Search Results - "Endodeoxyribonucleases"

Deciphering letermovir's mode of action and resistance mutation effects.

Authors: Gourin C Flores T Lefèvre C et al.

Source: Antiviral research [Antiviral Res] 2025 Dec; Vol. 244, pp. 106289. Date of Electronic Publication: 2025 Oct 09.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8109699 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9096 (Electronic) Linking ISSN: 01663542 NLM ISO Abbreviation: Antiviral Res Subsets: MEDLINE

MeSH Terms: Drug Resistance, Viral*/genetics , Antiviral Agents*/pharmacology , Cytomegalovirus*/drug effects , Cytomegalovirus*/genetics , Acetates*/pharmacology , Quinazolines*/pharmacology , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/chemistry, Humans ; HEK293 Cells ; Mutation ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Protein Binding ; Viral Structural Proteins

Multiple CRISPR zones-driven ultrasensitive detection of DNA via CRISPR-Cas12a and ligation-rolling circle amplification.

Authors: Lin G Li J Zhang K

Source: Talanta [Talanta] 2025 Dec 01; Vol. 295, pp. 128336. Date of Electronic Publication: 2025 May 19.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE

MeSH Terms: CRISPR-Cas Systems*/genetics , DNA*/analysis , DNA*/genetics , Nucleic Acid Amplification Techniques*/methods , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/genetics , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/metabolism, Limit of Detection ; Humans ; Polymorphism, Single Nucleotide ; Bacterial Proteins

AND logic gate-based alternating PER-Cas12a signal amplification system for ultrasensitive detection of sEVs.

Authors: Wang M Zhang X Fan J et al.

Source: Talanta [Talanta] 2025 Dec 01; Vol. 295, pp. 128411. Date of Electronic Publication: 2025 May 27.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE

MeSH Terms: Breast Neoplasms*/diagnosis , Breast Neoplasms*/pathology , Extracellular Vesicles*/metabolism , Extracellular Vesicles*/chemistry , Biomarkers, Tumor* , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/genetics , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics , Nucleic Acid Amplification Techniques*/methods , Logic*, Humans ; Female ; Mucin-1/metabolism ; Mucin-1/genetics ; Limit of Detection ; Epithelial Cell Adhesion Molecule/metabolism ; Biosensing Techniques/methods ; CRISPR-Cas Systems ; Bacterial Proteins

A novel fluorescent sensing platform for miRNA-210 detection based on signal amplification via SDA and self-assembly Cas12a system via RCT.

Authors: Shen C Chen X Yu Y et al.

Source: Talanta [Talanta] 2025 Dec 01; Vol. 295, pp. 128382. Date of Electronic Publication: 2025 May 24.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE

MeSH Terms: MicroRNAs*/analysis , MicroRNAs*/genetics , Biosensing Techniques*/methods , Nucleic Acid Amplification Techniques*/methods , CRISPR-Cas Systems* , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/genetics , CRISPR-Associated Proteins*/chemistry , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics , Fluorescent Dyes*/chemistry, Humans ; Limit of Detection ; Spectrometry, Fluorescence ; Fluorescence ; Bacterial Proteins

A buffer-tuning strategy to profile domain-specific activity of chimeric I-TevI/CRISPR gene editors in vitro.

Authors: Loedige KW White AL McMurrough TA et al.

Source: Scientific reports [Sci Rep] 2025 Nov 28; Vol. 15 (1), pp. 42742. Date of Electronic Publication: 2025 Nov 28.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH Terms: Gene Editing*/methods , CRISPR-Cas Systems* , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics, Buffers ; RNA, Guide, CRISPR-Cas Systems/genetics ; Protein Domains ; DNA Cleavage ; Humans ; DNA/metabolism

Programmable no-nonspecific genetic analytical system via dual-circle-based rolling circle amplification with an efficient CRISPR/Cas12a biosensing strategy.

Authors: Feng C Wang Y Liu C et al.

Source: Scientific reports [Sci Rep] 2025 Nov 19; Vol. 15 (1), pp. 40723. Date of Electronic Publication: 2025 Nov 19.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH Terms: CRISPR-Cas Systems*/genetics , Nucleic Acid Amplification Techniques*/methods , Biosensing Techniques*/methods , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics, Humans ; DNA, Single-Stranded/genetics ; DNA, Circular/genetics ; Limit of Detection ; Bacterial Proteins ; CRISPR-Associated Proteins

TdT/Cas12a cascade amplification biosensor for sensitive ALP activity detection.

Authors: Pei C Yan B Wang Y et al.

Source: The Analyst [Analyst] 2025 Nov 17; Vol. 150 (23), pp. 5330-5337. Date of Electronic Publication: 2025 Nov 17.

Publication Type: Journal Article

Journal Info: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE

MeSH Terms: Biosensing Techniques*/methods , Alkaline Phosphatase*/metabolism , Alkaline Phosphatase*/analysis , DNA Nucleotidylexotransferase*/metabolism , DNA Nucleotidylexotransferase*/chemistry , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics , Bacterial Proteins*/metabolism , Bacterial Proteins*/genetics , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/genetics, Humans ; HeLa Cells ; CRISPR-Cas Systems ; Limit of Detection

CRISPR/Cas12a-SERS biosensor based on sea urchin-like AuNPs for the detection of β-thalassemia mutant gene CD31.

Authors: Xu H Chen H Li Y et al.

Source: Mikrochimica acta [Mikrochim Acta] 2025 Nov 14; Vol. 192 (12), pp. 810. Date of Electronic Publication: 2025 Nov 14.

Publication Type: Journal Article

Journal Info: Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE

MeSH Terms: Biosensing Techniques*/methods , beta-Thalassemia*/genetics , beta-Thalassemia*/diagnosis , CRISPR-Cas Systems* , Spectrum Analysis, Raman*/methods , Gold*/chemistry , Metal Nanoparticles*/chemistry , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/metabolism , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/genetics, Animals ; Mutation ; Sea Urchins/chemistry ; Limit of Detection ; Humans ; DNA, Single-Stranded/chemistry ; DNA, Single-Stranded/genetics ; Sulfhydryl Compounds/chemistry ; Bacterial Proteins ; Benzoates

Cas12a-assisted split crRNA complex for analysis and detection of diverse entities.

Authors: Xiao Y Zhao R Bao Y et al.

Source: Nucleic acids research [Nucleic Acids Res] 2025 Nov 13; Vol. 53 (21).

Publication Type: Journal Article

Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

MeSH Terms: CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/genetics , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics , CRISPR-Cas Systems* , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism , Biosensing Techniques*/methods, MicroRNAs/genetics ; MicroRNAs/analysis ; DNA, Single-Stranded/genetics ; DNA/genetics

Stable Converter-Powered Cascade Amplification of DNAzyme and Cas12a for Electrochemiluminescent Detection of DNA Adenine Methyltransferase.

Authors: Wang Z Zhao ML Yan L et al.

Source: Analytical chemistry [Anal Chem] 2025 Nov 04; Vol. 97 (43), pp. 24209-24216. Date of Electronic Publication: 2025 Oct 21.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

MeSH Terms: DNA, Catalytic*/metabolism , DNA, Catalytic*/chemistry , Electrochemical Techniques*/methods , Site-Specific DNA-Methyltransferase (Adenine-Specific)*/analysis , Site-Specific DNA-Methyltransferase (Adenine-Specific)*/metabolism , Biosensing Techniques*/methods , Luminescent Measurements*/methods , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/chemistry , Bacterial Proteins*/metabolism , Bacterial Proteins*/chemistry , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/chemistry

Allosteric Activation of Cas12a via PAM Topological Engineering for Direct and Rapid Detection of Nucleases.

Authors: Tao XL Lei YM Zhou XM et al.

Source: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2025 Nov 03; Vol. 64 (45), pp. e202515521. Date of Electronic Publication: 2025 Sep 16.

Publication Type: Journal Article

Journal Info: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 0370543 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-3773 (Electronic) Linking ISSN: 14337851 NLM ISO Abbreviation: Angew Chem Int Ed Engl Subsets: MEDLINE

MeSH Terms: CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/chemistry , DNA*/chemistry , DNA*/metabolism , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/chemistry, Allosteric Regulation ; CRISPR-Cas Systems ; Bacterial Proteins

A temperature-sensitive CRISPR-Cas12a system for sterile insect technique.

Authors: Nguyen C Omotayo AI Sanz Juste S et al.

Source: Nature communications [Nat Commun] 2025 Nov 03; Vol. 16 (1), pp. 9677. Date of Electronic Publication: 2025 Nov 03.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

MeSH Terms: CRISPR-Cas Systems*/genetics , Infertility, Male*/genetics , CRISPR-Associated Proteins*/genetics , CRISPR-Associated Proteins*/metabolism , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/metabolism , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism, Animals ; Male ; Female ; Temperature ; RNA, Guide, CRISPR-Cas Systems/genetics ; Animals, Genetically Modified ; Gene Editing/methods

A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi.

Authors: Hillebrand GH Carlin SC Giacobe EJ et al.

Source: Molecular microbiology [Mol Microbiol] 2025 Nov; Vol. 124 (5), pp. 449-461. Date of Electronic Publication: 2025 Sep 13.

Publication Type: Journal Article

Journal Info: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2958 (Electronic) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE

MeSH Terms: Streptococcus agalactiae*/genetics , Gene Editing*/methods , CRISPR-Cas Systems*/genetics , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism , CRISPR-Associated Proteins*/genetics , CRISPR-Associated Proteins*/metabolism , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/metabolism, Plasmids/genetics ; Genome, Bacterial ; Mutagenesis ; Enterococcus faecium/genetics

Phage-encoded TelN inhibits bacterial Mre11-Rad50 nuclease to protect hairpin telomeres.

Authors: Houmel M Pellaton N Anchimiuk A et al.

Source: The EMBO journal [EMBO J] 2025 Nov; Vol. 44 (22), pp. 6649-6674. Date of Electronic Publication: 2025 Oct 15.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE

MeSH Terms: Escherichia coli*/genetics , Escherichia coli*/virology , Escherichia coli*/metabolism , Telomere*/metabolism , Telomere*/genetics , Escherichia coli Proteins*/metabolism , Escherichia coli Proteins*/genetics , Escherichia coli Proteins*/antagonists & inhibitors , Viral Proteins*/metabolism , Viral Proteins*/genetics , Bacteriophages*/genetics , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics , Exodeoxyribonucleases*/metabolism , Exodeoxyribonucleases*/genetics

Multiscale identification of DNASE1L3 as a key target in MASLD progression to hepatocellular carcinoma.

Authors: Xia L Yan X Zhang H

Source: Pathology, research and practice [Pathol Res Pract] 2025 Nov; Vol. 275, pp. 156192. Date of Electronic Publication: 2025 Aug 31.

Publication Type: Journal Article

Journal Info: Publisher: Gustav Fischer Verlag Country of Publication: Germany NLM ID: 7806109 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0631 (Electronic) Linking ISSN: 03440338 NLM ISO Abbreviation: Pathol Res Pract Subsets: MEDLINE

MeSH Terms: Carcinoma, Hepatocellular*/pathology , Carcinoma, Hepatocellular*/genetics , Carcinoma, Hepatocellular*/metabolism , Liver Neoplasms*/pathology , Liver Neoplasms*/genetics , Liver Neoplasms*/metabolism , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/metabolism , Fatty Liver*/pathology , Fatty Liver*/genetics, Humans ; Disease Progression ; Cell Proliferation/genetics ; Gene Expression Regulation, Neoplastic ; Cell Line, Tumor

Photocontrolled dissociation and toehold-mediated strand displacement-based synergistic regulation of CRISPR-Cas12a.

Authors: Wu JN Li C Liu Z et al.

Source: Nucleic acids research [Nucleic Acids Res] 2025 Oct 28; Vol. 53 (20).

Publication Type: Journal Article

Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

MeSH Terms: CRISPR-Cas Systems*/radiation effects , CRISPR-Cas Systems*/genetics , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/genetics , Gene Editing*/methods , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics , Bacterial Proteins*/metabolism , Bacterial Proteins*/genetics, Ultraviolet Rays ; DNA/chemistry ; DNA/genetics ; DNA/metabolism

Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1.

Authors: Senoussi I Mengoli V Cerana A et al.

Source: Nature communications [Nat Commun] 2025 Oct 27; Vol. 16 (1), pp. 9445. Date of Electronic Publication: 2025 Oct 27.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

MeSH Terms: Trinucleotide Repeat Expansion*/genetics , Exodeoxyribonucleases*/metabolism , Exodeoxyribonucleases*/genetics , Multifunctional Enzymes*/metabolism , Multifunctional Enzymes*/genetics , MutL Proteins*/metabolism , MutL Proteins*/genetics , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/genetics, Humans ; MutS Homolog 3 Protein/metabolism ; MutS Homolog 3 Protein/genetics ; Proliferating Cell Nuclear Antigen/metabolism ; Proliferating Cell Nuclear Antigen/genetics ; MutL Protein Homolog 1/metabolism ; MutL Protein Homolog 1/genetics ; DNA/metabolism ; DNA/genetics ; DNA/chemistry ; MutS Homolog 2 Protein/metabolism ; MutS Homolog 2 Protein/genetics ; DNA Polymerase III/metabolism ; Huntington Disease/genetics

Functional and structural insights into HCMV terminase accessory proteins pUL77 and pUL93.

Authors: Gourin C Di Meo F Delmon C et al.

Source: Journal of virology [J Virol] 2025 Oct 23; Vol. 99 (10), pp. e0117325. Date of Electronic Publication: 2025 Sep 16.

Publication Type: Journal Article

Journal Info: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE

MeSH Terms: Cytomegalovirus*/genetics , Cytomegalovirus*/drug effects , Cytomegalovirus*/enzymology , Viral Proteins*/genetics , Viral Proteins*/metabolism , Viral Proteins*/chemistry , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/chemistry, Humans ; Virus Replication/drug effects ; Antiviral Agents/pharmacology ; Cytomegalovirus Infections/virology ; Cytomegalovirus Infections/drug therapy ; Drug Resistance, Viral/genetics ; Quinazolines/pharmacology ; Acetates/pharmacology ; Mutation ; Nuclear Localization Signals/genetics ; Amino Acid Sequence

Engineered Cas12a-based one-tube detection of DNMT3A R882 H/C mutation in acute myeloid leukemia.

Authors: Liu Y Wu S Cao R et al.

Source: Biosensors & bioelectronics [Biosens Bioelectron] 2025 Oct 15; Vol. 286, pp. 117609. Date of Electronic Publication: 2025 May 19.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE

MeSH Terms: Leukemia, Myeloid, Acute*/genetics , Leukemia, Myeloid, Acute*/diagnosis , DNA (Cytosine-5-)-Methyltransferases*/genetics , CRISPR-Cas Systems*/genetics , Biosensing Techniques*/methods , Endodeoxyribonucleases*/genetics , Endodeoxyribonucleases*/chemistry, DNA Methyltransferase 3A ; Humans ; Mutation ; Bacterial Proteins ; CRISPR-Associated Proteins

CRISPR-Programmed CuO Nanocatalyst Release for Ultrasensitive Detection of Pathogens in Sterile Body Fluids.

Authors: Xiao J Hu X Chen H et al.

Source: Analytical chemistry [Anal Chem] 2025 Oct 14; Vol. 97 (40), pp. 22427-22435. Date of Electronic Publication: 2025 Oct 03.

Publication Type: Journal Article

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE

MeSH Terms: Copper*/chemistry , CRISPR-Associated Proteins*/metabolism , CRISPR-Associated Proteins*/chemistry , Endodeoxyribonucleases*/metabolism , Endodeoxyribonucleases*/chemistry , Body Fluids*/microbiology , Metal Nanoparticles*/chemistry , CRISPR-Cas Systems*, Humans ; Catalysis ; Benzidines/chemistry ; Limit of Detection ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/analysis ; Bacterial Proteins/metabolism ; Bacterial Proteins/chemistry ; Colorimetry