Suchergebnisse - "CENTRAL nervous system infections"

Impact of metagenomic sequencing on clinical outcomes in patients with suspected central nervous system infections: a retrospective case-control study.

Autoren: An W Zhang Y Liu Y et al.

Quelle: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2025 Nov 07; Vol. 15, pp. 1677092. Date of Electronic Publication: 2025 Nov 07 (Print Publication: 2025).

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE

MeSH-Schlagworte: Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/microbiology , Central Nervous System Infections*/cerebrospinal fluid , Central Nervous System Infections*/mortality , Metagenomics*/methods, Humans ; Retrospective Studies ; Male ; Female ; Middle Aged ; Case-Control Studies ; Aged ; Adult ; Treatment Outcome ; Hospital Mortality

Cost-effectiveness analysis of metagenomic next-generation sequencing versus traditional bacterial cultures for postoperative central nervous system infections in critical care settings: a prospective pilot study.

Autoren: Tian Y Xu N Chen Y et al.

Quelle: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2025 Oct 28; Vol. 15, pp. 1710412. Date of Electronic Publication: 2025 Oct 28 (Print Publication: 2025).

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE

MeSH-Schlagworte: Cost-Benefit Analysis* , High-Throughput Nucleotide Sequencing*/economics , High-Throughput Nucleotide Sequencing*/methods , Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/microbiology , Central Nervous System Infections*/economics , Metagenomics*/economics , Metagenomics*/methods , Critical Care*/economics , Bacteria*/genetics , Bacteria*/isolation & purification , Bacteria*/classification , Postoperative Complications*/microbiology , Postoperative Complications*/diagnosis , Bacteriological Techniques*/economics , Bacteriological Techniques*/methods, Humans ; Pilot Projects ; Prospective Studies ; Female ; Male ; Middle Aged ; Aged ; Adult ; Intensive Care Units ; China ; Cost-Effectiveness Analysis

Causative pathogens and predictors of unfavourable outcomes in central nervous system infections in resource-limited settings.

Autoren: Dong DV Sang VV Hoan NX et al.

Quelle: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2025 Oct; Vol. 159, pp. 108002. Date of Electronic Publication: 2025 Aug 05.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: Canada NLM ID: 9610933 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3511 (Electronic) Linking ISSN: 12019712 NLM ISO Abbreviation: Int J Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: Central Nervous System Infections*/microbiology , Central Nervous System Infections*/mortality , Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/virology , Central Nervous System Infections*/epidemiology, Humans ; Male ; Female ; Adult ; Middle Aged ; Young Adult ; Vietnam/epidemiology ; Adolescent ; Aged ; Klebsiella pneumoniae/isolation & purification ; Risk Factors ; Acinetobacter baumannii/isolation & purification ; Resource-Limited Settings

Varicella-Zoster Virus Clades Circulating Among Children With Varicella and Adults With Central Nervous System Infection in Japan.

Autoren: Higashimoto Y Hattori F Kawamura Y et al.

Quelle: Journal of medical virology [J Med Virol] 2025 Dec; Vol. 97 (12), pp. e70750.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE

MeSH-Schlagworte: Herpesvirus 3, Human*/genetics , Herpesvirus 3, Human*/classification , Herpesvirus 3, Human*/isolation & purification , Chickenpox*/epidemiology , Chickenpox*/virology , Central Nervous System Infections*/virology , Central Nervous System Infections*/epidemiology , Varicella Zoster Virus Infection*/epidemiology , Varicella Zoster Virus Infection*/virology, Humans ; Japan/epidemiology ; Male ; Child ; Female ; Adult ; Child, Preschool ; Adolescent ; DNA, Viral/genetics ; DNA, Viral/cerebrospinal fluid ; Infant ; Middle Aged ; Young Adult ; Aged ; Phylogeny ; Genotype ; Herpes Zoster/epidemiology ; Herpes Zoster/virology

Clinical metagenomic next-generation sequencing test for diagnosis of central nervous system infections in ICU: a multicenter retrospective study.

Autoren: Liu J Dong Y Huang Y et al.

Quelle: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2025 Dec; Vol. 161, pp. 108094. Date of Electronic Publication: 2025 Sep 30.

Publikationsart: Journal Article; Multicenter Study

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: Canada NLM ID: 9610933 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3511 (Electronic) Linking ISSN: 12019712 NLM ISO Abbreviation: Int J Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , Metagenomics*/methods , Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/microbiology , Central Nervous System Infections*/cerebrospinal fluid, Humans ; Retrospective Studies ; Intensive Care Units ; Female ; Male ; Middle Aged ; Aged ; Adult ; Sensitivity and Specificity ; Bacteria/genetics ; Bacteria/isolation & purification ; Aged, 80 and over ; Young Adult

Utility and performance of cerebrospinal fluid cytology in discriminating central nervous system infections and brain tumors.

Autoren: Su M Duan H Lei Q et al.

Quelle: Journal of neuro-oncology [J Neurooncol] 2025 Dec; Vol. 175 (3), pp. 1343-1353. Date of Electronic Publication: 2025 Sep 10.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Springer Country of Publication: United States NLM ID: 8309335 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-7373 (Electronic) Linking ISSN: 0167594X NLM ISO Abbreviation: J Neurooncol Subsets: MEDLINE

MeSH-Schlagworte: Brain Neoplasms*/cerebrospinal fluid , Brain Neoplasms*/diagnosis , Central Nervous System Infections*/cerebrospinal fluid , Central Nervous System Infections*/diagnosis , Cerebrospinal Fluid*/cytology, Humans ; Female ; Male ; Middle Aged ; Diagnosis, Differential ; Adult ; Aged ; Cytodiagnosis/methods ; Young Adult ; ROC Curve ; Nomograms ; Adolescent ; Child ; Retrospective Studies

Vancomycin Penetration in Brain Extracellular Fluid of Patients with Post-Surgical Central Nervous System Infections: An Exploratory Study.

Autoren: Žukaitienė S Bareikis K Stankevičiūtė S et al.

Quelle: Medicina (Kaunas, Lithuania) [Medicina (Kaunas)] 2025 Nov 05; Vol. 61 (11). Date of Electronic Publication: 2025 Nov 05.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: MDPI Country of Publication: Switzerland NLM ID: 9425208 Publication Model: Electronic Cited Medium: Internet ISSN: 1648-9144 (Electronic) Linking ISSN: 1010660X NLM ISO Abbreviation: Medicina (Kaunas) Subsets: MEDLINE

MeSH-Schlagworte: Vancomycin*/therapeutic use , Vancomycin*/pharmacokinetics , Extracellular Fluid*/chemistry , Extracellular Fluid*/metabolism , Extracellular Fluid*/drug effects , Anti-Bacterial Agents*/therapeutic use , Anti-Bacterial Agents*/pharmacokinetics , Central Nervous System Infections*/drug therapy , Central Nervous System Infections*/etiology , Brain*/metabolism , Postoperative Complications*/drug therapy, Humans ; Male ; Middle Aged ; Female ; Aged ; Microdialysis/methods ; Adult ; Blood-Brain Barrier

Clinical performance of metagenomic next-generation sequencing in the diagnosis of Epstein-Barr virus central nervous system infections.

Autoren: Yao X Huang Y Yuan L et al.

Quelle: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2025 Nov; Vol. 44 (11), pp. 2623-2634. Date of Electronic Publication: 2025 Jul 26.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , Epstein-Barr Virus Infections*/diagnosis , Epstein-Barr Virus Infections*/virology , Epstein-Barr Virus Infections*/cerebrospinal fluid , Herpesvirus 4, Human*/genetics , Herpesvirus 4, Human*/isolation & purification , Metagenomics*/methods , Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/virology, Humans ; Male ; Female ; Adult ; Middle Aged ; DNA, Viral/genetics ; Viral Load ; Young Adult ; Aged ; Adolescent ; Sensitivity and Specificity ; Child ; Polymerase Chain Reaction

The comparison of three regimens of polymyxin B for the treatment of central nervous system infections caused by carbapenem-resistant bacteria: A retrospective cohort study.

Autoren: Leng X Xia L Zhou H et al.

Quelle: Journal of infection and public health [J Infect Public Health] 2025 Nov; Vol. 18 (11), pp. 102903. Date of Electronic Publication: 2025 Jul 16.

Publikationsart: Journal Article; Comparative Study

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: England NLM ID: 101487384 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-035X (Electronic) Linking ISSN: 18760341 NLM ISO Abbreviation: J Infect Public Health Subsets: MEDLINE

MeSH-Schlagworte: Polymyxin B*/administration & dosage , Polymyxin B*/therapeutic use , Anti-Bacterial Agents*/administration & dosage , Anti-Bacterial Agents*/therapeutic use , Carbapenems*/pharmacology , Central Nervous System Infections*/drug therapy , Central Nervous System Infections*/microbiology, Humans ; Retrospective Studies ; Male ; Female ; Middle Aged ; Aged ; Treatment Outcome ; Adult ; Aged, 80 and over

Central nervous system infection caused by Mycoplasma hominis in an adult: a case report and literature review.

Autoren: Gao X Guo Y Luo P et al.

Quelle: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2025 Nov; Vol. 44 (11), pp. 2695-2703. Date of Electronic Publication: 2025 Aug 11.

Publikationsart: Journal Article; Case Reports; Systematic Review; Review

Info zur Zeitschrift: Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: Mycoplasma hominis*/isolation & purification , Mycoplasma hominis*/genetics , Mycoplasma Infections*/drug therapy , Mycoplasma Infections*/diagnosis , Mycoplasma Infections*/microbiology , Central Nervous System Infections*/microbiology , Central Nervous System Infections*/drug therapy , Central Nervous System Infections*/diagnosis, Humans ; Male ; Middle Aged ; Anti-Bacterial Agents/therapeutic use ; Retrospective Studies ; Female ; Meningitis, Bacterial/drug therapy ; Meningitis, Bacterial/microbiology ; Meningitis, Bacterial/diagnosis ; Brain Abscess/microbiology ; Aged ; Adult ; Young Adult ; Moxifloxacin/therapeutic use

Efficacy analysis of intraventricular/intrathecal polymyxin B sulphate injections in the treatment of Post-neurosurgical CNS infections in neurocritical ill patients: a prospective observational study.

Autoren: Song J Yuan HY Wang MH et al.

Quelle: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2025 Nov; Vol. 44 (11), pp. 2749-2759. Date of Electronic Publication: 2025 Aug 15.

Publikationsart: Journal Article; Observational Study

Info zur Zeitschrift: Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: Polymyxin B*/administration & dosage , Polymyxin B*/therapeutic use , Anti-Bacterial Agents*/administration & dosage , Anti-Bacterial Agents*/therapeutic use , Central Nervous System Infections*/drug therapy , Central Nervous System Infections*/microbiology , Neurosurgical Procedures*/adverse effects, Humans ; Male ; Female ; Prospective Studies ; Middle Aged ; Adult ; Injections, Intraventricular ; Aged ; Treatment Outcome ; Critical Illness ; Injections, Spinal ; Acinetobacter Infections/drug therapy ; Klebsiella pneumoniae/drug effects

Linezolid use in post-neurosurgical central nervous system infections: A systematic review.

Autoren: Baulier C Le Dentu N Lozouet M et al.

Quelle: Neurosurgical review [Neurosurg Rev] 2025 Oct 16; Vol. 48 (1), pp. 714. Date of Electronic Publication: 2025 Oct 16.

Publikationsart: Journal Article; Systematic Review; Review

Info zur Zeitschrift: Publisher: Springer Berlin Heidelberg Country of Publication: Germany NLM ID: 7908181 Publication Model: Electronic Cited Medium: Internet ISSN: 1437-2320 (Electronic) Linking ISSN: 03445607 NLM ISO Abbreviation: Neurosurg Rev Subsets: MEDLINE

MeSH-Schlagworte: Linezolid*/therapeutic use , Anti-Bacterial Agents*/therapeutic use , Central Nervous System Infections*/drug therapy , Central Nervous System Infections*/etiology , Neurosurgical Procedures*/adverse effects , Postoperative Complications*/drug therapy, Humans

Prenatal and Neonatal Infections of the Central Nervous System.

Autoren: de Vries LS Bekker V Steggerda SJ

Quelle: Pediatric clinics of North America [Pediatr Clin North Am] 2025 Oct; Vol. 72 (5), pp. 825-842. Date of Electronic Publication: 2025 Jul 23.

Publikationsart: Journal Article; Review

Info zur Zeitschrift: Publisher: W.B. Saunders Country of Publication: United States NLM ID: 0401126 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8240 (Electronic) Linking ISSN: 00313955 NLM ISO Abbreviation: Pediatr Clin North Am Subsets: MEDLINE

MeSH-Schlagworte: Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/therapy , Central Nervous System Infections*/microbiology , Pregnancy Complications, Infectious*/diagnosis, Humans ; Infant, Newborn ; Pregnancy ; Female ; Magnetic Resonance Imaging

Emergence of Parechovirus-A5 central nervous system infections in children from Kansas City, Missouri, USA.

Autoren: Dhar D Sasidharan A VanDonge KE et al.

Quelle: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2025 Oct; Vol. 180, pp. 105835. Date of Electronic Publication: 2025 Jul 15.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9815671 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5967 (Electronic) Linking ISSN: 13866532 NLM ISO Abbreviation: J Clin Virol Subsets: MEDLINE

MeSH-Schlagworte: Parechovirus*/genetics , Parechovirus*/classification , Parechovirus*/isolation & purification , Picornaviridae Infections*/epidemiology , Picornaviridae Infections*/virology , Picornaviridae Infections*/cerebrospinal fluid , Central Nervous System Infections*/epidemiology , Central Nervous System Infections*/virology , Communicable Diseases, Emerging*/epidemiology , Communicable Diseases, Emerging*/virology, Humans ; Infant ; Missouri/epidemiology ; Male ; Female ; Phylogeny ; Genotype ; Child, Preschool ; Infant, Newborn ; RNA, Viral/genetics

Cerebrospinal Fluid Metabolome in Central Nervous System Infections: A Study of Diagnostic Accuracy.

Autoren: Staal SL Olie SE van Weeghel M et al.

Körperschaften: Staal SL Olie SE van Weeghel M et al.

Quelle: Annals of neurology [Ann Neurol] 2025 Oct; Vol. 98 (4), pp. 851-863. Date of Electronic Publication: 2025 Jun 17.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7707449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-8249 (Electronic) Linking ISSN: 03645134 NLM ISO Abbreviation: Ann Neurol Subsets: MEDLINE

MeSH-Schlagworte: Central Nervous System Infections*/cerebrospinal fluid , Central Nervous System Infections*/diagnosis , Metabolome*/physiology, Humans ; Male ; Female ; Middle Aged ; Adult ; Aged ; Prospective Studies ; Metabolomics ; Cohort Studies ; Meningitis, Bacterial/cerebrospinal fluid ; Meningitis, Bacterial/diagnosis ; Young Adult ; Tandem Mass Spectrometry ; Netherlands

Evaluation of the Fujifilm Wako β-Glucan assay in cerebrospinal fluid for the diagnosis of non-cryptococcal fungal infections of the central nervous system.

Autoren: Samaddar A M S S N et al.

Quelle: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2025 Dec; Vol. 113 (4), pp. 117071. Date of Electronic Publication: 2025 Aug 18.

Publikationsart: Journal Article; Evaluation Study

Info zur Zeitschrift: Publisher: Elsevier Biomedical Country of Publication: United States NLM ID: 8305899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0070 (Electronic) Linking ISSN: 07328893 NLM ISO Abbreviation: Diagn Microbiol Infect Dis Subsets: MEDLINE

MeSH-Schlagworte: Mycoses*/diagnosis , beta-Glucans*/cerebrospinal fluid , Central Nervous System Infections*/diagnosis , Biological Assay*/methods, Humans ; Male ; Female ; Young Adult ; Adult ; Middle Aged

Utility of SIRS criteria and QSOFA score in identifying patients with central nervous system infections at risk for poor outcome.

Autoren: Resok YC Olie SE Jellema K et al.

Quelle: Scientific reports [Sci Rep] 2025 Oct 27; Vol. 15 (1), pp. 37447. Date of Electronic Publication: 2025 Oct 27.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH-Schlagworte: Systemic Inflammatory Response Syndrome*/diagnosis , Organ Dysfunction Scores* , Central Nervous System Infections*/diagnosis, Humans ; Female ; Male ; Middle Aged ; Intensive Care Units ; Aged ; Prospective Studies ; Meningitis, Bacterial/diagnosis ; Prognosis ; Adult

Central nervous system infections in patients with anti-interferon-γ autoantibodies: case series and review of the literature.

Autoren: Ning Y Liang H Liang S et al.

Quelle: BMC neurology [BMC Neurol] 2025 Aug 25; Vol. 25 (1), pp. 345. Date of Electronic Publication: 2025 Aug 25.

Publikationsart: Journal Article; Case Reports; Review

Info zur Zeitschrift: Publisher: BioMed Central Country of Publication: England NLM ID: 100968555 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2377 (Electronic) Linking ISSN: 14712377 NLM ISO Abbreviation: BMC Neurol Subsets: MEDLINE

MeSH-Schlagworte: Autoantibodies*/blood , Interferon-gamma*/immunology , Central Nervous System Infections*/immunology , Central Nervous System Infections*/blood, Humans ; Male ; Middle Aged ; Adult ; Female

Comparison of microbial culture, metagenomic next-generation sequencing and droplet digital polymerase chain reaction methods for pathogen detection in patients with neurosurgical central nervous system infection.

Autoren: Yu H Liang D Ding X et al.

Quelle: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2025 Jul 30; Vol. 15, pp. 1606283. Date of Electronic Publication: 2025 Jul 30 (Print Publication: 2025).

Publikationsart: Journal Article; Comparative Study

Info zur Zeitschrift: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE

MeSH-Schlagworte: High-Throughput Nucleotide Sequencing*/methods , Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/microbiology , Metagenomics*/methods , Bacteria*/genetics , Bacteria*/isolation & purification , Bacteria*/classification , Neurosurgical Procedures*/adverse effects , Polymerase Chain Reaction*/methods, Humans ; Male ; Female ; Middle Aged ; Adult ; Aged ; Young Adult ; Adolescent ; Sensitivity and Specificity

Pathogen Diagnostic Value of Nanopore Sequencing in Postoperative Central Nervous System Infections.

Autoren: Xie D Liu Y Cong W et al.

Quelle: World neurosurgery [World Neurosurg] 2025 Jul; Vol. 199, pp. 124090. Date of Electronic Publication: 2025 May 14.

Publikationsart: Journal Article

Info zur Zeitschrift: Publisher: Elsevier Country of Publication: United States NLM ID: 101528275 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-8769 (Electronic) Linking ISSN: 18788750 NLM ISO Abbreviation: World Neurosurg Subsets: MEDLINE

MeSH-Schlagworte: Nanopore Sequencing*/methods , Central Nervous System Infections*/diagnosis , Central Nervous System Infections*/microbiology , Central Nervous System Infections*/cerebrospinal fluid , Postoperative Complications*/diagnosis , Postoperative Complications*/microbiology, Humans ; Male ; Female ; Middle Aged ; Adult ; Aged ; High-Throughput Nucleotide Sequencing ; Young Adult ; Sensitivity and Specificity