Search Results - "BIOSYNTHESIS"

Multidimensional engineering strategies for strengthening exogenous synthesis of collagens and elastin monomers.

Authors: Liu LH Huang Y Chen R et al.

Source: International journal of biological macromolecules [Int J Biol Macromol] 2025 Dec; Vol. 334 (Pt 1), pp. 149075. Date of Electronic Publication: 2025 Nov 17.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE

MeSH Terms: Elastin*/biosynthesis , Elastin*/genetics , Elastin*/chemistry , Collagen*/biosynthesis , Collagen*/genetics , Protein Engineering*/methods , Collagen Type III*/genetics , Collagen Type III*/biosynthesis, Humans ; Protein Processing, Post-Translational ; Prolyl Hydroxylases/genetics ; Prolyl Hydroxylases/metabolism ; Mutation ; DNA-Directed RNA Polymerases/metabolism ; DNA-Directed RNA Polymerases/genetics ; Protein Biosynthesis

[Efficient expression and biological activity characterization of human potassium channel K V 3.1 in an Escherichia coli cell-free protein synthesis system].

Authors: Zhao Z Zhou T Song Y et al.

Source: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2025 Nov; Vol. 41 (11), pp. 1000-1006.

Publication Type: English Abstract; Journal Article

Journal Info: Publisher: Xi bao yu fen zi mian yi xue za zhi bian ji bu Country of Publication: China NLM ID: 101139110 Publication Model: Print Cited Medium: Print ISSN: 1007-8738 (Print) Linking ISSN: 10078738 NLM ISO Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Subsets: MEDLINE

MeSH Terms: Escherichia coli*/genetics , Escherichia coli*/metabolism , Shaw Potassium Channels*/genetics , Shaw Potassium Channels*/chemistry , Shaw Potassium Channels*/metabolism , Shaw Potassium Channels*/biosynthesis , Protein Biosynthesis*, Humans ; Cell-Free System ; Circular Dichroism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Recombinant Proteins/chemistry ; Recombinant Proteins/metabolism ; Membrane Potentials ; Shab Potassium Channels

The stringent response does not influence ribosome pausing in Bacillus subtilis.

Authors: Han Y Agnolin A van der Kloet F et al.

Source: Nucleic acids research [Nucleic Acids Res] 2025 Oct 28; Vol. 53 (20).

Publication Type: Journal Article

Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

MeSH Terms: Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , Ribosomes*/metabolism , Ribosomes*/genetics , Protein Biosynthesis*, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Tryptophan/biosynthesis ; Tryptophan/genetics ; Gene Expression Regulation, Bacterial ; alpha-Amylases/genetics ; alpha-Amylases/biosynthesis ; alpha-Amylases/metabolism ; Codon

Interconnectivity of mitochondrial protein biogenesis and quality control.

Authors: Ghifari AS Vazquez-Calvo C Carlström A et al.

Source: Trends in biochemical sciences [Trends Biochem Sci] 2025 Dec; Vol. 50 (12), pp. 1102-1117. Date of Electronic Publication: 2025 Oct 02.

Publication Type: Journal Article; Review

Journal Info: Publisher: Elsevier Trends Journals Country of Publication: England NLM ID: 7610674 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0968-0004 (Print) Linking ISSN: 09680004 NLM ISO Abbreviation: Trends Biochem Sci Subsets: MEDLINE

MeSH Terms: Mitochondrial Proteins*/metabolism , Mitochondrial Proteins*/biosynthesis , Mitochondria*/metabolism, Humans ; Animals ; Proteostasis ; Protein Biosynthesis ; Proteolysis

Treatment with L-type amino acid transporter 1 inhibitor JPH203 enhances protein synthesis in C2C12 myotubes.

Authors: Takegaki J Saneyasu T Honda K

Source: Scientific reports [Sci Rep] 2025 Nov 19; Vol. 15 (1), pp. 40805. Date of Electronic Publication: 2025 Nov 19.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

MeSH Terms: Muscle Fibers, Skeletal*/metabolism , Muscle Fibers, Skeletal*/drug effects , Large Neutral Amino Acid-Transporter 1*/metabolism , Protein Biosynthesis*/drug effects, Animals ; Mice ; Mechanistic Target of Rapamycin Complex 1/metabolism ; Cell Line ; TOR Serine-Threonine Kinases/metabolism ; Ribosomal Protein S6 Kinases, 70-kDa/metabolism ; Phosphorylation/drug effects ; Muscle Proteins/biosynthesis ; Benzoxazoles ; Tyrosine/analogs & derivatives ; Amino Acid Transport System y+L

The Adaptation of Cancer Cells to Serum Deprivation Is Mediated by mTOR-Dependent Cholesterol Synthesis.

Authors: Ilyassova B Rakhimgerey N Rakhimova S et al.

Source: International journal of molecular sciences [Int J Mol Sci] 2025 Nov 12; Vol. 26 (22). Date of Electronic Publication: 2025 Nov 12.

Publication Type: Journal Article

Journal Info: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

MeSH Terms: Cholesterol*/biosynthesis , TOR Serine-Threonine Kinases*/metabolism , TOR Serine-Threonine Kinases*/genetics , Adaptation, Physiological* , Neoplasms*/metabolism , Neoplasms*/genetics , Neoplasms*/pathology, Humans ; Cell Line, Tumor ; Culture Media, Serum-Free/pharmacology ; Gene Expression Regulation, Neoplastic ; Apoptosis/drug effects ; Protein Biosynthesis ; Mechanistic Target of Rapamycin Complex 1/metabolism ; Polyribosomes/metabolism

The Amount of Dietary Methionine Required to Maximize Synthesis of Transmethylated Products Is Higher than That Needed for Protein Synthesis in Neonatal Piglets.

Authors: Asiriwardhana MU Brunton JA Bertolo RF

Source: The Journal of nutrition [J Nutr] 2025 Nov; Vol. 155 (11), pp. 3896-3907. Date of Electronic Publication: 2025 Sep 24.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 0404243 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1541-6100 (Electronic) Linking ISSN: 00223166 NLM ISO Abbreviation: J Nutr Subsets: MEDLINE

MeSH Terms: Methionine*/administration & dosage , Methionine*/metabolism , Diet*/veterinary , Protein Biosynthesis*/drug effects, Animals ; Swine ; Animals, Newborn ; Animal Feed/analysis ; Creatine/metabolism ; Creatine/biosynthesis ; Nutritional Requirements ; Methylation ; DNA Methylation ; Animal Nutritional Physiological Phenomena ; Male

UBR5: A New Player in Protein Quality Control for Skeletal Muscle Growth and Remodeling.

Authors: Hughes DC Bodine SC

Source: Exercise and sport sciences reviews [Exerc Sport Sci Rev] 2025 Oct 01; Vol. 53 (4), pp. 205-213. Date of Electronic Publication: 2025 Sep 16.

Publication Type: Journal Article; Review

Journal Info: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0375434 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-3008 (Electronic) Linking ISSN: 00916331 NLM ISO Abbreviation: Exerc Sport Sci Rev Subsets: MEDLINE

MeSH Terms: Muscle, Skeletal*/growth & development , Muscle, Skeletal*/metabolism , Ubiquitin-Protein Ligases*/metabolism , Ubiquitin-Protein Ligases*/physiology , Muscle Proteins*/metabolism , Muscle Proteins*/biosynthesis , Muscle Development*/physiology, Humans ; Proteolysis ; Animals ; Hypertrophy ; Protein Biosynthesis

Recombinant expression and purification of wheat aminoacyl-tRNA synthetases and IRES-dependent polypeptide synthesis with homogeneously derived purified factors.

Authors: Furukawa H Yamagami R Nagashio Y et al.

Source: Biochimie [Biochimie] 2025 Sep; Vol. 236, pp. 30-44. Date of Electronic Publication: 2025 Jun 21.

Publication Type: Journal Article

Journal Info: Publisher: Editions Scientifiques Elsevier Country of Publication: France NLM ID: 1264604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1638-6183 (Electronic) Linking ISSN: 03009084 NLM ISO Abbreviation: Biochimie Subsets: MEDLINE

MeSH Terms: Triticum*/enzymology , Triticum*/genetics , Amino Acyl-tRNA Synthetases*/genetics , Amino Acyl-tRNA Synthetases*/isolation & purification , Amino Acyl-tRNA Synthetases*/biosynthesis , Amino Acyl-tRNA Synthetases*/metabolism , Internal Ribosome Entry Sites* , Plant Proteins*/genetics , Plant Proteins*/isolation & purification , Plant Proteins*/biosynthesis , Peptides*/genetics, Escherichia coli/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/genetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/metabolism ; Protein Biosynthesis

A Pichia pastoris Cell-Free Protein Synthesis Platform.

Authors: Aw R Alam F Spice AJ et al.

Source: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 2697, pp. 609-626.

Publication Type: Journal Article

Journal Info: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

MeSH Terms: Protein Biosynthesis* , Saccharomycetales*/metabolism , Saccharomycetales*/genetics, Cell-Free System ; Humans ; Plasmids/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/biosynthesis

SCR Organism: Komagataella pastoris

A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli.

Authors: Zhang J Yu K Xu Y et al.

Source: Nature communications [Nat Commun] 2025 Sep 29; Vol. 16 (1), pp. 8605. Date of Electronic Publication: 2025 Sep 29.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

MeSH Terms: Escherichia coli*/genetics , Escherichia coli*/metabolism , Genetic Code*/genetics , Amino Acids*/biosynthesis , Amino Acids*/genetics , Amino Acids, Aromatic*/biosynthesis , Amino Acids, Aromatic*/genetics, Biosynthetic Pathways/genetics ; Peptides/metabolism ; Protein Biosynthesis ; Metabolic Engineering/methods ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism

Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants.

Authors: Ghandour R Gao Y Ruf S et al.

Source: Nature plants [Nat Plants] 2025 Sep; Vol. 11 (9), pp. 1920-1938. Date of Electronic Publication: 2025 Aug 18.

Publication Type: Journal Article

Journal Info: Publisher: Nature Publishing Group, a division of Macmillan Publishers Limited Country of Publication: England NLM ID: 101651677 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2055-0278 (Electronic) Linking ISSN: 20550278 NLM ISO Abbreviation: Nat Plants Subsets: MEDLINE

MeSH Terms: Feedback, Physiological* , Photosynthetic Reaction Center Complex Proteins*/biosynthesis , Gene Expression Regulation, Plant* , Embryophyta*/metabolism, Photosynthesis ; Chlamydomonas reinhardtii/metabolism ; Ribulose-Bisphosphate Carboxylase/metabolism ; Protein Biosynthesis ; Proton-Translocating ATPases/metabolism

Autoregulation of YB-1 Synthesis in Cells.

Authors: Kachan VS Eliseeva IA Buyan AI et al.

Source: Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2025 Aug; Vol. 90 (8), pp. 1077-1087.

Publication Type: Journal Article

Journal Info: Publisher: MAIK Nauka/Interperiodica Country of Publication: United States NLM ID: 0376536 Publication Model: Print Cited Medium: Internet ISSN: 1608-3040 (Electronic) Linking ISSN: 00062979 NLM ISO Abbreviation: Biochemistry (Mosc) Subsets: MEDLINE

MeSH Terms: Y-Box-Binding Protein 1*/genetics , Y-Box-Binding Protein 1*/metabolism , Y-Box-Binding Protein 1*/biosynthesis, Humans ; Protein Biosynthesis ; RNA, Messenger/metabolism ; RNA, Messenger/genetics ; Homeostasis ; 3' Untranslated Regions ; 5' Untranslated Regions

OASL enhances mRNA translation and reprograms lipid metabolism to promote cancer progression.

Authors: Li SB Yuan L Zhu QY et al.

Source: Cell reports [Cell Rep] 2025 Jul 22; Vol. 44 (7), pp. 115901. Date of Electronic Publication: 2025 Jul 02.

Publication Type: Journal Article

Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE

MeSH Terms: Lipid Metabolism*/genetics , Protein Biosynthesis* , RNA, Messenger*/metabolism , RNA, Messenger*/genetics , Disease Progression* , Neoplasms*/pathology , Neoplasms*/genetics , Neoplasms*/metabolism , 2',5'-Oligoadenylate Synthetase*/metabolism , 2',5'-Oligoadenylate Synthetase*/genetics, Humans ; Animals ; Fatty Acids/metabolism ; Fatty Acids/biosynthesis ; Mice ; Cell Line, Tumor ; Ribosomes/metabolism ; Interferon Type I/metabolism ; Gene Expression Regulation, Neoplastic

Zika virus disrupts steroidogenesis and impairs spermatogenesis by stalling the translation of CYP17A1 mRNA.

Authors: Yang W Li H Wang S et al.

Source: Nature communications [Nat Commun] 2025 Jul 22; Vol. 16 (1), pp. 6756. Date of Electronic Publication: 2025 Jul 22.

Publication Type: Journal Article

Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

MeSH Terms: Spermatogenesis*/genetics , Steroid 17-alpha-Hydroxylase*/genetics , Steroid 17-alpha-Hydroxylase*/metabolism , Zika Virus*/genetics , Zika Virus*/metabolism , Zika Virus*/pathogenicity , Zika Virus*/physiology , Testosterone*/biosynthesis , Zika Virus Infection*/virology , Zika Virus Infection*/metabolism , Zika Virus Infection*/genetics , Zika Virus Infection*/pathology, Male ; Animals ; RNA, Messenger/metabolism ; RNA, Messenger/genetics ; Mice ; Humans ; Leydig Cells/metabolism ; Leydig Cells/virology ; Viral Nonstructural Proteins/metabolism ; Viral Nonstructural Proteins/genetics ; Protein Biosynthesis ; Testis/virology ; Testis/metabolism ; Testis/pathology ; Mice, Inbred C57BL

A molecular switch at the yeast mitoribosomal tunnel exit controls cytochrome b synthesis.

Authors: Carlström A Bridgers JB Couvillion M et al.

Source: Nucleic acids research [Nucleic Acids Res] 2025 Jul 08; Vol. 53 (13).

Publication Type: Journal Article

Journal Info: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

MeSH Terms: Saccharomyces cerevisiae Proteins*/metabolism , Saccharomyces cerevisiae Proteins*/genetics , Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae*/genetics , Cytochromes b*/biosynthesis , Cytochromes b*/genetics , Mitochondrial Proteins*/metabolism , Mitochondrial Proteins*/genetics , Mitochondrial Ribosomes*/metabolism, Protein Biosynthesis ; Mitochondria/metabolism ; Mitochondria/genetics ; RNA, Messenger/metabolism ; RNA, Messenger/genetics ; Gene Expression Regulation, Fungal ; Membrane Proteins ; Molecular Chaperones

Investigating muscle protein synthesis using deuterium oxide: The impact of dietary protein interventions across the lifespan.

Authors: Brook MS; Medical Research Council Versus Arthritis Centre for Musculoskeletal Ageing Research, University of Nottingham, Nottingham, UK.; NIHR Nottingham BRC, University of Nottingham, Nottingham, UK.; School of Life Sciences, University of Nottingham, Nottingham, UK.

Source: Experimental physiology [Exp Physiol] 2025 Jul; Vol. 110 (7), pp. 949-960. Date of Electronic Publication: 2025 Apr 24.

Publication Type: Journal Article; Review

Journal Info: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 9002940 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-445X (Electronic) Linking ISSN: 09580670 NLM ISO Abbreviation: Exp Physiol Subsets: MEDLINE

MeSH Terms: Dietary Proteins*/metabolism , Dietary Proteins*/administration & dosage , Muscle Proteins*/biosynthesis , Muscle, Skeletal*/metabolism , Deuterium Oxide*/metabolism , Longevity*/physiology, Humans ; Sarcopenia/metabolism ; Animals ; Aging/metabolism ; Aging/physiology ; Protein Biosynthesis

Development of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis.

Authors: Friedrich S Schramm M Kiebist J et al.

Source: Enzyme and microbial technology [Enzyme Microb Technol] 2025 Apr; Vol. 185, pp. 110588. Date of Electronic Publication: 2025 Jan 21.

Publication Type: Journal Article

Journal Info: Publisher: Elsevier Country of Publication: United States NLM ID: 8003761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0909 (Electronic) Linking ISSN: 01410229 NLM ISO Abbreviation: Enzyme Microb Technol Subsets: MEDLINE

MeSH Terms: Protein Biosynthesis* , Fungal Proteins*/genetics , Fungal Proteins*/biosynthesis , Fungi*/metabolism , Fungi*/genetics, Cell-Free System/metabolism ; Neurospora crassa/metabolism ; Neurospora crassa/genetics ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/metabolism ; Luciferases, Firefly/genetics ; Luciferases, Firefly/biosynthesis ; Genes, Reporter ; Culture Media

Developing an E. coli -Based Cell-Free Protein Synthesis System for Artificial Spidroin Production and Characterization.

Authors: Huang CY Wang RC Hsu TS et al.

Source: ACS synthetic biology [ACS Synth Biol] 2025 May 16; Vol. 14 (5), pp. 1829-1842. Date of Electronic Publication: 2025 Apr 21.

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

MeSH Terms: Fibroins*/genetics , Fibroins*/biosynthesis , Fibroins*/chemistry , Fibroins*/metabolism , Escherichia coli*/metabolism , Escherichia coli*/genetics, Cell-Free System/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/metabolism ; Animals ; Protein Biosynthesis ; Spiders/metabolism

Establishing a High-Yield Bacillus subtilis -Based Cell-Free Protein Synthesis System for In Vitro Prototyping and Natural Product Biosynthesis.

Authors: Ji X Liu WQ Cao Z et al.

Source: ACS synthetic biology [ACS Synth Biol] 2025 Apr 18; Vol. 14 (4), pp. 1288-1297. Date of Electronic Publication: 2025 Apr 09.

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal Info: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

MeSH Terms: Bacillus subtilis*/metabolism , Bacillus subtilis*/genetics , Protein Biosynthesis*/genetics , Biological Products*/metabolism, Cell-Free System/metabolism ; DNA-Directed RNA Polymerases/genetics ; DNA-Directed RNA Polymerases/metabolism ; Synthetic Biology/methods ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Promoter Regions, Genetic ; Recombinant Proteins/genetics ; Recombinant Proteins/biosynthesis