Search Results - "Enzyme-Linked Immunosorbent Assay"

Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins.

Authors: Watcharavongtip P Jermsutjarit P Tantituvanont A et al.

Source: The veterinary quarterly [Vet Q] 2025 Dec; Vol. 45 (1), pp. 1-11. Date of Electronic Publication: 2025 Jan 08.

Publication Type: Journal Article

Journal Info: Publisher: Taylor & Francis Country of Publication: England NLM ID: 7909485 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1875-5941 (Electronic) Linking ISSN: 01652176 NLM ISO Abbreviation: Vet Q Subsets: MEDLINE

MeSH Terms: Enzyme-Linked Immunosorbent Assay*/veterinary , Enzyme-Linked Immunosorbent Assay*/methods , Picornaviridae*/immunology , Antibodies, Viral*/blood , Antibodies, Viral*/immunology , Swine Diseases*/virology , Swine Diseases*/diagnosis , Swine Diseases*/immunology , Sensitivity and Specificity* , Viral Nonstructural Proteins*/immunology , Viral Nonstructural Proteins*/genetics , Escherichia coli*/genetics, Animals ; Swine ; Baculoviridae/genetics ; Picornaviridae Infections/veterinary ; Picornaviridae Infections/diagnosis ; Picornaviridae Infections/immunology ; Recombinant Proteins/immunology ; Viral Vaccines/immunology ; Vaccination/veterinary ; Diagnosis, Differential

SCR Organism: Senecavirus A

Expression of BVDV E2 protein in CHO-S cells and development of an indirect ELISA for serological detection.

Authors: Wang J Zhang A Hou Z et al.

Source: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2025 Jul 24; Vol. 15, pp. 1631027. Date of Electronic Publication: 2025 Jul 24 (Print Publication: 2025).

Publication Type: Journal Article; Validation Study

Journal Info: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE

MeSH Terms: Viral Envelope Proteins*/blood , Viral Envelope Proteins*/isolation & purification , Enzyme-Linked Immunosorbent Assay*/methods, CHO Cells ; Cricetulus ; Animals ; Sensitivity and Specificity ; Cattle

Strictly screening of HTLV-1/2 peptides can drive the development of rapid point-of-care tests.

Authors: Cox LJ Fraga VG Fujiwara RT et al.

Source: Journal of virological methods [J Virol Methods] 2025 Jul; Vol. 336, pp. 115170. Date of Electronic Publication: 2025 Apr 28.

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE

MeSH Terms: Human T-lymphotropic virus 1*/isolation & purification , Human T-lymphotropic virus 2*/isolation & purification , Point-of-Care Testing* , Enzyme-Linked Immunosorbent Assay*/methods, Epitopes, B-Lymphocyte/analysis ; Viral Proteins/analysis ; Viral Proteins/genetics ; HTLV-I Infections/diagnosis ; HTLV-I Infections/virology ; HTLV-II Infections/diagnosis ; HTLV-II Infections/virology ; Humans

Chlamydia trachomatis pgp3 ELISA.

Authors: da Silva FC Kamuyu G Yokoya K et al.

Source: Journal of immunological methods [J Immunol Methods] 2025 Jun; Vol. 541, pp. 113879. Date of Electronic Publication: 2025 May 13.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1305440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7905 (Electronic) Linking ISSN: 00221759 NLM ISO Abbreviation: J Immunol Methods Subsets: MEDLINE

MeSH Terms: Chlamydia trachomatis*/immunology , Chlamydia Infections*/diagnosis , Chlamydia Infections*/immunology , Chlamydia Infections*/microbiology , Enzyme-Linked Immunosorbent Assay*/methods , Antigens, Bacterial*/immunology , Antigens, Bacterial*/isolation & purification , Bacterial Proteins*/immunology , Bacterial Proteins*/isolation & purification, Humans ; Male ; Female ; Infant ; Child, Preschool ; Child ; Adolescent ; Antibodies, Bacterial/immunology

Clinical Value of Different Test Methods in Diagnosing Mycoplasma pneumoniae Infection in Children.

Authors: Sun X Yao M Xu P et al.

Source: Clinical pediatrics [Clin Pediatr (Phila)] 2025 Jan; Vol. 64 (1), pp. 36-41. Date of Electronic Publication: 2024 Apr 12.

Publication Type: Journal Article

Journal Info: Publisher: Sage Publications Country of Publication: United States NLM ID: 0372606 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1938-2707 (Electronic) Linking ISSN: 00099228 NLM ISO Abbreviation: Clin Pediatr (Phila) Subsets: MEDLINE

MeSH Terms: Pneumonia, Mycoplasma*/diagnosis , Mycoplasma pneumoniae*/isolation & purification , Mycoplasma pneumoniae*/genetics , Enzyme-Linked Immunosorbent Assay*/methods , Polymerase Chain Reaction*/methods, Humans ; Female ; Male ; Child, Preschool ; Child ; Sensitivity and Specificity ; Agglutination Tests/methods

Development and evaluation of egg yolk-derived antibodies for ELISA diagnostics of African Animal Trypanosomiasis.

Authors: Ousmane T Hamidou I Christian TM et al.

Source: Veterinary parasitology [Vet Parasitol] 2025 Jan; Vol. 333, pp. 110354. Date of Electronic Publication: 2024 Nov 17.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7602745 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2550 (Electronic) Linking ISSN: 03044017 NLM ISO Abbreviation: Vet Parasitol Subsets: MEDLINE

MeSH Terms: Enzyme-Linked Immunosorbent Assay*/veterinary , Enzyme-Linked Immunosorbent Assay*/methods , Enzyme-Linked Immunosorbent Assay*/standards , Chickens* , Egg Yolk*/immunology , Egg Yolk*/chemistry , Antibodies, Protozoan*/blood , Trypanosomiasis, African*/diagnosis , Trypanosomiasis, African*/veterinary , Trypanosomiasis, African*/immunology, Animals ; Cattle ; Trypanosoma brucei brucei/immunology ; Immunoglobulins/immunology ; Immunoglobulin G/blood ; Cattle Diseases/diagnosis ; Cattle Diseases/parasitology

Unlocking the potential of dried blood spot sampling for SARS-CoV-2 antibody detection: A pathway to efficient pandemic surveillance.

Authors: Alharbi AM; Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi Arabia. Electronic address: a.alharbii@tu.edu.sa.

Source: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2025 Jan; Vol. 111 (1), pp. 116581. Date of Electronic Publication: 2024 Nov 04.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE

MeSH Terms: Antibodies, Viral*/blood , COVID-19*/diagnosis , COVID-19*/epidemiology , Dried Blood Spot Testing*/methods , SARS-CoV-2*/immunology , Immunoglobulin G*/blood , Enzyme-Linked Immunosorbent Assay*/methods, Humans ; Female ; Male ; Middle Aged ; Adult ; COVID-19 Serological Testing/methods ; Seroepidemiologic Studies ; Young Adult ; Aged ; Pandemics

Comparative assessment of brucellosis detection in dogs: In-house ELISA versus Rose Bengal Plate Test utilizing rough and smooth antigens.

Authors: Akar K Yücetepe AG Ekin İH et al.

Source: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2025 Jan; Vol. 116, pp. 102277. Date of Electronic Publication: 2024 Nov 17.

Publication Type: Journal Article; Comparative Study

Journal Info: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 7808924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1667 (Electronic) Linking ISSN: 01479571 NLM ISO Abbreviation: Comp Immunol Microbiol Infect Dis Subsets: MEDLINE

MeSH Terms: Brucellosis*/veterinary , Brucellosis*/epidemiology , Brucellosis*/diagnosis , Enzyme-Linked Immunosorbent Assay*/veterinary , Enzyme-Linked Immunosorbent Assay*/methods , Dog Diseases*/microbiology , Dog Diseases*/diagnosis , Dog Diseases*/epidemiology , Dog Diseases*/immunology , Rose Bengal* , Antibodies, Bacterial*/blood , Brucella canis*/immunology , Antigens, Bacterial*/immunology , Antigens, Bacterial*/blood, Animals ; Dogs ; Seroepidemiologic Studies ; Sensitivity and Specificity ; Brucella/immunology ; Lipopolysaccharides/immunology ; Brucella abortus/immunology ; Female ; Male

Development of a simplified model for assessing bovine leukemia virus transmission risk using enzyme-linked immunosorbent assay sample/positive ratios.

Authors: Hamada Y Hiroe T Oomoto T et al.

Source: Research in veterinary science [Res Vet Sci] 2025 Dec; Vol. 197, pp. 105972. Date of Electronic Publication: 2025 Nov 08.

Publication Type: Journal Article

Journal Info: Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE

MeSH Terms: Enzootic Bovine Leukosis*/transmission , Enzootic Bovine Leukosis*/virology , Enzootic Bovine Leukosis*/diagnosis , Leukemia Virus, Bovine*/isolation & purification , Leukemia Virus, Bovine*/physiology, Animals ; Cattle ; Enzyme-Linked Immunosorbent Assay/veterinary ; Enzyme-Linked Immunosorbent Assay/methods ; Real-Time Polymerase Chain Reaction/veterinary ; Viral Load/veterinary ; Female ; Japan/epidemiology ; Sensitivity and Specificity

A comparative study between milk- and serum-based antibody detection assays for Johne's disease in New Zealand dairy cattle.

Authors: Venkatesh KM Lopez-Villalobos N Gupta SK et al.

Source: Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2025 Dec; Vol. 155, pp. 102679. Date of Electronic Publication: 2025 Aug 27.

Publication Type: Journal Article; Comparative Study

Journal Info: Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE

MeSH Terms: Paratuberculosis*/diagnosis , Paratuberculosis*/microbiology , Paratuberculosis*/blood , Paratuberculosis*/immunology , Mycobacterium avium subsp. paratuberculosis*/immunology , Mycobacterium avium subsp. paratuberculosis*/genetics , Mycobacterium avium subsp. paratuberculosis*/isolation & purification , Milk*/microbiology , Milk*/immunology , Cattle Diseases*/diagnosis , Cattle Diseases*/microbiology , Cattle Diseases*/blood , Cattle Diseases*/immunology , Antibodies, Bacterial*/blood , Antibodies, Bacterial*/analysis, Animals ; Cattle ; New Zealand/epidemiology ; Enzyme-Linked Immunosorbent Assay/veterinary ; Enzyme-Linked Immunosorbent Assay/methods ; Feces/microbiology ; Predictive Value of Tests ; Reproducibility of Results ; Polymerase Chain Reaction/veterinary ; Dairying ; Female ; Reagent Kits, Diagnostic

Calcitonin gene-related peptide and headache: Comparison of two commonly used assay kits highlights the perils of measuring neuropeptides with enzyme-linked immunosorbent assays.

Authors: Garelja ML Rees TA Hay DL

Source: Headache [Headache] 2025 Nov-Dec; Vol. 65 (10), pp. 1744-1753. Date of Electronic Publication: 2025 Aug 07.

Publication Type: Journal Article; Comparative Study

Journal Info: Publisher: Wiley Country of Publication: United States NLM ID: 2985091R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4610 (Electronic) Linking ISSN: 00178748 NLM ISO Abbreviation: Headache Subsets: MEDLINE

MeSH Terms: Calcitonin Gene-Related Peptide*/analysis , Calcitonin Gene-Related Peptide*/blood , Reagent Kits, Diagnostic*/standards , Headache*/diagnosis , Headache*/blood , Headache*/metabolism, Enzyme-Linked Immunosorbent Assay/methods ; Enzyme-Linked Immunosorbent Assay/standards ; Humans ; Animals ; Mice ; Biomarkers/analysis ; Biomarkers/blood ; Protein Isoforms ; Reproducibility of Results ; Migraine Disorders/diagnosis

A sandwich ELISA for the quantification of the anticancer peptide CIGB-552 in human plasma.

Authors: Gómez Hernández NA Pérez GL Arteaga AV et al.

Source: Analytical biochemistry [Anal Biochem] 2025 Mar; Vol. 698, pp. 115725. Date of Electronic Publication: 2024 Nov 27.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE

MeSH Terms: Enzyme-Linked Immunosorbent Assay*/methods , Antineoplastic Agents*/blood , Antineoplastic Agents*/pharmacokinetics, Humans ; Peptides/blood ; Limit of Detection

Heterologous expression, purification and generation of specific antibodies for Annexin A8.

Authors: Lin Z Sun W Zhao X et al.

Source: Protein expression and purification [Protein Expr Purif] 2025 Mar; Vol. 227, pp. 106644. Date of Electronic Publication: 2024 Dec 12.

Publication Type: Journal Article

Journal Info: Publisher: Academic Press Country of Publication: United States NLM ID: 9101496 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0279 (Electronic) Linking ISSN: 10465928 NLM ISO Abbreviation: Protein Expr Purif Subsets: MEDLINE

MeSH Terms: Recombinant Proteins*/genetics , Recombinant Proteins*/immunology , Recombinant Proteins*/biosynthesis , Recombinant Proteins*/isolation & purification , Recombinant Proteins*/chemistry , Escherichia coli*/genetics , Escherichia coli*/metabolism , Enzyme-Linked Immunosorbent Assay* , Annexins*/genetics , Annexins*/immunology , Annexins*/chemistry , Annexins*/isolation & purification, Humans ; Animals ; Mice ; Antibodies, Monoclonal/biosynthesis ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/genetics ; Antibodies, Monoclonal/isolation & purification ; Annexin A2/genetics ; Annexin A2/immunology ; Annexin A2/biosynthesis ; Annexin A2/isolation & purification ; Gene Expression ; Mice, Inbred BALB C ; Antibody Specificity ; Saccharomycetales

SCR Organism: Komagataella pastoris

Rational hapten design, antibody preparation, and immunoassay development for rapid screening xylazine in biological samples.

Authors: Luo L Zhang Y Zhao X et al.

Source: Food chemistry [Food Chem] 2025 Feb 15; Vol. 465 (Pt 2), pp. 142054. Date of Electronic Publication: 2024 Nov 17.

Publication Type: Journal Article; Evaluation Study

Journal Info: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE

MeSH Terms: Haptens*/chemistry , Haptens*/immunology , Antibodies, Monoclonal*/immunology , Antibodies, Monoclonal*/chemistry , Enzyme-Linked Immunosorbent Assay* , Xylazine*/analysis, Humans ; Animals ; Food Contamination/analysis ; Immunoassay/methods ; Limit of Detection ; Mice

Development of a nanobody-horseradish peroxidase fusion-based competitive ELISA to rapidly and sensitively detect Enrofloxacin residues in animal-derived foods.

Authors: Yang M Xu Q Gu K et al.

Source: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2025 Feb 15; Vol. 327, pp. 125309. Date of Electronic Publication: 2024 Oct 21.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: England NLM ID: 9602533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3557 (Electronic) Linking ISSN: 13861425 NLM ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Subsets: MEDLINE

MeSH Terms: Enzyme-Linked Immunosorbent Assay*/methods , Horseradish Peroxidase*/chemistry , Horseradish Peroxidase*/metabolism , Enrofloxacin*/analysis , Single-Domain Antibodies*/immunology , Limit of Detection* , Milk*/chemistry, Animals ; Camelus ; Food Contamination/analysis ; Recombinant Fusion Proteins ; Food Analysis/methods ; Drug Residues/analysis

Exploring potential application of anti-dengue NS1 human polyclonal antibodies for detection of dengue virus infection.

Authors: Abraham PR Veeraiyan E Dhotre A et al.

Source: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2025 Feb; Vol. 111 (2), pp. 116588. Date of Electronic Publication: 2024 Nov 08.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE

MeSH Terms: Dengue*/diagnosis , Dengue*/immunology , Viral Nonstructural Proteins*/immunology , Antibodies, Viral*/blood , Antibodies, Viral*/immunology , Enzyme-Linked Immunosorbent Assay*/methods , Dengue Virus*/immunology , Sensitivity and Specificity*, Humans ; Antigens, Viral/immunology ; Immunoglobulin G/blood ; Immunoglobulin G/immunology

Detection of Salmonella by competitive ELISA of lipopolysaccharide secreted into the culture medium.

Authors: Kiseleva E Mikhailopulo K Sviridov O

Source: Analytical biochemistry [Anal Biochem] 2025 Feb; Vol. 697, pp. 115695. Date of Electronic Publication: 2024 Oct 23.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE

MeSH Terms: Enzyme-Linked Immunosorbent Assay*/methods , Lipopolysaccharides*/analysis , Salmonella*/isolation & purification , Salmonella*/immunology , Culture Media*/chemistry, Serum Albumin, Bovine/chemistry ; Antibodies, Monoclonal/immunology ; Food Microbiology ; Humans ; Animals

Nanopore-assisted ELISA for ultrasensitive, portable, and on-site detection of ricin.

Authors: Chen J Su Z Li W et al.

Source: Talanta [Talanta] 2025 Feb 01; Vol. 283, pp. 127136. Date of Electronic Publication: 2024 Oct 31.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE

MeSH Terms: Ricin*/analysis , Ricin*/immunology , Enzyme-Linked Immunosorbent Assay*/methods , Nanopores*, Limit of Detection ; Food Contamination/analysis ; DNA Probes/chemistry

Development of Polyclonal Antibodies-Based Serological Method for the Detection of Calanthe Mild Mosaic Virus and Application in Virus Certification Programme.

Authors: Srivastava N Kumar R Kapoor R et al.

Source: Molecular biotechnology [Mol Biotechnol] 2025 Feb; Vol. 67 (2), pp. 628-637. Date of Electronic Publication: 2024 Feb 16.

Publication Type: Journal Article

Journal Info: Publisher: Springer Country of Publication: Switzerland NLM ID: 9423533 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0305 (Electronic) Linking ISSN: 10736085 NLM ISO Abbreviation: Mol Biotechnol Subsets: MEDLINE

MeSH Terms: Potyvirus*/genetics , Potyvirus*/immunology , Potyvirus*/isolation & purification , Antibodies, Viral*/immunology , Antibodies, Viral*/blood , Orchidaceae*/virology , Plant Diseases*/virology , Enzyme-Linked Immunosorbent Assay*/methods , Capsid Proteins*/genetics , Capsid Proteins*/immunology, Animals ; Rabbits ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/immunology

Generation and validation of antibody 42B1 recognizing galactose-deficient IgG for diagnosis of chronic inflammatory diseases.

Authors: Morishima N Iwaisako M Kamada Y et al.

Source: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2025 Jan 30; Vol. 566, pp. 120052. Date of Electronic Publication: 2024 Nov 16.

Publication Type: Journal Article; Validation Study

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1302422 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3492 (Electronic) Linking ISSN: 00098981 NLM ISO Abbreviation: Clin Chim Acta Subsets: MEDLINE

MeSH Terms: Immunoglobulin G*/blood , Enzyme-Linked Immunosorbent Assay*, Humans ; Chronic Disease ; Male ; Female ; Middle Aged ; Galactose ; Adult ; Inflammatory Bowel Diseases/blood ; Inflammatory Bowel Diseases/diagnosis ; Inflammatory Bowel Diseases/immunology ; Inflammation/blood ; Inflammation/diagnosis ; Liver Diseases/diagnosis ; Liver Diseases/blood ; Liver Diseases/immunology ; Aged