Suchergebnisse - "CLONING"

MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems.

Autoren: Gillespie WB 3rd Zhang Y Ruiz OE et al.

Quelle: Development (Cambridge, England) [Development] 2025 Nov 15; Vol. 152 (22). Date of Electronic Publication: 2025 Nov 13.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 8701744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9129 (Electronic) Linking ISSN: 09501991 NLM ISO Abbreviation: Development Subsets: MEDLINE

MeSH-Schlagworte: Cloning, Molecular*/methods , Vertebrates*/genetics, Animals ; Zebrafish/genetics ; Genetic Vectors/genetics ; Mice ; Plasmids/genetics ; Humans ; Transposases ; Chick Embryo ; HEK293 Cells ; Transgenes/genetics ; Gene Transfer Techniques

Co-expression enhances cloning efficiency and protein production in CHO cells.

Autoren: Fang Y Shi L Wang Y et al.

Quelle: Protein expression and purification [Protein Expr Purif] 2025 Oct; Vol. 234, pp. 106763. Date of Electronic Publication: 2025 Jun 18.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Academic Press Country of Publication: United States NLM ID: 9101496 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0279 (Electronic) Linking ISSN: 10465928 NLM ISO Abbreviation: Protein Expr Purif Subsets: MEDLINE

MeSH-Schlagworte: Insulin-Like Growth Factor I*/genetics , Insulin-Like Growth Factor I*/biosynthesis , Insulin-Like Growth Factor I*/metabolism , Recombinant Proteins*/genetics , Recombinant Proteins*/biosynthesis , Cloning, Molecular*/methods, Animals ; CHO Cells ; Cricetulus ; Cell Proliferation ; Gene Expression ; Cricetinae

Individual variations in buffalo somatic cell cloning efficiency: Synergistic regulation by mitochondria and chromatin remodeling.

Autoren: Yang X Gan H Bai Y et al.

Quelle: Theriogenology [Theriogenology] 2025 Dec; Vol. 248, pp. 117602. Date of Electronic Publication: 2025 Jul 25.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE

MeSH-Schlagworte: Buffaloes*/embryology , Buffaloes*/genetics , Buffaloes*/physiology , Mitochondria*/physiology , Mitochondria*/ultrastructure , Chromatin Assembly and Disassembly*/physiology , Cloning, Organism*/veterinary, Animals ; Nuclear Transfer Techniques/veterinary ; Fibroblasts/physiology ; Fibroblasts/ultrastructure ; Female ; Male ; Fertilization in Vitro/veterinary

DUXA regulates the early development of porcine cloned embryos.

Autoren: Shi Z Yan Y Zhu R et al.

Quelle: Theriogenology [Theriogenology] 2025 Nov; Vol. 247, pp. 117563. Date of Electronic Publication: 2025 Jul 05.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE

MeSH-Schlagworte: Cloning, Organism*/veterinary , Embryonic Development*/genetics , Embryonic Development*/physiology , Gene Expression Regulation, Developmental*/physiology , Homeodomain Proteins*/genetics , Homeodomain Proteins*/metabolism , Transcription Factors*/genetics , Transcription Factors*/metabolism, Animals ; Swine/embryology ; Swine/genetics ; Nuclear Transfer Techniques/veterinary ; Embryo, Mammalian ; Blastocyst/physiology ; Female ; CRISPR-Cas Systems

A versatile platform for combinatorial antibody library cloning and NGS-based quality control with high accuracy.

Autoren: Zhang Z D'Hondt S Van der Kant R et al.

Quelle: Nucleic acids research [Nucleic Acids Res] 2025 Oct 14; Vol. 53 (19).

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

MeSH-Schlagworte: Cloning, Molecular*/methods , High-Throughput Nucleotide Sequencing*/methods , Single-Domain Antibodies*/genetics , Single-Domain Antibodies*/chemistry , Peptide Library*, Quality Control ; Immunoglobulin Fab Fragments/genetics ; Immunoglobulin Fab Fragments/chemistry ; Polymerase Chain Reaction ; Complementarity Determining Regions/genetics ; Humans ; Gene Library

Efficient Somatic Cell Nuclear Transfer by Overcoming Both Pre- and Post-Implantation Epigenetic Barriers.

Autoren: Li Y Sun S Xu Y et al.

Quelle: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2025 Oct; Vol. 12 (37), pp. e04669. Date of Electronic Publication: 2025 Jul 08.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE

MeSH-Schlagworte: Nuclear Transfer Techniques* , Epigenesis, Genetic*/genetics , Cloning, Organism*/methods , Embryonic Development*/genetics, Animals ; Mice ; Female ; Cellular Reprogramming/genetics ; Jumonji Domain-Containing Histone Demethylases/genetics

High Expression of ZFP42 Improves Early Development of Pig Embryos Produced by Handmade Cloning.

Autoren: Liu T Wu Y Li L et al.

Quelle: Cellular reprogramming [Cell Reprogram] 2024 Apr; Vol. 26 (2), pp. 57-66. Date of Electronic Publication: 2024 Apr 10.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101528176 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2152-4998 (Electronic) Linking ISSN: 21524971 NLM ISO Abbreviation: Cell Reprogram Subsets: MEDLINE

MeSH-Schlagworte: Nuclear Transfer Techniques* , Cloning, Organism*/methods, Swine ; Animals ; Embryonic Development/physiology ; Transcriptome ; Cloning, Molecular ; Blastocyst/metabolism

Overlap Extension Construction and Direct Cloning of Loop-Out Prone DNA Expression Cassettes in Pichia pastoris: 75 k-γ-Secalin from Secale cereale (rye) as a Case Study.

Autoren: Büchner K Pichler C Kerpes R et al.

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2697, pp. 205-215.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Cloning, Molecular*/methods , Saccharomycetales*/genetics, Polymerase Chain Reaction/methods ; Plasmids/genetics ; Gene Expression ; Transformation, Genetic

SCR Organism: Komagataella pastoris

Cloning for the Twenty-First Century and Its Place in Endangered Species Conservation.

Autoren: Cowl VB Comizzoli P Appeltant R et al.

Quelle: Annual review of animal biosciences [Annu Rev Anim Biosci] 2024 Feb 15; Vol. 12, pp. 91-112. Date of Electronic Publication: 2023 Nov 21.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Annual Reviews Country of Publication: United States NLM ID: 101614024 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-8110 (Electronic) Linking ISSN: 21658102 NLM ISO Abbreviation: Annu Rev Anim Biosci Subsets: MEDLINE

MeSH-Schlagworte: Endangered Species* , Cloning, Organism*/veterinary , Cloning, Organism*/methods, Animals ; Nuclear Transfer Techniques/veterinary ; Fishes/genetics ; Cloning, Molecular

Flexible oocyte manipulation with delayed maturation and improved somatic cell nuclear transfer efficiency using induced pluripotent stem cells.

Autoren: de Aguiar LH Lee YL Abdelhady AW et al.

Quelle: Theriogenology [Theriogenology] 2026 Feb 01; Vol. 251, pp. 117754. Date of Electronic Publication: 2025 Nov 13.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE

MeSH-Schlagworte: Nuclear Transfer Techniques*/veterinary , Oocytes*/physiology , Induced Pluripotent Stem Cells*/physiology , In Vitro Oocyte Maturation Techniques*/veterinary , In Vitro Oocyte Maturation Techniques*/methods , Cloning, Organism*/veterinary , Cloning, Organism*/methods, Animals ; Cattle ; Female ; Embryonic Development ; Parthenogenesis

Pichia pastoris Expression Plasmid Assembly Using Type IIS Restriction Enzymes.

Autoren: Grebe ACI Schallengruber LJ Gomez CMA et al.

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2697, pp. 179-189.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Plasmids*/genetics , Cloning, Molecular*/methods , Deoxyribonucleases, Type II Site-Specific*/metabolism , Deoxyribonucleases, Type II Site-Specific*/genetics , Saccharomycetales*/genetics, Gene Expression ; Genetic Vectors/genetics ; Recombinant Proteins/genetics

SCR Organism: Komagataella pastoris

Map-Based Cloning of Apomixis Genes in Dandelion (Taraxacum officinale s.l.).

Autoren: van Dijk PJ Radoeva T Op den Camp RHM et al.

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2987, pp. 195-207.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Taraxacum*/genetics , Apomixis*/genetics , Cloning, Molecular*/methods , Genes, Plant* , Chromosome Mapping*/methods

A Review of DNA Restriction-Free Overlapping Sequence Cloning Techniques for Synthetic Biology.

Autoren: Bomfiglio IF Mendes ISM Bonatto D

Quelle: Biotechnology journal [Biotechnol J] 2025 Jul; Vol. 20 (7), pp. e70084.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: Wiley-VCH Verlag Country of Publication: Germany NLM ID: 101265833 Publication Model: Print Cited Medium: Internet ISSN: 1860-7314 (Electronic) Linking ISSN: 18606768 NLM ISO Abbreviation: Biotechnol J Subsets: MEDLINE

MeSH-Schlagworte: Synthetic Biology*/methods , Cloning, Molecular*/methods , DNA*/genetics, Genetic Engineering/methods

Cloning of MFα Secretion Signal Variants for Protein Secretion by Pichia pastoris.

Autoren: Merkaš M Pantelakis OI Emmerstorfer-Augustin A

Quelle: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2697, pp. 241-255.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH-Schlagworte: Protein Sorting Signals*/genetics , Cloning, Molecular*/methods , Mating Factor*/genetics , Mating Factor*/metabolism , Saccharomycetales*/genetics , Saccharomycetales*/metabolism , Pichia*/genetics , Pichia*/metabolism , Fungal Proteins*/genetics , Fungal Proteins*/metabolism, Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Genetic Vectors/genetics ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Protein Transport

SCR Organism: Komagataella pastoris

Enhancing embryonic and full-term development during mouse cloning through post-activation treatment with JNJ-7706621.

Autoren: Kang HG Kim MJ Yun JH et al.

Quelle: Theriogenology [Theriogenology] 2025 Sep 15; Vol. 244, pp. 117475. Date of Electronic Publication: 2025 May 08.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE

MeSH-Schlagworte: Embryonic Development*/drug effects , Cloning, Organism*/veterinary , Cloning, Organism*/methods , Nuclear Transfer Techniques*/veterinary, Animals ; Mice ; Female ; Embryo Culture Techniques/veterinary ; Cytochalasin B/pharmacology ; Embryo, Mammalian/drug effects ; Parthenogenesis

Effects of Donor Cell Type on Developmental Competence of Canine-Porcine Interspecies Cloned Embryos.

Autoren: Yan Y Li Z Zhang G et al.

Quelle: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2025 Sep; Vol. 60 (9), pp. e70121.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE

MeSH-Schlagworte: Nuclear Transfer Techniques*/veterinary , Dogs* , Sus scrofa* , Cloning, Organism*/methods, Animals ; Tissue Culture Techniques ; Apoptosis ; Fibroblasts ; Mesenchymal Stem Cells ; Pluripotent Stem Cells ; Morphogenesis ; Genes, Homeobox ; Ear

Effect of porcine oviductal fluid-derived extracellular vesicle supplementation on in vitro embryonic developmental competence and the production efficiency of cloned pigs.

Autoren: Kim JH Shim J Ko N et al.

Quelle: Theriogenology [Theriogenology] 2025 Aug; Vol. 242, pp. 117442. Date of Electronic Publication: 2025 Apr 11.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE

MeSH-Schlagworte: Extracellular Vesicles* , Cloning, Organism*/veterinary , Embryonic Development* , Fallopian Tubes* , Oviducts*, Animals ; Female ; Swine/embryology ; Nuclear Transfer Techniques/veterinary ; Embryo Culture Techniques/veterinary

Brief guide to gene cloning.

Autoren: Hong W Ha SG Kwon HC et al.

Quelle: Molecules and cells [Mol Cells] 2025 Aug; Vol. 48 (8), pp. 100234. Date of Electronic Publication: 2025 May 29.

Publikationsart: Letter; Review

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 9610936 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0219-1032 (Electronic) Linking ISSN: 10168478 NLM ISO Abbreviation: Mol Cells Subsets: MEDLINE

MeSH-Schlagworte: Cloning, Molecular*/methods, Animals ; Humans ; DNA/genetics

Birth of India's first cloned cattle and analysis of its health and reproduction status: A case study.

Autoren: Selokar NL Singh MK Tripathi G et al.

Quelle: Scientific reports [Sci Rep] 2025 Jul 24; Vol. 15 (1), pp. 26957. Date of Electronic Publication: 2025 Jul 24.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH-Schlagworte: Cloning, Organism*/veterinary , Cloning, Organism*/methods , Nuclear Transfer Techniques*/veterinary , Reproduction*, Animals ; Cattle ; Female ; India ; Pregnancy ; Embryo Transfer/veterinary ; Oocytes/cytology ; Blastocyst/cytology ; Microsatellite Repeats

Development and Application of an ACQUIRE Method for Direct Cloning of Superlarge Biosynthetic Gene Cluster.

Autoren: Wang W Ma N Xu T et al.

Quelle: ACS synthetic biology [ACS Synth Biol] 2025 Jul 18; Vol. 14 (7), pp. 2480-2487. Date of Electronic Publication: 2025 Jun 16.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

MeSH-Schlagworte: Multigene Family*/genetics , Cloning, Molecular*/methods, Polyketide Synthases/genetics ; Polyketide Synthases/metabolism ; Amycolatopsis/genetics ; Amycolatopsis/metabolism ; Rifamycins/biosynthesis