Výsledky vyhledávání - "Cell Culture Techniques/methods"

Bioanalytic Hybrid System Merging 3-Dimensional Cell Culture and Chromatographic Precision for Unprecedented Preclinical Insights in Molecular Imaging

Autoři: Pichler, Verena Schwingenschlögl-Maisetschläger, Verena Duman, Irem a další

Zdroj: J Nucl Med

Témata: 304007 Tissue engineering, Chromatography, Chromatography/methods, Cell Culture Techniques, Cell Culture Techniques/methods, 104020 Radiochemistry, Brief Communication, Molecular Imaging, 104020 Radiochemie, Positron Emission Tomography Computed Tomography, 304007 Tissue Engineering, Animals, Humans, Molecular Imaging/methods, Cell Culture Techniques, Three Dimensional, Three Dimensional/methods, Radiopharmaceuticals

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/40113219
https://ucrisportal.univie.ac.at/de/publications/c6aad915-1826-4367-857e-0b80f0b1e6a8
https://doi.org/10.2967/jnumed.124.269133

Reassessing Long-Term Cryopreservation Strategies for Improved Quality, Safety, and Clinical Use of Allogeneic Dermal Progenitor Cells

Autoři: Marjorie Flahaut Alexis Laurent Carla V. Fuenteslópez a další

Zdroj: The Journal of investigative dermatology, vol. 144, no. 10, pp. 2125-2135

Témata: Cryopreservation, Cryoprotective Agents, Cell Survival, Stem Cells, Cell Culture Techniques, Humans, Dermis, Fibroblasts, Regenerative Medicine, Cryopreservation/methods, Cryoprotective Agents/pharmacology, Regenerative Medicine/methods, Cells, Cultured, Stem Cells/cytology, Dermis/cytology, Cell Culture Techniques/methods, Clinical safety, Good Manufacturing Practices, Long-term storage, Progenitor cells

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39217536
https://ora.ox.ac.uk/objects/uuid:eabc220f-ec7f-4439-824c-5efa710da71f
https://doi.org/10.1016/j.jid.2024.06.1285
https://serval.unil.ch/resource/serval:BIB_66537DAEBF31.P001/REF.pdf
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_66537DAEBF317
https://serval.unil.ch/notice/serval:BIB_66537DAEBF31

Establishment of a patient-derived 3D in vitro meningioma model in xeno-free hydrogel for clinical applications

Autoři: Andersen, Mikkel Schou Nielsen, Aaraby Yoheswaran Wirenfeldt, Martin a další

Zdroj: Acta Neuropathol Commun
Acta Neuropathologica Communications, Vol 13, Iss 1, Pp 1-16 (2025)
Andersen, M S, Nielsen, A Y, Wirenfeldt, M, Petersen, J K, Møller, M W, Powell, C L, Castro, A, Herrgott, G, Mathiesen, T, Poulsen, C A, Olsen, B B, Boldt, H B, Pedersen, C B, Halle, B & Poulsen, F R 2025, ' Establishment of a patient-derived 3D in vitro meningioma model in xeno-free hydrogel for clinical applications ', Acta Neuropathologica Communications, vol. 13, no. 1, 81 . https://doi.org/10.1186/s40478-025-02008-w

Témata: 3D model, Organoid, Male, Meningioma/pathology, Cell Culture Techniques, Cell Culture Techniques/methods, Cell Line, In vitro model, Cell Line, Tumor, Meningeal Neoplasms, Humans, Cell Culture Techniques, Three Dimensional, Three Dimensional/methods, RC346-429, Tumor, DNA methylation, Research, Hydrogels, Middle Aged, Meningeal Neoplasms/pathology, Personalized medicine, Personalized model, Immunohistochemistry, Female, Neurology. Diseases of the nervous system, Meningioma

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/40269981
https://doaj.org/article/33f86677a9084afdb06b79ade54e5ea3
https://curis.ku.dk/ws/files/448987099/s40478-025-02008-w.pdf
https://portal.findresearcher.sdu.dk/da/publications/2988d64f-d716-4343-b050-e42bea81c2f7
https://doi.org/10.1186/s40478-025-02008-w

Process intensification of cultivated meat production through microcarrier addition strategy optimisation

Autoři: Bodiou, Vincent Cristini, Nicolas De Cristofaro, Lucrezia a další

Zdroj: Sci Rep
Scientific Reports, Vol 15, Iss 1, Pp 1-12 (2025)

Témata: Microcarrier, Bioreactor cultivation, Meat, Satellite Cells, Skeletal Muscle, Cells, Science, Cell Culture Techniques, Cell Culture Techniques/methods, Cell Count, Article, Bioreactors, Satellite cells, Animals, Bead-to-bead transfer, Skeletal Muscle/cytology metabolism, Cells, Cultured, Cell Proliferation, Cultured, Bioprocessing, Satellite Cells, Scale up, Medicine, Cattle, In Vitro Meat

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/40269015
https://doaj.org/article/c6edca91e0874b6393953259b8ded4f9

Culture Conditions for the Development of Equine Oocytes

Autoři: Baggio, Chiara Beitsma, Mabel M de Ruijter-Villani, Marta

Zdroj: Methods in Molecular Biology ISBN: 9781071646571

Témata: Sperm Injections, Intracytoplasmic/methods, Animals, Cell Culture Techniques/methods, In Vitro Oocyte Maturation Techniques/methods, Female, Horses, Cumulus Cells/cytology, Oocytes/cytology

Isolation and Generation of Osteoclasts

Autoři: Sharma, Neha Simic, Marija K Davies, Bethan K a další

Zdroj: Methods in Molecular Biology ISBN: 9781071643051

Témata: Mice, Cultured, Osteoclasts/cytology, Cells, Animals, Humans, Cell Culture Techniques/methods, Cell Differentiation, Cell Separation/methods

Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility

Autoři: Pamies, David Ekert, Jason Zurich, Marie-Gabrielle a další

Zdroj: Stem Cell Reports
Stem cell reports, vol. 19, no. 5, pp. 604-617

Témata: Quality Control, Organoids, Perspective, Cell Culture Techniques, Correction, Humans, Reproducibility of Results, Cell Culture Techniques/methods, Organoids/cytology, Microphysiological Systems, criteria, in vitro, microphysiological systems, organ on chip, organoids, quality control, recommendations, reproducibility, spheroids, standards, 500 - Naturwissenschaften und Mathematik

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/38670111
https://serval.unil.ch/resource/serval:BIB_1C641B815478.P001/REF.pdf
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_1C641B8154789
https://serval.unil.ch/notice/serval:BIB_1C641B815478

Simultaneous isolation and culture of endothelial colony-forming cells, endothelial cells and vascular smooth muscle cells from human umbilical cords

Autoři: Marie-Lotus Burger Steeve Menétrey Catherine Ponti a další

Zdroj: Mol Biol Rep
Molecular biology reports, vol. 52, no. 1, pp. 302

Témata: Male, Umbilical Veins, Myocytes, Smooth Muscle, Methodology, Cell Culture Techniques, Endothelial Cells, Cell Separation, Fetal Blood, Flow Cytometry, Humans, Umbilical Cord/cytology, Myocytes, Smooth Muscle/metabolism, Myocytes, Smooth Muscle/cytology, Muscle, Smooth, Vascular/cytology, Muscle, Smooth, Vascular/metabolism, Cell Separation/methods, Cells, Cultured, Fetal Blood/cytology, Human Umbilical Vein Endothelial Cells/metabolism, Endothelial Cells/metabolism, Endothelial Cells/cytology, Female, Cell Culture Techniques/methods, Flow Cytometry/methods, Umbilical Arteries/cytology, Pregnancy, Endothelial cell, Endothelial colony-forming cell, Human umbilical artery, Human umbilical cord blood, Human umbilical vein, Vascular smooth muscle cell, Muscle, Smooth, Vascular, Umbilical Arteries, Umbilical Cord, Human Umbilical Vein Endothelial Cells

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/40080230
https://serval.unil.ch/resource/serval:BIB_29AE573B4C2C.P001/REF.pdf
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_29AE573B4C2C3
https://serval.unil.ch/notice/serval:BIB_29AE573B4C2C

Supramolecular Additive Screening to Engineer Microfibrous Rafts for Expansion of Pluripotent Stem Cells in Dynamic Suspension

Autoři: Johnick F. van Sprang Jasper G. M. Aarts Boris Arts a další

Zdroj: Adv Healthc Mater

Témata: screening, Induced Pluripotent Stem Cells, biomaterial, Cell Culture Techniques, Cell Culture Techniques/methods, pluripotency, hiPSC, Induced Pluripotent Stem Cells/cytology, Cell Adhesion, Humans, electrospinning, supramolecular, Research Article, Cell Proliferation

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/40059619

Comparison of air-liquid interface transwell and airway organoid models for human respiratory virus infection studies

Autoři: Camilla T. Ekanger Nilima Dinesh Kumar Rosanne W. Koutstaal a další

Přispěvatelé: Camilla T. Ekanger Nilima Dinesh Kumar Rosanne W. Koutstaal a další

Zdroj: Front Immunol
Frontiers in Immunology, Vol 16 (2025)

Témata: Influenza A virus/physiology, Cells, Immunology, Cell Culture Techniques, Epithelial Cells/virology, Cell Culture Techniques/methods, Bronchi, Respiratory Mucosa, Virus Replication, Models, Biological, Respiratory Tract Infections/virology, influenza virus, Models, Influenza, Human, Humans, Bronchi/cytology, Respiratory Tract Infections, Cells, Cultured, Cultured, Organoids/virology, Cytokines/metabolism, Human/immunology, Epithelial Cells, RC581-607, Biological, respiratory tract, Influenza, Organoids, Respiratory Mucosa/virology, complex in vitro models, Influenza A virus, mucosal models, harmonization, Cytokines, Immunologic diseases. Allergy

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39981254
https://doaj.org/article/58f22a426f6a48649e6b3149113b6e98
https://rivm.openrepository.com/handle/10029/628397

Using a Supramolecular Approach to Engineer Modular Hydrogel Platforms for Culturing Protoplasts – from General Tissue Engineering to Cellular Agriculture

Autoři: Rovers, Maritza M. Slootweg, Erik J. Los, Ferdinand C.O. a další

Zdroj: Rovers, M M, Slootweg, E J, Los, F C O & Dankers, P Y W 2025, 'Using a Supramolecular Approach to Engineer Modular Hydrogel Platforms for Culturing Protoplasts – from General Tissue Engineering to Cellular Agriculture', Advanced Biology, vol. 9, no. 11, e00690. https://doi.org/10.1002/adbi.202400690

Témata: cell wall regeneration, cellular agriculture, protoplast regeneration, supramolecular hydrogels, tobacco BY-2, Tissue Engineering/methods, Cell Culture Techniques/methods, Protoplasts/cytology, Hydrogels/chemistry

Popis souboru: application/pdf

Relation: info:eu-repo/semantics/altIdentifier/pmid/40464167; info:eu-repo/semantics/altIdentifier/pissn/2701-0198

Dostupnost: https://research.tue.nl/en/publications/3c3cfee8-ce0a-4af8-bf2d-da0b8335817f
https://doi.org/10.1002/adbi.202400690
https://pure.tue.nl/ws/files/371832045/Advanced_Biology_-_2025_-_Rovers_-_Using_a_Supramolecular_Approach_to_Engineer_Modular_Hydrogel_Platforms_for_Culturing.pdf
https://www.scopus.com/pages/publications/105007543833

A call for standardization:evaluating different methodologies to induce in vitro foreign body giant cell formation for biomaterials research and design

Autoři: Conner, Thijs S. Baaijens, Frank P.T. Bouten, Carlijn V.C. a další

Zdroj: Conner, T S, Baaijens, F P T, Bouten, C V C, Angeloni, L & Smits, A I P M 2025, 'A call for standardization : evaluating different methodologies to induce in vitro foreign body giant cell formation for biomaterials research and design', Acta Biomaterialia, vol. 194, pp. 20-37. https://doi.org/10.1016/j.actbio.2025.01.026

Témata: Foreign body giant cells (FBGCS), Macrophage fusion, Immune response, In vitro, Cell-material interaction, Multinucleated giant cells, Foreign body response (FBR), Macrophage, Cell Culture Techniques/methods, Humans, Materials Testing, Foreign-Body Reaction/pathology, Giant Cells, Foreign-Body/metabolism, Animals, Biocompatible Materials

Popis souboru: application/pdf

Relation: info:eu-repo/semantics/altIdentifier/pmid/39826854; info:eu-repo/semantics/altIdentifier/pissn/1742-7061

Dostupnost: https://research.tue.nl/en/publications/b0de863b-a453-466d-b022-7b31bbfc72e7
https://doi.org/10.1016/j.actbio.2025.01.026
https://pure.tue.nl/ws/files/353736792/1-s2.0-S1742706125000339-main.pdf
https://www.scopus.com/pages/publications/85216020052

Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation

Autoři: Blotenburg, Marloes Suurenbroek, Lianne Bax, Danique a další

Témata: Animals, Mice, Cell Differentiation, Mouse Embryonic Stem Cells/cytology, DNA Methylation, Cell Culture Techniques/methods, Epigenesis, Genetic, Cells, Cultured, Journal Article

Popis souboru: application/pdf

Relation: https://dspace.library.uu.nl/handle/1874/460988

Dostupnost: https://dspace.library.uu.nl/handle/1874/460988

Comparative analysis of cryopreserved adipose stem cells expanded in hollow fiber bioreactor versus conventional tissue culture flasks

Autoři: Guoqiang Ren Morten Brøndum Sørensen Simone Riis Porsborg a další

Zdroj: Sci Rep
Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
Ren, G, Sørensen, M B, Porsborg, S R, Fink, T, Zachar, V & Peng, Q 2024, 'Comparative analysis of cryopreserved adipose stem cells expanded in hollow fiber bioreactor versus conventional tissue culture flasks', Scientific Reports, vol. 14, no. 1, 31853. https://doi.org/10.1038/s41598-024-83255-0

Témata: Adipose-derived stem cells, Cryopreservation, Cryopreservation/methods, Science, Stem Cells, Cell Culture Techniques, Cell Culture Techniques/methods, Hollow fiber bioreactors, Tissue Culture Techniques/methods, Cell Differentiation, Article, Tissue Culture Techniques, Tissue culture polystyrene, Bioreactors, Adipose Tissue, Subpopulations, Stem Cells/cytology, Medicine, Humans, Adipose Tissue/cytology, Flow cytometry, Cells, Cultured, Cell Proliferation

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39738501
https://doaj.org/article/3b122665d1fe478ca71692f7bec88194

Clinical-scale, modular manufacturing of tumor-reactive TILs using a closed and automated culture system

Autoři: Christina Völzke Lisa Ehrhardt Laura Fischer a další

Zdroj: Front Immunol
Frontiers in Immunology, Vol 15 (2024)
Frontiers in immunology, vol. 15, pp. 1483254

Témata: 0301 basic medicine, 0303 health sciences, Immunology, Cell Culture Techniques, RC581-607, GMP compliant cell manufacturing, Immunotherapy, Adoptive, REP, CliniMACS Prodigy, Tumor Necrosis Factor Receptor Superfamily, Member 9, 03 medical and health sciences, Lymphocytes, Tumor-Infiltrating, Lymphocytes, Tumor-Infiltrating/immunology, Lymphocytes, Tumor-Infiltrating/metabolism, Humans, Cell Culture Techniques/methods, Melanoma/immunology, Tumor Necrosis Factor Receptor Superfamily, Member 9/metabolism, Tumor Necrosis Factor Receptor Superfamily, Member 9/immunology, Immunotherapy, Adoptive/methods, Neoplasms/immunology, CD137, TILs, automation, tumor reactive T cells, Neoplasms, Immunologic diseases. Allergy, Melanoma

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39717766
https://doaj.org/article/0c55473bf87947b58a151a1e92727b8e
https://serval.unil.ch/resource/serval:BIB_0CA15617B24B.P001/REF.pdf
https://serval.unil.ch/notice/serval:BIB_0CA15617B24B
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_0CA15617B24B1

Microcavity-assisted cloning (MAC) of hard-to-clone HepG2 cell lines: cloning made easy

Autoři: Mlakar, Vid Lesne, Laurence Vossio, Stefania a další

Zdroj: BMC Biotechnol
BMC Biotechnology, Vol 24, Iss 1, Pp 1-7 (2024)

Témata: HepG2, Cloning, Molecular / methods, Cell Culture Techniques, Gene Knockout Techniques, Glutathione Transferase / metabolism, Humans, Single cell, Cloning, Molecular, Glutathione Transferase, Sorting, Research, Hard-to-clone, Hep G2 Cells, Clone Cells, Glutathione Transferase / genetics, CRISPR-Cas Systems / genetics, Gene Knockout Techniques / methods, CRISPR-Cas Systems, Cell line, Microcavity, TP248.13-248.65, Cell Culture Techniques / methods, Cloning, Biotechnology

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/39407195
https://doaj.org/article/006d1ac0135d442aa4af5f3552f3cd67

RAW 264.7 Cell Culture Protocol

Autoři: Subirana Slotos, Robert-Josep

Témata: Cell Culture Techniques/classification, Cell Culture Techniques, Cell Culture Techniques/instrumentation, Primary Cell Culture/trends, Cell Culture Techniques/methods, Cell Culture Techniques/trends, Cell Culture Techniques/history, Cell Culture Techniques/standards

Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation

Autoři: Marloes Blotenburg Lianne Suurenbroek Danique Bax a další

Zdroj: PLoS One
PLoS ONE, Vol 20, Iss 3, p e0317309 (2025)
PLoS One, 20, 3, pp. 1-20
Blotenburg, M, Suurenbroek, L, Bax, D, de Visser, J, Bhardwaj, V, Braccioli, L, de Wit, E, van Boxtel, A, Marks, H & Zeller, P 2025, 'Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation', PLOS ONE, vol. 20, no. 3, e0317309. https://doi.org/10.1371/journal.pone.0317309

Témata: Science, Cell Culture Techniques, Cell Culture Techniques/methods, Cell Differentiation, Mouse Embryonic Stem Cells, DNA Methylation, Epigenesis, Genetic, Mice, Journal Article, Medicine, Animals, Molecular Biology, Mouse Embryonic Stem Cells/cytology, Cells, Cultured, Research Article

Popis souboru: application/pdf

Přístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/40138371
https://doaj.org/article/a303e516620f4a6abd85b06bce1d85ff
https://dspace.library.uu.nl/handle/1874/460988
https://repository.ubn.ru.nl//bitstream/handle/2066/319839/319839.pdf
https://hdl.handle.net/2066/319839

Accelerated production of human epithelial organoids in a miniaturized spinning bioreactor

Autoři: Ye, Shicheng Marsee, Ary van Tienderen, Gilles S a další

Přispěvatelé: Ye, Shicheng Marsee, Ary van Tienderen, Gilles S a další

Témata: Bioreactors, Cell Culture Techniques/methods, Cell Differentiation, Cell Proliferation, Cells, Cultured, Epithelial Cells/cytology, Hepatocytes/cytology, Humans, Liver/cytology, Miniaturization, Organoids/metabolism, Pancreas/cytology, Journal Article

Popis souboru: application/pdf

Relation: https://dspace.library.uu.nl/handle/1874/459129

Dostupnost: https://dspace.library.uu.nl/handle/1874/459129

Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture

Autoři: Lingard, Eliana Dong, Siyuan Hoyle, Anna a další

Zdroj: Lingard, E, Dong, S, Hoyle, A, Appleton, E, Hales, A, Skaria, E, Lawless, C, Taylor-Hearn, I, Saadati, S, Chu, Q, Miller, A F, Domingos, M, Saiani, A, Swift, J & Gilmore, A P 2024, 'Optimising a self-assembling peptide hydrogel as a Matrigel alternative for 3-dimensional mammary epithelial cell culture', Biomaterials Advances, vol. 160, 213847. https://doi.org/10.1016/j.bioadv.2024.213847

Témata: Laminin/pharmacology, Hydrogels/chemistry, Drug Combinations, Proteoglycans/pharmacology, Collagen/chemistry, Peptides/pharmacology, Epithelial Cells/drug effects, Humans, Female, Cell Culture Techniques, Three Dimensional/methods, Cell Survival/drug effects, Biocompatible Materials/chemistry, Mammary Glands, Human/cytology, Organoids/drug effects, Cell Culture Techniques/methods

Dostupnost: https://research.manchester.ac.uk/en/publications/2a95a82d-fbbc-4726-b53a-7191a46db7a7
https://doi.org/10.1016/j.bioadv.2024.213847
https://pubmed.ncbi.nlm.nih.gov/38657288/