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism

Challenges of Studying Amelogenesis in Gene-Targeted Mouse Models.

Autoren: Smith CE Bartlett JD Simmer JP et al.

Quelle: International journal of molecular sciences [Int J Mol Sci] 2025 May 20; Vol. 26 (10). Date of Electronic Publication: 2025 May 20.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

MeSH-Schlagworte: Amelogenesis*/genetics , Gene Targeting*, Animals ; Mice ; Dental Enamel/metabolism

Protein-targeting reverse genetic approaches: the future of oocyte and preimplantation embryo research.

Autoren: Camlin NJ; Cell and Molecular Biology, School of Biological, Environmental and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, USA.

Quelle: Molecular human reproduction [Mol Hum Reprod] 2025 Apr 03; Vol. 31 (2).

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Oxford University Press for the European Society for Human Reproduction and Embryology Country of Publication: England NLM ID: 9513710 Publication Model: Print Cited Medium: Internet ISSN: 1460-2407 (Electronic) Linking ISSN: 13609947 NLM ISO Abbreviation: Mol Hum Reprod Subsets: MEDLINE

MeSH-Schlagworte: Oocytes*/metabolism , Blastocyst*/metabolism , Gene Targeting*/methods, Humans ; Animals ; Embryonic Development/genetics ; RNA Interference ; Female ; Indoleacetic Acids/pharmacology

Beyond a few bases: methods for large DNA insertion and gene targeting in plants.

Autoren: Vollen K Alonso JM Stepanova AN

Quelle: The Plant journal : for cell and molecular biology [Plant J] 2025 Mar; Vol. 121 (6), pp. e70099.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology Country of Publication: England NLM ID: 9207397 Publication Model: Print Cited Medium: Internet ISSN: 1365-313X (Electronic) Linking ISSN: 09607412 NLM ISO Abbreviation: Plant J Subsets: MEDLINE

MeSH-Schlagworte: Gene Editing*/methods , Gene Targeting*/methods , Mutagenesis, Insertional*/methods , Plants*/genetics , DNA, Plant*/genetics, CRISPR-Cas Systems/genetics ; Crops, Agricultural/genetics ; Genome, Plant/genetics ; Plants, Genetically Modified/genetics ; Plant Breeding/methods

Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome.

Autoren: Protacio RU Wahls WP

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2862, pp. 223-239.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Schizosaccharomyces*/genetics , Gene Targeting*/methods, Genome, Fungal ; DNA Mutational Analysis/methods ; Homologous Recombination ; Alleles ; Gene Editing/methods ; Genetic Loci

Gene Targeting via CRISPR/Cas9-Mediated DNA Double-Strand Break Induction in Rice.

Autoren: Nishizawa-Yokoi A Toki S

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2869, pp. 91-100.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Oryza*/genetics , Oryza*/metabolism , CRISPR-Cas Systems* , DNA Breaks, Double-Stranded* , Gene Targeting*/methods , Gene Editing*/methods, Plants, Genetically Modified/genetics ; Genome, Plant ; Homologous Recombination

Genetic targeting of myelinated primary afferent neurons using a new Nefh ^CreERT2 knock-in mouse.

Autoren: Chen JC Kaczmarczyk L de-Faria FM et al.

Quelle: Scientific reports [Sci Rep] 2025 Mar 29; Vol. 15 (1), pp. 10890. Date of Electronic Publication: 2025 Mar 29.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH-Schlagworte: Gene Knock-In Techniques* , Integrases*/genetics , Integrases*/metabolism , Neurofilament Proteins*/genetics , Neurofilament Proteins*/metabolism , Gene Targeting*/methods , Neurons, Afferent*/metabolism , Nerve Fibers, Myelinated*/metabolism, Animals ; Mice ; Mice, Transgenic ; Tamoxifen/pharmacology

Improvement of Targeting Efficiency by Promoter Replacement of Markers in Integration Vectors.

Autoren: Matsuyama A Hashimoto A Arioka M et al.

Quelle: Genes to cells : devoted to molecular & cellular mechanisms [Genes Cells] 2025 Mar; Vol. 30 (2), pp. e70013.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Blackwell Science Ltd Country of Publication: England NLM ID: 9607379 Publication Model: Print Cited Medium: Internet ISSN: 1365-2443 (Electronic) Linking ISSN: 13569597 NLM ISO Abbreviation: Genes Cells Subsets: MEDLINE

MeSH-Schlagworte: Promoter Regions, Genetic* , Genetic Vectors*/genetics , Schizosaccharomyces*/genetics , Gene Targeting*/methods, Recombination, Genetic ; Genetic Markers

In vivo expansion of gene-targeted hepatocytes through transient inhibition of an essential gene.

Autoren: De Giorgi M Park SH Castoreno A et al.

Quelle: Science translational medicine [Sci Transl Med] 2025 Feb 12; Vol. 17 (785), pp. eadk3920. Date of Electronic Publication: 2025 Feb 12.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101505086 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1946-6242 (Electronic) Linking ISSN: 19466234 NLM ISO Abbreviation: Sci Transl Med Subsets: MEDLINE

MeSH-Schlagworte: Hepatocytes*/metabolism , Hepatocytes*/cytology , Genes, Essential*/genetics , Gene Targeting*/methods, Animals ; Humans ; Mice ; Gene Editing/methods ; Hydrolases/genetics ; Hydrolases/metabolism ; Transgenes/genetics ; Liver/metabolism ; DNA Repair ; Mice, Inbred C57BL